Food Science and Human Wellness
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2024
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IF 5.6
Yanan Liu,Huan Li,Chong Wang,Shanjun Chen,Renjie Lian,Weiqiang Wang,Linglin Fu,Yanbo Wang
Histamine in food has attracted widespread attention due to the potential toxicity and associated health risk. However, its influences on immunological components, especially the function of key immune cells, are still poorly known. In this work, we explored the effects of exogenous histamine on the function of key immune cells such as intestinal epithelial cells, dendritic cells, and T cells. The results showed that histamine could affect the expression of allergy-related genes in CMT93 cells at a high dose of histamine. Moreover, it’s found that histamine could cause an imbalance in the levels of relevant immune factors secreted by dendritic cells and T cells, especially those related to allergy. At the same time, the proportion of MHC class II-positive dendritic cells and the proportion of T helper 2 (Th2) cells in CD4 + T cells increased after histamine stimulation. We concluded that the presence of a certain level of histamine in food may affect the expression of allergy-related cytokines, disrupt the balance of the immune homeostasis, and potentially lead to adverse immune reactions. This work demonstrated the importance of including the estimation of histamine's immune safety in aquatic products rather than merely considering the potential risk of food poisoning.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xia Xu,Xinyu Wang,Yicheng Ding,Xuxia Zhou,Yuting Ding
Preserving fish meat poses a significant challenge due to its high protein and low fat content. This study introduces a novel approach that utilizes a common type of lanthanide metal-organic frameworks (Ln-MOFs), EuMOFs, in combination with 5-fluorescein isothiocyanate (FITC) and methylcellulose (MC) to develop fluorescent sensor arrays for real-time monitoring the freshness of fish meat. The EuMOF-FITC/MC fluorescence films were characterized with excellent fluorescence response, ideal morphology, good mechanical properties, and improved hydrophobicity. The efficacy of the fluorescence sensor array was evaluated by testing various concentrations of spoilage gases (such as ammonia, dimethylamine, and trimethylamine) within a 20-min timeframe using a smartphone-based camera obscura device. This sensor array enables the real-time monitoring of fish freshness, with the ability to preliminarily identify the freshness status of mackerel meat with the naked eye. Furthermore, the study employed four convolutional neural network (CNN) models to enhance the performance of freshness assessment, all of which achieved accuracy levels exceeding 93 %. Notably, the ResNext-101 model demonstrated a particularly high accuracy of 98.97 %. These results highlight the potential of the EuMOF-based fluorescence sensor array, in conjunction with the CNN model, as a reliable and accurate method for real-time monitoring the freshness of fish meat.
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TALANTA
·
2024
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IF 5.6
Jiahui Zou,Guancheng Zhu,Xueer Lin,Lanling Chu,Huimin Zhong,Cong Jiang,Yanyan Huang
Biogenic amines (BAs) are hazardous substances found in fishery products that are closely associated with fish spoilage and threaten food safety. Traditional chromatographic methods for detecting BAs are expensive, complex, and time-consuming. In this study, we developed a nanozyme-based sensor array to efficiently discriminate between four types of BAs and sensitively detect histamine. Copper-, cerium-, and manganese-based metal-organic frameworks with excellent peroxidase-like activities were employed as sensor elements. Because the catalytic activities of metal-organic frameworks could be modified by different BAs to varying degrees, the sensor array could generate a distinct colorimetric response pattern (fingerprint) for each BA. Based on this principle, the sensor array accurately discriminated BAs over a wide concentration range (10–1000 μM). Histamine could be distinguished down to 1 μM and detected with a detection limit of 4.28 μM within 20 min. In addition, mixtures of BAs, target BAs, interfering substances, and BAs in fishery product samples were well discriminated. Furthermore, our sensor array could also effectively distinguish the freshness of fish samples. This work might offer a useful strategy for the discrimination and detection of BAs and could positively contribute to food safety and public health.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Nian Shi,Wenxuan Li,Yuwei Liu,Shumei Yan,Xia Xu,Di Chen
The determination of biogenic amines (BAs) in alcoholic beverages is crucial for assessing their health impact, ensuring beverage quality, and guaranteeing safety. Herein, a rapid one-pot derivatization/magnetic solid-phase extraction (OPD/MSPE) method was proposed using 6-aminoquinolinyl-N-hydroxysuccinimide carbamate as the derivatization reagent and magnetic hydroxyl-functionalized multi-walled carbon nanotubes as the extraction material. Integration of derivatization and extraction steps simplifies the sample preparation process, taking only three minutes and eliminating the need for centrifugation by utilizing magnetic sorbent. The resulting desorption solution was directly analyzed by high-performance liquid chromatography-fluorescence detection (HPLC-FLD) without any evaporation or reconstitution steps. The integrated OPD/MSPE-HPLC-FLD method demonstrates excellent linearity (R 2 > 0.992), accuracy (relative recoveries: 85.1–109.2%), precision (RSDs≤9.7%) and detection limits (limits of detection: 0.3–2 ng/mL). It has been successfully applied to determine free BAs in various alcoholic beverages, including red wine, Baijiu, Huangjiu, and beer. This method enables rapid, sensitive and precise analysis of BAs in alcoholic beverages.
