ACS Omega
·
2024
·
IF 3.7
Tengjiao Fan,Lin Shen,Yaxin Huang,Xin Wang,Lijiao Zhao,Rugang Zhong,Peng Wang,Guohui Sun
Lung cancer ranks as the second most diagnosed cancer and the leading cause of cancer-related deaths worldwide. Novel chemotherapeutic strategies are crucial to efficiently target tumor cells while minimizing toxicity to normal cells. In this study, we proposed a combination strategy using energy blocker lonidamine (LND) and cytotoxic drug nimustine (ACNU, 1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-chloroethyl)-3-nitrosourea) to enhance the killing of a human lung cancer cell line and investigated the potential chemo-sensitizing mechanism of LND. LND was found to remarkably increase the cytotoxicity of ACNU to A549 and H1299 cells without significantly affecting normal lung BEAS2B cells. The combination of LND and ACNU also produced significant effects on cell apoptosis, colony formation, cell migration, and invasion assays compared to single drug treatment. Mechanistically, LND decreased intracellular ATP levels by inhibiting glycolysis and inducing mitochondrial dysfunction. Furthermore, the combination of LND and ACNU could intensify cellular oxidative stress, decrease cellular GSH contents, and increase reactive oxygen species (ROS) production. Notably, LND alone dramatically downregulated the expression of DNA repair protein MGMT (O6-methylguanine-DNA methyltransferase), enhancing DNA interstrand cross-link formation induced by ACNU. Overall, LND represents a potential chemo-sensitizer to enhance ACNU therapy through energy inhibition, disrupting redox homeostasis and downregulating MGMT expression in human lung cancer cell line under preclinical and clinical background.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Xueyuan Li,Xuezhong Du
Surface enhanced electrochemiluminescence (SEECL) emission of the Ru(bpy) 3 2+ (bpy = 2,2′-bipyridyl)/tripropylamine (TPA) system by [email protected] 2 core/shell nanoparticles (NPs) was achieved owing to the electromagnetic field enhancement induced by localized surface plasmon resonance (LSPR) of internal Au cores. The Ru(bpy) 3 2+ /TPA system in electrolyte solution showed a maximum ECL enhancement when a glassy carbon electrode (GCE) was modified with [email protected] 2 NPs ( [email protected] 2 /GCE; Au core, ca. 68 nm; silica shell, 12 nm). The ECL emission of the system in the case of [email protected] 2 /GCE was 29 fold stronger than the ECL emission in the case of bare GCE, ca. 39 fold stronger than the ECL emission in the case of hollow silica NP-modified GCE, and 100 fold stronger than the ECL emission in the case of AuNP-modified GCE. To the best of our knowledge, the respective folds of ECL enhancement in the Ru(bpy) 3 2+ /TPA system by the LSPR-induced electromagnetic field of AuNPs are the largest reported to date. The ECL emission of TPA •+ radical cations was detected for the first time taking the advantage of the electromagnetic field enhancement. A SEECL platform based on the [email protected] 2 /GCE in the Ru(bpy) 3 2+ /TPA system was constructed for the detection of dopamine (DA). This is the first example that the AuNP-enhanced ECL emission was quenched by DA for the selective detection of DA, and a wide linear range of 0.01–600 μM directly relevant to DA concentration was obtained with a low detection limit of 0.004 μM and high sensitivity and accuracy. The mechanism that the ECL emission of the Ru(bpy) 3 2+ /TPA system was quenched by DA for the detection of DA was clearly elaborated. The level of DA in human serum was determined using the SEECL platform.
