Materials Today Communications
·
2020
·
IF 2.678
Wenpo Feng,Chenxi Feng,Binbin Wang,Aihua Jing,Guangda Li,Xichao Xia,Gaofeng Liang
An amorphous calcium phosphate (ACP) for drug delivery was synthesized by using microwave-assisted hydrothermal synthesis with ATP-Na 2 provided a source of phosphorus. This ACP was characterized by X-ray power diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy(SEM), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) specific surface area and hydrodynamic sizes. In vitro cytotoxicity evaluation was used by Mg-63 cells. Doxorubicins (Dox) were used as a model drug to evaluate the drug loading and release abilities. Results show the ACP, synthesized at 120 °Cand pH9 for 30 min, was consisted of aggregated porous microspheres, has essentially no cytotoxicity, moderate drug release abilities, and has strong drug-holding capacity. As an anticancer drug delivery, it has a high ability to damage and kill cancer cells. So, it is promising for applications in drug delivery.
研究领域:
生物材料学
·
药剂学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xinran Shi,Liang Ji,Yuanyuan Hu,Jinyu Gu,Liming Wang,Wenwen Lu,Jiali Meng,Yan Du,Lingzhong Huang,Dongxia Nie,Yanyan Yu
Since adenosine triphosphate (ATP) and reactive oxygen species (ROS) are closely related to neuron functions and the pathogenesis of Alzheimer's disease , herein, the first example of using a dual-responsive zeolitic imidazolate framework-90 (ZIF-90) to fabricate a single fluorescent probe , CuNCs/ [email protected] /ZIF, was reported, which specifically and sensitively responded to intracellular level fluctuations of ATP and ROS both in neuronal and tumor cells without obvious cross-talking. Through a stepwise in situ growth method, ROS-responsive glutathione-stabilized copper nanoclusters (GSH-CuNCs) and double tagged ATP aptamer hybrid chain (P1-P2) were prepared and respectively encapsulated into the inner and outer ZIF-90. The obtained CuNCs/ [email protected] /ZIF probe as a tandem system could firstly respond to ATP in an “off-to-on” mode, in which, the quenched fluorescence of FAM by BHQ1 attached on P2 was recovered through the competitive displacement between ATP and ZIF-90, leading to the release of FAM from ZIF-90. Upon collapse of the outer ZIF-90, the strong emission of CuNCs enclosed in the inner ZIF-90 was subsequently quenched in the presence of ROS. Accompanied with high sensitivity, selectivity and accuracy, the resultant fluorescent probe exhibited good performance and properties for tracking the level variations of ATP and ROS in SH-SY5Y and Hela cells under different stimulations. This strategy highlighted the potential of the ZIF-90 based nanocomposite for multi-targets detection, and also provided a value tool for the early diagnosis of diseases.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
AQUACULTURE
·
2021
·
IF 4.242
Xiaoping Tan,Yang Hu,Shenye Qu,Gaoxue Wang,Tianqiang Liu
Noxious pathogens always lead to high rates of mortality, which results in severe economic losses in aquaculture. Currently, gyrodactylosis treatments mainly rely on unapproved chemicals, which are not always satisfactory. According to the previous study, arctigenin is an efficient natural product for controlling gyrodactylosis. For the sake of seeking high-efficiency compounds, a new arctigenin derivative (CCA) was synthesized. Next, we evaluated the anthelmintic activity of CCA in vitro and in vivo , and studied the mechanism of action. The number of Gyrodactylus kobayashii significantly reduced as compared with the control group, the EC 50 value at 24 and 48-h after CCA treatment was 0.616 and 0.451 mg/L, respectively, with EC 100 at CCA concentration of 3 mg/L after 1 h bath treatment. In vitro results showed that CCA can rapidly reduce the amount of G. kobayashii and inhibit its mobility that could normally lead to death. The concentration and duration of CCA were positively correlated with the mortality of G. kobayashii . In addition, CCA displayed 100% efficacy against G. kobayashii at 4.0 mg/L after only 20 min exposure. Acute toxicity assay demonstrated that the LC 50 of CCA against goldfish was 5.012 mg/L, which was 8.13 times higher than that of the EC 50 . Furthermore, the results of confocal microscope study revealed that CCA leaded to the epidermal structure and dissolved muscles of worms. Moreover, the ATP level of worms were decreased sharply after both in vitro and in vivo exposures with CCA. The anthelminthic mechanism of CCA against gyrodactylids was related to epidermal structure damages and energy insufficient. From the previous study, the EC 50 of CCA against G. kobayashii (0.616 mg/L) was much lower than the EC 50 (1.85 mg/L) of arctigenin ( Tu et al., 2018 ). In summary, CCA is more effective than arctigenin and has great potential in control of Gyrodactylus infections.
