Catalysis Science & Technology
·
2018
·Vol. 9
·期 2
·477-482
·
IF 5.365
Caixia Cui,Yunming Fang,Biqiang Chen,Tianwei Tan
The construction and catalytic application of multi-enzymatic systems have attracted much research attention. Herein, we demonstrate an effective approach for the construction of a glucose oxidase (GOX) and catalase (CAT) matrix via the supramolecular recognition of β-cyclodextrin (grafted to GOX) and adamantane (grafted to CAT). This forms a self-assembling multi-enzyme system (SAME) spontaneously after simple mixing. In the SAME, GOX activity and kcat/Km were 3.7 and 7 times greater than in the GOX/CAT mixture. Kinetic analysis indicated that the SAME improved catalysis because hydrogen peroxide was rapidly consumed by the adjacent CAT. Furthermore, the mixture and SAME was used to synthesize gluconic acid from glucose, the GOX/CAT SAME exhibited higher reaction rates than did the GOX/CAT mixtures at the same glucose concentrations; these differences were particularly noticeable at higher substrate glucose concentrations. These results suggest that SAMEs constructed by the method described herein might increase cascade reaction rates and be more suitable for high glucose concentrations.
研究领域:
生物化学
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酶工程
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DOI
高影响因子
Nanoscale
·
2017
·Vol. 9
·期 44
·17561-17570
·
IF 7.367
Xiao Liu,Wei Qi,Yuefei Wang,Rongxin Su,Zhimin He
Metal–organic frameworks (MOFs) have drawn extensive research interest as candidates for enzyme immobilization owing to their tunable porosity, high surface area, and excellent chemical/thermal stability. Herein, we report a facile and universal strategy for enzyme immobilization using highly stable hierarchically porous metal–organic frameworks (HP-MOFs). The HP-MOFs were stable over a wide pH range (pH = 2–11 for HP-DUT-5) and met the catalysis conditions of most enzymes. The as-prepared hierarchical micro/mesoporous MOFs with mesoporous defects showed a superior adsorption capacity towards enzymes. The maximum adsorption capacity of HP-DUT-5 for glucose oxidase (GOx) and uricase was 208 mg g−1 and 225 mg g−1, respectively. Furthermore, we constructed two multi-enzyme biosensors for glucose and uric acid (UA) by immobilizing GOx and uricase with horseradish peroxidase (HRP) on HP-DUT-5, respectively. These sensors were efficiently applied in the colorimetric detection of glucose and UA and showed good sensitivity, selectivity, and recyclability.
研究领域:
生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zhiwen Gan,Tian Zhang,Xuanxuan An,Qin Tan,Shujun Zhen,Yongmei Hu,Xiaoli Hu
Many nanozymes have been synthesized, though, it is still necessary to rationally synthesize multifunctional nanozymes for diverse applications. Herein, we fabricate three fluorescent nanozymes with peroxidase- and laccase-like activities under moderate conditions. Three nanozymes, NH 2 BDC/CuFe/ATP (NPs-1), NH 2 BDC/CuFe/ADP (NPs-2), NH 2 BDC/CuFe/AMP (NPs-3), are constructed based on three adenosine phosphates coordinated transition metal ions (Cu 2+ and Fe 3+ ) and adsorbing fluorescent amino terephthalic acid (NH 2 -BDC). They exhibit different laccase-like activity, which may result from the difference in steric configurations of nanozymes. Among them, NPs-1 possesses high peroxidase-mimicking activity and stability. Interestingly, o-phenylenediamine (OPD) is oxidized to 2,3-diaminophenazine (DAP) by ·OH which NPs-1 catalyzes H 2 O 2 to produce. The fluorescence of NPs-1 ( λ em = 445 nm) is effectively reduced based on the inner filter effect between DAP and NPs-1, which recovers after adding AA. Based on stable fluorescence and high peroxidase-like activity of NPs-1, a system for determining H 2 O 2 and AA is established in linear ranges of 2.0–200.0 and 4.0–300.0 μmol L −1 with limits of detection of 1.82 and 2.92 μmol L −1 . The fluorescent probe shows a good performance in detecting AA in real samples. Furthermore, NPs-1 is applied in smartphone-based detection of AA by measuring its intrinsic fluorescence. This work provides a facile strategy for the design of multi-functional nanozymes.
