ELECTROCHIMICA ACTA
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2020
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IF 6.215
Mengjie Zhang,Zixuan Chen,Haixin Qin,Xiaoyan Yang,Wei Cao,Shufeng Liu
Abtract Heme binds to amyloid β -peptide (A β ) in the brain of Alzheimer΄s disease (AD) patients, thus forming heme-A β complexes and leading the characteristic pathological features of AD. The heme-A β complexes reduce O 2 by 2e − to obtain co-reactant H 2 O 2 . In this work, two-dimensional graphite-like carbon nitride (g-C 3 N 4 ) was hybridized with heme via π-π interaction to construct an electrochemiluminescence (ECL) biosensor for detection of A β peptides. The capture aptamer was firstly immobilized on the gold electrode, and then incubated with target A β peptides and g-C 3 N 4 -heme. The g-C 3 N 4 -heme-A β composite was subsequently anchored on the electrode and showed a signal-on, label-free ECL emission based on dual co-reactants enhancement attributed to in-situ generated H 2 O 2 and original K 2 S 2 O 8 in PBS buffer. Under the optimum experimental conditions, the ECL biosensor has a sensitive response to A β peptides with a linear range of 10 fM-0.1 μM and a detection limit of 3.25 fM. Considering prominent specificity, reproducibility, and stability, the proposed biosensor was used to assess recovery of A β peptides in human serum and the result was satisfactory. We believe that the established method would offer a useful analytical means for quantifying A β peptides in a biological matrix.
研究领域:
生物化学
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分析化学
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神经科学
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材料科学
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高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Xiufang Li,Jian Chen,Hui Liu,Zhiwei Deng,Jianbing Li,Tao Ren,Ling Huang,Wenqing Chen,Yanjing Yang,Shian Zhong
The study developed a simple, effective and inexpensive strategy for capturing, enriching and detecting circulating tumor cells (CTCs) by using folic acid (FA) as the targeting molecule instead of antibodies. This work constructed magnetic halloysite nanotubes (MHNTs) coated with biocompatible β-cyclodextrin (CD), and conjugated to FA via a PEG-Ad linker, to specifically capture the FA receptor (FR)-overexpressing cancer cells. The capture efficiencies of [email protected] β [email protected] for the Skov3, Hela and A549 cancer cells were 96.3%, 97.0% and 95.6% respectively. In addition, the nanoparticles were able to capture very low numbers of the cancer cells (25–500 cells/mL) from PBS and whole blood, as well as selectively capture the cancer cells over normal HEK 293 T cells. Furthermore, the captured cells were viable and grew normally in vitro , indicating the future potential of downstream analyses. This approach can be adapted for different CTCs, once the tumor-specific surface markers are identified and the efficacy of targeting ligands is established. Taken together, FA-conjugated MHNTs nanoparticles are a highly promising tool for isolating CTCs for the diagnosis and treatment of cancer.
研究领域:
纳米技术
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癌症生物学
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生物医学工程
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分子生物学
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药物递送系统
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Heijin Chen,Feng Yan,Xianfeng Jiao,Hao Wu,Xiaoyu Liang,Xuehua Shen,Hongzhao Fan,Zuotai Zhang
Solid amine adsorbent is recognized as an efficient CO 2 capture route for globe climate change mitigation. The pore structure of nano supports generally plays a crucial role in the CO 2 adsorption performance of solid amine adsorbents; however, the involving of costly and toxic pore-expanding agents limited their scalable application. Herein, an azeotropic-distillation nano-SiO 2 (AD-SiO 2 ) support was recycled from coal fly ash (CFA) using the CO 2 -assisted precipitation and n-butanol AD methods, and possessed a super pore volume of 2.52 cm 3 ·g −1 and an average pore size of 36.6 nm. After impregnating the triethyltetramine, tetraethylenepentamine, pentaethylenehexamine (PEHA), or polyethyleneimine, the synthesized amine@AD-SiO 2 adsorbents achieved the excellent adsorption capacity of 3.50–6.13 mmol·g −1 and the fast adsorption kinetics, owing to the superior pore structure of AD-SiO 2 support. Based on the viscosity, TGA and liquid-NMR characterization, we explained that the large amine molecule would block the CO 2 diffusion channel and thus restrict the active sites, while the small amine molecule would evaporate during the regeneration process. Inspiringly, the PEHA@AD-SiO 2 adsorbent possessed the excellent cyclic stability with merely 0.18 % decay per cycle and achieved a final CO 2 uptake of 4.24 mmol·g −1 over 50 cycles. This study notably improved the long-term adsorption capacity of solid amine adsorbents via the pore-expanded AD-SiO 2 support from CFA, thereby is a low-carbon technology for large-scale industrial CO 2 capture.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
