MICROCHIMICA ACTA
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2022
·Vol. 189
·期 12
·1-10
·
IF 6.408
Sun Min,Wang Xiuqin,Ding Yali,Feng Juanjuan
In order to improve the selectivity and stability of melamine–formaldehyde (MF) aerogel, it was composited with TiO 2 aerogel. A TiO 2 -MF hybrid aerogel was in situ prepared on the surface of carbon fibers for in-tube solid-phase microextraction (SPME). The extraction performance of TiO 2 -MF aerogel was regulated by changing the ratio of TiO 2 sol and MF sol during the material preparation. Coupled with high-performance liquid chromatography-diode array detection (HPLC–DAD), the extraction tube filled by TiO 2 -MF aerogel-coated carbon fibers was evaluated with several types of environmental pollutants including polycyclic aromatic hydrocarbons (PAHs), estrogens, and ultraviolet filters. Because of favourable extraction performance of PAHs they were selected as model analytes, and some important influence factors were optimized for satisfactory sensitivity. The detection limits were in the range 0.05–0.10 μg L −1 , owing to high enrichment factors (653–1007). The online in-tube SPME-HPLC–DAD method was verified for the determination of trace PAHs in environmental water samples, and acceptable recovery (70–118%) was achieved. The analytical methods also displayed some advantages in comparison with other reports. Moreover, the extraction tube exhibited satisfactory chemical stability. Graphical abstract
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Acta Biomaterialia
·
2023
·
IF 9.7
Chen Zhong,Haoran Zhu,Yinying Sheng,Jin Wo,Deqiang You,Guodong Sun,Zhentao Yu,Wei Li,Xiaojian Wang
Zinc alloys have demonstrated considerable potentials as implant materials for biodegradable vascular and orthopedic applications. However, the high initial release of Zn 2+ can trigger intense immune responses that impede tissue healing. To address this challenge and enhance the osteogenic capacity of zinc alloys, the surface of Zn1Mg was subjected to CO 2 plasma modification (Zn1Mg-PP) followed by grafting with choline phosphate chitosan (Zn1Mg-PP-PCCs). This study aims to investigate the in vitro and in vivo biocompatibility of the surface-modified Zn1Mg. The effect of the surface modification on the inflammatory response and osteogenic repair process was investigated. Compared with unmodified Zn1Mg, the degradation rate of Zn1Mg-PP-PCCs was significantly decreased, avoiding the cytotoxicity triggered by the release of large amounts of Zn 2+ . Moreover, PCCs significantly enhanced the cell-material adhesion, promoted the proliferation of osteoblasts (MC3T3-E1) and upregulated the expression of key osteogenic factors in vitro . Notably, the in vivo experiments revealed that the surface modification of Zn1Mg suppressed inhibited the expression of inflammatory cytokines, promoting the secretion of anti-inflammatory factors, thereby reducing inflammation and promoting bone tissue repair. Furthermore, histological analysis of tissue sections exhibited strong integration between the material and the bone, along with well-defined new bone formation and reduced osteoclast aggregation on the surface. This was attributed to the improved immune microenvironment by PCCs, which promoted osteogenic differentiation of osteoblasts. These findings highlight that the preparation of PCCs coatings on zinc alloy surfaces effectively inhibited ion release and modulated the immune environment to promote bone tissue repair. Statement of significance Surface modification of biodegradable Zn alloys facilitates the suppression of intense immune responses caused by excessive ion release concentrations from implants. We modified the surface of Zn1Mg with choline phosphate chitosan (PCCs) and investigated the effects of surface modification on the inflammatory response and osteogenic repair process. In vitro results showed that the PCCs coating effectively reduced the degradation rate of Zn1Mg to avoid cytotoxicity caused by high Zn 2+ concentration, favoring the proliferation of osteoblasts. In addition, in vivo results indicated that Zn1Mg-PP-PCCs attenuated inflammation to promote bone repair by modulating the release of inflammation-related factors. The surface-modified Zn1Mg implants demonstrated strong osseointegration, indicating that the PCCs coating effectively modulated the immune microenvironment and promoted bone healing.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiumin Yang,Junmin Wang,Guifen Chang,Cuihong Sun,Qiuhua Wu,Zhi Wang
Analysis of organochlorine pesticides (OCPs) residues in milk faces a significant challenge. Herein, a sea urchin structured covalent organic framework bearing boric acid groups named COF-B(OH) 2 was synthesized and applied as a coating material for solid-phase microextraction (SPME) of the OCPs in cattle’s milk. Its performance was superior to that of three commonly used commercial SPME fibers, which could be due to the coexistence of hydrogen bonding , halogen bonding, π-stacking and electrostatic interactions. Besides, the fiber coating displayed good stability and reusability. After optimization, a COF-B(OH) 2 based SPME coupled with gas chromatography-electron capture detection was established for the sensitive detection of the OCPs from milk samples. The limits of detection (S/N = 3) were between 0.04 and 1.00 μg kg −1 . Satisfactory accuracy was achieved with the method recoveries in the range of 87.5 % to 112.5 %. These results manifest the feasibility of the COF-B(OH) 2 coated fiber for the enrichment of the trace OCPs from milk samples.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Zhaowen Liu,Hongcai Wang,Yanan Bu,Ting Wu,Xiuqiong Chen,Huiqiong Yan,Qiang Lin
