NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 13
·5723-5731
·
IF 3.269
Yue Sa,Yaru Guo,Xiaowei Feng,Man Wang,Ping Li,Yixue Gao,Xu Yang,Tao Jiang
Crystallinity is related to the degree of order and the crystal size of a given crystalline substance. It can affect the quality of hydroxyapatite (HA) and its abnormality can lead to diseases of hard mineralized tissues. Crystallinity index (CI) is a quantitative indicator of crystallinity. Various techniques, such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy, and many methods based on these techniques have been used to define the CI of HA. The present study compares these methods and summarizes their characters for hydrothermally synthesized HA crystals with different aging time. Additionally, correlation coefficients between CIs calculated by different methods and crystal size (R12) and correlation coefficients among various CIs (R22) were obtained from linear-regression analysis. Scanning electron microscopy (SEM) was utilized as a supplementary technique to observe the morphological changes during the HA aging process. The results showed larger crystal size, increased crystallinity and more regular morphology of HA with increased aging time. All the R12 and R22 were above 0.9 and there were no significant differences between R12. However, different CI-calculating methods showed individual characters or limitations during their application. Such results suggested XRD, FTIR and Raman techniques used in this study were generally consistent and efficient but their special characters should be considered during their application. Researchers should choose certain appropriate technique and method to obtain CI on the basis of sample characteristics and experimental conditions.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
MOLECULAR MICROBIOLOGY
·
2016
·Vol. 103
·期 4
·713-728
·
IF 3.761
Tan-Jun Wang,Yi-Ming Shan,Han Li,Wei-Wang Dou,Xin-Hang Jiang,Xu-Ming Mao,Shui-Ping Liu,Wen-Jun Guan,Yong-Quan Li
Summary Antibiotic-producing microorganisms have evolved several self-resistance mechanisms to prevent auto-toxicity. Overexpression of specific transporters to improve the efflux of toxic antibiotics has been found one of the most important and intrinsic resistance strategies used by many Streptomyces strains. In this work, two ATP-binding cassette (ABC) transporter-encoding genes located in the natamycin biosynthetic gene cluster, scnA and scnB , were identified as the primary exporter genes for natamycin efflux in Streptomyces chattanoogensis L10. Two other transporters located outside the cluster, a major facilitator superfamily transporter Mfs1 and an ABC transporter NepI/II were found to play a complementary role in natamycin efflux. ScnA/ScnB and Mfs1 also participate in exporting the immediate precursor of natamycin, 4,5-de-epoxynatamycin, which is more toxic to S. chattanoogensis L10 than natamycin. As the major complementary exporter for natamycin efflux, Mfs1 is up-regulated in response to intracellular accumulation of natamycin and 4,5-de-epoxynatamycin, suggesting a key role in the stress response for self-resistance. This article discusses a novel antibiotic-related efflux and response system in Streptomyces , as well as a self-resistance mechanism in antibiotic-producing strains.
研究领域:
微生物学
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药理学
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遗传学
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DOI
PubMed
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Chaonan Li,Ya Wang,Zihao Wang,Xiaogang Li
Although paraquat is a widely used herbicide, it is toxic to humans if ingested or absorbed through an open wound. To improve the safety of paraquat, a new formulation of paraquat based on photoresponsive polymers was exploited. Photoresponsive β-cyclodextrin polymer microspheres (AZO-CD) were synthesized via a host–guest interaction between β-cyclodextrin and azobenzene. AZO-CD were characterized by Fourier transform infrared spectrometry, circular dichroism, ultraviolet (UV) spectrophotometry, and thermogravimetric analysis, and their photoresponsiveness was also evaluated. AZO-CD were used to load paraquat, which yielded photoresponsive paraquat-loaded microspheres. For the paraquat-loaded microspheres, irradiation with UV light or sunlight induced the isomerization of azobenzene into the cis form. Then, the cis-azobenzene was liberated from the cavities of the β-cyclodextrin. The paraquat-loaded microspheres released paraquat continuously over time. Furthermore, under UV light, the herbicidal capacity of the paraquat-loaded microspheres against barnyard grass was comparable to that of free paraquat at the same dose. Our findings show that loading paraquat into AZO-CD provides a safe and environmentally friendly herbicide formulation.
