NMR IN BIOMEDICINE
·
2025
·Vol. 38
·期 8
·e70093
·
IF 2.7
Zhongyichen Huang,Qiting Wu,Jing Zhang,Hao Tang,Gang Wu,Yao Zhang,Yi Wang,Dong Liang,Xiaoming Li,Yin Wu
Diabetes mellitus (DM) causes skeletal muscle damage. Energy metabolism alterations in diabetic muscles remain to be elucidated. Chemical exchange saturation transfer (CEST) MRI is increasingly adopted in imaging muscle energy metabolism. This study aimed to investigate energy metabolism alterations in diabetic muscles using CEST MRI. Six adult New Zealand rabbits with alloxan-induced DM were scanned on a 5 T MR imager before and 2, 4, 6, and 8 weeks after the DM model induction, with six normal rabbits as controls. T 2 -weighted imaging, B 1 field mapping, and CEST imaging under four B 1 amplitudes of 0.75, 1.0, 1.25, and 1.5 μT were performed, respectively. Blood glucose level was measured before each MRI experiment. Inverse magnetization transfer ratio analysis was adopted to quantify CEST effects of two energy metabolites of creatine (Cr) and phosphocreatine (PCr), from which their ratio was calculated. B 1 inhomogeneity was corrected using a smoothing spline interpolation algorithm. pH-sensitive CrCEST ratiometric was measured by dividing CrCEST under B 1 of 1.25 μT with that under 0.75 μT. Paired student's t -test and one-way ANOVA were conducted with p < 0.05 considered statistically significant. Blood glucose level measurements confirmed the successfully induced DM model. The controls showed no significant changes in Cr- and PCr-weighted CEST signals throughout the study. In contrast, a significant increase of Cr-weighted CEST signals started in 2 weeks ( p = 0.049) and became more pronounced in Week 6 ( p = 0.045) and Week 8 ( p = 0.002) after the DM model induction, whereas PCr-weighted CEST signals remained constant among multiple time points, leading to significantly reduced PCr/Cr in Week 6 ( p = 0.047) and Week 8 ( p = 0.011). Meanwhile, CrCEST ratio did not show significant alterations ( p > 0.05). The significantly increased Cr-weighted CEST and reduced PCr/Cr with time implied disrupted energy metabolism in diabetic muscles, providing supplementary information for better understanding of DM-related muscle impairments from the metabolic level.
研究领域:
生物医学工程
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内分泌学
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放射医学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2024
·
IF 5.8
Huiling Zhong,Zhen Zhang,Mohong Wang,Yifei Fang,Ke Liu,Junqiang Yin,Jun Wu,Jianhang Du
Acute severe trauma is often associated with rapid blood loss and a high risk of infection. Based on these concerns, this study successfully constructed a multifunctional dual-layer bioactive sponge PCCT with rapid hemostatic and infection-preventing ability. Its external surface is an electrospun poly(lactic acid) (PLA) nanofiber thin film layer, which ensures its high air permeability and effectively protects against external bacterial invasion. In vitro results showed that the film is effectively resistant to invasion by typical Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. The inner sponge layer was formed by chlorogenic acid (CGA) grafted with chitosan (CS) and loaded with tranexamic acid (TA). The abundant cationic groups on the sponge interacted with negatively charged erythrocytes and achieved rapid hemostasis at the wound site under the action of TA. In addition, the high porosity and bioactivity of the CS-CGA sponge scaffold endowed the hydrogel with good water absorption, antibacterial properties and anti-inflammatory activity, which effectively accelerated the healing of acute infected wounds in rats and demonstrated favorable biosafety.