研究领域:
分析化学
·
电化学
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Meng Li,Hongmei Yang,Chao Ma,Yan Zhang,Shenguang Ge,Jinghua Yu,Mei Yan
In this paper, a novel and sensitive signal-off electrochemiluminescence (ECL) aptasensor was proposed for the detection of adenosine triphosphate (ATP) on a nanoporous gold (NPG) modified glassy carbon electrode. The NPG was easily synthesized by a selective dissolution of silver from silver/gold alloy in nitric acid . The ECL aptasensor consisted of two main elements: an ECL substrate, made by modifying the amino group functionalized Ru(bpy) 3 2+ -doped silica (Ru-silica), and an ECL intensity switch, which contains two probes, split from one aptamer. The first probe (ssDNA1) was functionalized with a thiol group at one end and attached to Ru-silica through the linking agent of N-succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. The other one (ssDNA2) is ferrocene-labeled probe, which was functionalized with ferrocene tag at the amino group functionalized end. We demonstrated that the ssDNA1/ATP/ssDNA2 sandwich composites layer could be formed in the presence of ATP, and lead to a quenching of Ru(bpy) 3 2+ . However, only a slight decrease could be observed in the absence of ATP. Under optimal conditions, the proposed biosensor provided a wide linear response range over 0.1 pmol L −1 –10.0 nmol L −1 with a detection limit of 0.03 pmol L −1 .
研究领域:
分析化学
·
生物化学
·
纳米技术
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOPARTICLE RESEARCH
·
2022
·Vol. 24
·期 1
·1-13
·
IF 2.533
Lu Yifei,Wang Sui,Guo Zhiyong,Hu Yufang
A ratiometric fluorescence sensor (c-CDs/Ru(bpy) 3 2+ ) based on the selective recognition of chitosan carbon dots (c-CDs) on ClO − and combine with Ru(bpy) 3 2+ was established for sensitive and visual monitoring of hypochlorite ion (ClO − ). Using this nanosensor, a highly sensitive assay of ClO − in the linear range of 0.026 − 70 μM with a low detection limit (LOD) of 8.6 nM was provided by quenching the blue emission fluorescence. It is more attractive that ClO − can also be visualized by this nanosensor via evident color changes of the solution (from blue to red) under an ultraviolet (UV) lamp. In addition, since c-CDs and Ru(bpy) 3 2+ have good water dispersibility and water solubility, respectively, the sensor can be applied to the detection of HClO in 100% aqueous solution. This is a new colorimetric and fluorescent dual-mode nanosensor with more simple preparation procedures, low cost, and excellent biocompatibility. It provides a new method for detecting ClO − and promotes the development of biosensing.
研究领域:
分析化学
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纳米技术
·
生物传感器
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Jiawei Gong,Tongtong Zhang,Peng Chen,Fei Yan,Jiyang Liu
Herein we reported the construction of dual-mode electrochemiluminescence (ECL) and electrochemical (EC) sensing platform using the bipolar silica nanochannel array (bp-SNA) confined with the bifunctional probe (tris(2,2′-bipyridyl) ruthenium , Ru(bpy) 3 2+ ) inside the nanochannels (designated as Ru@bp-SNA). With the asymmetric surface charges, bp-SNA on the indium tin oxide electrode can effectively function as electrostatic nanocage for the stable confinement of Ru(bpy) 3 2+ via dual electrostatic force , namely electrostatic attraction of inner negative surface and electrostatic repulsion of outer positive surface. Ru(bpy) 3 2+ immobilized into the nanospace acts as both highly efficient ECL luminophore and well-defined EC redox molecule to generate strong signal. Since the modification of bio-recognition units on the top of Ru@bp-SNA is able to confer excellent recognitive capacity and obtain the formation of complex in the presence of specific analyte, the decrease of ECL and EC signals enables the quantitative detection of this analyte. As proof-of-concept experiments, two tumor related biomarkers, CEA and CA15–3, have been successfully determined in aqueous solution and human serum by ECL and EC modes, respectively.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物传感器
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xinxia Wang,Wei Yuan,Zhilan Sun,Fang Liu,Daoying Wang
Pseudomonas aeruginosa ( P. aeruginosa ) is an opportunistic infection-causing pathogen that threatens human health. Accordingly, a rapid and ultrasensitive analytical method is required urgently. Herein, an ultrasensitive multicolor electrochromic sensing platform was established on the basis of a closed bipolar electrode (BPE). Prussian blue (PB), the blue spot that can be easily electrodeposited, was selected as an electrochromic indicator at the closed BPE cathode. Integrating with the anodic emitter, Ru(bpy) 3 2+ , which emitted optical red light, visualized multicolor electrochromism was achieved on closed BPE. Particularly, physical separation between the positive and negative poles of the closed BPE greatly prevented mutual interference between Ru(bpy) 3 2+ and PB. Consequently, the sensitivity and accuracy of the proposed biosensor considerably improved. Notably, owing to magnetic-separation technology, the closed BPE surface required no modification. Without any complex pretreatment, the entire experiment time could be greatly shortened because the [email protected] completely captured P. aeruginosa in food matrix within only 20 min. By comparing the visual electrochromic colors, ultrasensitive screening of P. aeruginosa was accomplished within 1–10 8 CFU mL −1 . Combining the merits of closed BPE, electrochemiluminescence (ECL), and electrochromic, this strategy provided an accurate and intrinsic way for visual detection of P. aeruginosa , as well as great potential in measuring other pathogens.
