JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
·
2023
·
IF 5.2
Pei Li,Zhishuang Yang,Ting Lei,Yujie Dai,Yang Zhou,Dekang Zhu,Hongyan Luo
Objectives: To elucidate the role of a novel carbapenem-hydrolysing class D β-lactamase (RAD-1) from Riemerella anatipestifer. Methods: We applied WGS and bioinformatic analysis to screen putative β-lactamase genes in R. anatipestifer SCVM0004. A putative class D β-lactamase gene was cloned into pET24a and transferred into Escherichia coli BL21 (DE3) for antibiotic susceptibility determination and protein purification. Meanwhile, the purified native protein was used to determine the enzymatic activities. Results: A class D β-lactamase, RAD-1, was identified in the genome of R. anatipestifer SCVM0004. It was distinct from all characterized class D β-lactamases (≤42% amino acid sequence identity). Searching in GenBank showed that blaRAD-1 was widely disseminated among R. anatipestifer. Genomic environment analysis indicated that the chromosomal structures of blaRAD-1-located regions were relatively conserved. Expression of RAD-1 in E. coli results in elevated MICs for various β-lactam antibiotics, including penicillins, extended-spectrum cephalosporins, a monobactam and carbapenems. Moreover, kinetic analysis of purified RAD-1 revealed: (i) high-level activity against penicillins; (ii) highest affinity for carbapenems; (iii) moderate hydrolysis of extended-spectrum cephalosporins and a monobactam; and (iv) no activity for oxacillin and cefoxitin. Conclusions: This study identified a novel chromosomally located class D carbapenemase RAD-1 (Bush-Jacoby functional group 2def) in R. anatipestifer SCVM0004. Moreover, bioinformatic analysis confirmed that the RAD-1 was widely prevalent and conserved in R. anatipestifer.
研究领域:
微生物学
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基因组学
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抗菌素耐药性
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生物信息学
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DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2025
·
IF 4.2
Di Sun,Wenze Cheng,Zheng Li,Hui Yao,Mafeng Liu,Mingshu Wang,Shun Chen,Yukun Zhu,Jingming Wang,Yuan Deng,Funeng Xu,Xumin Ou,Renyong Jia,Dekang Zhu,Xinxin Zhao,Qiao Yang,Ying Wu,Shaqiu Zhang,Juan Huang,Bin Tian,Yu He,Zhen Wu,Anchun Cheng
Duck hepatitis A virus type 3 (DHAV-3) has emerged as a dominant pathogen responsible for duck viral hepatitis, leading to significant economic losses in the duck industry. Given that current control strategies, primarily reliant on vaccination, often face limitations, this study investigates a host-directed therapeutic (HDT) strategy focused on targeting host factors essential for DHAV-3 replication. We identified the heat shock protein family A member 8 (HSPA8) as a key interactor of the viral structural protein VP0 through GST pull-down and mass spectrometry. Functional assays confirmed that HSPA8 significantly promotes DHAV-3 replication; its overexpression enhanced viral yield, while its inhibition suppressed it. Building on these findings, we explored oxymatrine (OMT), a natural compound derived from Sophora flavescens (Kushen), and found that it effectively downregulates HSPA8 expression, thereby inhibiting viral replication in vitro . In a duckling model, OMT treatment provided significant protection, dose-dependently improving survival rates, reducing viral loads, and alleviating DHAV-3-induced liver injury and apoptosis. Transcriptomic profiling revealed that OMT mitigates liver damage through dual mechanisms: by suppressing the overactivated RIG-I-like receptor signaling pathway and inhibiting the apoptosis pathway. Our work establishes HSPA8 as a promising pan-picornaviral target and identifies OMT as a novel and effective HDT candidate against DHAV-3 infection.