BMC MICROBIOLOGY
·
2026
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IF 5.4
Shi Le-Nan,Han Yang-gang,Liang Hai,Li Tiejun,Shi Hui,Jin Lei
Background The rapid expansion of the aquaculture industry has intensified the discharge of nitrogen-rich wastewater, posing significant ecological risks. Biological treatment with HN-AD bacteria is sustainable, but most conventional strains lack long-term environmental resilience. The key bottleneck is identifying novel, robustly adaptable strains, especially spore-forming Gram-positive bacteria. Moreover, the non-canonical metabolic mechanisms that distinguish such strains remain largely unexplored. This study isolated a novel HN-AD strain, Paenibacillus glycanilyticus DQ-1, from aquaculture sludge and investigated its nitrogen removal performance and genomic metabolic pathways. Methods Strain DQ-1 was isolated and identified through 16 S rRNA gene sequencing and morphological analysis. Its nitrogen removal efficiency was evaluated under aerobic conditions with different nitrogen sources, including ammonium nitrogen (NH₄ + -N), nitrate nitrogen (NO₃ − -N), total inorganic nitrogen (TIN) and hydroxylamine nitrogen (NH 2 OH-N). Whole-genome sequencing was conducted using the PacBio Sequel II platform to identify functional genes and reconstruct the nitrogen metabolic pathways. Results Strain DQ-1 achieved 93.26% NH₄⁺-N and 100% NO₃⁻-N removal within 36 h, with 95.92% TIN removal. Genomic analysis revealed a complete assimilatory nitrate reduction pathway ( nasAB and nirBD ). Notably, while the canonical hydroxylamine oxidoreductase gene ( hao ) is absent, the strain showed 74.67% NH 2 OH-N removal and harbors a putative ammonia monooxygenase gene ( amo ). These results suggest that DQ-1 employs a novel non-canonical pathway, distinct from known HN-AD models, for efficient nitrogen transformation. Conclusions P. glycanilyticus DQ-1 differs from typical HN-AD bacteria in its spore-forming ability and a novel hao -independent pathway. This study provides the first comprehensive genomic insight into the nitrogen removal capacity of P. glycanilyticus , expanding the microbial resource pool for sustainable environmental remediation.
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DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
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2025
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IF 5.4
Meng Kong,Zixuan Xu,Haifei Lu,Yumeng Chen,Yini Zhang,Xia Jiang,Ping Wang
Ethnopharmacological relevance ShengHui decoction (SHD), a traditional Chinese herbal formula, has long been used clinically to mitigate Alzheimer’s disease (AD), demonstrating significant efficacy in alleviating cognitive impairment. However, its molecular mechanisms remain insufficiently understood. Aim of the study To clarify the effects of SHD on oxidative stress–related markers and inflammatory cytokines in an aluminum chloride (AlCl 3 )-induced zebrafish model of AD and as well as uncover its underlying mechanisms in alleviating oxidative stress and neuroinflammation.. Materials and methods Ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was employed to identify the bioactive constituents of SHD. Behavioral tests and histopathological analyses were then conducted to evaluate the effects of SHD (0.74, 1.48, and 2.96 mg/mL) on apoptosis and amyloid-beta (Aβ) deposition in an AlCl 3 -induced AD zebrafish model. Biochemical assays and enzyme-linked immunosorbent assay were used to quantify superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), IL-1 beta (IL-1β), and IL-18.. Western blot analysis was performed to determine the protein expression of nuclear factor erythroid 2–related factor 2 (Nrf2), heme oxygenase-1 (HO-1), NAD(P)H quinone dehydrogenase 1 (NQO1), nuclear factor-kappa B p65 (NF-κB p65), phosphorylated NF-κB p65 (p–NF-κB p65), and NOD-like receptor family pyrin domain containing 3 (NLRP3). Results UPLC–MS/MS profiling identified phenolic acids, saponins, lignans/coumarins, flavonoids, and aliphatic alcohols as the main constituents of SHD. SHD significantly ameliorated cognitive deficits and histopathological abnormalities in AlCl 3 -induced zebrafish. Moreover, SHD attenuated oxidative stress by upregulating Nrf2, HO-1, and NQO1 expression, while downregulating AlCl 3 -induced levels of NF-κB p65, p–NF-κB p65, NLRP3 and pro-inflammatory factors. Conclusions SHD effectively improved cognitive performance and reduced AD-like pathological lesions in AlCl 3 -induced zebrafish. Importantly, SHD exhibits both antioxidant and anti-inflammatory activities by activating the Nrf2/HO-1 axis and inhibiting NF-κB signaling, thereby alleviating oxidative stress and neuroinflammatory responses in AD. These findings provide experimental evidence supporting SHD as a potential therapeutic agent for AD, and substantiate the traditional Chinese medicine principle of tonifying the kidney and replenishing essence in the treatment of AD.
