MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 11
·1-9
·
IF 5.479
Xue Jin-Hua,Liu Ling,Wang Yong-Sheng,Li Jia-Qian,Li Mei,Qu Yue-Ning,Li Le
This work describes a method for the determination of 1-hydroxypyrene (OH-Py) via aggregation-induced quenching of the emission of protamine-coated gold nanoclusters using 9-hydroxyphenanthrene (OH-Phe) as a sensitizer to boost the emission efficiency of nanoprobe. Under optimum conditions, the drop in fluorescence intensity at excitation/emission wavelengths of 300/596 nm is proportional to the concentrations of OH-Py in the range from 1.0 to 65 nM. The relative standard deviations are 4.2, 2.4 and 1.9% (for n = 11) at concentration levels of 8.0, 32 and 48 nM of OH-Py, respectively. The detection limit is 0.3 nM which is much lower than that of some previously reported methods. The recoveries from urine samples spiked with OH-Py ranged between 94.4 and 98.8%.
1-Hydroxypyrene (OH-Py) can trigger the aggregation of protamine-gold nanoclusters (PRT-AuNCs), resulting in the emission quenching of PRT-AuNCs. 9-Hydroxyphenanthrene (OH-Phe) can boost the emission efficiency of nanoprobe. Thereby, a highly sensitive assay of OH-Py was established.
研究领域:
分析化学
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环境医学
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纳米生物学
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2020
·
IF 4.389
Jin-Hua Xue,Ke-Ping Xiao,Yong-Sheng Wang,Ling Liu,Jia-Qian Li,Mei Li,Yue-Ning Qu,Xi-Lin Xiao
In this study, we confirmed that protamine-templated gold nanoclusters (PRT-AuNCs) exhibit aggregation-induced emission properties (AIE-PRT-AuNCs). 1-Hydroxypyrene (1−OHPy) further induced the aggregation of AIE-PRT-AuNCs via hydrogen bonding and electrostatic and hydrophobic interactions, resulting in the aggregation-induced photoluminescence enhancement of AIE-PRT-AuNCs. 9-Hydroxyphenanthrene was able to decrease the background signal, thus increasing the sensitivity of the method. Based on these findings, a cost-effective, highly sensitive and selective strategy was proposed for the quantitative detection of 1−OHPy. This method displayed a wide linear range of 0.924 – 74.1 nmol/L with a low detection limit of 0.277 nmol/L, showing great potential for the monitoring of 1−OHPy in human urine. This strategy may provide a theoretical basis for future studies of the AIE properties of metal nanoclusters and their applications in the field of chemical and biological sensing.
研究领域:
分析化学
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生物医学工程
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环境科学
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DOI
PubMed
高影响因子
Alexandria Engineering Journal
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2025
·
IF 6.8
Yinan Hu,Tao Liu,Bo Guo,Miao Ni,Yanrong Li
This study presents a novel surface-enhanced Raman spectroscopy (SERS) method for detecting CA19–9 in colorectal adenocarcinoma using 4-thiobenzoic acid (4-TBA) functionalized gold nanoparticles (nanospheres). The optimized SERS probes exhibited a maximum enhancement at pH 7 and 50 μM 4-TBA concentration, with stable signal achieved after 2 hours of incubation. The assay demonstrated a wide dynamic range of 1–100 U/mL, with a lower limit of quantification of 1.0 U/mL. Excellent selectivity was observed against common serum interferents, with normalized SERS intensities within 10 % of CA19–9 alone. Recovery studies in spiked serum samples yielded results ranging from 94.5 % to 105.2 %, indicating minimal matrix effects. Clinical validation using 70 serum samples (50 colorectal adenocarcinoma patients, 20 healthy volunteers) showed strong agreement with a commercial ELISA kit (Pearson's r = 0.987, p < 0.0001). Bland-Altman analysis revealed a mean bias of −0.37 U/mL between SERS and ELISA methods. The SERS-based approach offers advantages including rapid analysis (<1 hour), small sample volume requirements (5–10 μL), and potential for multiplexing. This method shows promise for early detection, disease monitoring, and treatment response evaluation in colorectal adenocarcinoma, with potential applications in both clinical and research settings.