研究领域:
水产养殖
·
寄生虫学
·
药理学
·
兽医学
✓ 已验证
DOI
高影响因子
LUMINESCENCE
·
2021
·Vol. 36
·期 7
·1584-1591
·
IF 2.464
Yaning Qin,Zhaoyue Yan,Rui Liu,Chaojie Qu,Haichen Mao,Lingbo Qu,Ran Yang
In this work, novel types of nitrogen-doped carbon dots (N-CDs) were prepared from citric acid and glycine (GLY) as precursors through a simple pyrolysis method. The GLY-CDs showed strong fluorescence with a fluorescence quantum yield as high as 33.34% and good water solubility. The fluorescence of GLY-CDs could be selectively quenched by iron(III) ion (Fe 3+ ) resulting in the non-fluorescent complex. Due to the high affinity of Fe 3+ to adenosine-5′-triphosphate (ATP), the fluorescence of the GLY-CDs in GLY-CDs-Fe 3+ could be recovered by ATP. Thereby, quantitatively fluorescent turn-on detection of ATP could be achieved. The fluorescence recovery ratio was linearly proportional to the concentration of ATP with a detection limit as low as 15.0 nM, indicating the CDs have high sensitivity. The GLY-CDs were successfully employed in the detection of ATP in serum and cell lysates.
研究领域:
分析化学
·
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 12
·9702-9708
·
IF 3.201
Hengchang Ma,Yucheng Ma,Lei Lei,Manyi Yang,Yanfang Qin,Ziqiang Lei,Lu Chang,Tao Wang,Yuan Yang,Xiaoqiang Yao
A set of multiple cation-doped linear polymers (abbreviated as OPY-1,2-BE, OPY-1,4-BB, OPY-1,8-BO, OPY-1,4-OBB) synthesized from a dipyridine derivative (OPY) and dibrominated compounds were employed as fluorescent probes for adenosine triphosphate (ATP) sensing. Among them, OPY-1,8-BO is particularly able to recognize ATP over its related compounds adenosine diphosphate (ADP), adenosine monophosphate (AMP), and other phosphate anions, with very desirable switch off–on performances. The interesting phenomenon of aggregate-induced emission (AIE) is proposed as the main reason for the ATP sensing, which is due to the ATP being more negative than the polymers and therefore there is more opportunity for connections with the multiple cation doped polymers through electrostatic attractions. Cell imaging measurements were carried out and demonstrated that even though these probes bear the same OPY core and only slightly different linkers, they are able to image different cell environments. The controlled cell imaging verified that the OPY-1,8-BO probe has ATP-specific recognition in living cells.
研究领域:
材料科学
·
分析化学
·
生物化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 43
·8525-8531
·
IF 4.543
Hengchang Ma,Manyi Yang,Caili Zhang,Yucheng Ma,Yanfang Qin,Ziqiang Lei,Lu Chang,Lei Lei,Tao Wang,Yuan Yang
Aggregation-induced emission (AIE)-active compounds are attractive fluorescent materials for applications in chemical and biological sensing. The AIE effect of such materials amplifies changes in the fluorescence signal due to the physical state transformation from aggregation to disaggregation, which can be employed for detecting various analytes with high sensitivity. In particular, specific bio-active analyte recognition is not only very interesting but also challenging. In this paper, we report a set of novel AIE-active fluorescent probes containing pyridiniums and boric acid groups (TPA-PP, TPA-PPA-1, TPA-PPA-2, TPA-PPA-3), which has been developed for adenosine 5′-triphosphate (ATP) recognition. These probes with two types of interaction modes and multiple connection sites toward ATP molecules are able to selectively discriminate ATP among other bioactive anions with a significant enhancement in fluorescence emission. In particular, in the application of cell imaging, as the number of positive charges and boric acid group increased further, the probes could penetrate into cells, and then enter into the nucleus very specifically. These results clearly demonstrate that the newly developed sensors are suitable for specific tracing of different cell organelles with a height visualization and retention ability. Therefore, all of them are confirmed as promising alternatives for live cell imaging in the future.
