BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Hao Wu,Kai Zhang,Yaling Liu,Hongyong Wang,Jun Wu,Feifan Zhu,Pei Zou
In this work, a new binding-induced and label-free colorimetric method for protein detection has been developed on the basis of an autonomous assembly of hemin/G-quadruplex DNAzyme amplification strategy. The system consists of two proximity probes carrying two aptamer sequences as recognition elements for target, and two hairpin structures include three-fourths and one-fourth of the G-quadruplex sequences in inactive configuration as functional elements. In the presence of target protein, two proximity probes bind to the protein simultaneously, forming a stable DNA–protein complex. Then the complex triggers an autonomous cross-opening of the two functional hairpin structures, leading to the formation of numerous hemin/G-quadruplex DNAzymes. The resulting DNAzymes catalyze the oxidation of colorless 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS 2− ) to the green-colored ABTS •− with the presence of H 2 O 2 , thus providing the amplified colorimetric detection of target. Using human α-thrombin as the protein target, this binding-induced DNAzyme amplification colorimetric method affords high sensitivity with a detection limit of 1.9 pM. Furthermore, this method might be further extended to sensitive detection of other proteins by simply replacing recognition elements of proximity probes.
研究领域:
生物化学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 3
·e70125
·
IF 3.4
Guorui Yang,Jingru Zhao,Yaping Li,Mengqi Teng,Shaoxuan Yu,Yuanda Song,Haifang Xiao
Raspberry possesses several health benefits such as antitumor, antioxidant, and blood sugar-regulating activities. In order to improve the use value of raspberries, the optimal preparation process of raspberry chewable tablets (RCT) and its physical, chemical, antioxidant, and hypoglycemic properties were investigated in this research. The optimum formulation of RCT was determined as 39.1% raspberry powder, 13.1% red beet root powder, 9.8% corn starch, 15% microcrystalline cellulose, 14% mannitol, 1% magnesium stearate, and 8% ethanol (75%) by single-factor experiment and response surface methodology using sensory evaluation score and hardness as parameters. RCT prepared using the optimal formulation had a hardness of 47.33 ± 2.31 N, brittleness of 0.33%, weight of 1.00 ± 0.03 g, and a color difference Δ E = 0.427. The total phenolic and total flavonoid contents of RCT were 18.610 ± 0.483 mg/g and 3.885 ± 0.061 mg/g, respectively. Meanwhile, seven phenolic compounds in RCT were determined by high-performance liquid chromatography. Free radical scavenging abilities ((1,1-diphenyl-2-picrylhydrazyl, DPPH), (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS), and ·OH), ferric ion-reducing antioxidant power and cell tests proved the significant antioxidant ability of RCT. The obvious inhibitory activities of RCT on α-glucosidase and α-amylase reflected its hypoglycemic potential. This study provided an important basis for the development of raspberry functional foods with antioxidant and blood glucose regulating potential.
研究领域:
食品科学
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营养学
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药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2019
·Vol. 96
·期 11
·1275-1289
·
IF 1.72
Weifang Wang,Sai Han,Zhen Jiao,Jiangrui Cheng,Junying Song
In this study, Camellia oil is co-extracted from Camellia oleifera seeds and green tea scraps by supercritical carbon dioxide (SC-CO 2 ), which is optimized on the extraction yield, ABTS-scavenging activity, and total polyphenols content (TPC) of oil by single-factor experiments combined with response surface methodology (RSM). The extraction temperature, pressure, dynamic time, carbon dioxide (CO 2 ) flow rate, and seed mass ratio were investigated with single-factor experiments. The results indicated the optimum CO 2 flow rate and dynamic extraction time were 15 L hour −1 and 60 min (i.e., 2.382 kg CO 2 /100 g sample). Furthermore, the complicated effects of extraction temperature (40–50 °C), pressure (20–30 MPa), and seed mass ratio (0.25–0.75) were optimized by RSM based on the Box–Behnken design (BBD). The models with high R -squared values were obtained and used to predict the optimum operating conditions of the process. Under the optimum operating conditions (i.e., temperature of 46 °C, pressure of 30 MPa, and seed mass ratio of 0.35), the extraction yield, ABTS-scavenging activity, and TPC of oil were 14.43 ± 0.17 g/100 g sample, 73.70 ± 0.34%, and 2.18 ± 0.05 mg GAE/g oil, which were in good agreement with the predicted values. In addition, the experiments indicated that the Camellia oil obtained was rich in polyphenols, resulting in better oxidation stability and antioxidant activity than the original oil.
