Chemical and Biological Technologies in Agriculture
·
2024
·Vol. 11
·期 1
·1-18
·
IF 5.2
Li Xiaojun,Chen Jiang,Yin Yuan,Xiao Shijun,Zhang Rui,Yang Yang,Li Le,Xu Hairong,Zhang Xue,Hu Pei
Background The Citrus reticulata Blanco peels have been widely adopted as functional food or nutraceuticals for thousands of years. In our study, two polysaccharides from the C. reticulata Blanco peels were obtained and named as CRP-WA and CRP-02A, respectively. After characterization by monosaccharide composition, methylation and NMR, chemical structures of CRP-WA and CRP-02A were elucidated. Results As a result, CRP-WA with Mw of 32.8 kDa was an arabinogalactan polysaccharide composed of (β1 → 3) (β1 → 3,6)-D-Gal p , (β1 → 4)-D-Man p and (β1 → 4)-D-Glc p units in the backbone. The CRP-02A with Mw of 70.5 kDa was an arabinogalacturonan type pectin formed by (α1 → 4) (α1 → 3,4)-D-Gal p A units in the backbone. Moreover, the surface morphologies and rheological properties of CRP-WA and CRP-02A were quite different from each other. Both of these two polysaccharides possessed good antioxidant activities and immunoregulatory effects on RAW 264.7 cells, in which CRP-02A behaved remarkable antioxidant feature, whereas CRP-WA showed relatively high immunoregulatory activity. Conclusion Our study will provide fundamental information for C. reticulata Blanco-based nutritional foods development. Graphical
研究领域:
食品化学
·
结构生物学
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药理学
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营养科学
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生物活性多糖
✓ 已验证
DOI
高影响因子
Nutrition & Diabetes
·
2024
·Vol. 14
·期 1
·1-9
·
IF 4.6
Li Hui,Fang Yujiao,Wang Da,Shi Bowen,Thompson Garth J.
Background Quantitative mapping of the brain’s metabolism is a critical tool in studying and diagnosing many conditions, from obesity to neurodegenerative diseases. In particular, noninvasive approaches are urgently required. Recently, there have been promising drug development approaches for the treatment of disorders related to glucose metabolism in the brain and, therefore, against obesity-associated diseases. One of the most important drug targets to emerge has been the Glucagon-like peptide-1 (GLP-1) and its receptor (GLP-1R). GLP and GLP-1R play an important role in regulating blood sugar and maintaining energy homeostasis. However, the macroscopic effects on brain metabolism and function due to the presence of GLP-1R are unclear. Methods To explore the physiological role of GLP-1R in mouse brain glucose metabolism, and its relationship to brain function, we used three methods. We used deuterium magnetic resonance spectroscopy (DMRS) to provide quantitative information about metabolic flux, fluorodeoxyglucose positron emission tomography (FDG-PET) to measure brain glucose metabolism, and resting state-functional MRI (rs-fMRI) to measure brain functional connectivity. We used these methods in both mice with complete GLP-1R knockout (GLP-1R KO) and wild-type C57BL/6N (WT) mice. Results The metabolic rate of GLP-1R KO mice was significantly slower than that of WT mice ( p = 0.0345, WT mice 0.02335 ± 0.057 mM/min, GLP-1R KO mice 0.01998 ± 0.07 mM/min). Quantification of the mean [ 18 F]FDG signal in the whole brain also showed significantly reduced glucose uptake in GLP-1R KO mice versus control mice ( p = 0.0314). Observing rs-fMRI, the functional brain connectivity in GLP-1R KO mice was significantly lower than that in the WT group ( p = 0.0032 for gFCD, p = 0.0002 for whole-brain correlation, p < 0.0001 for ALFF). Conclusions GLP-1R KO mice exhibit impaired brain glucose metabolism to high doses of exogenous glucose, and they also have reduced functional connectivity. This suggests that the GLP-1R KO mouse model may serve as a model for correlated metabolic and functional connectivity loss.