研究领域:
生物化学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2013
·
IF 1.977
Lina Zhong,Feifei Xing,Yueling Bai,Yongmei Zhao,Shourong Zhu
Aspartic functionalized water-soluble perylene diimide, N , N ′-di(2-succinic acid)-perylene-3,4,9,10-tetracarboxylic diimide (PASP) has two absorbance maximums at 527 and 498 nm ( ε ≈ 1.7 × 10 4 L cm −1 mol −1 ) and two emission peaks at 547 and 587 nm respectively. Emission intensities decrease with the increase of PASP concentrations in 20–100 μM ranges. Spectral titrations demonstrate that each PASP can coordinate to two Cu 2+ ions in the absence of HEPES buffer. Its stability constant is estimated to be about 1.0 × 10 12 L 2 mol −2 at pH 7.20 and its coordinate stoichiometry increased to 7.5 in the same pH in the presence of HEPES buffer. The emission of PASP will be completely quenched upon formation of Cu 2+ complex. The lowest “turn-off” fluorescence detection limit was calculated to be 0.3 μM Cu 2+ . PASP–Cu solution was used as a “turn-on” fluorescence biosensor to detect ATP. The sensitivity towards ATP is 0.3 μM in 50 mM HEPES buffer at pH 7.20, which is one of the most sensitive fluorescence sensors.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2022
·Vol. 41
·期 1
·57-63
·
IF 5.56
Ziwei Su,Xiangxiang Zhang,Weichen Wang,Mingdong Dong,Xiaojun Han
Comprehensive Summary The bottom-up construction of self-powered artificial cells is significant to understand the energy supply and metabolism of nature cells. Here, we demonstrate an efficient manner to build thylakoid-containing artificial cells, which continuously convert light energy into chemical energy to supply adenosine 5’-triphosphate (ATP) under light illumination. The production of ATP supplies energy to promote the biological enzyme cascade reactions, where glucose is transformed into glucose 6-phosphate (G6P) under the catalysis of hexokinase (HK). G6P was further converted to gluconolactone 6-phosphate (PG) in the presence of 6-phosphate dehydrogenase (G6PDH), meanwhile NADP + was converted to nicotinamide adenine dinucleotide phosphate (NADPH). The self-powered artificial cells were demonstrated to generate ATP and NADPH successively, which provided a way for building more complicated artificial cells.
研究领域:
生物化学
·
细胞生物学
·
合成生物学
·
能量代谢
✓ 已验证
产品关联
DOI
高影响因子
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
·
2025
·Vol. 122
·期 13
·e2419507122
·
IF 9.1
Vincent Mukwaya,Xiaolei Yu,Shuo Yang,Stephen Mann,Hongjing Dou
Liquid–liquid phase separation (LLPS) has been achieved in various cytomimetic (protocell) models, but controlling molecular condensation using noninert crowders to systematically alter protocell function remains challenging. Intracellular ATP levels influence protein–protein interactions, and dysregulation of ATP can alter cellular crowding dynamics, thereby disrupting the normal formation or dissolution of condensates. Here, we develop a membranized protocell model capable of endogenous LLPS and liquid–gel-like phase separation through precise manipulation of intermolecular interactions within semipermeable polysaccharide-based microcapsules (polysaccharidosomes, P-somes), prepared using microtemplate-guided assembly. We demonstrate that intraprotocellular diffusion-mediated LLPS can be extended into the liquid–gel-like domain by the uptake of the biologically active crowder ATP, resulting in a range of modalities dependent on the fine-tuning of molecular condensation. Endogenous enzyme activity in these crowded polysaccharidosomes is enhanced compared to free enzymes in solution, though this enhancement diminishes at higher levels of intraprotocellular condensation. Additionally, increased molecular crowding inhibits intraprotocell DNA strand displacement reactions. Our findings introduce an expedient and optimized approach to the batch construction of membranized protocell models with controllable molecular crowding and functional diversity. Our mix–incubate–wash protocol for inducing endogenous LLPS in membranized protocells offers potential applications in microreactor technology, environmental sensing, and the delivery and sustained release of therapeutics.
研究领域:
生物物理学
·
合成生物学
·
化学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Hong Huang,Huiru Li,Yong Zhang,Xuhan Xia,Ningwen Zhang,Haixin Fan,Longhua Guo,Yongyong Cao,Hu Pan,Ruijie Deng,Yangang Wang,Rodrigo Ledesma-Amaro,Jianguo Xu
Cellular redox homeostasis and energy metabolism in the central nervous system are associated with neurodegenerative diseases. However, their real-time and concurrent monitoring in thick tissues remains challenging. Herein, a single dual-emission two-photon fluorescent probe (named DST ) is designed for the simultaneous tracking of tyrosinase (TYR) and adenosine triphosphate (ATP), thereby enabling the real-time monitoring of both neurocellular redox homeostasis and energy metabolism in brain tissue. The developed DST probe exhibits excellent sensitivity and selectivity toward TYR and ATP, with distinctive responses in the blue and red fluorescence channels being observed without spectra crosstalk. Using this probe, the correlation and regulatory mechanism between TYR and ATP during oxidative stress are uncovered. Additionally, the two-photon nature of this probe allows alterations in the TYR and ATP levels to be monitored across different brain regions in an Alzheimer's disease (AD) mouse model. Notably, a significant decrease in ATP levels is revealed within the somatosensory cortex (S1BF) and caudate putamen brain regions of an AD mouse, alongside an increase in TYR levels within the S1BF and laterodorsal thalamic nucleus brain regions. These findings indicate the potential of applying the spatially resolved regulation of neurocellular redox homeostasis and energy metabolism to treat neurodegenerative diseases.
