Small
·
2023
·
IF 13.3
Jinfeng Wu,Cheng Xu,Zifan Ye,Haibo Chen,Yipeng Wang,Kai Yang,Bing Yuan
The cell membrane permeabilization ability of immune defense antimicrobial peptides (AMPs) is widely applied in biomedicine. Although the mechanisms of peptide–membrane interactions have been widely investigated, analyses at the molecular level are still lacking. Herein, the membrane-specific action of a native AMP, As-CATH4, is investigated using a single-lipid tracking method in combination with live cell and model membrane assays conducted at different scales. The peptide–membrane interaction process is characterized by analyzing single-lipid diffusion behaviors. As-CATH4 exhibits potent antimicrobial activity through bacterial membrane permeabilization, with moderate cytotoxicity against mammalian cells. In-plane diffusion analyses of individual lipids show that the lipid molecules exhibit non-Gaussian and heterogeneous diffusion behaviors in both pristine and peptide-treated membranes, which can be decomposed into two Gaussian subgroups corresponding to normal- and slow-diffusive lipids. Assessment of the temporal evolution of these two diffusion modes of lipids reveal that the peptide action states of As-CATH4 include surface binding, transmembrane defect formation, and dynamic equilibrium. The action mechanisms of As-CATH4 at varying concentrations and against different membranes are distinguished. This work resolves the simultaneous mixed diffusion mechanisms of single lipids in biomimetic cell membranes, especially during dynamic membrane permeabilization by AMPs.
研究领域:
生物物理学
·
分子生物学
·
细胞生物学
·
生物医学科学
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产品关联
DOI
PubMed
高影响因子
Antioxidants
·
2023
·Vol. 12
·期 3
·552
·
IF 7
Abid Naeem,Chengqun Yu,Zhenzhong Zang,Weifeng Zhu,Xuezhen Deng,Yongmei Guan
Oxidants play a significant role in causing oxidative stress in the body, which contributes to the development of diseases. Rutin—a powerful antioxidant—may be useful in the prevention and treatment of various diseases by scavenging oxidants and reducing oxidative stress. However, low solubility and oral bioavailability have restricted its use. Due to the hydrophobic nature of rutin, it cannot be easily loaded inside hydrogels. Therefore, first rutin inclusion complexes (RIC) with hydroxypropyl-β-cyclodextrin (HP-βCD) were prepared to improve its solubility, followed by incorporation into xanthan gum-based (hydroxypropyl methylcellulose-grafted-2-acrylamido -2-methyl-1-propane sulfonic acid) hydrogels for controlled drug release in order to improve the bioavailability. Rutin inclusion complexes and hydrogels were validated by FTIR, XRD, SEM, TGA, and DSC. The highest swelling ratio and drug release occurred at pH 1.2 (28% swelling ratio and 70% drug release) versus pH 7.4 (22% swelling ratio, 65% drug release) after 48 h. Hydrogels showed high porosity (94%) and biodegradation (9% in 1 week in phosphate buffer saline). Moreover, in vitro antioxidative and antibacterial studies (Staphylococcus aureus,Pseudomonas aeruginosa, andEscherichia coli) confirmed the antioxidative and antibacterial potential of the developed hydrogels.Keywords:hydrogels;flavonoids;anti-oxidants;inclusion complexes;oral formulations
研究领域:
药剂学
·
生物材料
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药物化学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Jiahui Li,Boxuan Peng,Siyuan Yu,Fangzheng Zhao,Qiqi Han,Shuaibing Huang,Fengmin Jin,Jinfeng Xing
With the rapid development of industry, water pollution has become one of the important problems that harm the ecological environment and human health. The extensive use and random discharge of dyes have caused serious damage to water resources. Hydrogel bead is a promising material for dye adsorption due to its advantages of rich functional groups and high specific surface area. Here, acrylic acid sodium salt (AAS) and 2-acrylamide-2-methylpropanesulfonic acid (AMPS) as monomers, N,N’-Methylene-bis-acrylamide (MBA) as crosslinking agent, sodium alginate (SA) as semi-interpenetrating network component, laponite as nano-clay additive, and single 2′,4′,5′,7′ −tetrabromofluorescein disodium salt (EY)/ Triethanolamine (TEOA)/ diphenyl iodonium hexafluorophosphate (DPI) as green light polymerization initiating system were combined to prepare composite hydrogel beads within 10 s under green LED irradiation. The composite hydrogels were characterized by infrared spectroscopy (FTIR), thermogravimetric analyzer (TG), scanning electron microscopy (SEM), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The addition of sodium alginate and laponite can improve the mechanical properties of hydrogel beads. In the adsorption study of malachite green, the influence of time, temperature, dye concentration, pH, ionic strength, and other factors on the adsorption effect were investigated. The adsorption process was consistent with the second-order kinetic process and Langmuir adsorption. At room temperature, the highest adsorption capacity of the hydrogel beads for malachite green reached 829.63 mg/g. The PAAS-SL hydrogel beads maintained a high adsorption effect in the pH range (3–10), and the capacity reached 575.0 mg /g at pH 3, which had a good ability to resist low pH. In the adsorption–desorption experiments, the removal rate of PAAS-SL hydrogel beads for MG is still more than 80 % after 5 cycles. Our research provides a promising strategy for the efficient and rapid preparation of hydrogel beads with excellent properties.
