BIOMEDICAL MICRODEVICES
·
2025
·Vol. 27
·期 3
·1-11
·
IF 3.3
Bashir Muhammad Noman,Wang Miaomiao,Chen Yating,Yuan Yuxuan,Noureen Beenish,Liu Minggao,Liu Yage,Qu Zhan,Du Liping,Wu Chunsheng
The urgent need for adenosine triphosphate (ATP) detection spans various fields, particularly in biology and medicine. Developing a simple, quick, label-free, and highly sensitive biosensor for ATP detection is crucial. In this study, we created a label-free biosensor using a field-effect device, specifically an electrolyte-insulator-semiconductor (EIS) sensor, which was functionalized with aptamer and graphene. We prepared a nanocomplex by combining graphene with bovine serum albumin (BSA) in PBS and subjecting it to ultrasonication. This Graphene/BSA mixture was then combined with 70% glutaraldehyde to form the Graphene/BSA/GA nanocomplex. The successful modification of the EIS biosensor surface with Graphene/BSA/GA and aptamer immobilization was confirmed using atomic force microscopy (AFM), which indicated successful molecule attachment through surface roughness. Electrochemical characterization revealed that the biosensor is sensitive to ATP concentrations ranging from 0.1 nM to 100 nM, with a detection limit as low as 0.32 nM. Statistical analysis demonstrated the biosensor’s high sensitivity and specificity for ATP. Furthermore, the biosensor maintained stable performance for ATP detection over a period of 5 days. This sensing approach effectively detected ATP with outstanding performance, showing significant potential for advancing label-free ATP detection technologies. Graphical abstract
研究领域:
生物传感器
·
生物电子学
·
分析化学
·
纳米技术
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMER
·
2018
·
IF 3.483
Yanming Lin,Ling Wang,Jinsheng Zhou,Lin Ye,Huiyuan Hu,Zhongkuan Luo,Li Zhou
In this study, we grafted brush-like CBMA copolymer brushes onto the surface of PVA hydrogel membrane through photo-induced electron transfer–reversible addition fragmentation chain transfer (PET-RAFT) polymerization. ATR-FTIR, XPS , AFM , UV–Vis absorption spectra and elemental analysis were adopted to characterize the hydrogels, and equilibrium swelling, water contact angle, vitro cytotoxicity assay, protein adsorption and cells adhesion experiments were used to evaluate the related property of hydrogels. The results of ATR-FTIR and XPS showed that pCBMA was successfully grafted onto the surface of PVA hydrogel membrane. The Ra roughness of hydrogel surface in AFM increased slightly from 5.44 nm to 6.12 nm. Transmittance of the PVA-g-pCBMA hydrogel in the visible range (380 nm-780 nm) was remaining at 91.2%–97.0%. The optimal reaction conditions (CPADB: 1.20 mmol/L, Eosin Y: 0.03 mmol/L and CBMA : 358 mmol/L) were confirmed through control experiments. The equilibrium swelling and water contact angle showed that the swelling ratio increased from 2.06 cm 3 g −1 to 3.65 cm 3 g −1 , while the water contact angle decreased from 45.94° to 18.06° with the increase of grafting ratio (23.92%–55.18%). In vitro cytotoxicity assay by CCK-8 showed that the cells relative viability remained at least 90.55%. Anti-protein adsorption experiment illustrated approximately 57.2% improvement of anti-protein capacity at 37 °C, while cells adhesion experiment also showed about 64.57% improvement of anti-cells capacity. These results revealed the applicability of the oxygen tolerant PET-RAFT mechanism on the PVA modification and it was effective, feasible and non-cytotoxic method to prepare the other functional PVA hydrogel membrane.
