International Journal of Ophthalmology
·
2025
·Vol. 18
·期 1
·39
·
IF 1.8
Lin-Ping Xue,Hai-Song Feng
AIM To explore the neuroprotective effects of high mobility group box 2 ( HMGB2 ) knockdown on retinal ganglion cells (RGCs) in the retinal ischemia-reperfusion injury (RIRI). METHODS Oxygen-glucose deprivation (OGD)-injured RGCs from postnatal three-day C57BL/6 mice pups and high intraocular pressure (IOP)-induced RIRI mice were used as cellular and animal models of RIRI. The expression of HMGB2 in the retina of RIRI mice and OGD-injured RGCs was detected through reverse transcription-polymerase chain reaction (RT-qPCR) and Western blotting. The effects of HMGB2 silencing on the morphological changes, RGCs survival, and cell apoptosis in mouse retinal tissues were observed through H&E staining, immunofluorescence staining with RNA-binding protein with multiple splicing (RBPMS) antibody, and TUNEL staining, respectively. RGC viability and apoptosis were examined by CCK-8 and flow cytometry assays. The levels of proteins associated with NOD-like receptor thermal protein domain associated protein 3 (NLRP3)-mediated pyroptosis [NLRP3, Caspase-1, GSDMD-N, interleukin (IL)-1β, IL-18] in vivo and in vitro were measured by Western blotting. RESULTS HMGB2 protein and NLRP3 were upregulated in the retina of RIRI mice and OGD-injured RGCs ( P <0.001). The retina was edematous, accompanied by disorganized cell arrangement and decreased thickness of all layers, and obvious vacuoles in ganglion cell layer. HMGB2 silencing alleviated the reduction in total retinal thickness and the severity of retinal tissue damage as well as suppressed RGC loss and retinal cell apoptosis in RIRI mice. OGD-induced RGC apoptosis was ameliorated after downregulation of HMGB2 in vitro . Intravitreal injection of the AAV-sh-HMGB2 and si-HMGB2 resulted in significantly decrease of NLRP3, Caspase-1, GSDMD-N, IL-1β, and IL-18 protein levels in the retinal tissues of RIRI mice and OGD-injured RGCs, respectively (all P <0.001). CONCLUSION HMGB2 knockdown protects against RGC apoptosis and pyroptosis after RIRI through suppressing NLRP3 inflammasome activation.
研究领域:
眼科学
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神经科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2026
·
IF 9.2
Haoran Xu,Jiaqi Zheng,Ze Tao,Yong Tan,Xia Xiao,Chunyu Liu,Xing Teng,Zheng Li,Yi Zhang,Ye Wang,Xin Chen
Thermal processing affects nutrient retention in fruits, yet quantitative in-process monitoring remains challenging. Excitation-emission matrix (EEM) fluorescence combined with principal component analysis (PCA) was used to track changes in nutritional components during apple boiling. Overlapped fluorescence bands were resolved using Gaussian multi-peak empirical function fitting to derive peak descriptors. re regions were screened using minimum redundancy-maximum relevance (mRMR) and χ 2 tests; the synthetic minority over-sampling technique (SMOTE) was used for class balancing. Support vector machine (SVM) achieved 90.84-97.76% accuracy for boiling-stage classification ( κ = 0.9019-0.9761). Partial least squares (PLS) provided the best concentration prediction for both vitamin B 2
( R 2 = 0 . 9734 , root mean square error (RMSE) = 0.0239) and salicylic acid ( R 2 = 0 . 9614 , RMSE = 0.0187). Kinetics showed distinct maxima: salicylic acid (11.588-28.796 ng/mL) peaked at 30-35 min (75-88 °C), while vitamin B 2 (2.164-6.001 ng/mL) peaked at 54-58 min at 100 °C. This peak-shape-aware EEM machine-learning framework supports non-destructive, quantitative process monitoring for nutrient retention during thermal processing.