研究领域:
肿瘤学
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医学诊断
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纳米技术
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光谱学
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产品关联
DOI
高影响因子
Polymer Chemistry
·
2020
·Vol. 11
·期 37
·5992-5997
·
IF 5.342
Haipeng Lu,Xiang Wang,Xianjing Zhou,Wei Zhang,Xinping Wang
Over the past few decades, global paper consumption has continued to increase. The growing demand for paper has aggravated the negative impact on the environment. Therefore, the development of an erasable ink or rewritable paper seems to be an ideal way to alleviate this crisis. In this work, a hydrophilic anthracene derivative with nucleophilic substitution at position 9 was designed and synthesized. Such an anthracene derivative could disperse in aqueous solution and dimerize fast under irradiation of UV or visible light, accompanied by fluorescence quenching. Its aqueous solution could be used as a novel ink to be written on conventional paper, of which the handwriting was only visible under ultraviolet light. When exposed to sunlight, the handwriting would gradually disappear and not reappear after irradiating with ultraviolet light or heating. Therefore, it can be used as an erasable ink for applications in data confidentiality or paper reuse.
研究领域:
材料科学
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环境科学
·
化学
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DOI
高影响因子
CHINESE CHEMICAL LETTERS
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2014
·
IF 1.178
Xiao-Lan Li,Dong-Ling Meng,Jiao Zhao,Ya-Ling Yang
This study aimed to establish a rapid analytical method to determine antioxidants in essence. A simple, efficient and practical, vortex-assisted, cloud-point extraction (VACPE) procedure is proposed for extracting and pre-concentrating four different of synthetic phenolic antioxidants (SPAs), propyl gallate (PG), tert -butylhydroquinone (TBHQ), butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) in essence prior to high performance liquid chromatography (HPLC) analysis. The non-ionic surfactant, fatty alcohol polyoxyethylene ether-9 (AEO-9), was used as extractant and vortex-mixing was utilized to reduce extraction time and improve extraction efficiency. The effective parameters of the extraction process, such as volume of extraction solvent, pH, vortex-mixing time, equilibration temperature and time, were optimized. Under the optimum conditions, the linear range of PG, TBHQ, BHA and BHT was 8.0–800 ng/mL. All correlation coefficients of the calibration curves were higher than 0.996 and relative standard deviations (RSD, n = 5) were 2.36%–5.46%. The proposed method was successfully applied to the extraction and determination of antioxidants in essence samples with satisfactory relative recoveries of 89.4%–103.5%. The results confirmed the SPAs of essence could be effectively monitored by this method and also established good reference criteria for essence.
研究领域:
分析化学
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食品科学
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DOI
高影响因子
ANALYST
·
2022
·Vol. 148
·期 4
·752-761
·
IF 5.227
Xintong Lu,Luoyu Ding,Haohan Song,Wenxin Yu,Chaoqing Dong,Jicun Ren
Matrix metalloproteinase-9 (MMP-9) plays an important role in tumor progression. It is of great significance to establish a sensitive in situ assay strategy for MMP-9 activity in single living cells. Here a novel in situ single molecule spectroscopy method based on the fluorescence correlation spectroscopy (FCS) technique was proposed for measuring the MMP-9 activity at different locations within single living cells, using a fluorescent specific peptide and a reference dye as dual probes. The measurement principle is based on the decrease of the ratiometric translational diffusion time of dual probes in the detection volume due to the peptide cleavage caused by MMP-9. The peptide probe was designed to be composed of an MMP-9 cleavage and cell-penetrating peptide sequence that was labeled with a fluorophore and conjugated with a streptavidin (SAV) molecule. The ratiometric translational diffusion time was used as the measurement parameter to eliminate the effect of intracellular uncertain viscosity. The linear relationship between the ratiometric diffusion time and MMP-9 activity was established, and applied to the determination of enzymatic activity in cell lysates as well as the evaluation of the inhibitory effects of different inhibitors on MMP-9. More importantly, the method was successfully used to dynamically determine MMP-9 activity in single living cells or under the stimulation with phorbol 12-myristate 13-acetate (PMA) and inhibitors.
