ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Muhammad Saqib,Shahida Bashir,Haijuan Li,ShanShan Wang,Yongdong Jin
Development of simple and effective chemiluminescence (CL) systems for multiple sensing applications is significantly important but still a challenge. Until now, the majority of CL systems primarily utilized hydrogen peroxide (H2O2) as coreactant, which is limited in its stability and selectivity due to the easy decomposition of H2O2 in the presence of several ions. In this study, we develop a new and intense CL system by combined use of tris(2-carboxyethyl)phosphine (TCEP), a highly solution stable and pH-tolerant tertiary phosphine, with lucigenin for the first time. The effective pairing leads to a significant ∼23 times CL enhancement over classic the lucigenin–H2O2 system without employing additional catalysts. By virtue of this fascinating platform, a sensitive CL method has been developed for the multiple detection of TCEP (LOD = 70 nM), lucigenin (LOD = 4.0 nM), superoxide dismutase (LOD = 0.8 ng/mL), Hg2+(LOD = 0.3 nM), and dopamine (LOD = 3.0 nM), with a linear range of 0.1–320 μM, 0.01–55 μM, 0.005–0.5 μg/mL, 1.0–600 nM, and 0.01–0.8 μM, respectively. Remarkably, this CL method exhibited superior selectivity over several potential interferents. Moreover, the proposed method achieved excellent recoveries in the range of 94.0–102.3% for both Hg2+ detection in lake water and dopamine detection in human serum real samples. We envision that broad applications of TCEP may lead to construct new CL systems, pushing forward for efficient detection of various analytes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Jukai Zhou,Shuqing Zhang,Xin Song,Ran Wei,Xiang Zhang,Weifeng Zhao,Changsheng Zhao
Recently, in order to increase and improve treatment efficacy of hemoperfusion, research and development of novel adsorbents have been paid much attention. Herein, three-dimensional (3D) graphene oxide (GO) skeleton guided poly(acrylic acid) (PAA) composite hydrogel particles (HPs) used for hemoperfusion were prepared by a core–shell template method. The 3D GO skeletons with millimeter-scale were first constructed with the assistance of templates, dissolvable poly(ether sulfone) hollow particles, which were prepared by a phase inversion technique; then, PAA hydrogels were formed and distributed on the surface of the 3D GO skeleton. The obtained HPs showed a special hierarchical pore microstructure, small size, and uniform spherical shape. Compared with pure PAA hydrogels, the swelling ratios of the HPs were significantly restricted, and the porosities were remarkably increased; the compressive stress and the elongation at break were improved at the same time. The hemocompatibility tests, including protein adsorption, hemolysis ratio, complement and contact activation, platelet adhesion, and clotting time, were carried out, and the results indicated that the HPs showed good hemocompatibility. The adsorption results indicated that the HPs exhibited good removal efficiency toward exogenous and endogenous toxins. In general, the suitable physical and mechanical properties and the excellent hemocompatibility and adsorption capacities made the composite hydrogel particle a promising candidate for novel hemoperfusion adsorbents.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 15
·3361-3372
·
IF 3.286
Zhang Yingyi,Li Liang,Wang Qi,Shen Mei,Han Weili,Yang Xuemei,Chen Lingyun,Ma Ande,Zhou Zhengzheng
Methamphetamine (METH) is one of the most highly addictive illicit drugs abused all over the world. Much evidence indicates that METH abuse leads to major toxicity, medical consequences, and even severe public health consequences. Existing studies usually focus on the pathomechanism of METH-induced toxicity; therefore, data on metabolites and elements correlating with particular toxicity remain scarce. The objective of the present study is to develop appropriate analytical procedures to identify the differential metabolic and elemental biomarkers on METH-induced hepatic injury to rats. The rats were administrated with METH (15 mg/mL/kg, two times per day) via intraperitoneal (i.p.) injections for four consecutive days. The alanine aminotransferase and aspartate aminotransferase activity levels of in the rat serum of the METH group increase significantly compared with those of the control group, suggesting obvious hepatic injury. The results are further confirmed by the histopathological microscopic observation. A total of 18 small molecular metabolites and 19 elements are selected to perform the simultaneous quantification based on the combination of liquid chromatography coupled with tandem mass spectrometry and inductively coupled plasma mass spectrometry. Sample preparation was optimized to cover all the analytes. Both methods are optimized and validated according to developed guidelines such as limits of detection, limits of quantification, linearity, precision, and recovery. All the obtained data are within the satisfactory range. The normalized data were processed according to the partial least squares discrimination analysis (PLS-DA) model. Five differential metabolic and six elemental markers are identified in rat plasma based on the variable importance in projection (VIP) (> 1) and t test results. Overall, the results obtained in this study demonstrate the developed methods are suitable for simultaneous determination of metabolic and elemental markers in the hepatic injury to rats induced by METH.
