Advanced Composites and Hybrid Materials
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2024
·Vol. 8
·期 1
·1-14
·
IF 23.2
Long Bo,Zhang Qian,Zhang Lintong,Liu Qi,Xing Qiongqiong,Qu Fangfang,Deng Liying,Yan Peng,Wang Liwei,An Meng,Ye Dapeng,Yuan Zhanhui
Trace amounts of antibiotics in water can accumulate in the human body through the food chain, posing significant health risks. Therefore, there is an urgent need to develop simple and effective methods for detecting antibiotics in water. In this study, we prepared electrochemical aptamer sensors based on carbon nanotubes@polystyrene sulfonate-gold nanoparticles/reduced graphene oxide (CNT@PSS-AuNPs/rGO) layered thin films for real-time, on-site detection of ciprofloxacin (CIP) in aquaculture environments, utilizing a portable sensing detection device. The CNT@PSS-AuNPs/rGO layered film offers an excellent specific surface area, providing ample binding sites for the aptamer. The functionalized CNT@PSS-AuNPs enhance the dispersibility and conductivity of the substrate material and increase the surface area of the electrode when loaded with rGO. Under optimal experimental conditions, the developed sensor exhibits a dynamic range from 4 ng/mL to 1.0 × 10 3 ng/mL and a limit of detection of 4 ng/mL (S/N = 3), demonstrating satisfactory sensitivity. The sensor also shows good stability, with a relative standard deviation of less than 1% after 100 repeated measurements. Moreover, when combined with a portable detection platform, CIP levels in aqueous environments can be analyzed intelligently, rapidly, and timely. Our study aims to promote simple and effective detection strategies, potentially extending their practical applications.
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Yachen Li,Ziwen Tang,Yimiao He,Fei Wang,Hui Xu,Fuxiang Zhou,Xinghu Ji,Zhike He
The development of effective glucose monitoring methods is essential for diabetic patients, enabling them to better manage their blood glucose levels and prevent severe complications such as myocardial infarction and renal failure. In this study, we successfully constructed a fluorescent probe based on aminobenzeneboronic acid functionalized CdTe quantum dots (QDs-APBA) and mercaptophenylboronic acid modified gold nanoparticles (MPBA-AuNPs). The fluorescence intensity of QDs-APBA was influenced by the metal enhanced fluorescence (MEF) effect from MPBA-AuNPs, achieving a 2.6-fold enhancement. The fluorescence lifetime demonstrated that the mechanism of MEF was localized surface plasmon resonance (LSPR) instead of intrinsic radiative decay rate effect. Meanwhile, the influences of AuNPs size on the local electric field were analyzed in detail using the Finite-Difference Time-Domain (FDTD) method. These findings were in good agreement with the experimental results. Finally, the assembly has further been applied to glucose detection. Under pH 7.4 conditions, the assembly probe exhibited excellent selectivity and sensitivity for glucose detection with a detection limit of 2.8 μM and a linear range of 3.3–150 μM. This probe allowed for visual detection of glucose concentration changes with the naked eye. Specifically, we detected glucose using alginate hydrogel microspheres embedded with QDs-APBA and MPBA-AuNPs (QD-AuNP MS) and showed the reversible interaction between phenylboronic acid and glucose. This innovative approach not only improves the selectivity and sensitivity of glucose detection but also offers a new strategy for the development of advanced glucose-detecting technologies.
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npj Science of Food
·
2024
·Vol. 8
·期 1
·1-10
·
IF 6.3
Li Hang,Xu Hui,Yao Shuo,Wei Shengnan,Liu Yi,Shi Xuening,Zhao Wei,Zhao Chao
Pathogens detection is a crucial measure in the prevention of foodborne diseases. This study developed a novel multicolor colorimetric assay to visually detect Salmonella Typhimurium ( S. Typhimurium ), by utilizing the etching process of gold nanorods (AuNRs) with TMB 2+ . The strategy involved the construction of nanozyme by assembling magnetic covalent organic framework (MCOF) with aptamer-conjugated AuNPs (Apt-AuNPs), exhibiting remarkable peroxidase-like activity to catalyze the oxidation of TMB/H 2 O 2 and inducing the etching of AuNRs. The presence of S. Typhimurium could inhibit this process, resulting in the generation of vivid colors. The multicolor colorimetric assay could specifically determine S. Typhimurium from 10 2 to 10 8 CFU mL −1 in 60 min with visual detection limit of 10 2 CFU mL −1 , and instrumental detection limit of 2.3 CFU mL −1 . Moreover, detecting S. Typhimurium in chicken, milk, pork and lettuce samples has shown promise in practical applications.