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PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Ligang Yu,Ying Pang,Guang Shen,Baoqing Bai,Yukun Yang,Maomao Zeng
This work aimed to investigate the effects of frozen storage on volatile compounds of white meats (chicken and duck) and red meats (pork, beef, and mutton). The samples were stored at −18 °C for 0, 2, 4, 10, 18 weeks, and volatile compounds were analyzed by headspace solid-phase microextraction coupled with gas chromatography–mass spectrometry. Results indicated that the total amounts of volatile compounds increased with frozen storage duration of meats. The correlations were observed between frozen storage duration and levels of 2-ethyl-1-hexanol, tetradecane, nonanal, decanal, octanal, tridecanal, benzaldehyde, pentadecane, propanoic acid,2-methyl-,3-hydroxy-2,2,4-trimethylpentyl ester, heptadecane, and hexanal ( r = 0.7456–0.9873). Levels of octanal and propanoic acid,2-methyl-,3-hydroxy-2,2,4-trimethylpentyl ester in white meat and benzaldehyde in red meat versus frozen storage duration fitted very well with zero-order reactions. Therefore, it was concluded that changes in volatile compounds derived from lipid oxidation may be used as indicators of quality deterioration during frozen storage of meat.
研究领域:
食品科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Caiying Zhang,Xueru Wang,Shaoxing Pi,Zejing Wei,Chang Wang,Fan Yang,Guyue Li,Gaohui Nie,Guoliang Hu
Cadmium (Cd) and excessive molybdenum (Mo) are detrimental to animals, but the combined nephrotoxic impacts of Cd and Mo on duck are still unclear. To evaluate the combined impacts of Cd and Mo on autophagy via Cytochrome P450s (CYP450s)/reactive oxygen species (ROS) pathway, duck renal tubular epithelial cells were treated with 3CdSO 4 ·8H 2 O (4.0 μM Cd), (NH 4 ) 6 Mo 7 O 24 ·4H 2 O (500.0 μM Mo), butylated hydroxy anisole (BHA) (100.0 μM) and combination of Cd and Mo or Cd, Mo and BHA for 12 h, and combined cytotoxicity was investigated. The results indicated that Mo or/and Cd induced CYP1A1, CYP1B1, CYP2C9, CYP3A8 and CYP4B1 mRNA levels, decreased superoxide dismutase (SOD), catalase (CAT) activities and glutathione peroxidase (GSH-Px) content, and increased malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) contents. Besides, Mo or/and Cd elevated the number of autophagosome and microtubule-associated protein light chain 3 (LC3) puncta, upregulated mRNA levels of Beclin-1, LC3A, LC3B, Atg5 and adenosine 5′-monophosphate (AMP)-activated protein kinase α1 (AMPKα-1), inhibited Dynein, p62 and mammalian target of rapamycin (mTOR) mRNA levels, increased Beclin-1 and LC3II/LC3I protein levels. Moreover, the changes of these factors in Mo and Cd co-treated groups were more apparent. Additionally, BHA could efficiently alleviate the changes of above these indicators co-induced by Mo and Cd. Overall, these results manifest Cd and Mo co-exposure may synergistically trigger autophagy via CYP450s/ROS pathway in duck renal tubular epithelial cells.
研究领域:
毒理学
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细胞生物学
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环境科学
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DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2021
·
IF 3.352
Jian Chen,Muhua Liu,Haichao Yuan,Xiongfei Chen,Jinhui Zhao
ABSTRACT Rapid detection of antibiotic residues in duck meat is of great significance for strengthening food safety and quality supervision of duck meat and fighting against inferior products in the duck meat market. The objective of the current paper was to evaluate the potential of synchronous fluorescence spectroscopy ( SFS ) coupled with chemometric methods for the rapid detection of sulfamethazine ( SM2 ) and ofloxacin ( OFL ) residues in duck meat.The SFS spectral data from duck meat containing different concentrations of SM2 and OFL were preprocessed by baseline offset. The detection conditions, including the adding amounts of β-mercaptoethanol solution and o-phthalaldehyde solution, as well as the reaction time, were optimized by a single factor experiment for obtaining a better detection effect, and their optimal values were 400 μL , 25 μL , and 40 min, respectively. By comparing 2 chemometric models based on peak-height algorithm and peak-area algorithm, the prediction model based on peak-height algorithm was a better quantitative model with correlation coefficient for the prediction set ( Rp ) of 0.9031 and 0.9981, the root mean error for the prediction set ( RMSEP ) of 7.9509 and 0.5267 mg/kg, recovery of 81.7 to 155.1% and 96.4 to 111.2%, and relative standard deviation ( RSD ) of 4.1 to 6.7% and 2.9 to 6.8% to predict SM2 and OFL residues in duck meat, respectively. Overall, the results of this investigation showed that SFS technique was an effective and rapid tool for the detection of SM2 and OFL residues in duck meat.