The application of hydrophobic β-carotene in the food industry are limited due to its susceptibility to light, high temperature, pH value, and other factors, leading to poor stability and low bioavailability. To address this problem, we adopt a more green and environmentally friendly reducing agent, 2-methylpyridine borane complex (pic-BH 3 ), instead of traditional sodium borohydride, to achieve the simple green and efficient synthesis of amphiphilic oxidized sodium alginate grafted oleoamine derivatives (OSAOLA) through the reduction amination reaction of Schiff base. The resultant OSAOLA with the degree of substitution (DS) of 7.2 %, 23.6 %, and 38.8 % were synthesized, and their CMC values ranged from 0.0095 to 0.062 mg/mL, indicating excellent self-assembly capability in aqueous solution. Meanwhile, OSAOLA showed no obvious cytotoxicity to RAW 264.7 cells, thus revealing good biocompatibility. Furthermore, β-carotene, as the hydrophobic active ingredients in foods was successfully encapsulated in the OSAOLA micelles by ultrasonic-dialysis method. The prepared drug-loaded OSAOLA micelles could maintain good stability when stored at room temperature for 7 d. Additionally, they were able to continuously release β-carotene and exert long-term effects in pH 7.4 PBS at 37 °C, effectively improving the bioavailability of β-carotene, which exhibited tremendous application potential in functional food and biomedical fields.
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JOURNAL OF MOLECULAR LIQUIDS
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2023
·
IF 6
Wenshuai Qi,Quanhua Deng,Na Du,Wanguo Hou
Surfactant-free microemulsions (SFMEs) refer to the microemulsions formed with two immiscible fluids (generally called the oil and water phases) in the presence of an amphi -solvent rather than the traditional surfactants. The understanding of SFMEs is still limited now. In the current work, the phase behavior of the ternary mixture of n -butanol (oil phase), ethanol ( amphi -solvent), and water was investigated. The ternary phase diagram shows a single-phase homogeneous region and a multiphase region. The mesoscale structuring was observed within the macroscopically homogeneous single-phase zone near the phase boundary using dynamic light scattering (DLS) and small-wide angle X-ray scattering (SWAXS) techniques. That is, microemulsions are formed in the ternary mixture without the traditional surfactants. In addition, the electrical conductivity and micropolarity of the single-phase mixtures were determined, suggesting the existence of three types of microstructures, i.e. , oil-in-water (O/W), bicontinuous (BC), and water-in-oil (W/O), for the SFMEs. The subregions corresponding to the three microstructures were roughly identified in the ternary phase diagram. n -Butanol is one of the extremely weak hydrophobic substances, and the detection of microemulsions in the mixture indicates that the formation of SFMEs is a universal phenomenon. This study provides a better understanding of the phase behavior of ternary liquid mixtures.
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Processes
·
2023
·Vol. 11
·期 7
·2199
·
IF 3.5
Liang Shen,Yifang Liu,Jiabao Gong,Erle Qiao
Soil is the main aggregation site of polycyclic aromatic hydrocarbons and an important pathway of migration to other media. In this paper, the adsorption behavior of pyrene and seven different types of surfactants on kaolinite surfaces was studied by molecular dynamics simulation and desorption testing. The molecular dynamics simulation results showed that pyrene was more easily adsorbed on the 001 (-) side of kaolinite. SDBS, SDS, TW80, and TX-100 had strong interactions with pyrene, encapsulating pyrene molecules in aggregates. However, when the concentration of surfactant was too high, the desorption of pyrene molecules on a kaolinite surface will be inhibited. The desorption of pyrene molecules will be inhibited in the presence of BS-12, TW80, and TX-100, while the desorption process can be promoted by using CTAC, DDBAC, SDBS, and SDS as soil remediation agents. The removal rate of pyrene gradually increased with the increase of SDS dosage, while for SDBS, the removal rate showed a trend of first increasing and then decreasing. When the concentration of SDS was 0.014 mol/L, the elution rate of pyrene reached 72.86%. The molecular dynamics simulation results were similar to the desorption test results, verifying the reliability of molecular dynamics simulation. The research results provide theoretical support for the selection of surfactants in the remediation process of pyrene-contaminated soil.Keywords:soil remediation;polycyclic aromatic hydrocarbons;surfactants;molecular simulation
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Advanced Healthcare Materials
·
2023
·
IF 10
Xinmiao Lan,Jie Zhong,Regina Huang,Yuhan Liu,Xiaowei Ma,Xuan Li,Dan Zhao,Guangchao Qing,Yuxuan Zhang,Lu Liu,Jinjin Wang,Xu Ma,Ting Luo,Weisheng Guo,Yuji Wang,Li-Li Li,Yu-Xiong Su,Xing-Jie Liang