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JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2018
·
IF 1.911
Cheng Zhao,Xiuwen Wu,Guopu Chen,Feng Wang,Jianan Ren
Accelerating wound healing with modified biomaterial has been an attracting field in both material science and medicine. Enhanced cell adhesion could be acquired by improving surface hydrophilicity, which contributes to accelerating wound healing. Chemical reaction has been used for surface modification, but this study used a simple and nontoxic method to improve the hydrophilicity. Polycaprolactone (PCL) scaffold has been regarded as promising material for wound healing while its surface is hydrophobic. Our study demonstrated enhanced hydrophilicity of PCL with AuNPs coating. AuNPs has good biocompatibility and excellent photothermal effect. The coating of AuNPs not only improved the cell adhesion, but also gave PCL the ability to inhibit the growth of bacteria. Animal study showed that the nanocomposites decreased lymphocytes and neutrophils, increased neovascularization and accelerated the abdominal wound healing, which was attributed to improved hydrophilicity and the antibacterial ability. In conclusion, we demonstrated that the nanocomposite could be used as a potential scaffold for cell adhesion and wound healing, and the role of AuNPs was highlighted as a kind of outstanding supplement.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jiaying Zhao,Danqun Huo,Xintong Geng,Jing Bao,Jingzhou Hou,Zhengfan Shui,Huisi Yang,Yanli Qi,Yian Hu,Mei Yang,Changjun Hou
p53 gene is one of the most important anticancer genes and has been recognized as a typical biomarker for the early diagnosis, classification, and prognosis evaluation of cancers. In this work, a novel biosensing strategy integrating MoS 2 -AuNPs modified carbon paper (CP) conductive substrate and enzyme signal amplification technique was developed for p53 gene detection. We constructed an immobilized DNA S1 probe and a biotinylated DNA S3 probe, which could induce the formation of an S1-p53-S3 DNA sandwich complex in the presence of target sequence p53. After conjugating avidin-HRP via biotin-avidin reaction, 3,3’,5,5’-tetramethylbenzidine would be catalyzed for signal amplification in the presence of H 2 O 2 so that the induced electrochemical response would change with proportional to the abundance of p53 gene. The proposed biosensor exhibited two linearities in the range of 1.0 × 10 −15 ∼ 1.0 × 10 -12 M and 1.0 × 10 -12 ∼ 1.0 × 10 -6 M, and the limit of detection (LOD) was 68 fM. No significant interferences were observed during detecting real serum samples. Moreover, it is worth pointing out that the biosensor together with its disposable, low-cost, and stable features can discriminate well between the mutated and wild type p53 gene extracted from tumor cells (wtTP53 in MCF-7 and mutated in SK-BR-3), demonstrating a promising applicability for clinical test.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Xiao-Zhen Feng,Yu-Qi Ge,Jiang-Tao Xu,Zhencheng Chen,Guo-Cheng Han,Zhiliang Jiang
MWCNTs@GO@CS film enhances aptamer binding and sensor stability. • Wide linear range (0.03–100.00 nM) with 0.01 nM LOD. • The sensor exhibits simplicity, accurate for CIP detection in real samples. • It has potential application value in on-site analysis. A highly sensitive electrochemical aptasensor (BSA/Apt/MWCNTs@GO@CS/. AuNPs/SPCE) was fabricated for the efficient detection of ciprofloxacin (CIP). The complementary structural and physicochemical properties of MWCNTs and GO provide a conductive and functionalized platform for aptamer immobilization, while the excellent film-forming property of CS ensures stable and uniform deposition of the nanomaterials on the limited SPCE surface. The sensor displayed excellent linearity by the DPV method from 0.03 to 100.00 nM with a detection limit of 0.01 nmol/L. Spiked recovery tests in lake water and river water achieved 97.98–103.98% recovery with relative standard deviations below 10%, indicating acceptable precision. Moreover, results from chicken samples analyzed by the proposed sensor were consistent with HPLC-MS, with only 0.9% relative error, confirming the sensor's reliability and practical applicability for real-world CIP monitoring. Scheme 1. (A) Fabrication process of the BSA/Apt/MWCNTs@GO@CS/AuNPs/SPCE sensor; (B) The detection process of CIP in real samples. Download: Download high-res image (134KB) Download: Download full-size image
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Green Energy & Environment
·
2017
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IF 0
Kaikai Li,Tifeng Jiao,Ruirui Xing,Guodong Zou,Qianran Zhao,Jingxin Zhou,Lexin Zhang,Qiuming Peng
MXene is a new type of layered two-dimensional transition metal carbide materials differing from graphene, demonstrating intriguing chemical/physical properties. Here the chemical modification of MXene and next fabrication of core–shell MXene–COOH@(PEI/PAA) n composites have been investigated. The obtained MXene-based composites were treated with gold nanoparticles to form MXene–COOH@(PEI/PAA) n @AuNPs nanocomposites, and their catalytic properties for nitro-compounds were studied. The prepared nanocomposites demonstrated good catalytic activity and reproducibility, showing potential applications in composite catalysts and environmental fields.