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TALANTA
·
2024
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IF 5.6
Yan Su,Pengfei Gao,Guomei Zhang,Ying Zhou,Lihong Shi,Jianlin Wu,Wenting Liang,Shaomin Shuang,Yan Zhang
In this study, the N-Acetyl- l -Cysteine (NAC)-capped gold and silver bimetallic nanoclusters (NAC@Au–Ag NCs) was synthesized by reflux method. Due to the silver effect, the NAC@Au–Ag NCs exhibited strong photoluminescence at far-red/near-infrared regions and better catalytic performance than Au or Ag NCs. Upon addition of H 2 S, the Au–Ag NCs exhibited obvious fluorescence quench and color changes through the generation of metal sulfides and a static quenching process. The Au–Ag NCs displayed a wide linear luminescence response for H 2 S (18.5–217 μmol/L) with a detection limit of 0.269 μmol/L. Moreover, the visible color of Au–Ag NCs changed from white to brown-yellow along with increased H 2 S, the corresponding RGB values also displayed good linearity with the concentration of H 2 S (90.1–678 μmol/L). Notably, the fabrication of test strips provided a convenient and intuitive tool to screen the freshness of eggs by the color change of test strips. Au–Ag NCs could be used for living HeLa cells bioimaging and recognition of H 2 S abnormalities. Furthermore, it can be used as a catalyst to reduction of nitrophenols (NPhs), specific included 2NP, 3NP and 4NP. The reaction was regarded as a pseudo-first-order kinetic reaction due to the presence of excess NaBH 4 . At 298K, the catalytic rate constants(k) of 2NP, 3NP and 4NP were 0.1754 min −1 , 0.1734 min −1 and 0.2782 min −1 , respectively. The NAC@Au–Ag NCs catalyst still showed good catalytic activity and reusability after five cycles. Therefore, this study developed a H 2 S sensor for food samples and biological systems. And this nanocatalyst had great application potential for removed the nitrophenol pollutants in water.