研究领域:
食品科学
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分析化学
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光谱学
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DOI
PubMed
高影响因子
Toxins
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2022
·Vol. 14
·期 12
·876
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IF 5.075
Yanan Wang,Jiayu Wu,Lingfeng Wang,Ping Yang,Zuhong Liu,Shahid Ali Rajput,Mubashar Hassan,Desheng Qi
Aflatoxin B1(AFB1) exists widely in feed and food with severe hazards, posing a serious threat to human and animal health. Epigallocatechin gallate (EGCG) and glutathione (GSH) have been reported as having anti-oxidative and other functions. The present study aimed to investigate the detoxification effect of EGCG and GSH alone or in combination on AFB1exposure in ducklings. Fifty one-day-old male ducklings were randomly assigned into five experimental groups (n= 10): 1. Control (CTR); 2. 0.3 mg/kg BW AFB1(AFB1); 3. 0.3 mg/kg BW AFB1+ 100 mg/kg BW EGCG (AFB1+ EGCG); 4. 0.3 mg/kg BW AFB1+ 30 mg/kg BW GSH (AFB1+ GSH); 5. 0.3 mg/kg BW AFB1+ 100 mg/kg BW EGCG + 30 mg/kg BW GSH (AFB1+ EGCG + GSH). The experiment lasted for seven days. Compared with the CTR group, AFB1reduced growth performance, total serum protein and albumin content, increased serum enzyme activity (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ-glutamyl transpeptidase), and caused pathological damage to the ducklings’ livers. AFB1exposure increased malondialdehyde content and decreased superoxide dismutase, total antioxidant capacity, catalase, glutathione peroxidase activities, and glutathione content in the liver. EGCG and GSH alone or in combination mitigated these adverse effects. Meanwhile, EGCG and GSH attenuate apoptosis of hepatocytes, and regulated AFB1-induced changes in the abundance of genes contained in the Keap1/Nrf2 signalling and apoptotic pathways. Collectively, these results suggest that EGCG and GSH alleviate the hepatocyte injury induced by AFB1by inhibiting oxidative stress and attenuating excessive mitochondria-mediated apoptosis.Keywords:aflatoxin B1;duckling;epigallocatechin gallate;glutathione;oxidative stress;Keap1/Nrf2 signalling;apoptosisKey Contribution:EGCG and GSH alleviate AFB1-induced liver injury in ducklings by increasing antioxidant capacity and antagonising apoptosis.
研究领域:
兽医学
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毒理学
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药理学
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Hongyan Luo,Zhishuang Yang,Ting Lei,Caixia Li,Zuoyong Zhou,Mingshu Wang,Dekang Zhu,Pei Li,Anchun Cheng
Carbapenem resistance's global proliferation poses a significant public health challenge. The primary resistance mechanism is carbapenemase production. In this study, we discovered a novel carbapenemase, RATA, located on the chromosome of Riemerella anatipestifer isolates. This enzyme shares ≤52 % amino acid sequence identity with other known β-lactamases. Antimicrobial susceptibility tests and kinetic assays demonstrated that RATA could hydrolyze not only penicillins and extended-spectrum cephalosporins but also monobactams, cephamycins, and carbapenems. Furthermore, its activity was readily inhibited by β-lactamase inhibitors. Bioinformatics analysis revealed 46 bla RATA -like genes encoding 27 variants in the NCBI database, involving 21 different species, including pathogens, host-associated bacteria, and environmental isolates. Notably, bla RATA -positive strains were globally distributed and primarily collected from marine environments. Concurrently, taxonomic analysis and GC content analysis indicated that bla RATA orthologue genes were predominantly located on the chromosomes of Flavobacteriaceae and shared a similar GC content as Flavobacteriaceae . Although no explicit mobile genetic elements were identified by genetic environment analysis, bla RATA-2 possessed the ability of horizontal transfer in R. anatipestifer via natural transformation. This work's data suggest that RATA is a new chromosome-encoded class A carbapenemase, and Flavobacteriaceae from marine environments could be the primary reservoir of the bla RATA gene.