研究领域:
食品科学
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化学工程
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植物化学
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产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Yingnan Liu,Fei Tong,Yingran Xu,Yunyun Hu,Wenya Liu,Zan Yang,Zhenyu Yu,Guoyuan Xiong,Yibin Zhou,Yaqing Xiao
BACKGROUND Active and smart food packaging based on natural polymers and pH-sensitive dyes as indicators has attracted widespread attention. In the present study, an antioxidant and amine-response color indicator film was developed by incorporating bilirubin (BIL) into the κ-carrageenan (Carr) matrix. RESULTS It was found that the introduction of BIL had no effect on the crystal/chemical structure, water sensitivity and mechanical performance of the Carr-based films. However, the barrier properties to light and the thermal stability were significantly improved after the addition BIL. The Carr/BIL composite films exhibited excellent 1,1-diphenyl-2-picryl-hydrazyl (i.e. DPPH)/2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (i.e. ABTS) free radical scavenging abilities and color responsiveness to different concentrations of ammonia. The application assay reflected that the Carr/BIL 0.0075 film was effective in delaying the oxidative deterioration of shrimp during storage and realizing the color response of its freshness through the change of b * value. CONCLUSION Active and smart packaging films were successfully prepared by incorporating different contents of BIL into the Carr matrix. The present study helps to further encourage the design and development of a multi-functional packaging material. © 2023 Society of Chemical Industry.
研究领域:
食品科学
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材料科学
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化学
·
包装工程
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产品关联
DOI
PubMed
高影响因子
Food Science and Human Wellness
·
2020
·
IF 2.455
Ruichang Gao,Wanghui Shu,Yang Shen,Quancai Sun,Wengang Jin,Dajing Li,Ying Li,Li Yuan
Previous studies have suggested that polypeptides extracted from milk, soybean, fish, eggs, and meat possess potential anti-inflammatory effects. To date, few studies have reported the anti-inflammatory function of sturgeon peptides and their underlying mechanisms are unknown. The current study was therefore to determine the anti-inflammatory potential of sturgeon peptides with lipopolysaccharide (LPS)-induced RAW264.7 inflammatory model. Pepsin hydrolysate (PeH) was purified by ultrafiltration and Sephadex G-15 gel filtration chromatography. PeH significantly reduced the inflammatory mediator (NO) and inflammatory cytokines (IL-6、TNF-α and IL-1β) expression in a dose-dependent manner. Moreover, the purified sturgeon peptide (F2) possessed strong antioxidant potential and effectively inhibited DPPH and ABTS free radicals. F2 significantly suppressed the expression of MAPKs, IκBα, and NF-κB p65, indicating that F2 exerted anti-inflammatory influence by the inhibition of MAPK and NF-κB pathways.
研究领域:
免疫学
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生物化学
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药理学
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产品关联
DOI
高影响因子
Phytochemistry Letters
·
2014
·
IF 1.542
Guoyong Luo,Min Zhou,Yan Liu,Qi Ye,Jian Gu,Tianfang Huang,Guolin Zhang,Yinggang Luo
Two new 3-formyl-2-arylbenzofurans, iteafuranals A and B, were isolated from the 95% EtOH extract of the aerial parts of Itea ilicifolia . Their structures were elucidated as ( E )-2-(3,4-dihydroxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 1 , iteafuranal A) and ( E )-2-(3-hydroxy-4-methoxyphenyl)-3-formyl-4-hydroxy-5-(prop-1-en-1-yl)benzofuran ( 2 , iteafuranal B) by comprehensive analyses of spectroscopic data including 1- and 2-D NMR, UV, IR, and HRMS data. The structure of 1 was confirmed by X-ray crystallography analysis. Compound 1 showed DPPH free radical scavenging capacity with an IC 50 value of 0.34 mg/mL. Compounds 1 and 2 showed ABTS cation radical scavenging capacity with IC 50 values of 0.23 and 0.30 mg/mL, respectively.