研究领域:
生物化学
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分析化学
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纳米技术
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DOI
高影响因子
Nanomaterials
·
2021
·Vol. 11
·期 9
·2171
·
IF 5.076
Le Wang,Pengxue Sun,Yiyu Yang,Hanzhen Qiao,Hailong Tian,Dapeng Wu,Shuoye Yang,Qipeng Yuan,Jinshui Wang
The field of enzyme cascades in limited microscale or nanoscale environments has undergone a quick growth and attracted increasing interests in the field of rapid development of systems chemistry. In this study, alcohol dehydrogenase (ADH), lactate dehydrogenase (LDH), and mesoporous silica nanoparticles (MSN) immobilized nicotinamide adenine dinucleotide (NAD+) were successfully immobilized on the zeolitic imidazolate frameworks (ZIFs). This immobilized product was named ZIF@ADH/NAD-MSN/LDH, and the effect of the multi-enzyme cascade was studied by measuring the catalytic synthesis of lactic acid. The loading efficiency of the enzyme in the in-situ co-immobilization method reached 92.65%. The synthesis rate of lactic acid was increased to 70.10%, which was about 2.82 times that of the free enzyme under the optimal conditions (40 °C, pH = 8). Additionally, ZIF@ADH/NAD-MSN/LDH had experimental stability (71.67% relative activity after four experiments) and storage stability (93.45% relative activity after three weeks of storage at 4 °C; 76.89% relative activity after incubation in acetonitrile-aqueous solution for 1 h; 27.42% relative activity after incubation in 15% N, N-Dimethylformamide (DMF) solution for 1 h). In summary, in this paper, the cyclic regeneration of coenzymes was achieved, and the reaction efficiency of the multi-enzyme biocatalytic cascade was improved due to the reduction of substrate diffusion.Keywords:confinement effect;multi-enzyme catalytic cascade;in-situ synthesis;coenzyme recycling;co-immobilization enzyme
研究领域:
生物化学
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纳米生物学
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系统化学
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产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2018
·
IF 3.974
Shuyan Zhao,Tiankun Liang,Tao Zhou,Dongqi Li,Bohui Wang,Jingjing Zhan,Lifen Liu
Perfluorooctane sulfonamide (FOSA) is an important perfluorooctane sulfonate (PFOS) precursor used for commercial applications. In order to investigate the transformation and responses of selected antioxidant and degradation enzymes of FOSA in the plants, in vivo exposure of soybean ( Glycine max L. Merrill) and pumpkin ( Cucurbita maxima L.) were conducted in the solution-plant microcosms. FOSA was readily taken up by soybean and pumpkin roots and translocated to shoots, and metabolized to PFOS, perfluorohexane sulfonate (PFHxS) and perfluorobutane sulfonate (PFBS). Although morphological and biomass effects were not visible, significant changes in oxidative stress response were observed except for thiobarbituric acid reactive substances (TBARS). Superoxide dismutase (SOD) and peroxidase (POD) activities were significantly increased by 19.2–30.8% and 19.2–20.7% in soybean (8–12 d) respectively, and increased by 39.2–92.8% and 21.1–37.6% in pumpkin (3–12 d) respectively, suggesting an activation of the antioxidant defense system in the plants exposed to FOSA. Glutathione-S-transferase (GST) activities were decreased in soybean (2–12 d) with 9.0–36.1% inhibition and increased in pumpkin (3–12 d) with 22.5–47.3% activation respectively; cytochrome P450 (CYP450) activities were increased markedly in soybean and pumpkin with 13.2–53.6% and 26.7–50.2% activation respectively, giving indirect evidences on the involvement of CYP450 and GST in degradation of FOSA in plants.
研究领域:
环境科学
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植物生理学
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毒理学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiang Zhou,Jiahao Xu,Jiayao Sun,Hao Chen,Dechun Huang,Wei Chen,Hongliang Qian
1,3-Dioleoyl-2-palmitoylglycerol (OPO) is crucial for infant nutrition; however, conventional immobilized lipase requires high-purity enzymes, which increases costs and limits industrial scalability. Herein, Rhizomucor miehei lipase (RML) was immobilized on surface-modified magnetic nanoparticles using cross-linked enzyme aggregates (CLEAs) technology to produce Fe 3 O 4 @SiO 2 @TPOAC@RML CLEAs. This approach combines the separation and immobilization of enzymes, allowing for the use of lower-purity lipase, which enhances its suitability for industrial-scale processes. The optimized Fe 3 O 4 @SiO 2 @TPOAC@RML CLEAs exhibited excellent thermal, pH, and storage stability. The performance of Fe 3 O 4 @SiO 2 @TPOAC@RML CLEAs was evaluated by catalyzing the synthesis of OPO through the enzymatic hydrolysis of glycerol tripalmitate (PPP) and oleic acid (OA). Under optimal conditions, the OPO content in products reached 63.33 ± 0.30%, highlighting the high efficiency of the enzymatic process. After eight reuse cycles, the Fe 3 O 4 @SiO 2 @TPOAC@RML CLEAs retained around 75% of their relative activity. Therefore, this strategy of lipase immobilization using CLEA technology, coupled with magnetic nanoparticles, offers a promising approach for OPO synthesis.