Antimicrobial peptides (AMPs) are a developing class of natural and synthetic oligopeptides with host defense mechanisms against a broad spectrum of microorganisms, including viruses, bacteria, and fungi. With in-depth research on the structural conformations of AMPs, synthesis or modification of peptides has shown great potential in effectively obtaining new therapeutic agents with improved physicochemical and biological properties. Notably, AMPs with self-assembled properties have gradually become a hot research topic for various materials and biomedical applications. Compared to monomeric peptides, these peptides can exist in diverse forms (e.g., nanoparticles, nanorods, and nanofibers) and possess several advantages, such as high stability, good biocompatibility, and potent biological functions, after forming aggregates under specific conditions. In particular, the stability and antibacterial property of these AMPs can be modulated by rationally regulating the peptide sequences to promote self-assembly, leading to the reconstruction of molecular structure and spatial orientation while introducing some peptide fragments into the scaffolds. In this work, we have developed four self-assembled AMPs, and the relationship between their chemical structures and antibacterial activity were explored extensively through different experiments. Importantly, the evaluation of antibacterial performance in both in vitro and in vivo studies has provided a general guide for using self-assembled AMPs in subsequent treatments for combating bacterial infections. This article is protected by copyright. All rights reserved
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SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Haohao Li,Yanrong Li,Fengshan Liu,Yang Xu,Huanrong Li
Microplastics (MPs) pollution has become a vital issue of worldwide concern owing to its associated hazards and the ubiquitous accumulation of MPs particles, which is correlated to their small sizes. Rapid identification and quantification of MPs represents an ongoing challenge in the field of environmental research. In this work, we prepared a fluorescence nanoprobe for identifying polyethylene (PE) MPs and developed a readily available method for the fast quantification of PE MPs in fluorescence mode. A kind of sulfur-doped carbon nanoparticles (S-CNPs) was prepared using nitrated pyrene and dithiosalicylic acid as precursors via a solvothermal process. S-CNPs showed no fluorescence emission in the solid state, whereas visible fluorescence was observed by labeling PE MPs. The surface environment-dependent emission of S-CNPs is an important factor in explaining the different emissions of different types of MPs, such as PE, polyvinyl chloride (PVC), and polystyrene (PS) for MPs identification. Notably, the distinction between PE and polypropylene (PP) was achieved using these S-CNPs according to their different staining efficiencies. Owing to its fast staining ability, a paper-based method for PE MPs quantification in water samples was developed using a simple vacuum-assisted suction filtration method and filter paper soaked with S-CNPs. Utilized as a readily available identification and quantification platform, these S-CNPs have great potential and could pave the way for MPs monitoring.
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Acta Biomaterialia
·
2023
·
IF 9.7
Xinshu Zou,Shuang Cai,Tingting Wang,Sidi Zheng,Xilong Cui,Jingyou Hao,Xueying Chen,Yanyan Liu,Zhiyun Zhang,Yanhua Li
Intracellular MRSA is extremely difficult to eradicate by traditional antibiotics, leading to infection dissemination and drug resistance. A general lack of facile and long-term strategies to effectively eliminate intracellular MRSA. In this study, glabridin (GLA)-loaded pH-responsive nanoparticles (NPs) were constructed using cinnamaldehyde (CA)-dextran conjugates as carriers. These NPs targeted infected macrophages/MRSA via dextran mediation and effectively accumulated at the MRSA infection site. The NPs were then destabilized in response to the low pH of the lysosomes, which triggered the release of CA and GLA. The released CA downregulated the expression of cytotoxic pore-forming toxins, thereby decreasing the damage of macrophage and risk of the intracellular bacterial dissemination. Meanwhile, GLA could rapidly kill intracellularly entrapped MRSA with a low possibility of developing resistance. Using a specific combination of the natural antibacterial agents CA and GLA, NPs effectively eradicated intracellular MRSA with low toxicity to normal tissues in a MRSA-induced peritonitis model. This strategy presents a potential alternative for enhancing intracellular MRSA therapy, particularly for repeated and long-term clinical applications. Statement of significance Intracellular MRSA infections are a growing threat to public health, and there is a general lack of a facile strategy for efficiently eliminating intracellular MRSA while reducing the ever-increasing drug resistance. In this study, pH-responsive and macrophage/MRSA-targeting nanoparticles were prepared by conjugating the phytochemical cinnamaldehyde to dextran to encapsulate the natural antibacterial agent glabridin. Using a combination of traditional Chinese medicine, the NPs significantly increased drug accumulation in MRSA and showed superior intracellular and extracellular bactericidal activity. Importantly, the NPs can inhibit potential intracellular bacteria dissemination and reduce the development of drug resistance, thus allowing for repeated treatment. Natural antibacterial agent-based drug delivery systems are an attractive alternative for facilitating the clinical treatment of intracellular MRSA infections.