研究领域:
农业化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
EUROPEAN JOURNAL OF MEDICAL RESEARCH
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2025
·
IF 3.4
Pingge Tian,Lei Xu,Fan Zou,Qian Jia,Yangjian Liu,Jin Chen,Pengzhen Wang
Background: Hypertrophic cardiomyopathy (HCM) is a common inherited heart disease characterized by left ventricular hypertrophy and excessive myocardial contraction. Endothelial cells (ECs) play a key role in the pathogenesis of HCM, and their secreted growth factors regulate the growth and function of cardiomyocytes. However, the specific molecular mechanisms and biomarkers associated with endothelial dysfunction in HCM remain unclear. Methods: Single-cell RNA sequencing (scRNA-seq) data were downloaded from the Gene Expression Omnibus (GEO) database (GSE255296), and bulk RNA sequencing data were obtained from GSE249925 and GSE180313. The scRNA-seq data were processed using the Seurat package for cell type annotation and batch effect removal. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was performed, and pathway activity was evaluated using the AUCell algorithm. SCENIC was applied to construct transcription factor-specific regulatory networks. Differential gene expression analysis was conducted using the limma package, and key genes were determined by intersecting differentially expressed genes (DEGs) with EC-specific highly expressed genes. The diagnostic efficacy of these key genes was assessed via receiver operating characteristic (ROC) curve analysis. The mRNA expression of genes in HUVECs treated with or without angiotensin II (Ang II) was assessed using qPCR. Cell viability was determined using the CCK-8 assay, and cell invasive ability was evaluated through Transwell assays to investigate the regulatory effect of TMTC1 overexpression on Ang II-induced injury in HUVECs. Results: Seven major cell types were identified, with cardiomyocytes and ECs being the main cell populations in HCM. KEGG enrichment analysis revealed significant activation of pathways such as the transforming growth factor-beta (TGF-β) signaling pathway in ECs. High-activity transcription factors NFIB, MECOM, and FLI1 were identified in ECs, whose target genes were enriched in processes related to cell morphogenesis. Three key genes-TMTC1, RPS6KA2, and F8-were selected based on differential expression and EC specificity. These genes were lowly expressed in HCM and exhibited high diagnostic efficacy, with area under the curve (AUC) values > 0.7. Ultimately, the CCK-8 assay results indicated that Ang II inhibited the viability of HUVECs, and this inhibitory effect could be reversed by the overexpression of TMTC1 (oe-TMTC1). Furthermore, Transwell assays indicated that Ang II impaired cellular migration, and this impairment could be restored by oe-TMTC1. Conclusion: The identified key genes and pathways provide new targets for therapeutic intervention and contribute to a deeper understanding of the pathogenesis of HCM.
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PubMed
高影响因子
Heliyon
·
2023
·
IF 4
Peng Zhang,Fang Cao,Jiqin Zhang,Ying Tan,Shengtao Yao
The past decades have witnessed great progress in nanoparticle‐based cancer‐targeting drug delivery systems, but their therapeutic potentials is yet to be fully exploited. In this research, temozolomide (TMZ) and chloroquine (CQ) were loaded into the mesoporous silica nanoparticles (MSNs), the surface was coated with polydopamine (PDA), and the complex was coupled with arginine-glycine-aspartic (RGD) to successfully prepare TMZ/CQ@MSN-RGD. RGD-MSNs accumulated more in the cell and tumor models than in unmodified MSNs in the in vitro and in vivo experiments and can directly induce apoptosis and autophagy in tumor cells. In addition, TMZ/CQ@MSN-RGD therapy enhanced the apoptosis effect of the RGD-MSNs in glioma. Therefore, the combination of autophagy inhibitor with chemotherapy drugs in nanocarriers may promote therapeutic efficacy in treating glioma.