研究领域:
生物技术
·
分析化学
·
微生物学
·
传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Binghui Li,Xin Wang,Xiaocui Yin,Huiting Hu,Guobing Wei,Jing Zhang,Hao Fan
A dual-mode electrochemiluminescence (ECL) and fluorescence (FL) probe was developed based on functionalized metal-organic framework nanosheets (RuMOFNSs) and (3-((2,4-dinitrophenyl)thio)phenyl)methanol (DNB) for the highly sensitive detection of hydrogen sulfide (H₂S) in homogeneous systems. Compared to conventional heterogeneous detection strategies, the homogeneous nature of this probe eliminates signal loss associated with solid-phase immobilization, thereby enhancing the interaction efficiency between the probe and target molecules. The developed sensing platform exhibited exceptional sensitivity, achieving detection limits of 0.18 pM in ECL and 0.4 nM in FL, with recovery rates ranging from 96.1 % to 101.7 %. Moreover, the probe demonstrated high selectivity against potential interfering metal ions and proved effective for monitoring food spoilage and dynamic H₂S fluctuations in rat brain tissue. Compared to existing detection approaches, this dual-mode probe offers superior sensitivity, selectivity, and broader applicability, underscoring its potential for environmental and biomedical analyse
研究领域:
分析化学
·
环境科学
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生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2019
·Vol. 23
·期 8
·2579-2588
·
IF 2.531
Du Fangkai,Zhang Hui,Tan Xuecai,Ai Chenhao,Li Mengru,Yan Jun,Liu Min,Wu Yeyu,Feng Defen,Liu Shaogang,Han Heyou
A sensitive electrochemiluminescence (ECL) aptasensor based on 3D graphene is described for thrombin detection utilizing nitrogen-doped graphene quantum dots doped silica (NGQDs@SiO 2 ) nanocomposite labeling as an enhancer. Ru(bpy) 3 2+ -Pt nanoparticles (Ru-PtNPs) with an ECL signal were dropped onto a 3D graphene-modified electrode, and then, a complementary thrombin aptamer (cDNA) was conjugated to the Ru-PtNPs. Finally, NGQDs@SiO 2 , coupled with the thrombin aptamer as the booster, was attached to the electrode surface by using hybridization with the cDNA, and the ECL intensity was efficiently enhanced. The target thrombin induced the NGQDs@SiO 2 nanocomposites labeled with the thrombin (TB) aptamer to be extracted because of their strong binding with thrombin. The immobilization amount of NGQDs@SiO 2 on the modified electrode decreased. Thus, the presence of thrombin could result in a decrease in the ECL signal. On the basis of the signal amplification factors, the fabricated ECL aptasensor demonstrated excellent properties with concentrations varying from 2.0 pM to 50 nM and a detection limit of 23.1 fM.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2021
·Vol. 50
·期 30
·10436-10445
·
IF 4.39
Cailing Jiang,Feixiang Huang,Yi Chen,Li Jiang
The homeostasis and imbalance of glutathione (GSH), an important antioxidant in organisms, are one of the key signals that reflect the health of organisms. In this paper, a novel SERS sensing platform based on Ag film@Si that self-assembled using silver nanospheres was proposed, which was used for the highly sensitive and selective detection of GSH. With the aid of an oil/water/oil three-phase system, the nano-silver film was self-assembled and finally deposited on silicon wafers. The heterobifunctional crosslinking agent N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP), which contains pyridine rings and disulfide bonds, was involved in the exchange reaction between the sulfhydryl groups and disulfide bonds. With the addition of GSH, the breakage of disulfide bonds was promoted, thereby enhancing the SERS signal of SPDP. GSH can be detected sensitively by detecting the changes in the SPDP signal. The detection limit of GSH is 10 nM, and the method is still highly stable when the external environment is serum or other more complex environments.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Jing Zhang,Dan Xu,Zijie Deng,Xueping Tan,Dongnan Guo,Yanru Qiao,You Li,Xiaofang Hou,Sicen Wang,Junbo Zhang