Graphical abstract
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 12
·2266
·
IF 3.06
Xueying Liu,Xiaoku Ran,Muhammad Riaz,Haixue Kuang,Deqiang Dou,Decheng Cai
The major objective of this study was to investigate the anti-chronic nonbacterial prostatitis (CNP) mechanism ofT. patulaby metabolomics and network pharmacology. The study demonstrated that the flavonoids and polysaccharides ofT. patulacould alleviate prostatitis by improving the level of DHT, reducing the secretion of PSA and TNF-α. Besides, both could enhance Na+/K+-ATPase activity, decrease the O2consumption, CO2production, heat production, energy expenditure of rats and promote respiratory exchange ratio of rats. Up to 28 potential biomarkers and 8 key metabolic pathways related to the treatment of CNP were elucidated by the metabolomics analysis, including phenylalanine metabolism, taurine and hypotaurine metabolism, tryptophan metabolism etc. Network pharmacology prediction also reflected the potential mechanism was associated with tryptophan metabolism and energy pathway. Generally, the potential anti-CNP mechanism of flavonoids and polysaccharides ofT. patulamight be through reducing the expression of inflammation factors, adjusting the level of hormone and regulating the amino acid metabolism, energy metabolism and glucose and lipid metabolism.Keywords:Tagetes patulaL.;chronic nonbacterial prostatitis;metabolomics;energy metabolism;network pharmacology
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Nian Cao,Faqiong Zhao,Baizhao Zeng
In this work, a novel self-enhanced solid state electrochemiluminescence (ECL) platform, based on polyethyleneimine (PEI) capped CdS quantum dots (PEI-CdS QDs) as coreactant and [email protected] 2 @Ru(bpy) 3 2+ doped silica ( [email protected] 2 @RuDS) nanocomposite as ECL emitter, was constructed. The PEI-CdS QDs integrated double coreactant of PEI and CdS QDs, thus significantly promoted the ECL of [email protected] 2 @RuDS. Polyaniline hydrogel was introduced to boost the electron transfer and to improve the immobilization of PEI-CdS/ [email protected] 2 @RuDS. In addition, creatinine imprinted poly(o-aminophenol) film was electrodeposited on the PEI-CdS/ [email protected] 2 @RuDS surface as recognition element. Thus, the obtained sensor possessed high sensitivity, stability, reproducibility and selectivity for creatinine sensing. The change of ECL intensity and logarithm of creatinine concentration showed a good linear relationship in the range of 0.05 nM to 5 μM, and the detection limit was low to 0.02 nM. The sensor was applied to detect creatinine in human serum and urine samples, and the results were in good agreement with those of commercial enzyme-linked immunosorbent assay kit.