研究领域:
肿瘤学
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纳米医学
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药理学
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DOI
高影响因子
Life-Basel
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2022
·Vol. 12
·期 9
·1436
·
IF 3.251
Ce Chen,Mingyue He,Xueting Li,Lidong Yu,Yi Liu,Yan Yang,Li Li,Jianbo Jia,Bingsheng Li
The Nrf2/ARE signaling pathway is a cell survival response pathway in response to environmental stresses. The Nrf2/ARE signaling pathway can be activated by stimulating cysteine residues at different positions in the Keap1. However, the epigenetic mechanisms of the Nrf2/ARE pathway under different stimuli are still poorly understood. In this study, we found that both hydrogen peroxide (H2O2) and Diethyl Maleate (DEM) activated the Nrf2/ARE signaling pathway at 120 hpf in zebrafish. H2O2regulated the demethylation of themaftpromoter by inhibiting the expression of methyltransferase. This promotes the mRNA expression of the Nrf2 binding factormaft, thereby promoting the downstream antioxidant genes. The methylation of the Nrf2/ARE signaling pathway was not significantly regulated by DEM. However, under oxidative stress, the methyltransferase inhibitors (decitabine and azacitidine) demethylated the promoter region ofmaft. It activated the expression of themaft, further improving the Nrf2/ARE signal pathway. At last, antioxidant target genes were activated. It was shown that H2O2and DEM cooperated with methyltransferase inhibitors, providing an important reference for the treatment of oxidative stress-related diseases and breaking new ground for the study of the mechanism of methyltransferase inhibitors in the process of tumor chemotherapy.Keywords:zebrafish;Nrf2/ARE signaling pathway;oxidative stress;methylation regulation
研究领域:
分子生物学
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毒理学
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表观遗传学
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发育生物学
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DOI
PubMed
高影响因子
CURRENT BIOLOGY
·
2024
·
IF 8.1
Xueyi Xue,Lu Wang,Aobo Huang,Zehao Liu,Xiaoyu Guo,Yuying Sang,Jian-Kang Zhu,Huiling Xue,Juan Dong
In Arabidopsis , stomatal development and patterning require tightly regulated cell division and cell-fate differentiation that are controlled by key transcription factors and signaling molecules. To identify new regulators of stomatal development, we assay the transcriptomes of plants bearing enriched stomatal lineage cells that undergo active division. A member of the novel regulators at the plasma membrane (NRPM) family annotated as hydroxyproline-rich glycoproteins was identified to highly express in stomatal lineage cells. Overexpressing each of the four NRPM genes suppressed stomata formation, while the loss-of-function nrpm triple mutants generated severely overproduced stomata and abnormal patterning, mirroring those of the erecta receptor family and MAPKKK yoda null mutants. Manipulation of the subcellular localization of NRPM1 surprisingly revealed its regulatory roles as a peripheral membrane protein instead of a predicted cell wall protein. Further functional characterization suggests that NRPMs function downstream of the EPF1/2 peptide ligands and upstream of the YODA MAPK pathway. Genetic and cell biological analyses reveal that NRPM may promote the localization and function of the ERECTA receptor proteins at the cell surface. Therefore, we identify NRPM as a new class of signaling molecules at the plasma membrane to regulate many aspects of plant growth and development.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2015
·
IF 4.143
Yan He,Fanfeng Zeng,Zhenghua Lian,Jianming Xu,Philip C. Brookes
Natural soil montmorillonite and kaolinite nanoparticles (NPs) were tested as efficient sorbents for organic contaminant (OC) removal through mimicking their natural environmental dispersive states. Sorption of both mineral NPs decreased with increasing pH with ionizable pentachlorophenol (PCP), but increased with pH with non-ionizable phenanthrene (PHE), within the pH range of 4–10. In contrast, sorption decreased consistently for both PCP and PHE, as a function of increasing ion concentration (0.001–0.1 mol L −1 ). Sorption differences were likely caused by the electrolytic conditions dependent upon surface chemistry of OCs and mineral NPs. The results confirmed that the highly dispersive soil mineral NPs would prevail over both engineered NPs and their regular μm-sized colloids for OC removal, due to their ecological advantages and higher sorption properties. This finding provided a realistic assessment of the environmental function of soil natural minerals in water once they are released from soil into OC polluted aqueous systems.
研究领域:
环境科学
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土壤学
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环境化学
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纳米地球化学
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污染控制
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DOI
高影响因子
LANGMUIR
·
2023
·
IF 3.9
Yitan Li,Yuguang Chen,Yan Li,Howard A. Stone,Amir A. Pahlavan,Steve Granick
The shapes of highly volatile oil-on-water droplets become strongly asymmetric when they are out of equilibrium. The unsaturated organic vapor atmosphere causes evaporation and leads to a strong Marangoni flow in the bath, unlike that previously seen in the literature. Inspecting these shapes experimentally on millisecond and submillimeter time and length scales and theoretically by scaling arguments, we confirm that Marangoni-driven convection in the subphase mechanically stresses the droplet edges to an extent that increases for organic droplets of smaller contact angle and accordingly smaller thickness. The viscous stress generated by the subphase overcomes the thermodynamic Laplace pressure. The oil droplets develop copious regularly spaced fingers, and these fingers develop spike-shaped and branched treelike structures. Unlike this behavior for single-component (surfactant-free) oil droplets, droplets composed of two miscible (surfactant-free) organic liquids develop a rim of the less volatile component along the droplet perimeter, from which jets of monodisperse smaller droplets eject periodically due to the Rayleigh–Plateau instability. When evaporation shrinks droplets to μm size, their shapes fluctuate chaotically, and ellipsoidal shapes rupture into smaller daughter droplets when subphase convection flow pulls them in opposite directions. The shape of the evaporating oil droplets is kneaded and sculpted by vigorous flow in the water subphase.