研究领域:
药理学
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神经科学
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民族药理学
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分子生物学
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中医中药
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2026
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IF 6.7
Wenlong Guo,Yingjie Cheng,Haofan Yin,Sheng Li,Yunzhu Wan,Chenzhong Li,Cheng Jiang,Jianhua Zhou,Xiaopeng Yuan,Jiasi Wang
Blood-based biomarkers present a noninvasive approach for detecting and assessing Alzheimer’s disease (AD) pathophysiology, always by using sophisticated instrumentation for accurate detection. Here, we introduce CRISPR-AD, a CRISPR/Cas-based digital assay designed for the combined detection of protein and microRNA in blood. This method achieves a limit of detection (LOD) as low as 60 fg/mL for phosphorylated tau217 (p-tau217) and 0.5 fM for microRNA-34a-5p (miRNA34a), enabling successful detection in both AD patients and healthy individuals. We find that the combined use of these biomarkers improves the ability to distinguish between AD patients and healthy participants, particularly in individuals with mild cognitive impairment (MCI). Additionally, we have developed a portable device that integrates a smartphone as an imaging system for point-of-care testing (POCT), offering the potential for early stage AD screening. This study represents the first effort to evaluate the combined detection of blood protein and microRNA biomarkers for AD, underscoring the potential of multiple biomarker combinations for more accurate AD diagnosis.
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DOI
PubMed
高影响因子
Aging-US
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2019
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IF 5.515
Chen Diling,Guo Yinrui,Qi Longkai,Tang Xiaocui,Liu Yadi,Yang Xin,Hu Guoyan,Shuai Ou,Yong Tianqiao,Wang Dongdong,Xie Yizhen,Burton B. Yang,Wu Qingping
Recent studies have demonstrated circular RNAs (circRNAs) to be widely expressed and to have important physiological functions. However, the expression, regulation, and function of circRNAs in neuroglial cells are unknown. Herein, we characterized the expression, regulation, and function of circRNAs in astrocytes. Astrocyte circRNAs were identified by computational analysis of newborn SD rat primary astrocytes cultured with 20 g/L D-galactose. In this manner, 7376 circRNAs were identified, among which most circRNAs (5754) were derived from annot_exons, whereas 27 were antisense, 853 were exon/intron, 329 were intergenic, 41 were intronic, and 372 were one exon. Among these, circNF1-419 was demonstrated to regulate autophagy, in over-expressing circNF1-419 transfected astrocytes, through the PI3K-I/Akt-AMPK-mTOR and PI3K-I/Akt-mTOR signaling pathways. An adenovirus associated virus packaging system (virus titer 1 ×10 12 ), over-expressing circNF1-419 and injected into mouse cerebral cortex, showed autophagy enhancing activity by binding the proteins Dynamin-1 and Adaptor protein 2 B1 (AP2B1). This binding regulated aging markers (p21, p35/25, and p16) and inflammatory factors (TNF-α and NF-κB), and reduced the expression of Alzheimer’s disease marker proteins (Tau, p-Tau, Aβ 1-42 , and APOE), which delayed senile dementia. Transcriptome analysis of the brain showed that circNF1-419 improved other signaling pathways, especially those related to the synapses of SAMP8 mice. These findings provide novel insights into circNF1-419 and its potential usefulness for the diagnosis and treatment of dementia by regulating Dynamin-1 and AP2B1 mediated autophagy.