研究领域:
生物材料学
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创伤修复
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药剂学
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DOI
PubMed
高影响因子
CHEMICAL GEOLOGY
·
2020
·
IF 3.363
Weiyan Que,Bihe Wang,Feili Li,Xiujuan Chen,Hui Jin,Zanfang Jin
The accumulation of heavy metals by freshwater algae becomes more complex in the presence of dissolved organic matter but it is unclear how and to what extent. In this study, we comparatively assessed the effects of malic acid and citric acid on the bioaccumulation of Pb by Chlorella pyrenoidosa . A 6 h exposure experiment showed that adding organic acid (OA) to an algae–Pb binary system prolonged the adsorption equilibrium time and that the extended time was related to the concentration and number of carboxyl groups of the OA. A pseudo-second-order model fit the kinetic data well in the presence of OAs. The normality of carboxyl groups was negatively correlated with the bioaccumulation rate, k 2 , but positively correlated with the maximum bioaccumulation capacity, q max , of Pb following a linear equation, Y = 121.3x + 52.2, R 2 = 0.9877. Neither the Freundlich model nor the Langmuir model could fit the isotherm data in the presence of OA, suggesting that a new mechanism might exist. The quantitative relationship between bioaccumulated Pb and carboxyl group of OA indicated a stoichiometric relationship, which seemed to support the theory of a ternary complex of Pb, OA, and algal surface. Our results have added clarity to the current understanding of the accumulation of heavy metals by algae in freshwater ecosystems .
研究领域:
环境科学
·
水生生物学
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DOI
高影响因子
POULTRY SCIENCE
·
2020
·
IF 2.659
Mingfa Sun,Hongchao Jiao,Xiaojuan Wang,Victoria A. Uyanga,Jingpeng Zhao,Hai Lin
Crystalline amino acids ( AAs ) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl ( Ly s) and DL-methionine ( DL-Met ) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets ( 80CLM , 60CLM ), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group ( P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group ( P < 0.05), but increased villi height and B 0 AT mRNA expression level ( P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group ( P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens.
研究领域:
动物营养学
·
家禽科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Bo Wang,Shuai Zheng,Yan Huang,Ying Wang,Zhenyu Zhu,Ran Ma,Yu Zhao,Xijie Yin,Jing Su,Juan Xiong,Benli Zhang,Youping Zhou
The 18O/16O (and 15N/14N) ratio of natural nitrate (NO3–) and nitrite (NO2–) can be used to extract valuable information about their source and fate as environmental contaminants, their metabolism as macronutrients in plants and animals, and their behavior in the N biogeochemical cycle. We developed an accurate, precise, sensitive (minimum sample size: 0.2 μg NO3–-equivalent), and reliable (minimal oxygen exchange, loss, or gain) method to selectively isolate and purify nitrate and nitrite from natural water, soil, air, and plant materials by strong anion exchange (SAX) for low- to normal-salinity samples or strong cation exchange (SCX) for high-salinity samples, followed by quantitative conversion to their respective benzyl esters, which can be separated and individually analyzed for δ18O (and potentially δ15N) by gas chromatography (GC)/pyrolysis/GC/isotope-ratio mass spectrometry (IRMS). The method compares favorably with the currently popular bacterial denitrification and chemical reduction methods, in terms of sensitivity and reliability, and has the potential to simultaneously measure δ15N and δ18O of nitrate and nitrite from natural samples of various origins.
研究领域:
环境科学
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生物地球化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Lili Guo,Baozhu Yang,Datong Wu,Yongxin Tao,Yong Kong
Molecular self-assemblies offer a promising strategy for the synthesis of advanced materials for the construction of novel chiral sensing systems, and latest innovations on such self-assemblies are focused on simple building blocks in biology such as nucleic acids, lipids, and peptides. Herein, the self-assemblies of diphenylalanine (FF) and oxalic acid (OA) were prepared as the chiral sensing device for the recognition of tryptophan (Trp) isomers. Interestingly, the self-assemblies composed of OA and FF with different charging states (neutral, positively charged, and negatively charged) exhibited quite different morphologies, resulting in significantly different chiral recognition ability toward the Trp isomers. Also, in this work, the temperature sensitivity and chiral selectivity of the proposed FF-OA self-assemblies were also studied. From a practical point of view, the FF-OA self-assemblies were finally applied for the determination of precise levels of d-Trp in the racemic mixture of Trp isomers.