研究领域:
材料科学
·
聚合物化学
·
生物医学工程
·
表面科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2023
·Vol. 58
·期 12
·6386-6398
·
IF 3.3
Zi-wei Liu,Jun-ru Qi,Ya-ting Song,Cheng-wei Ruan,Jin-song Liao
Summary The properties of pectin extracted by enzyme-assisted nitric acid (EAE-N) and pectin extracted by enzyme-assisted oxalic acid (EAE-O) were analysed and compared with pectin extracted by nitric acid (AE-N) and pectin extracted by oxalic acid (AE-O) systematically. The yield of pectin obtained by the enzyme-assisted acid method (23.73% ~ 25.60%) was significantly higher than that by acid extraction method (19.75% ~ 20.60%). Monosaccharide analyses demonstrated that the enzyme-assisted acid method was able to attain more branched rhamnogalacturonan I (RG-I) regions (36.59% ~ 42.06%) than using acid solely (31.01% ~ 34.58%). Fourier transform infrared spectroscopy (FT–IR) and nuclear magnetic resonance ( 1 H NMR) analyses indicated that there was no obvious difference in the characteristic structure of pectin. The atomic force microscopy (AFM) images revealed that EAE-N and EAE-O had more short side chains and cross-link compared with AE-N and AE-O. Thermogravimetric analysis (TGA) shows that EAE-N and EAE-O have good thermal stability. All pectin samples showed a shear-thinning fluid flow behaviour and an elastic gel-like behaviour. The results suggested that enzyme-assisted acid provided a viable alternative method for the extraction of pectin.
研究领域:
食品科学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Nanomaterials
·
2024
·Vol. 14
·期 16
·1371
·
IF 4.4
Yi Xing,Weilei Wang,Weili Liu,Zhitang Song
The stability of slurries used for chemical mechanical polishing (CMP) is a crucial concern in industrial chip production, influencing both the quality and cost-effectiveness of polishing fluids. In silicon wafer polishing, the conventional use of commercial neutral silica sol combined with organic bases often leads to slurry instability. To address this issue, this study proposes organic amines—specifically ethanolamine (MEA), ethylenediamine (EDA), and tetramethylammonium hydroxide (TMAOH)—as catalysts for synthesizing alkaline silica sol tailored for silicon wafer polishing fluids. Sol–gel experiments and zeta potential measurements demonstrate the efficacy of this approach in enhancing the stability of silica sol. The quantitative analysis of surface hydroxyl groups reveals a direct correlation between enhanced stability and increased hydroxyl content. The application of the alkaline silica sol in silicon wafer polishing fluids improves polishing rates and enhances surface flatness according to atomic force microscopy (AFM). In addition, electrochemical experiments validate the capability of this polishing solution to mitigate corrosion on silicon wafer surfaces. These findings hold significant implications for the advancement of chemical mechanical polishing techniques in the field of integrated circuit fabrication.
研究领域:
材料科学
·
化学工程
·
半导体制造
·
表面化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2015
·Vol. 6
·期 21
·3926-3933
·
IF 5.52
Shanshan Liu,Qibin Chen,Yujie Sheng,Jincheng Shen,Changjun Peng,Honglai Liu
Developing supramolecular helical assemblies with a controlled helix sense and revealing the forming mechanism of hierarchical helices for mimicking biological self-organization and physiological processes remain major challenges. In this work, a bottlebrush-like supramolecular polymer is prepared through the formation of a hydrogen bond between the keto group of polyvinylpyrrolidone (PVP) and the phenolic hydroxyl group of p-dodecylphenol (PDP), characterized by 1H NMR and FT-IR spectroscopy. CD spectra show an unsplit Cotton effect, suggesting the emergence of a helical structure in this supramolecular polymer brush (PVP–PDP). In particular, straightforward experimental evidence for the formation of hierarchical helices is obtained via TEM and AFM methods. We propose a detailed structural transition of the hierarchical helix or superhelix of PVP–PDP from primary to secondary, tertiary and even quaternary structures, resulting from the incorporation of bulky pendant PDP into the PVP backbone and the binding site of the hydrogen bond at ortho-position.