研究领域:
神经科学
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分子生物学
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细胞生物学
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衰老研究
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神经退行性疾病
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Yanyan Yu,Chao Yu,Tianxiao Yin,Shanshan Ou,Xiaoyu Sun,Xiangru Wen,Lin Zhang,Daoquan Tang,Xiaoxing Yin
An efficient ratiometric electrochemical biosensor for Cu 2+ determination was constructed using dual hydroxyl-functionalized poly (ionic liquid) (DHF-PIL) as the catalyst support. The DHF-PIL exhibited typical macroporous structure, which provided a high surface area of 39.31 m 2 /g for the sufficient loading of biomolecules . The specific recognition of Cu 2+ was accomplished by employing neurokinin B (NKB) for the first time, which could bind to Cu 2+ to form a [Cu II (NKB) 2 ] complex with high specificity. Meanwhile, a common redox mediator , 2, 2′-Azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) was modified into DHF-PIL by electrostatic interactions to act as an inner reference molecule, which provided a built-in correction for environmental effects and improving the detection accuracy. With this strategy, the developed electrochemical biosensor was capable of determining Cu 2+ with a linear range between 0.9 and 36.1 μM and low detection limit (LOD) and quantification limit (LOQ) of 0.24 and 0.6 μM, respectively. The sensor also displayed a satisfactory selectivity against a series of interferences in the brain, including metal ions , amino acids and other endogenous compounds . Accordingly, the present biosensor was successfully applied to evaluate Cu 2+ levels in normal and AD rats.
研究领域:
分析化学
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生物医学工程
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神经科学
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Hanbang Liu,Liping Yang,Danhua Yuan,Guangye Liu,Jiacheng Xing,Yunpeng Xu,Zhongmin Liu
For air separation, the separation method relying on adsorbents has attracted wide attention as a low energy consumption process, and the development of O 2 -selective adsorbents is of great significance. Metal-organic frameworks with coordinatively unsaturated transition metal sites have great potential in O 2 -selective adsorption, but most of them cannot be completely regenerated at ambient temperature or can only be effective at very low temperatures. Herein, we report two mixed-ligand metal–organic framework adsorbents [M(AIP)(BPY) 0.5 ·H 2 O] n ·2nH 2 O with coordinatively unsaturated cobalt(II) and nickel(II) sites, which can preferentially adsorb O 2 versus N 2 , and their IAST O 2 /N 2 selectivities are both significantly greater than 1 at 25 °C. These materials exhibited excellent stability and had no loss of adsorption capacities even after immersion in water for 7 days. More importantly, O 2 adsorption–desorption cycle experiments showed that these adsorbents can be completely regenerated at ambient temperature after adsorbing O 2 , and the breakthrough experiments further confirmed their dynamic O 2 /N 2 separation potential and regeneration ability. The results of theoretical calculations suggested that the interactions between adsorbents and O 2 are stronger than those of N 2 , and they have relatively obvious differences in the N 2 and O 2 interaction energies. This work provides inspirations for searching for metal–organic frameworks that can selectively adsorb O 2 at ambient temperature.