研究领域:
微生物学
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分子生物学
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传染病学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2021
·
IF 4.952
Lin Yu,Hui Xue,Chunhong Xiong,Xiaojuan Xin,Ping Wang,Feng Feng,Danhui Cao,Yonggang Tu,Yan Zhao
In this paper, the changes of physico-chemical characteristics, microstructures and secondary structures of duck egg white (EW) gels under the action of baijiu (Chinese liquor) were studied via the texture analyzer, environmental scanning electron microscope (ESEM) and Fourier transform infrared spectroscopy (FT-IR). Results showed that the hardness, centrifugal water holding capacity (WHC) and surface hydrophobicity of the EW gels increased gradually with the pickling time. ESEM indicated that the EW gels exhibited an increasingly compact network structure. The ionic bond and disulfide bond took the leading role in maintaining the gel network. SDS-PAGE showed that lysozyme disappeared first, and other proteins except ovomucoid disappeared gradually. The absolute value of zeta potential reached the maximum on the 8th day and then decreased slightly. The overall trend of α- helix, β- turn, β- sheet and the random coil in the secondary structure of the gel increased.
研究领域:
食品科学
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化学
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生物物理学
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DOI
高影响因子
BMC Veterinary Research
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2019
·Vol. 15
·期 1
·1-12
·
IF 1.792
Du Hongxu,Bai Jingying,Wang Jinli,He Miao,Xiong Wen,Yuan Wenjuan,Qiao Mingyu,Ming Ke,Wu Yi,Wang Deyun,Hu Yuanliang,Liu Jiaguo
Background Duck viral hepatitis (DVH) is an acute disease of young ducklings with no effective veterinary drugs for treatment. Gynostemma pentaphyllum is a well-known traditional Chinese medicine that plays an important role in the treatment of various diseases. Gypenoside (GP), one of the main ingredients of Gynostemma pentaphyllum, was reported with good hepatoprotective effects. However, its low solubility limits its application in the clinics. To improve its solubility and bioactivity, a phosphorylated derivative of gypenoside (pGP) was prepared by the sodium trimetaphosphate-sodium tripolyphosphate (STMP-STPP) method. An infrared spectroscopy method was applied to analyse the structures of GP and pGP. Then, a methyl thiazolyl tetrazolium (MTT) colorimetric assay was applied to study the hepatocyte protective efficacy of these two drugs against duck hepatitis A virus type 1 (DHAV-1) infection, and qPCR, TUNEL labelling and flow cytometry methods were used to study the relevant hepatocyte protective in vitro.Results The infrared spectroscopy detection results showed that the phosphorylation modification of GP was successful. The MTT colorimetric assay results showed that both GP and pGP possessed good hepatocyte protective efficacy in vitro, and pGP performed better than GP when the drug was added before or after virus inoculation. Furthermore, the qPCR results revealed that both drugs could effectively inhibit the adsorption (when adding GP and pGP pre-virus inoculation), replication and release of DHAV-1, and the viral inhibition rate of pGP was greater than that of GP. The subsequent TUNEL labelling and flow cytometry assays showed that both GP and pGP could significantly inhibit duck embryo hepatocyte apoptosis induced by DHAV-1, and the inhibition effect of pGP was much stronger than that of GP.Conclusion sGP exerts good hepatocyte protective efficacy not only by inhibiting the proliferation of DHAV-1 but also by inhibiting duck embryonic hepatocyte apoptosis induced by DHAV-1, and phosphorylation modification significantly improves the antiviral and the anti-apoptotic effects of GP. Therefore, pGP has the potential to be developed into a novel drug against DHAV-1 infection.
研究领域:
兽医学
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药理学
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病毒学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Di Shao,Shuyun Bi,Rui Zhao,Xiaoyue Sun,Xu Li,Jingfu Yu
Two sensitive surface-enhanced Raman spectroscopy (SERS) analytical methods for dinitolmide and toltrazuril were developed by silver nanoparticles (AgNPs). The dinitolmide and toltrazuril were determined in 0.0565 mol L −1 and 0.0847 mol L −1 NaOH solutions respectively. The enhancement factors (EF) were 1.76 × 10 5 for dinitolmide and 1.27 × 10 5 for toltrazuril. Under optimum conditions, the methods had good linear relationship in the concentration ranges of 0.30–33.30 nmol L −1 for dinitolmide ( I SERS = 1522.52 c + 27,890.46, c :nmol L −1 , R 2 = 0.998) and 0.30–66.70 nmol L −1 for toltrazuril ( I SERS = 1334.29 c + 46,347.83, c :nmol L −1 , R 2 = 0.999). The limits of detection (LOD) (S/N = 3) were 0.915 nmol L −1 for dinitolmide and 1.03 nmol L −1 for toltrazuril. The effects of interferences on the determinations of dinitolmide and toltrazuril were studied. These SERS methods were successfully used to measure the content of dinitolmide and toltrazuril in the duck and chicken samples. The relative standard deviation (RSD) for the detection of dinitolmide and toltrazuril samples could reach 1.45 % ( n = 5). The recoveries were 95.67%–105.39% ( n = 5) and 94.79%–99.44% ( n = 5) for dinitolmide and toltrazuril system respectively.
