PeerJ
·
2017
·
IF 2.177
Jihang Dai,Yu Sun,Lianqi Yan,Jingcheng Wang,Xiaolei Li,Jun He
The fibrosis that develops following laminectomy or discectomy often causes serious complications, and the proliferation of fibroblasts is thought to be the major cause of epidural fibrosis. 10-Hydroxycamptothecin (HCPT) has been proven to be efficient in preventing epidural fibrosis, but the exact mechanism is still unclear. NOXA is a significant regulator of cell apoptosis, which has been reported to be beneficial in the treatment of fibrosis. We performed a series of experiments, both in vitro and in vivo , to explore the intrinsic mechanism of HCPT that underlies the induction of apoptosis in fibroblasts, and also to investigate whether HCPT has positive effects on epidural fibrosis following laminectomy in rats. Fibroblasts were cultured in vitro and stimulated by varying concentrations of HCPT (0, 1, 2, 4 µg/ml) for various durations (0, 24, 48, 72 h); the effect of HCPT in inducing the apoptosis of fibroblasts was investigated via Western blots and TUNEL assay. Our results showed that HCPT could induce apoptosis in fibroblasts and up-regulate the expression of NOXA. Following the knockdown of NOXA in fibroblasts, the results of Western blot analysis showed that the level of apoptotic markers, such as cleaved-PARP and Bax, was decreased. The results from the TUNEL assay also showed a decreased rate of apoptosis in NOXA-knocked down fibroblasts. For the in vivo studies, we performed a laminectomy at the L1-L2 levels in rats and applied HCPT of different concentrations (0.2, 0.1, 0.05 mg/ml and saline) locally; the macroscopic histological assessment, hydroxyproline content analysis and histological staining were performed to evaluate the effect of HCPT on reducing epidural fibrosis. The TUNEL assay in epidural tissues showed that HCPT could obviously induce apoptosis in fibroblasts in a dose-dependent manner. Also, immunohistochemical staining showed that the expression of NOXA increased as the concentrations of HCPT increased. Our findings are the first to demonstrate that upregulation of NOXA by HCPT plays a key role in inducing fibroblast apoptosis and in reducing epidural fibrosis. These findings might provide a potential therapeutic target for preventing epidural fibrosis following laminectomy.
研究领域:
药理学
·
神经外科
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
POLYMER
·
2022
·
IF 4.432
Hanwen Lai,Xiaohui Zhang,Guangcheng Huang,Yadong Liu,Weihua Li,Shengxiang Ji
Directed self-assembly (DSA) of block copolymers (BCPs) is an effective approach for the fabrication of sub-10 nm features for semiconductor devices. Although many high-χ BCPs have been developed for DSA, the majority of them has a significant difference in surface energies (γs) between two blocks. Here we report the synthesis and self-assembly of poly (styrene- b -vinyl acetate) (PS- b -PVAc). PS- b -PVAc has a higher Flory-Huggins interaction parameter (χ) than poly (styrene- b -methyl methacrylate) (PS- b -PMMA) and the smallest achievable period ( L O ) is 18.2 nm. PS and PVAc have nearly equal γs and a perpendicular orientation of domains is obtained on random copolymer brushes under thermal annealing. DSA of PS- b -PVAc (SV-1 and SV-2) is successfully demonstrated in the thin films on chemical patterns, and the feature size down to 9.5 nm is obtained in DSA of SV-4 with 2 × density multiplication. These results demonstrate that PS- b -PVAc could be a candidate BCP for next-generation DSA lithography.