✓ 已验证
DOI
PubMed
高影响因子
CRYSTENGCOMM
·
2022
·Vol. 24
·期 24
·4399-4407
·
IF 3.756
Delei Liu,Bing Jiao,Haijun Zhang,Shaowei Zhang,Jianghao Liu
By taking advantage of a microwave and molten-salt co-assisted boro/carbothermal reduction method (MSM-BCTR) and cheap raw materials such as HfO2/ZrO2, B4C and amorphous carbon, three types of (HfxZr(1−x))B2 (x = 0.25, 0.50 and 0.75) solid-solution powders with a single-crystalline rod-like morphology, high compositional uniformity, long lengths of 27.8–41.6 μm and high aspect ratios of 10.1–17.2 were successfully synthesized for the first time. And the required temperature conditions of 1100 °C for 20 min were significantly milder than that required by the molten-salt-assisted reduction method even using strong but high-price elemental boron as a reducing agent. These results demonstrated that the specific MSM-BCTR conditions characterized by molten-salt medium and microwave heating were capable of greatly enhancing not only the kinetics but also the thermodynamic favorabilities of the reactions for synthesizing HfB2 and ZrB2 as well as their solid solutions ((HfxZr(1−x))B2). More interestingly, the investigation on the crystalline growth mechanism of (HfxZr1−x)B2 indicated that under the MSM-BCTR conditions, residual amorphous carbon played an important role in enhancing the oriented growth of (HfxZr(1−x))B2, by preferential adsorption onto their {001} crystal planes and therefore greatly promoting corresponding specific surface energies. This study was expected to provide a feasible way of highly-efficiently and cost-effectively synthesizing ultra-high temperature ceramic powders with a highly-crystalline structure and uniform anisotropic morphology, which were favorable for promoting the oxidation resistance and toughness of their sintered products.
研究领域:
材料科学
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陶瓷合成
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产品关联
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2024
·
IF 4.6
Min Li,Zhen-Fang Shang,Zhi-Jing Ni,Hui-Jun Zhao,Qian Lei,Zhao-Jun Wei
Auxenochlorella pyrenoidosa 's industrial utility is closely associated with the concentration of high-value products. This study focuses on the effects of cinnamic acid (CA) and its derivatives, which known as secondary metabolites in higher plants and effective plant growth regulators, on the growth and metabolic profiles of A. pyrenoidosa . It was discovered that CA and its derivatives significantly enhance the biomass of A. pyrenoidosa under conditions of 10 mg/L over 48 h or 100 mg/L over 120 h. Cells treated with 10 mg/L CA exhibited lower oxidative stress and maintained robust metabolic activity. Metabolic pathways, including those for carbohydrates, lipids, small peptides, and cofactors, were stimulated, leading to the accumulation of a wide array of bio-products, notably high-value products such as α-linolenic acid, cis-4,7,10,13,16,19-docosahexaenoic acid (DHA), nicotinamide adenine dinucleotide plus hydrogen (NADH), glutathione, and vitamins B2/B3. This study demonstrates that CA and its derivatives are good nutritional enhancers for cultivating A. pyrenoidosa , highlighting their potential for broad application in biotechnological industries.