研究领域:
生物化学
·
材料科学
·
分析化学
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DOI
PubMed
高影响因子
Sustainable Energy & Fuels
·
2018
·Vol. 2
·期 7
·1530-1541
·
IF 0
Xincheng Wang,Yongji Song,Chongpin Huang,Bin Wang
Lactic acid (LA) is a versatile platform chemical for the production of biodegradable polymers as well as starting materials for the pharmaceutical industry. In this study, crystalline niobium phosphates directed by various surfactants were prepared by a facile sol–gel method and studied as heterogeneous Lewis acid catalysts for the direct conversion of triose sugars to LA under aqueous conditions. Metal oxides were used to alter the surface acidity, and pyridine FTIR analyses demonstrated the presence of both Lewis and Brønsted acid sites, which played essential roles in the conversion of biomass sugars. The incorporation of SnO2 and the use of surfactants significantly lowered the Brønsted-to-Lewis site ratio. P–OH groups were determined to be the origin of the Brønsted acid sites, while the partially hydrolyzed framework of tin and niobium species led to the formation of Lewis acid sites. The cationic surfactant-modified samples with highly crystalline structures outperformed the amphiphilic surfactant-modified samples. The optimum cetyltrimethylammonium bromide-modified catalyst afforded an LA yield of ca. 92% with the complete dihydroxyacetone (DHA) conversion at 160 °C. Furthermore, the isomerization of pyruvaldehyde (PA) was found to be the rate-determining step, while the rehydration of PA to DHA could occur in the current reaction system. A possible reaction mechanism involving the evolution of two key catalytic intermediates was proposed. In addition, crystalline niobium phosphates were also effective in the aqueous dehydration of sugars to furan derivatives with a 44% 5-hydroxymethylfurfural yield from glucose after 1 h. The current study can hopefully serve as a model for the development of novel solid acid catalysts for the conversion of sugars to platform chemicals.
研究领域:
化学工程
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催化科学
·
生物质转化
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DOI
JOURNAL OF MOLECULAR LIQUIDS
·
2019
·
IF 4.561
Sile Shi,Mengqi Yan,Bao Tao,Weiping Luo
In this study, the solid-liquid equilibrium (SLE) data of succinic acid (SA) and glutaric acid (GA) and adipic acid (AA) in N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMAC), dimethyl sulfoxide (DMSO), 1-Methyl-2-pyrrolidinone (NMP) and sulfolane were determined by using the method of dynamic laser at atmospheric pressure. The experimental SLE data indicates that within the temperature range studied (286.25–358.75 K), the solubilities of SA, GA and AA in above five solvents is proportional to the temperature. Meanwhile, three thermodynamic models (Apelblat model, λh model, the modified nonrandom two liquid (NRTL) activity coefficient model) were used to correlate the experimental data, the results show that the correlated data is generally consistent with the experimental data. The result of Akaike Information Criterion (AIC) analysis filter out the best model for every system. In addition, the thermodynamic functions (dissolution enthalpy, entropy, Gibbs energy) were obtained from the SLE data by calculating the van't Hoff equation.
研究领域:
化学工程
·
物理化学
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DOI
高影响因子
CHEMOSPHERE
·
2018
·
IF 4.427
Jing Xu,Heng Zhang,Tao Luo,Zizheng Liu,Jun Xia,Xiang Zhang
The effects of co-existing nitrogen species in surface water on the phototransformation of organoarsenical p -arsanilic acid ( p -ASA) have been investigated using a xenon lamp as a simulated solar light source. Significant enhancements of p -ASA phototransformation efficiency were observed in the presence of nitrate and nitrite, increasing with the concentration of these species and pH, whereas ammonia showed no obvious effect. The products, including inorganic arsenic species and organic derivatives, have been analyzed in order to reveal the phototransformation pathways. In the nitrate and nitrite systems, only small proportions of inorganic arsenic species were generated, with the majority of p -ASA being converted into other organoarsenical derivatives through hydroxylation, nitration, and nitrosation. Phototransformation of p -ASA in collected natural surface water was also observed. This work has implications for the phototransformation of p -ASA in nitrogen-contaminated surface water.