研究领域:
材料科学
·
纳米技术
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产品关联
DOI
高影响因子
AAPS PHARMSCITECH
·
2020
·Vol. 21
·期 8
·1-10
·
IF 2.401
Li Ruiming,Wang Yong,Yang Qiang,Lai Baolong,Zhou Xie,Feng Min
10-Hydroxycamptothecin (HCPT) is a DNA inhibitor of topoisomerase I and exerts antitumor activities against various types of cancer. However, reversible conversion from a pharmacologically active lactone form to an inactive carboxylate form of HCPT and poor water solubility hamper its clinical applications. To overcome these shortcomings, we designed a fine self-microemulsifying drug delivery system (SMEDDS) for HCPT to effectively protect HCPT in its active lactone form as well as improving dissolution rates. A formulation of HCPT-SMEDDS that contained ethyl oleate, D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), and polyethylene glycol 400 (PEG400) was optimized by using the central composite design and response surface methodology. Following 1:100 aqueous dilution of the optimized HCPT-SMEDDS, the droplet size of resulting microemulsions was 25.6 ± 0.7 nm, and the zeta potential was − 15.2 ± 0.4 mV. The optimized HCPT-SMEDDS appeared to stabilize the lactone moiety of HCPT with 73.6% being present in the pharmacologically active lactone forms in simulated intestinal fluid, but only 45.7% for free HCPT. Furthermore, the physically stable formulation showed the active lactone form predominated in HCPT-SMEDDS (> 95%) for 6 months under the accelerated storage condition. Meanwhile, the optimized SMEDDS formulation also significantly improved dissolution rates and membrane permeability of the lactone form of HCPT. Therefore, HCPT-SMEDDS involved designing for the ease of manufacture, and provided a potent oral dosage form for preserving its active lactone form as well as enhancing the dissolution rate.
研究领域:
药剂学
·
肿瘤学
·
生物制药
✓ 已验证
DOI
PubMed
高影响因子
FUEL
·
2023
·
IF 7.4
Junjian Xie,Jun Zhang,Xiaoyu Wang,Jiawei Xie,Bo Yang,Yuxuan Liang,Ji-Jun Zou,Qiuyu Zhang
JP-10 is the most widely used petroleum based fuel in military aircraft due to its high energy density and excellent cryogenic properties. However, the high price and low availability limit its application in civil aviation. Herein, JP-10 analogues fuel was first synthesized via one-pot Diels-Alder/hydrodeoxygenation reaction using biomass-derived furans and petroleum-derived norbornene. Hβ zeolite with Si/Al ratio of 25 exhibits the best catalytic performance (yield of 75.9 %) for Diels-Alder reaction ascribed to its large surface area and high acid concentration, especially the high ratio of Brønsted/Lewis acid sites. Kinetics research indicates that Diels-Alder reaction of norbornene and 2-methylfuran shows the apparent activation energy of 45.67 kJ/mol, much lower than that of norbornene dimerization (52.48 kJ/mol) and 2-methylfuran trimerization (63.61 kJ/mol). Importantly, the synthesized fuel has much higher density, volumetric neat heat of combustion and better cryogenic viscosity (0.943 g mL −1 , 40.39 MJ/L and 12.9 mm 2 s −1 at −40 °C) than classic JP-10 fuel (0.936 g mL −1 , 39.41 MJ/L and 18.6 mm 2 s −1 at −40 °C). This work provides an efficient and promising route to produce high-performance JP-10 fuel alternatives through co-conversion of biomass and petroleum derivatives.
研究领域:
能源化学
·
催化化学
·
生物质转化
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
·
2020
·Vol. 68
·期 6
·1192-1201
·
IF 1.638
Lingqiang Meng,Jianmin Dai,Shengzhong Rong,Hongmin Gao,Ze Zhang,Yingcong Zhang,Dong Chang,Hongzhi Pan
In our work, one-step electro-deposition method was adopted to produce polyaniline (PANI) and functional multiwalled carbon nanotubes (f-MWCNTs) films on glass carbon electrodes, and the modified electrodes were applied as an electrochemical sensor for determination of 10-hydroxycamptothecine (10-HCPT). The f-MWCNTs were handled by ultrasound processing in concentrated oxidizing acid solution, which can obtain a wonderful dissolution in water and attach new functional groups, such as –COOH and –OH. Then, aniline monomer could polymerize on the surface easily. The surface characterization was investigated using various techniques including scanning electron microscope, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction, and electro-catalytic properties were characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Under optimal conditions, the resulting of PANI/f-MWCNTs sensor showed a wide linear range (3 × 10 −9 to 7 × 10 −7 mol L −1 ) and a low detection limit (1 10 −9 mol L −1 ), which is attributing to its large special surface area and good conductivity. Moreover, the modified electrodes are convenient to fabricate, which can be used to detect 10-HCPT in urine samples successfully.