JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Chen Yan,Na Li,Yuchi Zhang,Yun Wei
Cinnamaldehyde has been widely applied in various fields due to its special flavor and various pharmacological activities, such as antioxidant, anti-inflammatory and antibacterial properties. The strategy of quick and efficient enrichment for cinnamaldehyde is imperative. In this study, an electroosmotic coupled particle-assisted solvent flotation (ECPASF) system was designed for the cinnamaldehyde enrichment from cinnamon. The response surface method was used to optimize extraction parameters. Under optimal operating conditions, its yield was 9.33 ± 0.11 mg/g. Such high yield of cinnamaldehyde using the ECPASF might be because electroosmosis effectively alters the permeability of plant cells, which facilitates the release of cinnamaldehyde. In addition, both the crude extract of cinnamon and pure cinnamaldehyde showed good antioxidant activity. The results demonstrated that the ECPASF system is a sustainable and effective method for the extraction of cinnamaldehyde from cinnamon. It also has the prospect of being extended to the extraction of other natural products.
研究领域:
药理学
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化学工程
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食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Xiaolin Hou,Meng Long,Nianping Feng,Yongtai Zhang
Low solubility, poor physicochemical stability and unpleasant odor are the main limitations for the application of some essential oils in the pharmaceutical and food industries. In this work, cinnamon oil (CIO) was encapsulated as oil phase with soya phospholipids as emulsifiers in sodium alginate-formed nanocapsules (NCs), which were further reinforced with acid-processed gelatin by electrostatic adsorption, and then incorporated into hyaluronic acid (HA)-fabricated microneedles (MNs) to construct a new transdermal drug delivery system (HAMNs-CIO@NCs). The obtained CIO-loaded nanocapsules (CIO@NCs) with optimized formulation showed 246.5 ± 4.2 nm in diameter, −24.5 ± 1.7 mV in zeta potential , 22.60% in drug loading, and kept physical stability. In HAMNs-CIO@NCs, the CIO@NCs were homogeneously distributed, and the HAMNs-CIO@NCs with sufficient mechanical hardness were successfully pierced into skin. Compared with the free CIO application, the transdermal fluxes of cinnamaldehyde and cinnamic acid in HAMNs-CIO@NCs group were increased to 8.5 and 6.3 times, and the application was completed within 1 min with no essential oil residue on skin, which improved patient compliance. Further more, HAMNs-CIO@NCs effectively alleviated the symptoms of primary dysmenorrhea in rats and reduced the expression ratio of PGF 2α /PGE 2 in uterus. This newly designed drug delivery system is formulated with natural food-derived materials, and CIO is served as drug and carrier excipient for self-assembling nanocapsules in a green manufacturing process, thus providing a new strategy for efficiently using essential oils and contributing to a simple and safe treatment for relieving pain of primary dysmenorrhoea.
研究领域:
药学
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药物递送系统
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透皮技术
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天然产物化学
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疼痛管理
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产品关联
DOI
高影响因子
FOOD CONTROL
·
2025
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IF 6.3
Yingshuang Lu,Yu Jiang,Mengyu Sun,Lu Dong,Xiaolong Xing,Yan Zhang,Shuo Wang
This study explores the formation of advanced glycation end products (AGEs) in chicken breast during roasting, focusing on the analytical characterization and simulation of dynamic models for the content of carboxymethyllysine (CML) and carboxyethyllysine (CEL), two key AGEs. To systematically investigate the formation patterns of AGEs, the chicken breasts were subjected to five roasting temperatures ranging from 180 °C to 230 °C. At each temperature, samples were roasted to achieve five distinct core temperatures, from 60 °C to 100 °C. Dynamic models for CML and CEL content were constructed using multiple linear regression and partial least squares methods, with the best-fit models identified based on their R 2 and RMSE values, with R 2 values of 0.886 for CEL, 0.954 for CML, and RMSE values indicating minimal prediction error. Our research provides a robust framework for predicting AGEs formation during roasting with validation, offering valuable insights for optimizing cooking processes to balance product quality and safety.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
BIOMEDICAL CHROMATOGRAPHY
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2020
·Vol. 35
·期 3
·e4991
·
IF 1.728