研究领域:
环境科学
·
水污染控制
·
光化学反应
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产品关联
DOI
PubMed
高影响因子
POLYMER COMPOSITES
·
2016
·
IF 2.004
Peng Wang,Pengzheng Liu,Fan Xu,Xiaoxia Fan,Huipin Yuan,Hong Li,Yonggang Yan
Ternary biocomposites of dicalcium phosphate (DCP), calcium sulfate (CS), and poly(amino acid) copolymer (PAA) could be used as a promising degradable biomimic bone substitute. Using an in vitro degradation model, we evaluated the influence of polymerization conditions, initial amino acids, and (DCP + CS)/PAA ratio on the degradation of the resulting materials. PAA degradation was affected by both the polymerization conditions and the initial amino acids, and the degradation of the DCP/CS/PAA composites was affected by the (DCP + CS)/PAA ratio. Using a certain initial amino acids (75% ε-aminohexanoic acid, 19% γ-aminobutanoic acid, 4% l -proline, and 2% l -lysine) and polymerization conditions (i.e., 210°C for 2.5 h) as the polymeric phase, (DCP + CS)/PAA biocomposites were prepared with 70%, 60%, and 40% PAA. All of the biocomposites were degradable, which resulted in the release of ions/amino acids and a slight pH fluctuation of 6.76 to 7.20. In addition, these biocomposites were biocompatible based on the proliferation of L929 but varied with the degradation rates. These results suggest the potential to control the degradation rates of (DCP + CS)/PAA biocomposites for bone regeneration. POLYM. COMPOS., 39:E122–E131, 2018. © 2016 Society of Plastics Engineers
研究领域:
生物材料学
·
组织工程学
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产品关联
DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 1
·e70015
·
IF 3.4
Jingxuan Yang,Yi Wu,Ziye Jiang,Lili Jiao,Yang Wang
This study aimed to investigate the potential hypoglycemic mechanism of red ginseng acidic polysaccharides (RGAP) from the perspective of fatty acid (FA) regulation. A high-glucose/high-fat diet in conjunction with streptozotocin administration was employed to establish type 2 diabetes mellitus (T2DM) rat models, and their fecal FAs were detected using the liquid chromatography-mass spectrometry (LC-MS) method. RGAP treatment alleviated the polyphagia, polydipsia, weight loss, and hyperglycemia observed in T2DM rats. FA profile was disturbed by T2DM modeling, and 11 marker FAs were selected from statistical analysis, whose intensities were reversely changed by RGAP administration. Among these marker FAs, short-chain FAs were negatively correlated with the fasting blood glucose (FBG) level, while positive correlations were observed between long-chain FA and the FBG level. Combined with the metabolite-enzyme-gene network analysis, we inferred that the mechanistic mechanism RGAP on T2DM may be associated with the regulation of FA metabolism and inflammation-related signaling pathways. This study confirmed the regulatory effect of RGAP on fecal FA, which can provide a scientific basis and new ideas for developing red ginseng as a functional food for supplementary treatment of T2DM.
研究领域:
营养学
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代谢组学
·
药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2019
·
IF 2.29
Xiaojie Wang,Shanshan Zhu,Xiaopo Wang
The experimental measurements for liquid viscosities of four fatty acid methyl esters, including methyl hexanoate, methyl heptanoate, methyl caprylate, and methyl nonanoate, were carried out with a falling-cylinder viscometer. For the present measurement, the temperature ranges were from 292 K to 362 K and the pressure was reached to 30 MPa. The combined expanded relative uncertainty of the viscosity measurements was within 0.03 with 0.95 confidence level. The viscosity data for each studied esters were correlated by Andrade-Tait equation, and the average absolute relative deviation between the experimental data and calculated values is 0.31%, 0.44%, 0.38%, and 0.25% for methyl hexanoate, methyl heptanoate, methyl caprylate, and methyl nonanoate, respectively. The maximum absolute relative deviation is 1.09%, 1.28%, 1.28%, and 0.75%.
研究领域:
化学工程
·
物理化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）