研究领域:
环境科学
·
材料科学
·
水处理
·
高分子化学
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产品关联
DOI
高影响因子
Geoenergy Science and Engineering
·
2023
·
IF 0
Hanyi Zhong,Shusen Li,Wenlei Liu,Zhengsong Qiu,Ying Guan,Weian Huang
Effective filtration loss control under high temperature and high salinity environments is a great challenge in oil and gas drilling engineering. In this study, a core-shell structural nano-composite (CSNC) using precipitated nano-CaCO 3 as the core material and cross-linked polymer between acrylamide (AM), 2-acrylamido-2-methylpropanesulfonic acid (AMPS), acrylic acid (AA) and methylene-bis-acrylamide (MBA) as the shell was prepared by reverse emulsion polymerization . The structure of CSNC was characterized with Fourier infrared spectrum (FT-IR), thermogravimetric analysis (TGA), scanning electron microscope (SEM), transmission electron microscope (TEM) and particle size distribution analysis. The filtration control properties of CSNC particles were investigated in both aqueous solution and bentonite suspension with various contents of NaCl and CaCl 2 respectively. The high temperature and high pressure (HTHP) filtration properties between CSNC and typical high temperature resistant filtration reducers were compared. The filtration controlling mechanism of CSNC particles were probed through zeta potential analysis, X-ray diffraction (XRD) analysis and filter cake morphologies observation. The results indicated that for the 4% w/v bentonite suspension treated with 3% w/v CSNC, the low temperature and low pressure (LTLP) filtration loss and filter cake thickness decreased by 84.2% and 76.1% respectively after thermal aging at 220 °C. When the bentonite suspension was contaminated with 30% w/v NaCl and 0.5% w/v CaCl 2 respectively, incorporation of 1% w/v CSNC reduced the filtration loss by 72.5% and 86.9% after thermal aging at 200 °C. The core-shell structure, cross-linked structure, and salt tolerant sulfonic acid groups on the outer surface of CSNC in combination contributed to extraordinary filtration loss control under stringent high temperature and high salinity conditions.
研究领域:
石油工程
·
材料科学
✓ 已验证
DOI
CHEMICAL ENGINEERING JOURNAL
·
2023
·
IF 15.1
Shenghui Liu,Haotian Jiang,Yangyang Li
This work developed a novel self-healing tight UF membrane based on damages to membrane during operation and cleaning via blending AMPS-PANI into the membrane matrix, which can not only endow the membrane with physical and chemical self-healing properties, but also enable the membrane to obtain effective separation performance of inorganic salt and dyes. Taking AMPS-PANI membrane (M4) as an example, the self-healing test showed that the dye/salt rejection rates of M4 membrane recovered to near pre-damage levels (more than 98%) for the physical damage. As for chemical damage, the high-resolution XPS spectra of S 2p of M4 membrane after damage displayed a larger S-C-C peak area than that before damage. The above results prove that the membrane has self-healing ability after physical and chemical damage. The observed physical self-healing property is ascribed to the swelling behavior of AMPS hydrogels, the fluidity of hydrogel chains and strong hydrogen bonding, and owing the chemical self-healing property to the Michael addition reaction between carbon–carbon double bonds and free radicals . The pure water flux , dye/salt rejection rate, structure, mechanical properties and porosity of the self-healing tight UF membrane were systematically investigated to evaluate its feasibility as an actual filtration membrane for textile wastewater. The results of the research indicate that membrane including both physical and chemical self-healing is a promising way to improve water filtration membrane with self-healing ability.