研究领域:
生物技术
·
微生物学
·
代谢组学
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产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 11
·2683
·
IF 4.2
Yu Shen,Shihao Wu,Mingming Song,Huiming Zhang,Hong Zhao,Lili Wu,Hongbo Zhao,Hongbin Qiu,Yu Zhang
Polysaccharides have been assessed as a potential natural active component in Chinese herbal medicine with anti-inflammatory properties. However, the complex and indefinite structures of polysaccharides limit their applications. This study explains the structures and anti-inflammatory potentials of three neutral polysaccharides, RIP-A1 (Mw1.8 × 104Da), RIP-B1 (Mw7.4 × 104Da) and RIP-B2 (Mw9.3 × 104Da), which were isolated from the roots ofIsatis indigoticaFort. with sequenced ultrafiltration membrane columns, DEAE-52 and Sephadex G-100. The planar structures and microstructures of RIP-A1, RIP-B1 and RIP-B2 were further determined by HPGPC, GC–MS, methylation analysis, FT-IR, SEM and AFM, in which the structure of RIP-A1 was elucidated in detail using 1D/2D NMR. The Raw 264.7 cells were used for the anti-inflammatory activity in vitro. The results showed that RIP-A1, RIP-B1 and RIP-B2 are all neutral polysaccharides, with RIP-A1 having the smallest Mwand the simplest monosaccharide composition of the three. RIP-A1 is mainly composed of Ara and Gal, except for a small quantity of Rha. Its main structure is covered with glycosidic linkages of T-α-Araf, 1,2-α-Rhap, 1,5-α-Araf, T-β-Galp, 1,2,4-α-Rhap, 1,3,5-α-Arafand 1,6-β-Galpwith 0.33:0.12:1.02:0.09:0.45:11.41:10.23. RIP-A1 significantly inhibited pro-inflammatory cytokines (NO, TNF-α, IL-6 and IL-1β) and increased anti-inflammatory cytokines (IL-4) in LPS-stimulated RAW 264.7 cells. Moreover, RIP-A1 could significantly inhibit the mRNA expression of TNF-α, IL-6 and L-1β. It could also activate IKK, p65 and IκBα (the components of the NF-κB signaling pathway). In conclusion, the above results show the structural characterization and anti-inflammatory potentials of RIP-A1 as an effective natural anti-inflammatory drug.
研究领域:
药理学
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生物化学
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分子生物学
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中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2019
·
IF 4.309
Lijie Tan,Zhidan Zeng,Hongbo Lou,Fei Zhang,Xiehang Chen,Songyi Chen,Yuanyuan Xuan,Fang Peng,Qiaoshi Zeng
As a prototype refractory and hard transition-metal carbide, the stability of zirconium carbide (ZrC) under extreme conditions is critical for its applications. Despite the extensive theoretical studies, few experimental results are available until now. In this work, we carried out a comprehensive experimental study of ZrC in a large unexplored pressure–temperature region (up to ∼150 GPa and ∼2000 K) by combining in situ high-pressure and high-temperature synchrotron radiation X-ray diffraction with various ex situ characterization techniques. Our results demonstrate that ZrC (B1 phase) remains stable up to ∼154 GPa at room temperature or at ∼40 GPa up to 2000 K, rather than undergoing the predicted B1-to-B2 phase transition. However, under high pressure and temperature, ZrC is extremely active when water is present. It would react with even trace amount of absorbed water to form ZrO2 and CH4. Our results clarify the structural and chemical stability of ZrC at extreme conditions, which would guide the applications of ZrC and provide new experimental constraint for theoretical calculations.
研究领域:
材料科学
·
物理学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Huan Guo,Bingzhu Wang,Ruifeng Li,Hongrui Wang,Jixiang Dai,Yong She,Yongzhong Zhao,Jianjun Sha
In order to elucidate the synergistic mechanism of boride and carbide phase in ultrahigh-temperature ceramics (UHTCs) under ablation condition, in this work, the ablation behaviour of (Hf, Ta)B 2 -(Hf, Ta)C-SiC ceramics with different (Hf, Ta)B 2 to (Hf, Ta)C molar ratios was systematically investigated by oxygen-acetylene test under a heat flux of 3.2 MW/m 2 . It was found that the oxides generated from (Hf, Ta)B 2 and (Hf, Ta)C enhanced the matching capacity of oxides with different melting points, being formed a dense ablation layer. When the molar ratio of (Hf, Ta)B 2 to (Hf, Ta)C was 2:1, the ceramic achieved a near-zero ablation rate (0.042 μm/s), which was 1.62 times lower than that of (Hf, Ta)C-SiC ceramics. The mechanism for the enhanced ablation resistance was attributed to the Hf 6 Ta 2 O 17 generated during the ablation reaction, which reduced the viscosity of the ablation products and infiltrated into the pores of ceramics. The formation of such ablation layer could inhibit the impregnation of oxygen and the depletion of easily volatile oxides, including SiO 2 and B 2 O 3 , being formed a multi-element composite ablation layer with self-healing ability, which was helpful for the protection of the substrate from continuous oxidation damage.