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2018
·Vol. 9
·期 1
·1-11
·
IF 12.353
Yang Facui,Tao Fei,Li Chen,Gao Lingxiang,Yang Peng
The design and scalable construction of robust ultrathin protein membranes with tunable separation properties remain a key challenge in chemistry and materials science. Here, we report a macroscopic ultrathin protein membrane with the potential for scaled-up fabrication and excellent separation efficiency. This membrane, which is formed by fast amyloid-like lysozyme aggregation at air/water interface, has a controllable thickness that can be tuned to 30–250 nm and pores with a mean size that can be tailored from 1.8 to 3.2 nm by the protein concentration. This membrane can retain > 3 nm molecules and particles while permitting the transport of small molecules at a rate that is 1~4 orders of magnitude faster than the rate of existing materials. This membrane further exhibits excellent hemodialysis performance, especially for the removal of middle-molecular-weight uremic toxins, which is 5~6 times higher in the clearance per unit area than the typical literature values reported to date.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2018
·
IF 2.88
Ying Shi,Yanjiao Pang,Ning Huang,Chaoqun Sun,Yadi Pan,Yuan Cheng,Yijuan Long,Huzhi Zheng
Dopamine (DA), a catecholamine neurotransmitter , is considered to be an important indicator for mental diseases detection in the clinic. In this study, a novel fluorescent sensing platform consisting of the ficin-H 2 O 2 -tyramine system for determining DA in cerebrospinal fluids (CSF) was established. The proposed method is based on the fact that ficin, a mimetic peroxidase , can catalyze H 2 O 2 decomposition into OH radicals, which can oxidize non-fluorescent tyramine into fluorescent dityramine. When DA was introduced, DA can compete with tyramine for OH and resulting in the oxidation reaction of tyramine inhibited along with the fluorescence intensity of the system decreased, which provides a unique strategy for fluorescence detection of DA. Under optimal conditions, the fluorescence intensity decreased linearly with the DA level over a wide concentration range from 0.05 to 12.0 μM (R 2 = 0.995) with a detection limit of 46 nM (3σ/k). More importantly, the proposed sensing approach exhibits high sensitivity, good selectivity and has been successfully applied to DA sensing in complex biological samples, which made it hold great potential for DA determination in chemical and biological analytical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULOSE
·
2018
·Vol. 25
·期 12
·6947-6961
·
IF 3.809
Zhang Lianwei,Wang Ruijia,Liu Rui,Du Xiaolin,Meng Ranju,Liu Lin,Yao Juming
Abstract In this work, a novel colorimetric sensor was prepared via a cellulose-polyethylenimine-based Schiff base (cellulose-Schiff base) for capturing and detecting copper ions (Cu 2+ ) in aquatic environments and life systems. The cellulose-Schiff base displayed continuous color changes from inherent pale yellow to deep green due to the formation of a cellulose-Schiff base-Cu 2+ charge transfer complex within 30 s, indicating excellent “naked-eye” detection capability for Cu 2+ ions in aqueous solution. The limit of detection (LOD) was 1.054 ppm, lower than the U.S. EPA guidelines for drinking water of 1.3 ppm. Moreover, the cellulose-Schiff base colorimetric sensor was successfully used to capture and detect exogenous Cu 2+ ions in biofluids (simulated urine and bovine serum). The proposed sensing mechanism of the cellulose-Schiff base for Cu 2+ ions is that N atoms (C=N and N–H) in the sensor capture and concentrate Cu 2+ ions to form sensor-Cu 2+ complexes, then intramolecular charge transfer occurs as a consequence. In addition, test strips based on cellulose-Schiff base were developed, and provide a convenient, cheap, sensitive, and reliable monitoring platform for on-site semi-quantitative analysis of Cu 2+ ions in real samples. Graphical abstract
✓ 已验证
产品关联
DOI
高影响因子
Biomedical Optics Express
·
2018
·Vol. 9
·期 10
·4988-4997
·
IF 3.482
Yudong Lu,Changji Wu,Ruiyun You,Yang Wu,Huiying Shen,Lanjin Zhu,Shangyuan Feng