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ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Liming Chen,Youju Huang,Liping Song,Wuliang Yin,Linxi Hou,Xuqing Liu,Tao Chen
Well-designed 2D materials with ultrathin structures show great potential for humidity-sensing performance owing to their high surface-volume ratio and a great number of exposed atoms on the surface. However, some sensing elements employed for healthcare applications may be considered as potentially risky, such as inflammation, granuloma formation, and carcinogenesis. Herein, we explored biofriendly humidity-sensing characteristics inspired by the great biocompatibility and conductivity of hyperbranched polyethyleneimine-capped gold nanoparticles and cross-linked with polydopamine from the adhesive proteins in mussels. It was successfully employed into two kinds of wearable devices, sports watches and breathing masks, for real-time recording humidity’s fluctuation in expiration and sweat with changes of individual’s crying, laughing, nervous, sleeping, training, and cold states. The wearable devices allow us to monitor individual’s physical activities and emotional states well, suggesting a promising prospect in safe, reusable, long term, and noncontact human health monitoring applications.
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Carbon Letters
·
2023
·Vol. 34
·期 3
·907-915
·
IF 4.5
Yang Jing,Wang Xingyuan,Zhou Tingting,Wei Liangjun,Guo Meiling,Liu Yanan,Sun Xiaoqi,Wang Yanjie
This paper presents the construction and characterization of an amperometric immunosensor based on the graphene/gold nanoparticles (AuNPs/GO) nanocomposite for the detection of the bladder cancer biomarker, apolipoprotein A1 (Apo-A1). The morphological analysis of the AuNPs/GO nanocomposite demonstrated an almost spherical shape of AuNPs and the successful coverage of their surface by graphene oxide. An increased G peak and decreased D peak after the association of AuNPs with GO, implied a reduction in graphene defects. The X-ray photoelectron spectroscopy (XPS) indicated a significant decrease in the quantity of oxygen-containing functional groups in the AuNPs/GO nanocomposite, as compared to the original GO. Furthermore, the developed sensor demonstrated commendable sensitivity and selectivity, with a wide linear range for Apo-A1 detection. Importantly, the immunosensor exhibited remarkable stability over a period of 14 days, signifying its potential for practical applications.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Imamdin Chandio,Suet Cheung,Lei Wu,Mengyang Cui,Yongjian Ai,Qionglin Liang
Early detection of cancer is critical for reducing mortality and improving patient outcomes, yet it remains a challenge due to limitations in existing diagnostic methods. Alpha-fetoprotein (AFP), a glycoprotein predominantly produced during fetal development, has emerged as a vital biomarker for diagnosing cancers such as hepatocellular carcinoma (HCC), germ cell tumours, and yolk sac tumours. However, conventional methods for AFP detection, including immunoassays and enzyme-linked techniques, face challenges such as high costs and limited sensitivity. Here, we have developed a novel label-free electrochemical aptasensor based on a reduced graphene oxide-gold nanoparticle-MXene composite (rGO-AuNPs@MXene). This hybrid material leverages the high conductivity and catalytic activity of AuNPs, the large surface area and stability of rGO, and the exceptional electrochemical properties of MXenes. The rGO-AuNPs@MXene based composite offers high sensitivity, excellent selectivity, and favorable biocompatibility for the detection of AFP. NH 2 -aptamers were immobilized onto the rGO-AuNPs@MXene-modified electrode, enabling precise AFP recognition through specific aptamer-target interactions. The aptasensor demonstrated a broad detection range (10 fg mL −1 to 1 μg mL −1 ), with an ultra-low limit of detection of 13.91 fg mL −1 (S/N = 3). It demonstrated excellent stability, with only a 3.80 % decrease in signal after 8 days of storage, and remarkable reproducibility, showing a relative standard deviation of 1.85 % across five electrodes. These findings emphasize the remarkable efficiency of the rGO-AuNPs@MXene composite in producing highly sensitive and reliable electrochemical aptasensors. This innovation underscores its substantial potential for advancing early cancer detection and improving clinical diagnosis.