研究领域:
生物化学
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分子生物学
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肿瘤学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 6.735
Yuan Meng,Jianguo Jiang,Yuchen Gao,Aikelaimu Aihemaiti,Tongyao Ju,Yiwen Xu,Nuo Liu
Biogas upgrading technology is important for increasing the heating value of biogas via separating CH 4 and CO 2 , before biogas can be utilized as a vehicle fuel or for grid injection. In this work, using a porous polymeric resin (NKA-9), we prepared a new sorbent, namely polyethyleneimine (PEI)-impregnated NKA-9, for use in biogas upgrading to methane via CO 2 sorption firstly. The adsorption performance, regeneration behavior, cyclic stability and CH 4 /CO 2 separation performance were then evaluated in detail. The sorbent exhibited high adsorption capacity of up to 151.1 mg/g with negligible CH 4 adsorbed, demonstrating its high selectivity towards CO 2 over CH 4 . In column breakthrough experiments, binary mixtures were separated effectively under each set of reaction condition, where high CH 4 purities of >95% could be obtained as the final product in the outlet gas. As tested, excessive PEI loading, high temperature and low CO 2 concentration in biogas would result in unfavorable effects on biogas upgrading. In addition, we tested the regenerability of the sorbent using the two most common methods-N 2 and CO 2 purging, where the sorbent could be regenerated completely at temperatures above 85 °C and 155 °C, respectively. During the long-term operation, PEI-impregnated NKA-9 exhibited excellent cyclic stability, with 1.4% and 5.2% decreases in adsorption capacity after 30 adsorption-regeneration cycles. Detailed characterization revealed that the sorbent was chemically stable at 155 °C, in contrast to other conventional amine-modified sorbents. Therefore, the regenerable PEI-impregnated NKA-9 sorbent could be a promising choice for biogas upgrading to methane via CO 2 sorption.
研究领域:
环境科学
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化学工程
·
能源技术
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Hongzhan Liu,Fangshuo Liu,Kaixiang Ji,Yuriy T. Zholudov,Islam M. Mostafa,Baohua Lou,Wei Zhang,Guobao Xu
9-Mesityl-10-methylacridinium ion (Acr+-Mes) is a donor–acceptor molecule with a much longer lifetime and a higher energy electron transfer excited state than natural photosynthetic reaction centers. Unlike lucigenin with a coplanar geometry, Acr+-Mes has an orthogonal geometry. There is no π conjugation between Acr+ and Mes. Due to its special electron donor–acceptor structure, it does not rely on strong alkalinity to generate an electron transfer state like lucigenin, which makes it possible to achieve chemiluminescence (CL) under weakly alkaline or neutral conditions. In this study, we report Acr+-Mes CL for the first time. Acr+-Mes generates about 400 times stronger CL intensity than lucigenin under neutral conditions (pH = 7) using KHSO5 as the coreactant. Moreover, Co2+ can enhance Acr+-Mes/KHSO5 CL remarkably. Acr+-Mes/KHSO5 CL enables Co2+ detection with a linear range of 0.5–500 nM and a limit of detection of 28 pM (S/N = 3). This method was tested for the detection of Co2+ in lake water, and the standard recovery rate of 96.8–107% was achieved. This study provides a new way to develop efficient CL systems in neutral solutions.
研究领域:
分析化学
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环境科学
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材料科学
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产品关联
DOI
PubMed
高影响因子
CERAMICS INTERNATIONAL
·
2019
·
IF 3.45
Chunfang Xing,Yunhui Zhang,Bingjing Tao,Haitao Wu,YuanYuan Zhou
Low-firing La 2 Zr 3 (MoO 4 ) 9 (LZM) ceramics were prepared using the reaction-sintering (RS) route for the first time. The crystal structure, sintering characteristics, microstructure and microwave dielectric properties of LZM samples were explored scientifically. A single phase, with trigonal structure in R-3c space group, was formed at 600–800 °C. Dense sample with high relative density (>92 %) and homogeneous microstructure was obtained at 650 °C. Subsequently, EDS analysis revealed that the composition was corresponding to theoretical stoichiometric ratio of chemical formula. The chemical bonds theory of complex crystals and far-infrared spectra analysis were employed to investigate the structure-property relations of LZM samples in detail. Optimum microwave dielectric properties with a dielectric constant ( ε r ) of 10.17, a quality factor ( Q·f ) of 51,578 GHz and a temperature coefficient of resonant frequency ( τ f ) of -29.55 ppm/°C were achieved in LZM sample prepared by the RS process sintered at 650 °C.