Superhydrophobic silver films were fabricated by silver-mirror reaction and surface functionalization with thiol. The thiol-functionalization significantly improved the hydrophobic property of the Ag films (AFS), and their contact angle values slightly increased with the extension of a thiol alkyl chain, reaching about 160°. The surface-enhanced Raman scattering (SERS) detection capacity of these films were investigated, and AFS-Dodec showed the best substrate for R6G molecule detection with the concentration limit of 10−11 M. AFS functionalized with dodecanethiol (AFS-Dodec) was applied for the SERS detection of uric acid and creatinine, it exhibited good linear dependence relationship between the Raman intensity and analyte concentration in the concentration range of 5~1000 μM.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2015
·
IF 4.074
Weiqing Kong,Qingqing Dai,Junli Ren,Nianfang Ma
A new xylan ester (xylan 3,5-dinitrobenzoate) as a creatinine adsorbent was prepared by the homogeneous acylation of xylan with 3,5-dinitrobenzoyl chloride in 1-butul-3-methylimidazolium chloride ionic liquid. The influences of reaction conditions on the degree of substitution values of xylan esters were discussed. Results indicated that xylan esters with the degree of substitution range from 1.34 to 1.77 were obtained under the given conditions. The FTIR and 13 C NMR spectroscopies provided the evidence of grafting 3,5-dinitrobenzoyl groups onto the backbone of xylan. Moreover, the adsorption properties of the xylan ester for creatinine were also investigated. Isotherm studies showed that the sorption capacities for creatinine were 2.45, 2.08 and 1.86 mg/g for 23, 30 and 37 °C, respectively. Thermodynamic studies performed indicated the sorption process mainly was controlled by the chemical adsorption. Therefore, xylan 3,5-dinitrobenzoate displayed the promising application in the treatment of chronic renal failure by the creatinine adsorption as the new oral adsorbent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2014
·
IF 4.908
Li-Jing Zhu,Fu Liu,Xue-Min Yu,Ai-Lin Gao,Li-Xin Xue
Poly(lactic acid) (PLA) hemodiafiltration membranes were grafted by zwitterionic polymer to improve the fouling resistance and hemocompatibility. The surface zwitterionization was accomplished by dopamine inspired bromoalkyl initiator anchoring and subsequent atom transfer radical polymerization (ATRP) of zwitterionic poly(sulfobetaine methacrylate) (PSBMA). Attenuated total reflectance Fourier transform infrared spectra (ATR-FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) were applied to analyze the surface chemistry and morphology evolution of the fabricated membranes, which confirmed that zwitterionic PSBMA was successfully grafted on PLA membrane. The results of water contact angle and zeta potentials demonstrated that the hydrophilicity and negativity of the modified PLA membranes were highly improved, which effectively alleviated the surface adsorption of bovine serum albumin (BSA) and palates. Moreover, the modified PLA membrane exhibited good dialysis performances. When the polymerization time of PSBMA was 1 h, the water flux was 184 L m −2 h −1 , while BSA retention reached up to 90%, and the clearance of urea and creatinine was maintained at 66% and 60%, respectively. The present work provides a convenient strategy to improve the hydrophilicity, anti-fouling and hemocompatibility of PLA membranes for their biomedical and blood-contacting applications such as hemodialysis.
✓ 已验证
产品关联
DOI
高影响因子
SYNTHETIC METALS
·
2014
·
IF 2.222
Fang Liao,Siwei Yang,Xiubing Li,Lijun Yang,Zhihui Xie,Chenshu Hu,Ling He,Xinyuan Kang,Xun Song,Tongyan Ren
Poly(o-phenylenediamine) and benzeneselenol copolymer fluorescent nanorods were synthesized via a simple hydrothermal treatment and a novel, simple and ultra-sensitive oxidation–reduction fluorescent switch system was prepared based on these copolymer fluorescent nanorods. This switch system can be triggered by redox procedure. When this copolymer is oxidized by H 2 O 2 , the switch is “off”. The switch system is “on” when glutathione (GSH) is added. This switch system has good stability, excellent selectivity and specificity. What is more, the low detective limit indicates (detection limit: 10 −12 M) this switch system can be used as an ultra-sensitive fluorescent probe for H 2 O 2 or GSH. For the mechanism of switch processes, XPS results show the change of binding mode of Se in this copolymer is the main cause.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）