研究领域:
神经科学
·
分析化学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xin Xu,Peng Luo,Huan Yang,Shuang Pan,Hui Liu,Xiaoli Hu
This paper reports a facile approach for fabricating metal–adenosine triphosphate (ATP) nanoparticles (NPs) by the self-assembly of ATP and metal ions under moderate conditions. Here, Cu-ATP NPs only showed laccase-like activity, but the Fe 3+ -doped Cu-ATP NPs could exhibit peroxidase-like activity, resulting in a decrease in laccase-like activity. Mn 2+ ions functioned as a regulator to balance the enzyme-like activities of CuFe-ATP NPs, endowing the CuMnFe-ATP NPs with both strong laccase- and peroxidase-like activities. Surprisingly, the CuMnFe-ATP NPs also exhibited catalase-like activity under neutral conditions, improving the efficiency of phenol oxidation. X-ray photoelectron spectroscopy (XPS) analysis confirmed that the mixed valence states of the metals in the CuMnFe-ATP NPs accounted for the high catalytic efficiency; also, the large surface area and intrinsic porosity of the NPs were of great importance. The kinetic studies results indicated that the CuMnFe-ATP NPs exhibited rather strong affinities to 3,3,5′,5′-tetramethylbenzidine (TMB) and 2, 4-dichlorophenol (2,4-DP). On the basis of peroxidase-mimic activity, the CuMnFe-ATP NP system was employed to establish a facile colorimetric biosensing method for H 2 O 2 determination, and the detection limit of H 2 O 2 was 0.047 μmol⋅L −1 . These results prove that the enzyme-like activities of Cu-ATP NPs can be modulated via simple metal doping, which may extend to other nanozymes.
研究领域:
生物化学
·
纳米生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ChemistrySelect
·
2023
·Vol. 8
·期 5
·e202204363
·
IF 2.1
Tingting Xu,Panpan Xu,Guilin Xu,Mengyao Liu,Yinggui Zhu
Graphical In the study, Ru(bpy) 3 2+ was used as the luminophore, HEPES buffer was served as the co-reactant, and ATP was added to the system to amplify the luminous signal. It was speculated that ATP acts as an co-reaction accelerator to promote the oxidation of HEPES to HEPES + ⋅, more HEPES⋅ can be generated, which then reacts with oxidized Ru(bpy) 3 3+ to emit stronger ECL signals. Moreover, I - consumes ATP, Ru(bpy) 3 2+ , and HEPES, leading to a decrease in ECL signal. Adenosine triphosphate is an important biological molecule that provides energy and substrate for various cellular biochemical processes,it's ubiquitous in our lives. In this work, a novel electrochemiluminescence (ECL) sensor composed of Ru(bpy) 3 2+ , 2-[4-(2-hydroxyethyl)piperazin-1-yl] ethanesulfonic acid (HEPES) and adenosine 5’-triphosphate disodium salt (ATP) was developed for the first time. In this ECL system, Ru(bpy) 3 2+ was used as the luminophore, HEPES buffer was served as the co-reactant, and ATP was added to the system to amplify the luminous signal to investigate the ECL behavior. Through fluorescence spectroscopy, ECL spectroscopy, and cyclic voltammetry (CV), it can be hypothesized that the possible ECL mechanism is that ATP acts as an advanced co-reaction promoter to accelerate the oxidation of HEPES to HEPES + ⋅, more HEPES⋅ can be generated, which then reacts with oxidized Ru(bpy) 3 3+ to emit stronger ECL signals. Subsequently, the influence of the ATP concentrations and the CV scanning speed was investaged. Moreover, it was observed that I − could quench the ECL intensity, the linear relationship range of I − concentration is from 10 −3 to 10 −6 m , the limit of detection is 3.7×10 −8 m (signal/noise=3), therefore, a new, highly sensitive, and selective ECL approach for I − detection was created.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）