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WATER AIR AND SOIL POLLUTION
·
2023
·Vol. 234
·期 7
·1-13
·
IF 2.9
Zhou Danli,Zhang Dong,Zhang Ming,Lü Ting,Huang Jingang,Zhao Hongting,Lin Jun
To clarify the sorption mechanism and removal efficiency of PAHs by waste biomass-based magnetic sorbents, the sorption performance of magnetic Myriophyllum aquaticum biomass (MMaB) for PAHs was studied. The structural characteristics and magnetic properties of MMaB were measured by FTIR, SEM–EDS, XRD and VSM. Batch experiments were carried out to evaluate the effects of contact time, initial concentration and properties of PAHs, pH, ionic strength and temperature on PAH sorption. The results showed that the sorption of seven PAHs achieved equilibrium at approximately 4–6 h, which fit well to the pseudo-second-order kinetic model. The isotherms followed the Freundlich model and linear model well, indicating that the sorption process was controlled by chemisorption. The distribution parameter ( K d ) values were 91.3, 563.7, 1010.5, 2917.8, 2706.6, 9046.8 and 15969.0 L·kg −1 for naphthalene, acenaphthene, fluorene, phenanthrene, anthracene, pyrene, and fluoranthene on MMaB, respectively, following the order of their hydrophobicity (e.g., K ow ). A positive correlation was obtained: log K d = 0.8091log K ow + 1.7034. Water chemical properties such as pH and ionic strength had no obvious effect on PAH removal. The spontaneity, feasibility and exothermic properties of PAH removal by MMaB were explained from thermodynamic point of view. The as-prepared MMaB sorbent underwent four consecutive sorption cycles and had good cycling performance. These studies indicate that MMaB has great potential as an easily-prepared, easy-to-operate and efficient biosorbent to remove PAHs from wastewater.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Furong Jin,Xinyue Yin,Yang Wan,Jiukai Zhang,Jindi Wang,Xiangbo Fu,Tianxin Fu,Buwei Liu,Yongshi Chen,Bo Tian,Zhibiao Feng
It is important to detect the presence of biogenic amines (BAs) as indicators of food freshness. The purpose of this study was to develop a novel ultrasonic–microwave synergistic supramolecular solvent liquid–liquid microextraction based on solidification of floating organic droplet (UMS-SUPRAS-SFO-LLME) combined with high-performance liquid chromatography for the determination of BAs. The physical properties and microstructure of SUPRAS based on 1-dodecanol and tetrahydrofuran were studied, and the extraction conditions such as the SUPRAS volume, the UMS process, and the centrifugal conditions were optimized. The results for the extraction kinetics and thermodynamics showed that UMS-SUPRAS-SFO-LLME is a spontaneous, endothermic diffusion process. The linear ranges of this method are 0.1–2.0 × 10 5 ng·mL −1 ( R 2 > 0.994), the limits of detection are 4.0 × 10 -3 -6.0 × 10 -2 ng·mL −1 , and the recoveries were 96.28–103.15%. Compared with existing analysis methods, UMS-SUPRAS-SFO-LLME is a sensitive, green and economical sample pretreatment method for analyzing the enrichment of BAs in beer and fish.
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OPTICS AND LASER TECHNOLOGY
·
2023
·
IF 5
Weifan Pan,Qian Zhang,Anyi Qian,Zhui Yang,Yanbing Zhang,Caiqin Han,Peitong Duan
A kind of gold nanoparticles modified silver nanorods array (AuNPs/AgNRs) composite structure substrate with good Surfacing-enhanced Raman scattering (SERS) property was prepared by oblique angle deposition (OAD) method and chemical synthesis. According to the simulation results, the electric field enhancement effect of AuNPs/AgNRs composite substrate was the best when the inclination angle of silver rod was 72°, the length of silver rod was 1000 nm and the diameter of gold nanoparticles was 60 nm. CV and R6G were used as probe molecules to explore SERS activity of this substrate. The results exhibited that the detection limits of CV and R6G were both 10 −7 M, and the relative standard deviations (RSDs) were all less than 10.28 %. With simple preparation and high SERS activity, AuNPs/AgNRs substrate has good application prospect and research value when applied in the determination of polycyclic aromatic hydrocarbons represented by organic compound pyrene.
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