研究领域:
超分子化学
·
高分子科学
·
材料科学
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纳米技术
·
生物物理学
✓ 已验证
产品关联
DOI
高影响因子
CARBON
·
2015
·
IF 6.196
Hao Wu,Wenyuan Yi,Zhe Chen,Haitao Wang,Qiangguo Du
Janus graphene oxide (GO) nanosheets functionalized by amino-containing chemicals were prepared via Pickering emulsion template. A wax-in-water Pickering emulsion was used to mask one side of GO nanosheets in order to achieve asymmetric chemical functionalization. Janus particles were obtained by removing the oil phase. The successful reaction of epoxy groups on the surface of GO with amino-containing chemicals was confirmed by Fourier transform infrared spectroscopy (FT-IR) and thermal gravimetric analysis (TGA). The asymmetric surface structure of Janus GO nanosheets was detected by atomic force microscope (AFM) and X-ray diffraction (XRD). The efficient stabilization of an oil-in-water Pickering emulsion by Janus GO was proved. Polymer microspheres fabricated by using Janus GO as Pickering stabilizer had a more hydrophilic surface compared with those stabilized by symmetrically modified GO.
研究领域:
材料科学
·
化学
·
纳米技术
·
胶体科学
✓ 已验证
产品关联
DOI
高影响因子
CONSTRUCTION AND BUILDING MATERIALS
·
2023
·
IF 7.4
Tao Liu,Naisheng Guo,Xin Jin,Yiqiu Tan,Zhanping You,Shichao Cui,Zhaoyang Chu,Chenze Fang
Phase change materials (PCMs) can regulate asphalt temperature through thermal storage properties to prevent thermal damage in asphalt pavements. However, the effects of PCMs on the thermoregulation and rheological properties of asphalt require further study. In this study, polyurethane solid–solid phase change materials (PUSSPCMs) were prepared to impart thermoregulation properties to asphalt, and the effects of PUSSPCMs on the rheological properties and microscopic characterization of asphalt were investigated. The PUSSPCMs were produced by different soft segment mass fractions (70 %, 80 %, and 90 %), and they were used to prepare modified asphalt with varying contents (3 %, 5 %, and 7 %). Thermoregulation testing system, dynamic shear rheology (DSR), bending beam rheology (BBR) tests, differential scanning calorimetry (DSC), infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and atomic force microscopy (AFM) were conducted to determine the thermoregulation, rheological properties, and modification mechanism of PUSSPCM-modified asphalt, respectively. The results show that the thermoregulation property of PUSSPCM-modified asphalt improves with the increase of soft segment mass fraction and content, 7 % P90 asphalt exhibits the best thermoregulation property with the excellent delayed time (1105 s) and temperature difference (7.1 ℃). The high-temperature rutting resistance of the modified asphalt is enhanced by decreasing the soft segment mass fraction and increasing the content of PUSSPCMs, with the 7 % P70 being the best asphalt type. The 7 % P90 asphalt exhibits the optimum low-temperature creep performance due to a higher soft segment mass fraction. The modified asphalt may be a physical modification since no new characteristic peaks appear. With the increase of soft segment mass fraction, the PUSSPCMs in asphalt transform from elastomer to a ribbon-like structure. Furthermore, the microscopic roughness ( S q ) and Young's modulus of the modified asphalt decline with the increase in soft segment mass fraction and content of PUSSPCMs, implying an increase in microscopic crack resistance and a decrease in elasticity.
研究领域:
土木工程
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Weizhen Zhang,Zhilong Yang,Yuan Li,Qi Zhang,Shijun Zhang,Liqun Zhang
Epoxidized silicone rubber with an epoxy content of 5% (ESR-5) was introduced to improve the compatibility of the polyamide 6 (PA6)/poly(dimethylsiloxane) (PDMS) composite. The effects of the ESR-5 on the molecular structure, interfacial morphology, and toughness and thermal properties of the PA6/PDMS composite were investigated using nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared (FT-IR) spectroscopy, differential scanning calorimetry (DSC), scanning electron microscopy (SEM), atomic force microscopy (AFM), and dynamic thermomechanical analysis (DMA). The FT-IR spectra and torque rheological testing confirmed the reaction between the epoxy group of ESR-5 and the carboxyl group at the end of PA6. The AFM and SEM images confirmed that the ESR-5 altered the interfacial thickness and remarkably decreased the average size of the dispersed rubber particles, which enhanced the interfacial compatibility. At a rubber content of 20% by weight in PA6/PDMS composites, the optimum impact strength at −60 °C, which is 469% higher than pure PA6, was obtained with an ESR-5 content of 50% in rubber phase.