研究领域:
神经科学
·
代谢疾病
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药理学
·
成像技术
✓ 已验证
DOI
PubMed
高影响因子
NANO
·
2020
·
IF 1.566
Mei Wang,Fang Liu,Zhifeng Zhang,Erchao Meng,Feilong Gong,Feng Li
A new noninvasive glucose sensor is developed based on Co 3 O 4 particles (Co 3 O 4 NPs), which are synthesized by a single-step hydrothermal method with uniform structure and size. The electrochemical measurements reveal that the device exhibits outstanding performance for glucose detection, achieving a maximal sensitivity of 2495.79 μ A mM − 1 cm − 2 with a high R 2 of 0.99575, a ultra-low detection limit of 9.3 nM with a signal-to-noise of 3 and linear range up to 3 mM. The noninvasive glucose sensor can respond swiftly and selectively due to the high electrocatalytic activity of Co 3 O 4 NPs. The sensor also shows its high sensitivity and selectivity in detecting glucose levels in human blood serum and saliva sample, confirming the application potential of Co 3 O 4 NPs in noninvasive detection of glucose.
研究领域:
材料科学
·
电化学
·
纳米技术
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生物传感器
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生物医学工程
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Zhimin Luo,Lihui Yuwen,Yujie Han,Jing Tian,Xingrong Zhu,Lixing Weng,Lianhui Wang
Reduced graphene oxide/PAMAM–silver nanoparticles nanocomposite (RGO–PAMAM–Ag) was synthesized by self-assembly of carboxyl-terminated PAMAM dendrimer (PAMAM-G3.5) on graphene oxide (GO) as growing template, and in-situ reduction of both AgNO 3 and GO under microwave irradiation . The RGO–PAMAM–Ag nanocomposite was used as a novel immobilization matrix for glucose oxidase (GOD) and exhibited excellent direct electron transfer properties for GOD with the rate constant ( K s ) of 8.59 s −1 . The fabricated glucose biosensor based on GOD electrode modified with RGO–PAMAM–Ag nanocomposite displayed satisfactory analytical performance including high sensitivity (75.72 μA mM −1 cm −2 ), low detection limit (4.5 μM), an acceptable linear range from 0.032 mM to 1.89 mM, and also preventing the interference of some interfering species usually coexisting with glucose in human blood at the work potential of −0.25 V. These results indicated that RGO–PAMAM–Ag nanocomposite is a promising candidate material for high-performance glucose biosensors.
研究领域:
生物传感技术
·
材料科学
·
电化学
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Hai-Tao Ren,Chao-Chen Cai,Peng-Yue Zhu,Cong Wang,Song-Hai Wu,Yong Liu,Xu Han
Photocatalytic technology is a popular research area for converting solar energy into environmentally friendly chemicals and is considered the greenest approach for producing H2O2. However, the corresponding reactive oxygen species (ROS) and pathway involved in the photocatalytic generation of H2O2 by the Bi2.15WO6-glucose system are still not clear. Quenching experiments have established that neither •OH nor h+ contribute to the formation of H2O2, and show that the formed surface superoxo (≡Bi–OO•) and peroxo (≡Bi–OOH) species are the predominant ROS in H2O2 generation. In addition, various characterizations indicate the enhanced electron-transfer on the surface of Bi2.15WO6 with increasing contents of glucose via the ligand-to-metal charge transfer pathway, confirming H-transfer from glucose to ≡Bi–OO• or ≡Bi–OOH. The increased production of H2O2 with decreasing bond dissociation energy (BDEO–H) values of various phenolic compounds again supports the H-transfer mechanism from phenolic compounds to ≡Bi–OO• and then to ≡Bi–OOH. DFT calculations further reveal that on the Bi2.15WO6 surface, oxygen is sequentially reduced to ≡Bi–OO• and ≡Bi–OOH, while H-transfer from H2O or glucose to ≡Bi–OO• and ≡Bi–OOH, resulting in the production of H2O2. The lower energy barrier of H-transfer from adsorbed glucose (0.636 eV) than that from H2O (1.157 eV) indicates that H-transfer is more favorable from adsorbed glucose. This work gives new insight into the photocatalytic generation of H2O2 by Bi2.15WO6 in the presence of glucose/phenolic compounds via the H-abstraction pathway.