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BIOMACROMOLECULES
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2025
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IF 5.4
Mingyu Ding,Qianyu Jiang,Pengchao Wu,Pengliang Sui,Zichao Sun,Xiaoling Yang,Haibao Jin,Shaoliang Lin
Ultrathin organic nanofibers (UTONFs) have favorable potential as emerging nanomaterials due to their large aspect ratio, lightweight nature, and mechanical flexibility. Achieving dual stimuli-responsive UTONFs is necessary to satisfy the on-demand requirements of smart and miniature devices but remains challenging. Herein, amphiphilic alternating peptoids (AAPs) modified with azobenzene and histamine groups were successfully synthesized using the solid-phase submonomer synthesis technique. Following subsequent solution self-assembly, photo/CO2 dual-responsive ultrathin peptoid nanofibers (UTPNFs) with a diameter of ∼1.8 nm and a length of up to several micrometers were generated based on the pendant hydrophobic conjugate stacking mechanism. The photoisomerization of azobenzene was accountable for the reversible transformation from UTPNFs to spherical micelles (∼60 nm) under recyclable light irradiation. Owing to the protonation and the resulting electrostatic repulsion interaction, both UTPNFs and spherical micelles displayed a reversible variation in shape and physicochemical properties, including the size, diameter, zeta potential, and pH. Our work offers prospective guidance on the construction of dual-responsive ultrathin organic nanofibers with controllable shape transformation and performance transition.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Yong-Xiang Wu,Xun Zhou,Benwang Zhou,Chao Li,Peng Cui,Manhuan Cheng,Yang Wang,Xinsong She
Presently, Cu(OH) 2 nanowire arrays@Cu Foam (Cu(OH) 2 –NWA@Cu Foam) nanostructure has been confirmed to possess an excellent capacity of nonenzymatical electroanalysis of histamine (His). Based on Cu(OH) 2 –NWA@Cu Foam electrode, electrochemical investigations elucidated electron transfer kinetics principles of electrooxidation of His. Substantially, it was realized that a four–electron–transferring dehydrogenation was involved in diffusion–controlled electrooxidation of His, contributing tremendously to establishing as–proposed mechanization of His electrooxidation. At optimal conditions, Cu(OH) 2 –NWA@Cu Foam electrode reflected excellent electrochemical performance such as sensitivity of 3.089 mA mM –1 cm –2 , limit of determination of 94 nM, respond time of 1.6 s and linear respond rang of 0.5–1250 μM. The outperformed electroanalytical performance can be physically explicated by the dedications from larger exposed surface area, bigger interface–hydrophilicity–deduced His diffusion coefficient and less charge transfer resistance. Moreover, the outstanding selectivity, stability and reproducibility of Cu(OH) 2 –NWA@Cu Foam electrode ensured its practical effectiveness in regulating real His levels in fermented mandarin fish. Compared with control group, the mandarin fish by inoculating Lactobacillus sakei subsp . sakei delivered less His contents, deducing that inoculation of Lactobacillus sakei subsp . sakei can effectively depress His generation during fermentation process. Eventually, the one–step as–established Cu(OH) 2 –NWA@Cu Foam nanostructure positioned itself as an upcoming possibility to pursue commercialized utilization.
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FOOD CONTROL
·
2024
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IF 5.6
Jianli Zhou,Junjun Wang,Xiaohuan Huang,Lian Xia,Han Tao,Yuangen Wu
The colorimetric methods for histamine detection still face some challenges in accuracy and stability. Herein, a novel colorimetric aptasensor for sensitive and highly selective detection of histamine was developed based on the enhancement of silver citrate (AgCit) laccase-mimic activity. It has been confirmed that H2 aptamer enhances the affinity of AgCit to the chromogenic substrate, and promotes the generation of semiquinone radical in the laccase-mimic catalytical system. The color change of the sensing solution can be distinguished by the naked eye, and easily converted to the corresponding RGB value by a smartphone for the quantitative and rapid detection of histamine. The aptasensor has a limit of detection (LOD) as low as 27 μg L −1 , and maintains high selectivity for histamine in the presence of other competitive substances. It is strongly believed that the design of a colorimetric aptasensor can provide a simple and versatile platform for histamine detection in food.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2024
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IF 3.3
Xinli Peng,Siqi Wang,Kaiyue Su,Yufeng Sun,Zhixiang Xu