Li-Juan Nie,Jun Liang,Feng Shan,Ya-Yun Xu,Chun-Yu Yan,Xuan Zhou,Zhuo-Yu Cheng,Qing-Rong Xia
A simple, rapid, and selective ultra-performance liquid chromatography–tandem mass spectrometry method for determination of l -carnitine (LC) and acetyl- l- carnitine (ALC) in human serum was developed. Acetyl- l- carnitine-d 3 (ALC-d 3 ) was selected as internal standard (IS). After protein precipitation with acetonitrile–water (1 mL, 2:1, v/v), the analytes and IS were separated on a 2.5-μm XSelect HSS T3 C 18 column by gradient elution with methanol–water (containing 0.01% ammonia water) as the mobile phase at a flow rate of 0.2 mL/min. Analytes were detected with multiple reaction monitoring using a positive scan mode with electrospray ionization. Good linearity ( R 2 > 0.999) was observed in the concentration range for LC and ALC. The inter- and intra-day values of relative error were −10.4% to 10.0% with CVs less than 9.84%. The average recoveries of the two analytes were 91.29%–98.23%. Blood samples containing LC and ALC were stable under various storage conditions. Normal, haemolytic, and hyperlipidaemic serum had no significant effect on the quantification of LC and ALC. This method was successfully applied to study the concentrations of endogenous LC and ALC in the serum of patients with first-episode depression.
研究领域:
分析化学
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医学
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精神健康研究
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 20
·3814
·
IF 5.2
Jiahang Yu,Mingyuan Huang,Huixin Tian,Xinglian Xu
The series of biochemical reactions, metabolic pathways, and regulatory interactions that occur during the storage of meat are the main causes of meat loss and waste. The volatile compounds produced by these reactions, such as hydrogen sulfide, acids, and amines, can directly indicate changes in the freshness of meat during storage and sales. In this study, a one-pot hydrothermal method based on a surface control strategy was used to develop nanoparticles of silver with different reactivities, which were further immobilized in agar powder to develop a colorimetric sensor array. Due to the different chemical interactions with various volatile compounds, the colorimetric sensor array exhibited distinct color changes. The study demonstrates significant differences between 12 different volatile compounds and provides a quantitative and visual method to reveal rich detection indicators. The colorimetric sensor array is an economical and practical multi-analyte identification method. It has many potential applications such as food packaging, anti-counterfeiting, health monitoring, environmental monitoring, and optical filters.Keywords:colorimetric sensor array;Ag NPs;VOCs;nanosensors;chilled broiler meat
研究领域:
食品科学
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纳米技术
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生物传感
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DOI
PubMed
高影响因子
POULTRY SCIENCE
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2024
·
IF 3.8
Xin Yang,Bolin Cai,Zhaofeng Zhang,Yu Mo,Zhen Zhou,Ruiquan Wu,Shaofen Kong,Danfeng Cai,Ruitong Zhang,Zhenhui Li,Qinghua Nie
Previously, animal breeding prioritized enhancing key economic traits to improve production efficiency, leading to a gradual difference in meat quality. However, the genetic factors influencing meat quality remain unclear. To identify key genetic pathways contributing to meat quality, native Chinese yellow-feathered chicken (Qingyuan Partridge Chicken, QPC; female, n=10), and commercial chicken broiler (Cobb broiler, CB; female, n=10) were used for meat quality assessment through metabolomics, proteomics, and phosphoproteomics sequencing. The results show that QPC had lower pH (93.12%), shear force (81.46%), cooking loss (69.29%), moisture content (93.24%) and muscle fiber area (46.04%), but higher meat color values (a*(163.65%) and b*(250.27%)), drip loss (146.32%), and intramuscular fat content (382.01%) than CB ( p < 0.05). Metabolomic, proteomic, and phosphoproteomic analyses were jointly conducted, revealing significant differences in energy metabolism strategies. Higher glycolytic enzyme activity was observed in QPC (ENO1, GAPDH, GPI, PFKM, PKM, and TPI1, p < 0.05), while more energetic phosphate compounds were stored in CB. CB had higher Na + /K + Pump protein abundance (SCN4A, LOC107051305, ATP1B4, ATP12A, ATP1A1, and ATP1A2, p < 0.05) and phosphorylation (ATP1A2-Ser662, p < 0.05) and Ca 2+ channel protein abundance (ATP2B4, SRL, CACNB1, CACNA1S, CACNA2D1, CAMK2G, LOC107050717 and TNNC2, p < 0.05) than QPC. In QPC, CAMKII autophosphorylation activated downstream protein and increased Ca 2+ . These results suggest CB is more contractile than QPC, contributing to meat quality between CB and QPC.