研究领域:
植物化学
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药理学
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天然产物研究
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DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2018
·
IF 4.61
Meihua Fu,Jinfeng Xing,Zhiqiang Ge
Bisphenol A (BPA) has been identified as one of the endocrine disrupting chemicals. However, the issue that BPA widely exists in various environments has puzzled people for decades. To develop highly efficient, easy separation, recyclable and reusable materials for BPA degradation in water, laccase-loaded magnetic nanoflowers (MNFs) were prepared by attaching amino-functionalized magnetic nanoparticles onto the laccase-inorganic hybrid nanoflowers. Characterization results showed that MNFs were spherical, porous and hierarchical structure with an average diameter of 15 μm to which magnetic nanoparticles was successfully attached through electrostatic force. MNFs exhibited excellent catalytic activity on BPA degradation under room temperature in the presence of ABTS. Under optimized conditions, MNFs reached 100% BPA degradation for only 5 min. In addition, it still retained over 92% of its initial activity after 60 days of storage at 4 °C, indicating that its thermal and storage stabilities have been improved. When the MNFs was recycled and reused 5 cycles, only 5% decrease in degradation efficiency of BPA was observed. These results suggest that MNFs possess great efficiency and reusability in the treatment of aqueous solution containing BPA and is a novel promising material.
研究领域:
环境科学
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材料科学
·
化学工程
·
生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Xiaohan Wang,Bing Li,Shanshan Sun,Qingqing Liu,Jin Zhu,Xian Zhou,Huijin Zhang,Qian Wu,Liangsheng Wang
Antique Lotus ( Nelumbo ) is a perennial aquatic plant with unique historical significance and cultural value, whereas its potential economic value hasn’t been fully explored. The present study showed that lotus seedpods had significantly higher antioxidant capacity than other parts by FRAP, ABTS, and ORAC assays and analyzed the proanthocyanidins and flavonols in the seedpods of Antique Lotus. Polyphenols contributed to great antioxidant activity and 51 polyphenols were identified by UPLC-TQ-MS analysis. In which, 27 compounds were identified from lotus seedpods for the first time, including 20 trimers, 5 dimers and 2 tetramers of proanthocyanidin. Total proanthocyanidins explained 70%–90% of the different antioxidant activities and the content of proanthocyanidin trimers showed the strongest correlations with the antioxidant activities. This study provided a fundamental reference for the research of polyphenols in lotus and found that Antique Lotus seedpod extracts have the promising prospects of additives used in feed and food processing.
研究领域:
植物学
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植物化学
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食品科学
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农业化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Kun Wang,Mengmeng Niu,Di Yao,Jing Zhao,Yue Wu,Baoxin Lu,Xiqun Zheng
A cell-bound exopolysaccharide (c-EPS) from Lactobacillus fermentum S1 was isolated and purified to near homogeneity by anion exchange and gel filtration chromatography. The c-EPS is a homogeneous heteropolysaccharide with an average molecular weight of 7.19 × 10 5 Da and comprises mainly mannose, rhamnose, glucose, and galactose. Fourier transform infrared spectroscopy spectrum of the c-EPS exhibited typical characteristic absorption peaks of polysaccharides. Methylation and NMR analyses showed that the c-EPS had a backbone of α-D-Gal p -(1 → 3), α-L-Rha p -(1 → 2), α-D-Glc p -(1 → 3), β-D-Gal p -(1 → 3), β-D-Glcl p -(1 → 2), and β-L-Rha p -(1 → 3,4) residues, terminated with α-D-Man p -(1 → residue. The advanced structure study indicated the c-EPS not to have a triple-helical conformation, while the microstructural study revealed a hollow porous structure for c-EPS. Further, the thermal analysis showed that the degradation temperature for the c-EPS was 288.0 °C; its peak temperature was 89.4 °C with an enthalpy value of 273.1 J/g. Moreover, the c-EPS exhibited potent DPPH, hydroxyl, and ABTS + radicals scavenging activities, as well as FRAP in a dose-dependent manner, which could significantly enhance the T-AOC and SOD activity and reduce MDA level in Caenorhabditis elegans. Therefore, this c-EPS could be utilized as a promising natural antioxidant for application in functional foods.