研究领域:
膜科学
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环境工程
·
材料科学
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产品关联
DOI
高影响因子
APPLIED CLAY SCIENCE
·
2022
·
IF 5.907
Qinglu Chang,Guoqing Liu,Zhanpeng Dong,Xia Miao,Miaomiao Hu,Jintang Guo
Fluid loss agent (FLA) was adopted to reduce the fluid loss of cement paste to ensure the safety of cementing construction. With the increase of exploration depth, FLA applied at high temperatures has been urgently required. To meet the cementing requirements at 240 °C, the FLA with organic/inorganic intercalated microstructure was prepared. Anionic polymer FLA (ADIS) was comprised of 2-acrylamido-2-methylpropane sulfonic Acid (AMPS), N , N -dimethylacrylamide (DMAA), Itaconic acid (IA) and Sodium p-styrene sulfonate (SSS). The organic-inorganic hybrid FLA (MgAl-ADIS-LDH) was prepared via intercalating ADIS into Mg/Al hydrotalcite (MgAl-NO 3 -LDH) interlayers . The intercalated microstructure was observed by SEM , TG results exhibited that the intercalation of polymer ADIS in MgAl-NO 3 -LDH enhanced the thermal stability of overall FLA. When applied in cement, the cement with MgAl-ADIS-LDH showed a small water loss of 36 mL at 240 °C. Moreover, the structure avoided the decrease in the compressive strength of hardened cement resulted from traditional polymer FLA. Compared with the cement contains 1.6% bwoc ADIS, the compressive strength of specimen with MgAl-ADIS-LDH increased by 20.5% after cured for 3 days.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
Biomaterials Science
·
2023
·Vol. 11
·期 9
·3214-3226
·
IF 6.6
Haijun Fu,Jiayu Yang,Zongshan Shen,Yong Zhang,Shuhong Kuang,Lifeng Li,Zhengmei Lin,Xuetao Shi
The severe pain caused by oral ulcers seriously affects food intake and speech, bringing great inconvenience in daily life. Drug-loaded patches are mostly used to treat oral mucosal diseases such as oral ulcers and oral lichen planus, but their effects are limited because of the influences of saliva and muscle movement. To enhance the adhesion of drug-loaded patches used in the oral cavity, we designed antimicrobial peptides (AMPs)-modified polycaprolactone (PCL)–collagen nanosheets (APCNs). The internal layer is a bioactive and antibacterial collagen layer modified with antimicrobial peptides. The backing layer is a hydrophobic PCL layer with good mechanical strength that can reduce external influences. We have characterized and tested the APCNs. First, the APCNs exhibited continuous and strong adhesion to irregular buccal mucosa surfaces under wet conditions and external force action. Antibacterial experiments showed that the APCNs had high antibacterial activity against both Gram-positive bacteria and Gram-negative bacteria. Moreover, the APCNs showed good biocompatibility and promoted the adhesion of fibroblasts in vitro. Furthermore, APCNs treatment accelerated ulcer healing in a Sprague Dawley rat oral ulcer model. Our study developed antibacterial, wet-adhesive, and healing-promoting PCL-collagen nanosheets and demonstrated that these nanosheets could be promising adhesive therapeutic agents for the treatment of oral mucosal ulcers.
研究领域:
药学
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材料科学
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生物医学工程
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牙科
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组织工程
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产品关联
DOI
PubMed
高影响因子
SOIL & TILLAGE RESEARCH
·
2022
·
IF 7.366
Hongran Xiong,Hui Peng,Xi’e Ye,Yanrong Kong,Na Wang,Fenghong Yang,Ben-Hur Meni,Ziqiang Lei
Hydroxyethyl starch (HES) and 2-Acrylamide-2-methyl-1-propanesulfonic acid (AMPS) as raw material, calcium alginate (CA33) was used as the main carrier for regulating and controlling soil water storage , and in situ polymerization with inorganic clay sodium bentonite to prepare a novel type of superabsorbent HES-PAM/bentonite and HES/CA33-PAM/ sodium bentonite composite materials using in-situ polymerization. The prepared hydrogel was characterized by scanning electron microscope (SEM), infrared spectrum (IR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The water absorption/release properties of the superabsorbent polymer were explored. The results showed that the new superabsorbent material reached salt equilibrium within 30 min. The water absorption rates in distilled water, tap water, and 0.9 wt% saline solution were 1484, 312, and 121 g/g. The successful inarching of calcium alginate improved the soil's water holding capacity and provided a slow water release, which increased the water absorption rate of the superabsorbent materials in distilled water and saline solution by 25.8% and 10%, respectively. The influence of soil types, particle diameter, and the content of superabsorbent materials on inhibiting soil water evaporation was studied. Therefore, having great application potential in the agricultural production of saline-alkali soils.