研究领域:
材料科学
·
环境科学
·
催化化学
✓ 已验证
DOI
PubMed
高影响因子
CELLULOSE
·
2024
·Vol. 31
·期 3
·1519-1532
·
IF 4.9
Zhu Yuan,Zhang Liyi,Qi Benkun,Luo Jianquan,Wan Yinhua
Acidified aqueous solutions of different quaternary ammonium salts were tested for pretreating corn stover compared to control solutions, including single acidic solution and acidified NaCl and NH 4 Cl solutions. The results revealed that quaternary ammonium salt solutions were more effective in delignification and cellulose saccharification than the control solutions. The chemical structure of these salts significantly influenced the pretreatment efficacy. Notably, benzyltrimethylammonium chloride (BTMAC), a cost-effective option, exhibited remarkable capabilities. It achieved approximately 90% xylan removal, 86% lignin removal, and a 48-h enzymatic glucose yield of up to 93%. The BTMAC solution proved to be recycled for at least four runs while maintaining satisfactory performance. This work contributes valuable insights into the promising potential of standalone QAS solutions, offering a viable and cost-effective option for biomass pretreatment.
研究领域:
生物化学
·
生物技术
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化学工程
·
可再生能源
·
环境科学
✓ 已验证
DOI
高影响因子
Applied Catalysis O: Open
·
2024
·
IF 0
Sijun Chen,Tingwei Zhang,Yongcan Jin,Huining Xiao
Fructose is a vital ketose in the production of 5-hydroxymethylfurfural platform molecules. Herein, Zr-doped hexagonal mesoporous silica was prepared over in situ co-assembly (Zr-x-HMS, x is the mole ratio of Si to Zr, x = 20, 50) and post-impregnation (Zr-Imx-HMS, x = 20, 50, 100) processes to regulate its catalytic performance against the isomerization of glucose to fructose in ethanol. The post-impregnation process did not significantly change the original pore structure and morphology of HMS, Zr-Im50-HMS with medium Zr loading possessed the most Lewis acid sites with medium strength among Zr-Imx-HMS catalysts. The in situ process changed the physical properties of HMS and enhanced the interaction of Zr sites and silica framework, facilitating the formation of Lewis acid sites with medium strength. Zr-Im50-HMS possessed the most suitable acidity for fructose production, and an optimal yield of 31.2% was achieved at 160 °C and 20 min. Moreover, the recyclability of Zr-Im50-HMS was acceptable.
研究领域:
催化
·
化学工程
·
材料科学
·
绿色化学
✓ 已验证
DOI
Frontiers of Materials Science
·
2025
·Vol. 19
·期 3
·1-12
·
IF 2.3
Li Guofu,Sun Yuhua,Dong Yuchi,Chen Di,Hu Jing,Sun Mizhen,Yuan Jinxiu,Zhou Ziyan,Xie Guoqiang,Feng Lijuan,Zhang Yingchao,Chen Yingjie,Yu Liyan,Dong Lifeng
Incorporating a secondary metal and engineering heterogeneous nanostructures can significantly enhance the electrochemical performance of non-enzymatic glucose sensors. Herein, NiCo 2 O 4 nanowire (NW) arrays were enveloped with NiCo 2 O 4 nanosheets (NSs) using a simple hydrothermal method. NSs improved the electronic conductivity of NW arrays, while arrays prevented the aggregation of NSs, resulting in exceptional glucose sensing performance. In 0.1 mol·L −1 NaOH electrolyte, this composite electrode demonstrated remarkable sensitivity (7641 µA·mmol −1 ·L·cm −2 ), broad linear detection range (1 to 1250 µmol·L −1 ), low detection limit (0.16 µmol·L −1 ), and rapid response time (within 1 s), as well as excellent selectivity, reproducibility, and stability. Such a unique architecture of NiCo 2 O 4 NW arrays enhanced the specific surface area of the composite material, facilitating the efficient electron transfer between copper foam and NiCo 2 O 4 NSs. This work provides an economical and efficient approach for developing enzyme-free glucose sensing catalysts with superior electrochemical properties.
研究领域:
电化学
·
纳米技术
·
分析化学
·
生物传感器
✓ 已验证
DOI
高影响因子
CyTA-Journal of Food
·
2018
·
IF 1.371
Yingmei Wu,Wenfeng Li,Yalong Lu,Qiu Wu,Xingbin Yang
High fructose (HF) ingestion is a common risk factor for the development of nonalcoholic fatty liver disease (NAFLD), which has become a serious health problem. The underlying mechanism of HF-induced NAFLD needs to be further understood and an effective prevention needs to be established urgently. Gas chromatography-mass spectrometer-based metabonomic method was employed to characterize the serum metabolic profile of HF-induced NAFLD in mice. β-Hydroxybutyric acid, elaidic acid and oleic acid that contributed to energy and lipid metabolism were confirmed as potential biomarkers for HF-induced NAFLD. With these changed metabolic pathways as possible drug targets, different administrations revealed that co-administration of stachyose and tea polyphenols was a more effective method than treatment of stachyose or tea polyphenols alone for preventing HF-induced negative changes of metabolic pathways in mice. These findings suggest that β-hydroxybutyric acid, elaidic acid and oleic acid are potential biomarkers in HF-induced NAFLD, and co-administration is a novel preventive strategy.