研究领域:
材料科学
·
陶瓷
·
微波工程
·
固体化学
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产品关联
DOI
高影响因子
Pharmaceuticals
·
2025
·Vol. 18
·期 11
·1751
·
IF 4.8
Meilin Zheng,Kangyang Gao,Yirui Hong,Jingyang Le,Jingjing Cai,Hongze Liang,Wei Cui
Background:The impairment of neurite outgrowth is an early pathological hallmark underlying various neurodegenerative disorders. The promotion of neurite outgrowth was considered as a feasible strategy to treat neurodegenerative disorders. 9-Methylfascaplysin (9-MF), a marine-derived, bioactive compound, has exhibited multiple neuroprotective activities.Methods and Result:In this study, 9-MF at nanomolar concentrations promoted neurite outgrowth, upregulated the expression of growth-associated protein-43 (GAP-43), and increased the mitochondrial positive area with similar efficacy as retinoic acid in PC12 cells. 9-MF-associated differentiated expressed genes were enriched in mitochondria and synapse, forming a Rho-associated coiled-coil containing a protein kinase 2 (ROCK2)-centralized network. CMap analysis further identified positive connections between 9-MF-induced perturbation and perturbations caused by the inhibition of the ROCK2 pathway. Molecular docking analysis demonstrated a high binding affinity between 9-MF and ROCK2, indicating that 9-MF could inhibit ROCK2. Furthermore, 9-MF significantly reduced the phosphorylation of ROCK2 with a similar efficacy as fasudil, a ROCK2 inhibitor. Narciclasine, a known ROCK2 activator, almost completely abolished the effects of 9-MF on the induction of neurite outgrowth in PC12 cells.Conclusions:9-MF effectively promoted neurite outgrowth possibly via the inhibition of ROCK2, providing supporting evidence that 9-MF might be developed as a novel neurological drug.
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2022
·Vol. 10
·期 18
·7049-7057
·
IF 8.067
Yingyuan Chen,Feilong Liu,Ziwang Zhang,Junyu Hong,Maxim S. Molokeev,Ivan A. Bobrikov,Jianxin Shi,Jianbang Zhou,Mingmei Wu
Red-light-emitting materials, as pivotal components of warm white light-emitting diodes (WLEDs), have drawn increasing public focus. Among these, Mn4+-doped red light-emitting fluorides have drawn considerable attention when combined with an InGaN chip; however, they suffer from poor water stability under humid conditions. In this work, a novel fluoride red phosphor, Cs30(Nb2O2F9)9(OH)3·H2O:xMn4+ (CNOFM), with good water resistance was synthesized for the first time using a facile co-precipitation method at ambient temperature. Experiments were implemented for the precise analysis of its crystal structure, optical properties, micro-morphology, thermal behavior, and waterproof properties. 6.66% Mn4+-doped CNOFM maintained a stable crystal structure and possessed strong PL intensity located at 633 nm with high color purity of 96%. CNOFM showed better thermal and waterproof stability compared with the commercial K2SiF6:Mn4+ red phosphor. Without any surface modifications, the PL intensity remained at about 83% of the initial value after immersion in water for 60 min, and the mechanism was investigated. Finally, a warm WLED with a CRI of 92.3 and CCT of 3271 K was fabricated using the CNOFM red phosphor.
研究领域:
材料科学
·
光学工程
✓ 已验证
DOI
高影响因子
ChemCatChem
·
2016
·Vol. 8
·期 6
·1199-1207
·
IF 4.724
Lan-Lan Lou,Shanshan Li,Huanling Du,Jiong Zhang,Wenjun Yu,Kai Yu,Shuangxi Liu
Graphical Short support : A short-mesochannel SBA-15 material is applied to the immobilization of a chiral 9-amino epicinchonine iridium catalyst, which exhibit excellent catalytic activity and notably improved enantioselectivity for the asymmetric transfer hydrogenation of aromatic ketones. A short-mesochannel SBA-15 material functionalized with propylthiol groups was prepared by co-condensation and applied to the immobilization of chiral 9-amino epicinchonine. After complexing with [Ir(cod)Cl] 2 (cod=1,5-cyclooctadiene), these mesoporous materials were evaluated as catalysts for the asymmetric transfer hydrogenation of aromatic ketones. Higher enantioselectivities and comparable, or even higher, catalytic activities were achieved compared with the free catalyst. Both the short mesochannel and co-condensation approach for short-mesochannel SBA-15 materials functionalized with propylthiol groups, [SSBA-SH( x )] materials were demonstrated to contribute to the excellent catalytic performance. In addition, the catalyst SSBA-AEC(5)/Ir (AEC=9-amino epicinchonine), with low 3-mercaptopropyltrimethoxysilane (MPTMS) content, showed the best catalytic performance; a high enantiomeric excess of 84 % (homogeneous ee =60 %) along with a conversion of 97 % was achieved within 1 h for asymmetric transfer hydrogenation of acetophenone. Moreover, these immobilized catalysts showed high stability during the reaction and could be recovered for reuse.
研究领域:
材料化学
·
催化化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）