研究领域:
物理学
·
流体力学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 4
·1-6
·
IF 5.705
Wang Yi,Zhang Pu,Lu Qing,Wang Yue,Fu Wensheng,Tan Qin,Luo Weiping
A method is described for the fluorometric determination of hypochlorite. It is making use of molybdenum disulfide quantum dots (MoS 2 QDs) as a fluorescent probe. The QDs are prepared by hydrothermal reaction of sodium molybdate with glutathione. They possess diameters typically ranging from 1.4 to 3.8 nm, excellent stability in water, and blue photoluminescence (with excitation/emission peaks located at 315/412 nm and a quantum yield of 3.7%). The fluorescence of the QDs is statically quenched by hypochlorite, and the Stern-Volmer plot is linear. Hypochlorite can be detected in the 5–500 μM concentration range with a 0.5 μM detection limit. The method has been successfully applied to the determination of hypochlorite in spiked samples of tap water, lake water, and commercial disinfectants.
Schematic of a method for the fluorometric determination of hypochlorite using MoS 2 quantum dots as a fluorescent probe. It has been applied to hypochlorite assay in spiked samples of tap water, lake water, and commercial disinfectants.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2017
·Vol. 8
·期 3
·1020-1027
·
IF 3.247
Xiaotong Sheng,Jingmin Yan,Yue Meng,Yuying Kang,Zhen Han,Guihua Tai,Yifa Zhou,Hairong Cheng
This study was aimed at investigating the immunomodulating activity of Hericium erinaceus polysaccharide (HEP) in mice, by assessing splenic lymphocyte proliferation (cell-mediated immunity), serum hemolysin levels (humoral immunity), phagocytic capacity of peritoneal cavity phagocytes (macrophage phagocytosis), and NK cell activity. ELISA of immunoglobulin A (SIgA) in the lamina propria, and western blotting of small intestinal proteins were also performed to gain insight into the mechanism by which HEP affects the intestinal immune system. Here, we report that HEP improves immune function by functionally enhancing cell-mediated and humoral immunity, macrophage phagocytosis, and NK cell activity. In addition, HEP was found to upregulate the secretion of SIgA and activate the MAPK and AKT cellular signaling pathways in the intestine. In conclusion, all these results allow us to postulate that the immunomodulatory effects of HEP are most likely attributed to the effective regulation of intestinal mucosal immune activity.
研究领域:
免疫学
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分子生物学
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胃肠病学
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DOI
PubMed
高影响因子
GENE EXPRESSION PATTERNS
·
2022
·
IF 1.488
Jian Zhou,Yan-Jing Yang,Rui-Hai Gan,Yang Wang,Zhi Li,Xiao-Juan Zhang,Jian-Fang Gui,Li Zhou
Foxl2 plays conserved central function in ovarian differentiation and maintenance in several fish species. However, its expression pattern and function in fish embryogenesis are still largely unknown. In this study, we first presented a sequential expression pattern of zebrafish foxl2a and foxl2b during embryo development. They were predominantly expressed in the cranial paraxial mesoderm (CPM) and cranial venous vasculature (CVV) during somitogenesis and subsequently expressed in the pharyngeal arches after 48 h post-fertilization (hpf). Then, we compared the brain structures among zebrafish wildtype (WT) and three homozygous foxl2 mutants ( foxl2a −/− , foxl2b −/− and foxl2a −/− ; foxl2b −/− ) and found the reduction of the fourth ventricle in the three foxl2 mutants, especially in foxl2a −/− ; foxl2b −/− mutant. Finally, we detected several key transcription factors involved in the gene regulatory network of midbrain-hindbrain boundary (MHB) patterning, such as wnt1 , en1b and pax2a . Their expression levels were obviously downregulated in MHB of foxl2a −/− and foxl2a −/− ; foxl2b −/− mutants. Thus, we suggest that Foxl2a and Foxl2b are involved in MHB and the fourth ventricle development in zebrafish. The current study provides insights into the molecular mechanism underlying development of brain ventricular system.
研究领域:
发育生物学
·
分子生物学
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DOI
PubMed
高影响因子
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