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Junbo He,Menglu Wang,Peipei Zhu,Hailong Zhang,Chun Hu,Weinong Zhang
Polyglycerol-10 dialdehyde , prepared by NaIO 4 oxidation of polyglycerol-10, was used as a novel cross-linking reagent for sodium caseinate (NaCas) modification, which can alter the emulsifying properties of proteins. The results revealed that cross-linking resulted in an approximately two-fold increase in the surface hydrophobicity and a 15° increase in the contact angle of NaCas. The emulsifying activity index, emulsion stability index, and oil-binding capacity of cross-linked NaCas also increased significantly. Furthermore, the stabilization of soybean oil-in-water emulsion demonstrated that cross-linked NaCas can prevent oil droplets from aggregating during storage; meanwhile, compared to the native protein, the cross-linked NaCas improved the stability of the emulsions under high ionic strength and temperatures. It is worth noting that the polyglycerol-integrated NaCas showed less adsorption on the glass surface. These findings clearly illustrate that renewable polyglycerol can be used as a novel cross-linking reagent for protein modification, which will provide proteins with promising features for use as emulsifiers in the food, pharmaceutical, and cosmetic industries.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
·
2017
·
IF 3.756
Qin Tang,Fangling Ji,Jingyun Wang,Lianying Guo,Yachen Li,Yongming Bao
Quercetin (Qu) is known as a dietary antioxidant with numerous bioactivities, but its function in anti-cancer has not been fully investigated. Here, we show that Qu at low doses (≤ 10 μM) significantly enhances the inhibition of 10-hydroxy camptothecin (HCPT) on the proliferation of MCF7, BGC823 and HepG2 cells. A plasmid DNA relaxation assay indicates that the inhibition of HCPT on the catalytic activity of topoisomerase I (Topo I) is increased by Qu at 10 μM. Compared to the treatment by Qu or HCPT alone, phosphorylation at Ser 139 of γH2A.X in MCF7 cells starts to increase significantly (P < 0.05) at 6 h when treated by the combination of 10 μM Qu and 0.62 μM HCPT. Moreover, the combinational group successively arrests MCF7 cells at G1, S and G2/M phases from 12 h to 48 h via up-regulation of p21 and induces apoptosis at 24 h by triggering intrinsic cell death pathways. In addition, the inhibition effects of the combinational group on the proliferation of MCF7 cells are eliminated by pretreatment with 100 μM Z-VAD-FMK (a caspase inhibitor). Finally, by using nude mice xenografting assay of MCF7 cells, we demonstrate that tumor inhibition rates of combinational group are significantly higher than single-drug group. In summary, the synergic anti-cancer mechanism of Qu and HCPT in MCF7 cells is through the combined inhibitory effects of Qu and HCPT on Topo I, which synergistically induce cell cycle arrest and apoptosis by triggering DNA damage.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Huihui Li,Cailing Yang,Xinyue Zhu,Haixia Zhang
A simple ratiometric fluorescent sensor for fructose was presented. It consisted of 10-hydroxybenzo[ h ]quinoline (HBQ) which showed emission at 572 nm and 3-pyridylboronic acid (PDBA) whose complex with HBQ gave emission at 500 nm. The reaction of fructose with PDBA inhibited the complexation of HBQ with PDBA, resulting in the change of dual-emission intensity ratio. The sensor well quantified fructose in the range of 0.015–2.5 mM with detection limit of 0.005 mM. Besides, this sensor exhibited excellent selectivity and was successfully applied to fructose detection in food. This work provides a simple ratiometric sensing platform for sensitive and selective detection of fructose.