研究领域:
材料科学
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化学工程
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高影响因子
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2022
·Vol. 106
·期 7
·2403-2414
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IF 5.56
Zhao Wenyu,Xie Haisheng,Zhang Xuehong,Wang Zhilong
Abstract Water–oil interface of bacterial cell-stabilized Pickering emulsions is an exceptional habitat for microbial assimilation of both hydrophobic nutrients solubilized in oil phase and hydrophilic ones solubilized in water phase. Crystal substrate inhibition, i.e., decreasing phytosterol degradation with the increase loading of crystal phytosterols, is always observed during microbial transformation of phytosterols into steroid synthons in Mycolicibacterium sp (China Center of Industrial Culture Collection, CICC 21,097) cell-stabilized Pickering emulsions. In the present work, we confirmed that crystal substrate inhibition was attributed to the interaction between M. neoaurum and phytosterol crystals that led to the detachment of bacterial cells from the oil–water interfaces in bacterial cell-stabilized Pickering emulsions. Under the selected operation condition (25 ml BEHP per 40 ml water, 60 g/L glucose, 25 g/L phytosterols), the product androst-4-ene-3, 17-dione (AD) and androsta-1, 4-dien-3, 17-dione (ADD) concentration increased linearly with the progress of microbial transformation and reached almost 6 g/L at the 11 th day. This is a paradigm for microbial transformation of crystal substrates as well as in the presence of other surface active additives (such as chitosan and nonionic surfactants) in bacterial cell-stabilized Pickering emulsions. Key points • Microbial transformation of crystal phytosterols in Pickering emulsions • Crystal substrate inhibition occurring during microbial transformation • Interaction between phytosterol crystals and bacterial cells leading to demulsification
研究领域:
生物技术
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微生物学
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化学工程
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药学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2017
·Vol. 5
·期 15
·2823-2831
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IF 4.543
Dan Zhao,Xiaoqing Yi,Jiaqi Xu,Gongdao Yuan,Renxi Zhuo,Feng Li
Using the host–guest interaction between β-cyclodextrin (β-CD) and adamantane (Ad), and borate formation between phenylboronic acid (PBA) and cis-diols, a smart pH-responsible targeting drug delivery nanovehicle, PBA–PEG–CD/Ad-lys(Diol)–PCL, was prepared via one-step self-assembly. Under physiological conditions, the targeted PBA function could be restrained by binding PBA with diol components located at the interface of self-assemblies, and hydrophilic PEG segments could be shielded simultaneously. When the environmental pH decreased, the PBA groups could be unbound and exposed on the surface of self-assemblies, leading to recovery of its targeted function, as shown using fluorescence spectroscopy, in vitro cell toxicity, and uptake. Under acidic conditions, PBA–PEG–CD/Ad-lys(Diol)–PCL/Dox showed significantly increased uptake and toxicity toward HepG2 cells in comparison with the control group. The smart vehicles were further utilized to test their efficiency in overcoming drug resistance in chemotherapy. Compared with free Dox, PBA–PEG–CD/Ad-lys(Diol)–PCL delivered six times more Dox into MCF-7/ADR cells and showed greater toxicity toward the ADR cells. As a result, this may be a facile strategy toward constructing efficient targeting vehicles through the rational utilization of noncovalent interactions.
研究领域:
药剂学
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纳米技术
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药物递送系统
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癌症研究
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
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2024
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IF 4.4
Zhang Jinpeng,Cui Xinyuan,Zhao Shuo,Chang Zenghui,Zhang Junshuo,Chen Yufeng,Liu Jiale,Sun Guohao,Wang Yiyuan,Liu Yuanyuan
Objective: Our previous studies substantiated that the biological activity of Schisandra chinensis lignans during the treatment of Alzheimer’s disease (AD) was mediated by neurotransmitter levels, and 15 of its active components were identified. However, the pharmacokinetic and pharmacodynamic relationship of Schisandra chinensis lignans has been less studied. The objective of this study was to investigate the relationship between the pharmacokinetics and pharmacodynamics of Schisandra chinensis lignans in the treatment of AD, and to establish a pharmacokinetic-pharmacodynamic (PK-PD) model.Methods and Results: Herein, we established a microdialysis-ultra performance liquid chromatography-triple quadruple mass spectrometry (MD-LC-TQ-MS) technique that could simultaneously and continuously collect and quantitatively analyze the active compounds and neurotransmitters related to the therapeutic effects of Schisandra chinensis in awake AD rats. Eight lignans were detected in the hippocampus, and a PK-PD model was established. The fitted curves highlighted a temporal lag between the maximum drug concentration and the peak drug effect. Following treatment, the levels of four neurotransmitters tended to converge with those observed in the sham operation group.Conclusion: By establishing a comprehensive concentration-time-effect relationship for Schisandra chinensis lignans in AD treatment, our study provides novel insights into the in vivo effects of these lignans in AD rats.
研究领域:
药理学
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神经科学
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分析化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）