研究领域:
动物遗传育种
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食品科学
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生物信息学
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DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
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2024
·
IF 11
Chao Qiu,Baicun Chen,Wenqi Yin,David Julian McClements,Zhengyu Jin,Hangyan Ji
Pure chitosan films are relatively fragile and tend to dissolve when they come into contact with water, which limits their application in food preservation. In this study, cinnamaldehyde-tannic acid-zinc acetate (CTZA) nanoparticles and aldehyde crosslinking were used to enhance the mechanical and water barrier properties of chitosan films. Fourier transform infrared spectroscopy and X-ray diffraction analyses showed that cinnamaldehyde had been successfully grafted on the surfaces of chitosan films. The thermal stability and mechanical property of the chitosan films were greatly improved after these modifications. Compared to non-modified films, the tensile strength of modified films increased by about 1.5- and 8.2-fold under dry and wet conditions, respectively. In addition, the moisture content, swelling degree, water solubility, and water vapor permeability of the modified films were all significantly decreased after the aldehyde modification. Interestingly, the modified film showed no significant changes in dimensions after being immersed in water for 24 h. Moreover, the UV-blocking properties and antioxidant activity of the modified films were significantly enhanced. Finally, the modified films extended the shelf life of packaged beef by 5 d. These findings suggest that the modified chitosan films can be used as active packaging materials for food preservation.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shuofeng Li,Qianqian Wang,Yixuanfei Gao,Yihang He,Zhi Wang,Chun Wang,Qiuhua Wu
Herein, a novel chitosan based hyper crosslinked polymer (Chi-gs-BDCB) was synthesized as sorbent by crosslinking reaction with biphenyl dichlorobenzyl as crosslinking agent after grafting chitosan with styrene. Benefiting from large specific surface area, affluent hydroxyl-functional groups, high porosity and good hydrophilicity, Chi-gs-BDCB displayed good adsorption properties for nitroimidazole antibiotics. Applying Chi-gs-BDCB as solid-phase extraction sorbent coupled with high performance liquid chromatography-ultraviolet detection, a sensitive and accurate analytical method for detecting nitroimidazole antibiotics in beef and mutton meat was established. Under optimal conditions and at S/N = 3, high sensitivity was obtained with the detection limits of 0.40–1.60 ng g −1 for mutton and 0.33–1.0 ng g −1 for beef. Satisfactory accuracy and precision were confirmed by the recoveries of 81.4 %–116 % and low relative standard deviations of 1.23 %–10.0 %. These data validate the feasibility and effectiveness of the chitosan-based sorbent for enriching nitroimidazole antibiotics at trace level from beef and mutton meat.