研究领域:
材料科学
·
高分子科学
·
复合材料
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 15
·2463-2473
·
IF 5.047
Fenbo Ma,Yongmei Ge,Nian Liu,Xiangchao Pang,Xingyu Shen,Bin Tang
A composite hydrogel with tunable mechanical properties has been fabricated and characterized in this study. We investigated its swelling degree, morphology, structure and thermal stability. Moreover, the effect of strontium chloride concentration on both the dynamic rheology and nanomechanical properties of the composite hydrogels was confirmed in this work. To eliminate the viscoelastic influences of hydrogels during nanomechanical tests, we first analyzed the elastic modulus of strontium alginate (Alg-Sr) and strontium alginate/chondroitin sulfate (Alg/CS-Sr) hydrogels via atomic force microscopy (AFM) using the rate-jump method. Chondrocytes were cultured with the Alg-Sr and Alg/CS-Sr hydrogels respectively. Cell viability assay reveals that the Alg/CS-Sr hydrogel possesses good cytocompatibility. Flow cytometry, qPCR and western blotting analysis suggest that the Alg/CS-Sr hydrogel exerts a positive effect on the inhibition of apoptosis and may exert anti-inflammatory effects in articular cartilage related applications. Furthermore, the preliminary in vivo study shows that the Alg/CS-Sr composite hydrogel facilitates the repair of cartilage in rabbit cartilage defect. Taken together, it is indicated that the Alg/CS-Sr composite hydrogel might be a promising scaffold to promote the repair of cartilage defects.
研究领域:
生物材料
·
组织工程
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
KOREAN JOURNAL OF CHEMICAL ENGINEERING
·
2020
·Vol. 37
·期 3
·497-504
·
IF 2.69
Qiao Sen,Cao Hongjie,Yang Yue,Jin Ruofei,Zhou Jiti
Membrane fouling is the main bottleneck that hinders the applications of membrane bioreactors (MBRs). 4,5-Dichloro-2-n-octyl-4-isothiazolin-3-one (DCOIT), as an environmentally-acceptable antifouling biocide, was mixed with Polyvinylidene fluoride (PVDF) to fabricate hollow fiber membrane via non-solvent induced phase separation (NIPS), which was able to effectively improve the antifouling performance of the membranes in this work. Overall research of the prepared membrane revealed that membranes with 3 wt% DCOIT exhibited the optimum antifouling performance. With the addition of DCOIT, hydrophilicity and pure water flux of 3 wt% DCOIT membranes maintained remarkable improvement by 22.9% and 64.6% than that of membranes without DCOIT. Meanwhile, the surface morphologies of 3 wt% DCOIT membranes were smoother than the control group in terms of SEM and AFM images, which was beneficial to alleviate membrane fouling. In antifouling experiments, the flux variation rate of membranes with 3wt% DCOIT filtrated in bull serum albumin, sodium alginate and humic acid solution were 81.42%, 54.25%, 50.5%, while membrane without DCOIT were 64.6%, 24.72% and 29%, respectively. Similar results were obtained by filtrating anaerobic sludge for 24 h. The flux variations of 3 wt% DCOIT membranes were 59.4%, 47.8% and 46.0%, respectively in three stages. However, the flux variations of membranes without DCOIT were 44.8%, 36.7% and 19.8%, respectively, which showed better antifouling ability and higher flux recovery efficiency The novel membranes would provide some theoretical basis and technical support for the rational combinations in elevating the overall antifouling properties of membranes.