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Analytical Methods
·
2026
·
IF 2.6
Jinghan Li,Kemian Liu,Xiaoge Duan,Huajuan Mo,Siyu Liu,Shengda Qin,Liang Luo,Yu Wen,Qiuhua Wang,Hailan Chen
The widespread use of toltrazuril (TOL) as a low-toxicity anticoccidial agent in livestock production necessitates sensitive monitoring of its persistent metabolite, toltrazuril sulfone (TOS), which exhibits hepatotoxic and reproductive risks at subchronic exposure levels. Here, we proposed a novel electrochemical immunosensor for the ultrasensitive detection of TOS in eggs, utilizing a decorated amino-functionalized cerium metal-organic framework-gold nanoparticle (NH₂-Ce-MOF/AuNPs) nanocomposite. The NH₂-Ce-MOF was synthesized via a hydrothermal crystallization of (NH₄)₂Ce(NO₃)₆ and 2-aminoterephthalic acid, exhibiting exceptional stability and an expansive surface area. Compared to conventional electrodes, NH₂-Ce-MOF-modified electrodes promoted AuNPs nucleation and provided more active sites for HAuCl₄ deposition, while also enhancing antibody binding, thereby improving the detection sensitivity of electrochemical immunosensors. Subsequently, electrodeposited HAuCl₄ enhanced both electron transfer kinetics (charge transfer resistance decreased by 68% to a bare electrode) and bioreceptor loading capacity. Electrochemical evaluation demonstrated superior sensor performance, achieving a wide linear range of 0.05 ng/mL–100 ng/mL (R²=0.998) with an ultralow limit of detection of 33.2 pg/mL (S/N=3), representing a 3-fold improvement over conventional enzyme-linked immunosorbent assays. The electrochemical immunosensor exhibited excellent selectivity and reproducibility (relative standard deviation<10%). This platform establishes a robust foundation for TOS residues and other hazardous contaminant monitoring in complex food matrices.
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MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Tianxin Zuo,Hengchang Zhang,Kaidi Sun,Hairong Chen,Caiqin Han
AuNPs@BN@AgNR SERS sensor for ultrasensitive salivary BR/BV detection. • Ultra-low LODs of 5 × 10 −8 M for BR and 1 × 10 −7 M for BV in spiked saliva. • FDTD simulations confirm 5.54-fold electric field enhancement via LSPR coupling. • BN layer enables target enrichment and suppressed non-specific adsorption. Bilirubin (BR) and biliverdin (BV) are non-invasive biomarkers for hepatic function assessment. Their detection in saliva is limited by sub-micromolar concentrations and matrix interference. Conventional methods exhibit limits of detection (LODs) ≥ 10 −6 M. Herein, an AuNPs@BN@AgNR SERS sensor is fabricated via oblique angle deposition, wet chemical etching, and chemical reduction. FDTD simulations demonstrate a 5.54-fold local electric field enhancement in the x–z plane relative to the BN@AgNR bilayer, resulting from LSPR coupling between AuNPs and AgNRs. Experimentally, the substrate achieves enhancement factors up to 4.06 × 10 9 with a spatial relative standard deviation (RSD) of ≤7.00%. The sensor yields LODs of 1 × 10 −12 M (BPE), 2.5 × 10 −8 M (R6G), and 1 × 10 −6 M (CV). In spiked saliva, the sensor exhibits quantifiable concentration ranges of 5 × 10 −8 M to 1 × 10 −4 M for BR and 1 × 10 −7 M to 1 × 10 −4 M for BV, with corresponding LODs of 5 × 10 −8 M and 1 × 10 −7 M. This performance is attributed to BN-mediated electrostatic target enrichment and the suppression of non-specific protein adsorption. This study provides an analytical platform for the non-invasive trace analysis of hepatic biomarkers. Download: Download high-res image (112KB) Download: Download full-size image
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