研究领域:
营养学
·
代谢组学
·
药理学
·
生物化学
·
食品科学
✓ 已验证
DOI
高影响因子
Bioresource Technology Reports
·
2020
·
IF 0
Kai Sun,Qingyin Li,Lijun Zhang,Yuewen Shao,Zhanming Zhang,Shu Zhang,Qing Liu,Yi Wang,Xun Hu
Polymerization is one major issue during acid-catalyzed conversion of sugars/furans to platform chemicals. Reaction medium is one of the main factors impacting the tendencies of sugars/furans towards polymerization. In this study, conversion of sugars (fructose/glucose/xylose/xylan) and furans (hydroxymethylfurfural (HMF)/furfural/furfuryl alcohol (FA)) over a solid acidic resin catalyst in various water-organic bi-solvents (toluene, chloroform , n-butanol, tetrahydrofuran (THF), dimethyl sulfoxide (DMSO) and ethyl acetate (EA)) were investigated. The results showed toluene/chloroform as the co -solvent did not suppress the polymerization of fructose, HMF, furfural and FA while diminished the formation of LA from fructose/HMF. The organic co-solvents remarkably affected properties of the coke formed. The presence of aprotic organic solvents tended to retain the C O group in the coke formed and affected its thermal stabilities and evolution of the functionalities. Additionally, polymerization tendency of the furans with the distinct structures showed very different sensitivity with the water in reaction medium.
研究领域:
化学工程
·
生物质转化
·
催化反应
✓ 已验证
DOI
JSFA Reports
·
2023
·Vol. 3
·期 3
·137-145
·
IF 0
Ruize Jiao,Yingyin Wu,Fangcheng Xu,Yan Li,Linchun Mao
Background Nuoshanyao (NSY) is a special species of Chinese yam ( Dioscorea opposita Thunb.) and shows rich functionality and superior nutritional value. The chemical components and structural properties of Nuoshanyao polysaccharide (NSYP) were investigated in his study. Results The molecular weight (Mw) of the major component in the polysaccharide was 23 kDa. NSYP was mainly composed of glucose, mannose, and galactose, and the molar ratio was 36.5:1.63:1. There might be an ordered helical conformation in NSYP, and the surface of NSYP was granular. NSYP exhibited strong scavenging capacity on the DPPH radical and chelating capacity on the ferrous ion in vitro with a rate of 94.94% and 82.49% at 4 mg/mL. Conclusion NSYP with the appropriate molecular weight range and helix structure could possess great antioxidant activity, while monosaccharide compositions may affect the activity. This work supplied important information for the potential application of NSYP in the nutritional and food industries.
研究领域:
食品科学
·
生物化学
·
营养学
✓ 已验证
DOI
Systems Microbiology and Biomanufacturing
·
2023
·
IF 0
Sun Tingting,Liu Di,Zhang Linmeng,Fan Taiping,Cai Yujie
(2R,3R)-BDO is an important bio-based four-carbon platform compound and widely used in chemical, food, pharmaceutical, fuel and aerospace fields . Serratia marcescens has been shown to be an effective strain for producing BDO. However, there have been few reports on the metabolic engineering of this strain to efficiently produce (2R,3R)-BDO using xylose. In this study, the endogenous strong promoter was screened and modified, and combined with the screened RBS. (2R,3R)-BDO was successfully produced by combining the selected promoters and RBS with the overexpressed key enzymes (ALS, ALSD, BDH, GLF) of (2R,3R)-BDO production. The optimum fermentation temperature and pH were 37 ℃ and 6.0, respectively, and the optimized yield reached 6.0 g/L. After batch fermentation, the (2R,3R)-BDO yield reached 36.6 g/L. This provided a good idea for efficient production of (2R,3R)-BDO from xylose.
研究领域:
生物工程
·
微生物学
·
生物化学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）