研究领域:
分析化学
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兽医学
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纳米技术
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Tianfeng Han,Hui Xue,Xiaobo Hu,Ruiling Li,Huilan Liu,Yonggang Tu,Yan Zhao
In this paper, the effects of NaOH, NaCl, and heat on the appearance, physical characteristics, and gel structure of duck egg white (EW) jointly induced by NaOH, NaCl, and heat were investigated. Results showed that the absolute value of zeta potential (TAV-ZP) of gel samples increased, hunter whiteness decreased, gel opacity decreased, and the microstructure of gel appeared as a loose network structure with increased NaOH addition. With the increase of NaCl addition, TAV-ZP of gel samples decreased, hunter whiteness increased, gel opacity increased, the microstructure of gel became dense. With the increase of heating temperature, TAV-ZP of gel samples decreased, hunter whiteness decreased, gel opacity increased, the microstructure of gel became dense first and then loosened. The results of SDS-PAGE, surface hydrophobicity, T 2 , hardness, cohesiveness indicated that protein molecules underwent cross-linking, aggregation, and unfolding during duck EW gelation jointly induced by NaOH, NaCl, and heat. The results of strain-stress curve showed that all gel samples had a good anti-compressed ability. The results of central composite design showed that the best treatment of gel was heating temperature 91 °C, NaOH 0.48%, and NaCl 0.3%.
研究领域:
食品科学
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化学
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生物物理学
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DOI
高影响因子
POULTRY SCIENCE
·
2021
·
IF 3.352
Chen Yang,Pei Luo,Shi-jian Chen,Zhi-chao Deng,Xin-liang Fu,Dan-ning Xu,Yun-bo Tian,Yun-mao Huang,Wen-jun Liu
ABSTRACT Resveratrol, a natural antioxidant, anti-inflammatory plant extract, was found to have a protective effect in poultry subjected to heat stress. In this study, we strove to characterize resveratrol on intestinal of duck exposed to acute heat stress and investigate the underlying mechanism. A total of 120 Shan-ma ducks (60 days old) were randomly divided into 2 groups. The control group was fed a basal diet, and the resveratrol group was fed a basal diet supplemented with 400 mg/kg resveratrol. Animals in 2 groups were kept at a temperature of 24°C ± 2°C for 15 d. Then, animals of both groups were placed in an artificial climate room at 39°C. Twelve ducks of each group were sacrificed for sampling at 0, 30, and 60 min, respectively. Results indicated that resveratrol increased the ratio of villus height to crypt depth, increased the number of goblet cells, and reduced the histopathological damage of jejunum caused by acute heat stress. Furthermore, the gene expression of heat shock proteins ( HSP60, HSP70 , and HSP90 ) and tight junction proteins ( CLDN1 and OCLN ) was significantly increased in the resveratrol group compared to that in the control groups. Simultaneously, resveratrol significantly activated the SIRT1-NRF1/NRF2 signaling pathways, improved ATP level of jejunum, and increased SOD and CAT antioxidant enzymes activities. In addition, we found that the NF-κB / NLRP3 inflammasome signaling pathways were repressed under acute heat stress. Meanwhile, supplement resveratrol further inhibited the NLRP3 inflammasome pathway, decreased protein level of NLRP3 and caspase1 p20, reduced the secretion of IL-1β. Taken together, our results indicate that resveratrol against the oxidative damage and inflammation injury in duck jejunum induced by heat stress via active SIRT1 signaling pathways.
研究领域:
动物科学
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营养学
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生理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）