研究领域:
食品科学
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生物化学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Journal of Functional Foods
·
2015
·
IF 3.574
Guan-Lin Chen,Song-Gen Chen,Ying-Qing Xie,Fu Chen,Ying-Ying Zhao,Chun-Xia Luo,Yong-Qing Gao
In order to provide new information on the antioxidant activities before and after in vitro digestion of the selected flowers for consumers, nutritionists, and food policy makers, total phenolic and flavonoid contents and antioxidant capacities of 23 edible flowers were evaluated. In addition, some bioactive components in the flowers were also identified and quantified by ultra performance liquid chromatography (UPLC). The correlations between 2, 2-diphenyl-1-picrylhydrazylradical scavenging activity assay (DPPH), Ferric reducing antioxidant power assay (FRAP), and ABTS •+ radical cation scavenging activity assay (ABTS) values and total flavonoid contents and total phenolic contents were also evaluated. Three flowers, Osmanthus fragrans (Thunb.) Lour, Paeonia lactiflora Pall, and Rosa rugosa Thunb (purple) showed the strongest antioxidant activities before or after the digestion, which implied that these flowers are important natural sources for the prevention of oxidative stress diseases.
研究领域:
食品科学
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营养学
·
药理学
·
植物科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Yidong Xiao,Qilin Huang,Zhaomin Zheng,Han Guan,Shiyu Liu
A Cordyceps sinensis exopolysaccharide (EPS)-conjugated selenium nanoparticles (SeNPs) were successfully constructed through the reduction of SeO 3 2− . The EPS-SeNPs were characterized in terms of formation, morphology, size, Se distribution and phase by UV–vis, FT-IR, transmission electron microscopy (TEM), dynamic light scattering (DLS), energy dispersive X-ray (EDX) and wide angle X-ray diffraction (WAXD) measurements. Results revealed that the SeNPs conjugated to EPS were amorphous and could be well dispersed at a size range of 80–125 nm. The interactions between the OH groups of EPS and SeNPs substituted for intermolecular interaction in native EPS to form new C O⋯Se bonds, resulting in good dispersion of SeNPs in the EPS matrix. Besides, the EPS-SeNPs at different Se/P ratios exhibited significant scavenging ability on superoxide anion radical (O 2 − ) and ABTS radical cation (ABTS + ) when compared to pure EPS, indicating that the conjugated SeNPs reinforced antioxidant effect of EPS. This work not only provides a simple and efficient way to construct well-dispersed SeNPs in aqueous system, and demonstrates the vital role of the EPS as a biopolymer template for dispersion, stabilization and size control of SeNPs, but also finds the EPS-SeNPs can potentially serve as a good antioxidant towards O 2 − and ABTS + .
研究领域:
生物化学
·
纳米生物学
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食品科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Lijun Xu,Dongguo Xue,Jialin Sai,Lu Zhou,Renjun Pei,Aihua Liu
Although enzyme mimics have been widely developed, limited catalytic efficiency is still a bottleneck, especially under neutral condition. Herein, we reported the bioactive quinaldic acid (QA) significantly boosted the peroxidase-like activity of Co 2+ in the presence of bicarbonate (HCO 3 − ). With 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonate) (ABTS) as the substrate, the catalytic activity of Co 2+ (1 μM) was increased by over 300 times upon adding 100 μM QA. The formed Co 2+ complex had much higher turnover number (5.52 min −1 ) than that of cobalt-based nanozymes (0.011–0.51 min −1 ) in decomposing H 2 O 2 . Based on this system, ultrasensitive colorimetric methods for the detection of Co 2+ , bicarbonate and urease activity were achieved with limits of detection of 4.6 nM, 40 μM and 0.00125 U/mL, respectively. For the first time, this work established an ultrasensitive method for the detection of urease activity by activating a peroxidase-like mimic with the produced HCO 3 − .
研究领域:
分析化学
·
生物化学
·
催化
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）