研究领域:
生物催化
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纳米技术
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酶固定化
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食品化学
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工业生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2017
·Vol. 124
·期 2
·469-479
·
IF 2.099
T. Cheng,X. Xu,W. Zhang,L. Chen,T. Liu
Aims To establish a proper protoplast-preparation route from enriched motile flagellates and nonmotile resting cells of Haematococcus pluvialis . Methods and Results Through cultivations in two mixotrophic media, enriched Haematococcus flagellates and resting cells were respectively produced and applied in enzymatic protoplast preparations. Great differences of enzymatic sensitivity and osmotic-lability were identified between them. Flagellates showed the same osmotic-lability as protoplasts and the extracellular matrix-removing rate was applied for an evaluation of protoplast-releasing. During the treatment of flagellates, an addition of more than 0·2 mmol l −1 Ca 2+ was found to be essential for maintenance of high cellular viability. More than 80% cellular viability and a 90% protoplast-releasing rate were obtained simultaneously after 2–3 h treatment of 0·06% proteinase K in 0·05 mol l −1 Tris-HCl (pH7·8) buffer with 0·2 mmol l −1 CaCl 2 and 0·2 mol l −1 sorbitol/mannitol. For resting cells, a treatment of both 0·12% proteinase K and a combination of 2% cellulase + 1% snailase could function similarly in order to degrade the cellulosic cell wall, while the protoplast yield was limited to about 40%, due to the existence of an undegradable secondary wall in the mature resting cell. Conclusion Proteinase K was efficient for protoplast-releasing from either flagellates or resting cells. Due to the great difference of enzymatic sensitivity and osmotic-lability between flagellates and resting cells, it was necessary to select a different enzymatic treating process based upon the main cell type in the culture. A better protoplast-preparing efficiency was obtained from Haematococcus cells when flagellates were in the majority. Significance and Impact of the Study The protoplast preparation of H. pluvialis was firstly established based on two main cell types of H. pluvialis , motile flagellates and nonmotile resting cells. Increase of flagellate stability and viable protoplast-preparing efficiency through addition of Ca 2+ in enzymatic solution was firstly reported.
研究领域:
微生物学
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生物技术
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植物科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Xin Zheng,Qiulan Tong,Lei Ma,Yunfei Tan,Danni Liu,Yixuan Wu,Xiaomin Ma,Xudong Li,Zeng Yi
Excessive alcohol consumption and prolonged administration of non-steroidal anti-inflammatory drugs (NSAIDs) lead to gastritis which affects millions globally, yet safe and effective treatment options remain limited. While a range of nanozymes have been employed in the oral treatment of inflammatory bowel disease (IBD), the research directed toward gastritis therapy remains unexplored. The challenges of rapid gastric emptying, harsh gastric acid erosion, and the non-edible ingredient constitution hinder the practical use of nanozymes in this context. Herein, three types of sub-10 nm ultrasmall iron-polyphenol nanozymes (UIPNs) were synthesized by using fully edible natural active polyphenols, iron ions and PVP. UIPNs rapidly penetrated the gastric mucosa, resided in the stomach for over 12 h, and remained stable in the harsh acidic environment. They exhibited excellent enzyme-like activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD). Compared to omeprazole, all three types of UIPNs showed superior activity, with Fis UIPNs demonstrating the best performance in preventing and protecting against alcohol-induced gastric mucosal injury in mice. The fully food-sourced components, constructed into ultrasmall nanozymes via non-covalent interactions, ensure long-term safety and hold promise for playing significant roles in various gastrointestinal diseases.
研究领域:
胃肠病学
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纳米技术
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药学
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材料科学
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产品关联
DOI
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2015
·Vol. 171
·期 1
·116-123
·
IF 1.748
Zhang Qiuyue,Cao Zheng,Sun Xudong,Zuang Cuicui,Huang Wanyue,Li Yanfei
Aluminum (Al) is the most abundant metal in the earth’s crust. Al accumulates in erythrocyte and causes toxicity on erythrocyte membrane. The dysfunction of erythrocyte membrane is a potential risk to hypertension. The high Al content in plasma was associated with hypertension. To investigate the effect of AlCl 3 on blood pressure and the function of erythrocyte membrane, the rats were intragastrically exposed to 0, 64(1/20 LD 50 ), 128(1/10 LD 50 ), and 256(1/5 LD 50 ) mg/kg body weight AlCl 3 in double distilled water for 120 days, respectively. Then, we determined the systolic and mean arterial blood pressures of rats, the osmotic fragility, the percentage of membrane proteins, the activities of Na + /K + -ATPase, Mg 2+ -ATPase, Ca 2+ -ATPase, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-pX), and malondialdehyde (MDA) content of the erythrocyte membrane in this experiment. The results showed that AlCl 3 elevated the systolic and mean arterial blood pressure of rats, increased the osmotic fragility, decreased the percentage of membrane protein, inhibited the activities of Na + /K + -ATPase, Mg 2+ -ATPase, Ca 2+ -ATPase, CAT, SOD and GSH-pX, and increased the MDA content of erythrocyte membrane. These results indicate that AlCl 3 may induce hypertension by disturbing the function of erythrocyte membrane.