BACKGROUND Histamine may lead to low blood pressure, skin flushing and edema when it accumulates in large amounts in the body. Therefore, establishing sensitive methods for the detection of histamine in foods is extremely important to ensure food safety and human health. RESULTS The MI-Cu-GMP NPs (2-methylimidazole-copper-guanosine monophosphate nanozymes) with high laccase-like activity were synthesized. Using the prepared molecular imprinted membrane as biomimetic antibody and MI-Cu-GMP NPs as marker, a sensitive direct competitive biomimetic enzyme-linked immunoassay (BELISA) method for rapid detection of the histamine in foods was developed. Under optimal conditions, the limit of detection (LOD, IC 15 ) and sensitivity (IC 50 ) of the BELISA method for histamine was 0.05 mg L −1 and 1.22 mg L −1 , respectively. The liquor samples spiked with histamine was detected by the BELISA method with satisfactory recoveries ranging from 90.00% to 116.00%. Further, the level of histamine in three samples (cooking wine, rice vinegar and soy sauce) was tested by the BELISA and high-performance liquid chromatography (HPLC), with no significant difference found between the two methods. CONCLUSION Given the advantages, the established BELISA method is expected to provide practical guidance for the monitoring of histamine in food and provides a foundation for the detection of other food hazards. © 2024 Society of Chemical Industry.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Chengtuo Niu,Yiyang Liu,Heng Li,Chunfeng Liu,Qi Li
This study aimed to elaborate the biochemical and chemosensory characteristics of doubanjiang fermentation via a two-stage controlled temperature. HL group with variable temperature (40 °C → 30 °C) and NTF group fermented following traditional technique were prepared and their volatile and non-volatile metabolites were compared through multivariate statistical analysis. HL group favored the accumulation of amino acid nitrogen, free amino acids and organic acids in the early stage and maintained adequate total acids and biogenic amines in the mid-late stage. HL group also had preferred jiang and fruity flavor through sensory evaluation. A total of 116 volatile metabolites were identified in HL or NTF groups and 22 marker volatile metabolites were screened through the combinational use of OPLS-DA and Random Forest analysis. Stronger anti-oxidant ability was observed in HL group while adequate number of acidic compounds and biogenic amines were ensured. This indicated that the two-stage controlled temperature fermentation was beneficial for doubanjiang fermentation.
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BIOSENSORS & BIOELECTRONICS
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2024
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IF 10.7
Donglei Jiang,Zeng Feng,Hui Jiang,Xinyue Xiang,Lifeng Wang
An innovative integrated three-dimensional (3D) bioprinted gastric microtissue electrochemical biosensor was developed in this study for the detection of allergen ovalbumin (OVA). In this system, OVA triggers the release of histamine from gastric microtissue, which then undergoes a redox reaction on the electrode surface, leading to an increase in the peak current. Gelatin methacrylate hydrogel serves as a scaffold for the 3D culture of RBL-2H3 and PC-12 cells for partially restoring allergic reactions in the human body in vitro. Furthermore, gold nanoparticle-modified anodized aluminum oxide sieves macromolecular substances and facilitates sensor nano-analysis. Composites of cerium-based organometallic framework, MnO 2 , and gold nanoparticles significantly enhanced the sensitivity of the screen-printed carbon electrode. Under optimal experimental conditions, the detection limit for OVA was 0.042 μg/mL, with a linear range of 0.1–10.0 μg/mL. The fabricated sensor demonstrated high sensitivity, reliability, and simplicity, showcasing its broad potential for allergen detection applications.
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FOOD CHEMISTRY
·
2024
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IF 8.5
Tiantian Zhang,Haonan Mi,Shichao Wei,Yuan Xu,Zhao Yang,Shuai Liang,Xuelu Ding
A novel, compact, and automated laser ablation dielectric barrier discharge thin layer chromatography-mass spectrometry (LA-DBD-TLC-MS) device was developed for the rapid detection of biogenic amines (BAs) in fishery products. This plug-and-play system integrates thermal desorption via diode laser, DBD plasma ionization, and tandem MS detection, with key operational parameters optimized through experimental and computational methods. Utilizing nanoscale carbon black as a matrix, the device achieved a detection limit of 0.230 pg/mm 2 . It demonstrated comparable efficacy to HPLC-MS/MS in screening six BAs and enabled species discrimination and spoilage assessment in fish with machine learning algorithms (PCA, RF, SVM, MLP), yielding high accuracies. A multi-linear regression model predicted salmon spoilage with a strong correlation (R 2 = 0.974). This study confirms the LA-DBD-TLC-MS device as a reliable, precise, and high throughput tool for rapid BA analysis in fishery products, with potential for direct trace analysis of organic compounds in food matrices.
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