A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO 3-x QD S ) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy) 3 2+ were doped on silica nanoparticles (Ru@SiO 2 ), which greatly enhanced the ECL potential. AuNPs@WO 3-x QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times. Under ideal conditions, the limit of detection for thrombin in serum was determined to be 0.28 μU/mL with a linear range from 1 μU/mL to 1 U/mL. In addition, the developed ECL biosensor was used to screen for thrombin inhibitors from 12 compounds in Artemisiae Argyi Folium. Among the compounds tested, it was observed that 100 μmol/L luteolin exhibited a significantly higher inhibition rate (exceeding 80%) compared to apigenin , isorhamnetin , naringin , or eriodictyol . In an in-vitro anticoagulation experiment, luteolin (100 μmol/L) prolonged APTT by 49%, and the molecular docking assay indicated that luteolin had binding sites of Gly219 and Asp189 in the active pockets of thrombin. This may have been the main reason underpinning luteolin's anticoagulation effects. Overall, the Ru@WO 3-x QD S ECL biosensor provided a reliable strategy for thrombin activity assay and screening of anticoagulant agents.
研究领域:
分析化学
·
生物化学
·
药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Tiantian Wang,Jianhong Wei,Furong Shi,Hejie Wang,Jinye He,Xudong Lv,Yuan Zhou,Pengzhi Guo,Chenglong Wang,Yangjun Xia
In recent years, polymerized small molecule acceptors (PSMAs) have emerged as a promising strategy that combines the strong absorption of small molecules with the film-forming ability and stability of polymers, thereby greatly boosting the performance of all-polymer solar cells (all-PSCs). We designed a non-fused acceptor, DTBT-IC, and its polymeric counterpart, PDTBT-Br-T, by selecting DTBT as the core, bithiophene as the π-bridge, and IC as the terminal group. PDTBT-Br-T was synthesized via Stille coupling and used with PM6 as the donor to fabricate organic solar cells. The PDTBT-Br-T-based device delivered superior performance, with a VOC of 1.050 V, JSC of 9.32 mA cm−2, FF of 45.33%, and a PCE of 4.44%, outperforming the DTBT-IC-based counterpart. Morphological and structural analyses revealed that PDTBT-Br-T exhibits more ordered backbone stacking and defined phase separation, enhancing exciton dissociation and charge transport, and suppressing energy loss. The study highlights that polymerization of DTBT-IC enhances intermolecular packing and microstructure, offering critical design insights for efficient all-PSCs.
研究领域:
材料科学
·
高分子化学
·
有机电子学
·
光伏技术
✓ 已验证
DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
·
2023
·
IF 3.6
Shuo Li,Xian Xu,Qianlin Sun,Xin Xu
This work reports an intramolecular C(sp3)–H annulation of functionalized 2,6-lutidines catalyzed by an organocalcium hydride [{(DIPPnacnac)CaH(thf)}2] (DIPPnacnac = CH{(CMe)(2,6-iPr2–C6H3N)}2). This reaction constitutes a streamlined approach for producing a new family of tetrahydro-1,5-naphthyridines and hexahydropyrido[3,2-b]azocines derivatives in good to excellent yields with high atom efficiency and broad substrates scope. A calcium alkyl complex was isolated from the stoichiometric reaction between calcium hydride and the substrate through deprotonation, which was structurally characterized and confirmed as the catalytic intermediate.
研究领域:
有机化学
·
催化
·
合成化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）