研究领域:
食品科学
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分析化学
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环境科学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
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2023
·Vol. 103
·期 5
·2367-2377
·
IF 4.1
Shun Chen,HuiJuan Liu,JiaQi Zhang,BinBin Zhou,XiaoFang He,Tian Wang,Chao Wang
BACKGROUND This study was conducted to investigate the effect of dietary rutin on the meat quality, antioxidant status and mitochondrial structure and function in the breast muscle of heat-stressed broilers. A total of 192 male broilers were randomly assigned into three groups and treated with normal control (CON), heat stress (34 °C, HS), and HS with 500 mg kg −1 rutin supplementation (HS + Rutin), respectively. RESULTS Dietary rutin significantly reversed HS-induced decrease in body weight, average daily feed intake, average daily gain, and feed efficiency. Rutin supplementation attenuated HS-induced impaired meat quality by decreasing the lightness, drip loss at 24 and 48 h, the peak time of free water ( T 22 ) and the peak area ratio of free water ( P 22 ), and increasing the pH 24h and peak area ratio of immobilized water ( P 21 ). Rutin supplementation promoted superoxide dismutase, glutathione peroxidase activities and total antioxidant capacity, and decreased malondialdehyde levels compared with the HS group. Moreover, rutin attenuated HS-induced mitochondrial damage by increasing the mitochondrial DNA copy number and improving mitochondrial morphology. Dietary rutin significantly increased mitochondrial biogenesis-related mRNA (proliferator-activated γ receptor coactivator-1α [PGC-1α], nuclear respiratory factor 1 [NRF1], and mitochondrial transcription factor A [TFAM]) expression via the AMP-activated protein kinase (AMPK) signaling pathway. HS significantly increased mitophagy-related genes and proteins (Parkin, PTEN-induced putative kinase 1 [PINK1], microtubule associated protein light chain 3-II [LC3-II]) expression, and dietary rutin significantly reversed these alterations. CONCLUSION Dietary rutin attenuated the HS-induced decline in meat quality and antioxidant capacity of broilers, which may be related to inhibition of the AMPK/PINK1–Parkin signaling pathway to attenuate mitochondrial damage. © 2023 Society of Chemical Industry.
研究领域:
动物科学
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营养学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2023
·
IF 5.5
Jiapeng Deng,Su Liu,Guoqing Li,Yien Zheng,Weifei Zhang,Jianjing Lin,Fei Yu,Jian Weng,Peng Liu,Hui Zeng
Introduction: Chemotherapy is a common strategy for the treatment of osteosarcoma. However, its therapeutic efficacy is not ideal due to the low targeting, lowbioavailability, and high toxicity of chemotherapy drugs. Nanoparticles can improve the residence time of drugs at tumor sites through targeted delivery. This new technology can reduce the risk to patients and improve survival rates. To achieve this goal, we developed a pHsensitive charge-conversion polymeric micelle [mPEG- b -P(C7- co -CA) micelles] for osteosarcoma-targeted delivery of cinnamaldehyde (CA). Methods: First, an amphiphilic cinnamaldehyde polymeric prodrug [mPEG- b -P(C7- co -CA)] was synthesized through Reversible Addition-Fragmentation Chain Transfer Polymerization (RAFT) polymerization and post-modification, and self-assembled into mPEG- b -P(C7- co -CA) micelles in an aqueous solution. The physical properties of mPEG- b -P(C7- co -CA) micelles, such as critical micelle concentration (CMC), size, appearance, and Zeta potential were characterized. The CA release curve of mPEG- b -P(C7- co -CA) micelles at pH 7.4, 6.5 and 4.0 was studied by dialysis method, then the targeting ability of mPEG- b -P(C7- co -CA) micelles to osteosarcoma 143B cells in acidic environment (pH 6.5) was explored by cellular uptakeassay. The antitumor effect of mPEG- b -P(C7- co -CA) micelles on 143B cells in vitro was studied by MTT method, and the level of reactive oxygen species (ROS) in 143B cells after mPEG- b -P(C7- co -CA) micelles treatment was detected. Finally, the effects of mPEG- b -P(C7- co -CA) micelles on the apoptosis of 143B cells were detected by flow cytometry and TUNEL assay. Results: An amphiphilic cinnamaldehyde polymeric prodrug [mPEG- b -P(C7- co -CA)] was successfully synthesized and self-assembled into spheric micelles with a diameter of 227 nm. The CMC value of mPEG- b -P(C7- co -CA) micelles was 25.2 mg/L, and it showed a pH dependent release behavior of CA. mPEG- b -P(C7- co -CA) micelles can achieve chargeconversion from a neutral to a positive charge with decreasing pHs. This charge-conversion property allows mPEG- b -P(C7- co -CA) micelles to achieve 143B cell targeting at pH 6.5. In addition, mPEG- b -P(C7- co -CA) micelles present high antitumor efficacy and intracellular ROS generation at pH 6.5 which can induce 143B cell apoptosis. Discussion: mPEG- b -P(C7- co -CA) micelles can achieve osteosarcoma targeting effectively and enhance the anti-osteosarcoma effect of cinnamaldehyde in vitro . This research provides a promising drug delivery system for clinical application and tumor treatment.