研究领域:
药理学
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毒理学
·
心血管研究
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Jian-Qing Liu,Ye-Bin Feng,Yin-Feng Xiao,Lu-Fang Chen,You-Xia Huang,Shui-Sen Lin,Yin Zhang,Qiu-Xia Xu,Shao-Bin He,Wei Chen
Glucose isomerase (GI), also known as D-xylose isomerase, plays a crucial role in converting glucose to fructose, widely applied in the food and agricultural industries. However, current GI activity detection methods suffer from limited sensitivity and operational complexity. Herein, we propose a novel detection strategy by coupling GI-catalyzed isomerization with a self-cascade copper oxide (CuO) nanozyme system. This cascade leverages the dual fructose oxidase-like and peroxidase-like activities of CuO nanozymes to achieve efficient signal amplification. The method offers a broad linear range (0.3–9.375 U/mL) and a low detection limit (0.11 U/mL), outperforming existing detection techniques. Spiking recovery experiments were performed on the glucose isomerase extract derived from Escherichia coli cultures, the culture medium, and commercial glucose isomerase preparations, yielding recoveries ranging from 86.22 % to 107.34 %. Given its high sensitivity, simplicity, and practicality, this method shows great promise for screening GI-producing bacterial strains and for the detection of GI activity in various applications.
研究领域:
纳米生物技术
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酶工程
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生物传感器
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食品生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Shuairu Zhu,Chao He,Hongwei Xu,Mei Yang,Wanding Cui,Xiaohua Yuan,Binwu Ying
Nanozymes, owing to their multi-enzymatic mimicking activities, have been widely applied in antibacterial fields. However, the effects of metal-doping elements on the structure-activity relationship of most layered double hydroxide (LDH) remain insufficiently understood. Here, we synthesized a series of Co-Fe-Cu ternary LDH nanosheets with varying Cu doping ratios, referred to as CoFeCu-LDH. These CoFeCu-LDH nanosheets exhibit four distinct enzymatic activities, including POD, oxidase (OXD), glutathione peroxidase (GPx), and glutathione oxidase (GSHOx). Notably, Cu doping produces a pronounced synergistic effect, significantly enhancing the enzymatic activities of CoFe-LDH. Specifically, CoFeCu-35 % demonstrates strong POD-like and GPx-like activities, while its OXD-like and GSHOx-like activities are comparatively weaker. Density functional theory calculations indicate that within the CoFeCu-35 % crystal structure, the energy barrier for reactions occurring at the copper site for POD and GPx is significantly lower than that at the cobalt or iron sites. Plate coating experiments further validate that CoFeCu-35 %, in conjunction with a small amount of H 2 O 2 , exhibits a stronger antibacterial effect than CoFeCu-35 % alone. The copper-doped samples effectively induce the production of reactive oxygen species more efficiently than pure CoFe-LDH, leading to enhanced antibacterial activity. Metabolic analyses provide additional insights into these underlying mechanisms. This study not only offers systematic insights into the structural doping design of multi-enzymatically active LDH materials but also presents promising strategies for the development of novel LDH-based antimicrobial agents.
研究领域:
纳米技术
·
材料科学
·
生物无机化学
·
抗菌剂
·
酶模拟
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产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2018
·
IF 3.849
Kexin Hou,Xuebing Yang,Meili Bao,Fengli Chen,Hao Tian,Lei Yang
An efficient approach to isolate fruit oil from Idesia polycarpa using homogenate-circulating ultrasound-assisted aqueous enzymatic extraction (H-CUAEE) was developed. An enzyme cocktail comprising cellulase, hemicellulase, and pectinase proved to be the most effective in extracting oils. Nine potential variables that possibly affected the extraction yields – the liquid-solid ratio, incubation temperature, incubation time, amount of enzyme, homogenate time, ultrasound irradiation power, mark-space ratio of ultrasound irradiation, rate of agitation, and pH – were investigated using a Plackett–Burman design in order to screen out the significant variables. The liquid-solid ratio, incubation temperature, and incubation time were significant on a statistical basis and these three parameters were studied through a Box–Behnken design for the prediction of the best experimental conditions that maximize extraction yield of the fruit oil. A maximum fruit oil recovery of 79.36% with an error of 0.17% was achieved under the optimal extracting conditions: liquid-solid ratio of 7.13, the incubation time of 2.94 h, and the incubation temperature of 48.74 °C. The H-CUAEE process has expansive prospects for the preparation of oils from oil plants.
研究领域:
植物科学
·
食品科学
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生物技术
·
化学工程
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）