研究领域:
环境科学
·
材料科学
·
化学工程
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
POLYMER
·
2022
·
IF 4.432
Bo Zhang,Songyue Liu,Lijie Yin,Ming Tian,Nanying Ning,Liqun Zhang,Wencai Wang
The reaction mechanism and the interfacial modulus gradient of the interfacial transition layer between the environmentally friendly dipping system (PAL) and the rubber have not been fully studied. In order to better understand the chemical structures and nano-mechanical properties of the interface between the dip layer and the rubber, we used advanced atomic force microscope (AFM) and nanoscale Fourier transform infrared spectroscopy (nano-FTIR) to perform detailed analysis in this article. The characterization of interfacial nano-mechanical properties and chemical structures is very significant for the illustration of interfacial adhesion mechanism. AFM can effectively characterize the detailed interfacial modulus gradient and the thickness of interface, while nano-FTIR can comprehensively measure the interfacial chemical structures and the distribution of chemical bonds. The results suggest that the thickness of the interface between the PAL dip layer and the rubber matrix is 175 nm, and the crosslinking density of the interfacial layer is high, which explains that the failure location of fiber/rubber composites after pull-out tests occurs on the side of the rubber matrix. Similarly, the microstructure of resorcinol-formaldehyde-latex (RFL) system was also characterized, and the preparation of RFL usually requires carcinogenic and toxic substances such as formaldehyde and resorcinol .
研究领域:
材料科学
·
复合材料
·
纳米技术
·
高分子科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Danle Lin,Ziyu Zhou,Mengdan Zhang,Songyou Yao,Lingling Yuan,Meng Xu,Xiaoyue Zhang,Xiaoli Hu
Biophysical and biochemical cues of biomaterials can regulate cell behaviors. Dental pulp stem cells (DPSCs) in pulp tissues can differentiate to odontoblast-like cells and secrete reparative dentin to form a barrier to protect the underlying pulp tissues and enable complete pulp healing. Promotion of the odontogenic differentiation of DPSCs is essential for dentin regeneration. The effects of the surface potentials of biomaterials on the adhesion and odontogenic differentiation of DPSCs remain unclear. Here, poly(vinylidene fluoride-trifluoro ethylene) (P(VDF-TrFE)) films with different surface potentials were prepared by the spin-coating technique and the contact poling method. The cytoskeletal organization of DPSCs grown on P(VDF-TrFE) films was studied by immunofluorescence staining. Using atomic force microscopy (AFM), the lateral detachment forces of DPSCs from P(VDF-TrFE) films were quantified. The effects of electrical stimulation generated from P(VDF-TrFE) films on odontogenic differentiation of DPSCs were evaluated in vitro and in vivo. The unpolarized, positively polarized, and negatively polarized films had surface potentials of −52.9, +902.4, and −502.2 mV, respectively. DPSCs on both negatively and positively polarized P(VDF-TrFE) films had larger cell areas and length-to-width ratios than those on the unpolarized films (P < 0.05). During the detachment of DPSCs from P(VDF-TrFE) films, the average magnitudes of the maximum detachment forces were 29.4, 72.1, and 53.9 nN for unpolarized, positively polarized, and negatively polarized groups, respectively (P < 0.05). The polarized films enhanced the mineralization activities and increased the expression levels of the odontogenic-related proteins of DPSCs compared to the unpolarized films (P < 0.05). The extracellular signal-regulated kinase (ERK) signaling pathway was involved in the odontogenic differentiation of DPSCs as induced by surface charge. In vivo, the polarized P(VDF-TrFE) films enhanced adhesion of DPSCs and promoted the odontogenic differentiation of DPSCs by electrical stimulation, demonstrating a potential application of electroactive biomaterials for reparative dentin formation in direct pulp capping.
研究领域:
生物材料
·
干细胞生物学
·
组织工程
·
牙科材料
·
细胞粘附
·
分化
·
信号转导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）