研究领域:
材料科学
·
环境科学
·
农业科学
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产品关联
DOI
高影响因子
ACS Applied Polymer Materials
·
2021
·
IF 4.089
Yifeng Cai,Haomin Wu,Wen Yan,Zhiao Yu,Wencan Ma,Caixia Liu,Qiuhong Zhang,Xudong Jia
A series of stretchable single-ion polymer electrolytes (SIPEs) based on Pluronic and 2-acrylamido-2-methylpropane sulfonic lithium (AMPS) are presented. Owing to the microphase separation of the soft segment and hard segment, this SIPE family exhibits high stretchability in which SIPE-2 can be stretched up to 840% with a fracture strength of 7.40 MPa. High Li+ content (2.06 wt %) is rationally incorporated via covalently grafting AMPS segments onto the polymer backbone, and therefore, these quasi-solid-state SIPEs delivers a considerable ionic conductivity of 1.10 × 10–4 S cm–1 at 65 °C, a wide electrochemical stability window (>5.0 V), and a high lithium transfer number (tLi+ > 0.93). Consequently, the stretchable and highly conductive single-ion polymer electrolytes show great potential for stretchable and flexible batteries.
研究领域:
材料科学
·
电化学
·
聚合物科学
·
能源存储
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 41
·期 3
·345-355
·
IF 3.815
Liu Hui,Liu Xiao,Cao Yuan-Qiao,Liu Ming,Han Miao-Miao,Ji Sheng-Xiang
Antimicrobial peptides (AMPs) have been considered as an alternative to small molecule antibiotics since they are difficult to develop antimicrobial resistance. Hyperbranched polylysine (HPL), an AMP mimics, has gained attention due to its broad-spectrum antibacterial activities, but it also suffers from high toxicity. Here we report a facile strategy to engineer the toxicity of HPL by copolymerizing lysine (K) with a hydrophobic amino acid, e.g. , alanine (A), tryptophan (W) or phenylalanine (F), to afford hyperbranched random copolymers. These copolymers have comparable antibacterial activities to HPL while their cytotoxicities and in vivo toxicities are lowered when the type and content of hydrophobic amino acid and the size of copolymers are optimized. The G. mellonella infection model demonstrates that the copolymers are effective against the S. aureus infection in vivo . The copolymers kill the bacteria through the disruption of cell membranes and the bacteria do not develop resistance to the copolymers.
研究领域:
材料科学
·
生物医学工程
·
微生物学
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Materials
·
2025
·Vol. 18
·期 17
·4163
·
IF 3.2
Fei Gao,Peng Xu,Yongqing Zhang,Hui Zhang
To address scaling issues in oilfield water injection, a degradable terpolymer scale inhibitor MA-AA-AMPS (terpolymer)was synthesized via aqueous solution polymerization using maleic anhydride, acrylic acid, and 2-acrylamido-2-methylpropanesulfonic acid as monomers. Characterization confirmed the presence of carboxyl, sulfonic, and amide groups in the copolymer with good thermal stability. Scale inhibition tests showed that at 2% dosage, its scale inhibition efficiency exceeded 80%, remaining above 80% in the pH range of 3–8 and over 50% at 150 °C, with excellent tolerance to high-calcium environments. Biodegradation tests revealed BOD5/COD > 0.3, with a biodegradation rate exceeding 50% in 15 days and reaching 83.4% in 30 days, indicating environmental friendliness. This scale inhibitor effectively solves scaling problems in oilfield water injection systems.
研究领域:
石油工程
·
高分子化学
·
环境科学
·
阻垢技术
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Microbiology
·
2025
·
IF 19.4
Wang Beilun,Lin Peijun,Zhong Yuwei,Tan Xiao,Shen Yangyang,Huang Yi,Jin Kai,Zhang Yan,Zhan Ying,Shen Dian,Wang Meng,Yu Zhou,Wu Yihan
Artificial intelligence (AI) is a promising approach to identify new antimicrobial compounds in diverse microbial species. Here we developed an AI-based, explainable deep learning model, EvoGradient, that predicts the potency of antimicrobial peptides (AMPs) and virtually modifies peptide sequences to produce more potent AMPs, akin to in silico directed evolution. We applied this model to peptides encoded in low-abundance human oral bacteria, resulting in the virtual evolution of 32 peptides into potent AMPs. Of these, the 6 most effective were synthesized and tested against multidrug-resistant pathogens and demonstrated activity against carbapenem-resistant species Escherichia coli , Klebsiella pneumoniae and Acinetobacter baumannii , and vancomycin-resistant Enterococcus faecium . The most potent AMP, pep-19-mod, was validated in vivo, achieving over 95% reduction in bacterial loads in mouse models of thigh infection through both systemic and local administration. Our approach advances the automatic identification and optimization of AMPs.
研究领域:
人工智能
·
微生物学
·
药学
·
计算生物学
·
药物发现
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）