研究领域:
分析化学
·
食品科学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2020
·
IF 7.098
Weiwei Zhang,Hui-Jing Li,Liwei Chen,Jiani Sun,Xingyi Ma,Yunfei Li,Chao Liu,Xu Han,Boyi Pang,Yan-Chao Wu
An environment-friendly scaling–corrosion inhibitor composed of 10-methylacridinium iodide (MAI) and sodium citrate (SC) was developed, whose scale inhibition performance were studied through weight loss, electrochemical techniques , static tests and surface analysis techniques (SEM, SECM , XPS , XRD). The optimum mass ratio of MAI and SC corrosion inhibition was found to be 1:2, and the optimum mass ratio of scale inhibition was 1:3. The experimental results showed that MAI–SC mixture as mixed-type scaling–corrosion inhibitor with excellent corrosion ( η w = 92.7%) and scale inhibition performance ( η Ca = 98.3%). The electrochemical stability of the inhibitor film was investigated by chronoamperometry (CA). SEM and XRD simulations indicated that the MAI–SC mixture inhibitor can distort and disperse the crystal lattice of calcium carbonate without forming scale, and it plays a dominant role in the inhibition of calcite (110) surface growth. The structure-activity relationship between the molecule structure of the MAI–SC mixture and its scale–corrosion inhibition performance was discussed based on the quantum chemistry calculation.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Xiaodan Wu,Ying Wang,Bingyang Qin,Guansong Shao,Zihan Wang,Tao Wang,Yujie Fu
Targeted separation of active phytochemicals is urgently needed in the natural medicine field. In this paper, due to the natural porosity and high biocompatibility of cellulose , a nanocellulose membrane combined with surface molecular imprinting was successfully prepared; the efficient nanocellulose-based molecular imprinted membrane (NC-MIM) provided good adsorption for the targeted separation of phytochemicals such as 10-deacetylbaccatin III (10-DAB), an essential intermediate in the synthesis of the anticancer drug paclitaxel. Through a series of characterization and adsorption experiments, the adsorption mechanism of NC-MIM was determined. At pH 8.0 and temperatures of 20 °C–40 °C, the maximum capacity of NC-MIM for adsorption of 10-DAB reached 66.90 mg g − 1 , and the content of 10-DAB was dramatically increased 17.5-fold after adsorption. The specific adsorption results showed that NC-MIM had excellent capacity for targeted separation of 10-DAB from among taxane structural analogues. Even after ten cycles, NC-MIM demonstrated a remarkable adsorption capacity of 86.43%, thereby indicating exceptional selectivity and stability. The successful implementation of NC-MIM for green, safe, and efficient enrichment of phytochemicals from plants provides a promising new approach and valuable insights into its practical application.
研究领域:
植物化学
·
药物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2020
·
IF 4.613
Wei Zhang,Yang Luo,Yang Zhou,Ming Liu,Weitao Xu,Bing Bian,Zhu Tao,Xin Xiao
A new fluorescent probe composed of cucurbit[10]uril (Q[10]) and a water-soluble derivative of pyrene, aminopropyl-1-pyrenebutanamide (PBA), is designated. 1 H NMR spectroscopy reveals that PBA is encapsulated into Q[10] in an aqueous solution to form a stable 1:1 host-guest inclusion complex of PBA@Q[10]. This complex behaves as a highly selective fluorescence quenching probe for Fe 3+ in aqueous solutions that may be attributed to the efficient absorption of Fe 3+ , which hindering absorption of PBA@Q[10] probe at the excitation wavelength of 332 nm, and competitive experiments for the coexistence of Fe 3+ with one or all of the other metal ions (37 metal ions) reveals that the selectivity of PBA@Q[10] to Fe 3+ is not disturbed by the addition of 50.0 equiv. of the other metal ions. The limits of detection of Fe 3+ was determined to be 0.55 mM. Furthermore, the fluorescence enhancing effect of PBA@Q[10] for Ag + is detected at the above wavelength. According to the mechanistic studies, the fluorescence enhancing route involves interaction of Ag + ions with the probe, mainly under an internal charge transfer between Ag + and the probe. Outcomes of this study suggest that PBA@Q[10] probably functions as a potential and promising fluorescence sensor for monitoring of different polluting metal ions in waste waters.
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 88
·71749-71755
·
IF 3.84
Xueni Li,Longzhen Zheng,Yimin Wang,Nan Zhang,Yan Lou,Ting Xiao,Jiani Liu
This paper reports on an experimental research of a 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) enveloped 10-methylphenothiazine (MPT) catalyst (MPT-HP-β-CD) with high sensitivity and wonderful electrical conductivity for the electrochemical detection of reduced glutathione (GSH) in a neutral environment. We obtained a linear response range from 10−6 mol L−1 to 5.8 × 10−4 mol L−1 for GSH with a low detection limit of 2.87 × 10−7 mol L−1, providing a potentially usefulness in the development of sensors for non-enzyme detection of GSH.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）