研究领域:
药学
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纳米技术
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肿瘤学
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药物递送系统
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2017
·Vol. 24
·期 10
·9379-9386
·
IF 2.741
Jia Yuyu,Wang Lin,Qu Zhipeng,Wang Chaoyi,Yang Zhaoguang
Three fish species ( Carassius auratus , Pelteobagrus fulvidraco , and Squaliobarbus curriculus ) were collected from Xiang River near Changsha City, Southern China. The concentrations of heavy metals including arsenic (As), cadmium (Cd), copper (Cu), iron (Fe), manganese (Mn), lead (Pb), and zinc (Zn) in the muscle, gill, and liver of three species were determined by the inductively coupled plasma mass spectrometry method. One-way analysis of variance (ANOVA) was introduced to determine the significant variations ( p < 0.05) of heavy metals. Livers were found to accumulate Cd and Cu due to the metallothionein proteins. High levels of Mn and Pb in the gills indicated that the main uptake pathway of these heavy metals was from the water. The carnivorous species, P. fulvidraco , was found to accumulate the highest levels of toxic elements (As, Cd, and Pb), while relatively high concentrations of nutrient elements (Cu, Fe, Mn, and Zn) were accumulated in omnivorous species ( C. auratus and S. curriculus ). According to the results of Pearson’s correlation analysis, there were few significant relationships at p < 0.01 level between the concentrations of the analyzed elements and the fish sizes. The results of risk assessment indicated that exposure to the toxic heavy metals from fish muscle consumption posed no non-carcinogenic health risk to local inhabitants.
研究领域:
生态毒理学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Cell Communication and Signaling
·
2023
·Vol. 21
·期 1
·1-18
·
IF 8.4
Liao Zhaohong,Lan Haiqiang,Jian Xiaoting,Huang Jingwen,Wang Han,Hu Jijie,Liao Hua
Background To explore the role of skeletal muscle specific TGF-β signaling on macrophages efferocytosis in inflamed muscle caused by Cardiotoxin (CTX) injection. Methods CTX myoinjury was manipulated in TGF-βr2 flox/flox (control) mice or transgenic mice with TGF-β receptor 2 (TGF-βr2) being specifically deleted in skeletal muscle (SM TGF-βr2 −/− ). Gene levels of TGF-β signal molecules, special inflammatory mediators in damaged muscle or in cultured and differentiated myogenic precursor cells (MPC-myotubes) were monitored by transcriptome microarray or qRT-PCR. TGF-β pathway molecules, myokines and embryonic myosin heavy chain in regenerating myofibers, the phenotype and efferocytosis of macrophages were evaluated by immunofluorescence, immunoblotting, Luminex, or FACS analysis. In vitro apoptotic cells were prepared by UV-irradiation. Results In control mice, TGF-β-Smad2/3 signaling were significantly up-regulated in regenerating centronuclear myofibers after CTX-myoinjury. More severe muscle inflammation was caused by the deficiency of muscle TGF-β signaling, with the increased number of M1, but the decreased number of M2 macrophages. Notably, the deficiency of TGF-β signaling in myofibers dramatically affected on the ability of macrophages to conduct efferocytosis, marked by the decreased number of Annexin-V − F4/80 + Tunel + macrophages in inflamed muscle, and the impaired uptake of macrophages to PKH67 + apoptotic cells transferred into damaged muscle. Further, our study suggested that, the intrinsic TGF-β signaling directed IL-10-Vav1-Rac1 efferocytosis signaling in muscle macrophages. Conclusions Our data demonstrate that muscle inflammation can be suppressed potentially by activating the intrinsic TGF-β signaling in myofibers to promote IL-10 dependent-macrophages efferocytosis.
Video Abstract
研究领域:
免疫学
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肌肉生物学
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细胞信号传导
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炎症
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）