研究领域:
材料科学
·
陶瓷与复合材料
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高温材料
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烧蚀物理
✓ 已验证
DOI
高影响因子
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
·
2026
·
IF 8.2
Bingzhu Wang,Wenhao Dou,Huan Guo,Jianjun Sha
To address the challenges of achieving densification at lower sintering temperatures and precise control over the microstructure in UHTCs-SiC, a novel reactive hot-pressing sintering technology was proposed to realize the low-temperature densification of fine and uniform (Zr,Ti)B 2 -(Zr,Ti)C-SiC ceramics, as well as the accurate tailoring of phase compositions. The intrinsic relationship between phase compositions, microstructural tailoring and mechanical performance was revealed. As the molar ratio of (Zr,Ti)C/(Zr,Ti)B 2 decreases, rod-shaped microstructural units (ultra-high temperature borides) progressively develop within the ceramic. These elongated features play a beneficial role in deflecting propagating cracks. When coupled with equiaxed (Zr,Ti)C grains, this combination allows the synergistic strengthening and toughening mechanisms to be fully activated, so the (Zr,Ti)B 2 -(Zr,Ti)C-SiC ceramics with a (Zr,Ti)C/(Zr,Ti)B 2 molar ratio of 1:2 exhibit a flexural strength of 479.2 MPa and a fracture toughness of 3.84 MPa·m 1/2 , which are 62.2 % and 40.7 % higher than those of (Zr,Ti)C-SiC ceramics, respectively.
✓ 已验证
DOI
高影响因子
Crystals
·
2023
·Vol. 14
·期 1
·14
·
IF 2.7
Yufei Zu,Zi Wang,Hongliang Tian,Fan Wu,Lianshen Fu,Jixiang Dai,Jianjun Sha
High-entropy diboride-based (MeB2-based) ceramics are promising high-temperature structural materials because of their excellent mechanical properties, high-temperature stability, and oxidation resistance. In order to achieve low-temperature sintering of the high-entropy ceramics, a novel preparation method of high-entropy (Ti,Zr,Nb,Mo,W)B2-SiC ceramics based on reactive sintering of pre-alloyed solid-solution metals and nonmetals of Si, C, B4C was conducted in the current work. Mechanical alloying behavior of the mixed metal powders, as well as the phase composition, microstructure, mechanical properties, and oxidation behavior of the as-sintered MeB2-SiC ceramic were investigated. The XRD, SEM, and EPMA results indicated that the primary MeB2solid-solution and SiC phases could be successfully formed during reactive sintering at a relatively low temperature of 1650 °C. The as-sintered MeB2-SiC ceramics had a high relative density of 97.8% and high mechanical properties (hardness of 19.74 ± 0.8 GPa, flexure strength of 533 ± 38 MPa, and fracture toughness of 6.01 ± 0.77 MPa·m1/2). Combining the oxidation behavior and microstructure evolution of the oxidation layer, a continuous and relatively dense MeOx-SiO2oxidation layer was gradually formed and covered on the external surface, leading to decelerating oxidation behavior after an oxidation exposure time of 10 min.
研究领域:
材料科学
·
高温材料
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2025
·
IF 14.3
Junjie Xu,Wei Sun,Xiang Xiong,Yi Zeng,Qingbo Wen
Conventional ultra-high-temperature ceramic-modified carbon/carbon composites (C/C-UHTCs) face limitations such as excessive erosion in the gas environment of solid rocket motors (SRMs). In contrast, metal-reinforced C/C-UHTCs exhibit enhanced mechanical properties, thermophysical characteristics, and resistance to high-temperature erosion. These advantages indicate significant potential for metal-reinforced C/C-UHTCs to augment the thrust capabilities of SRMs. This study demonstrated the preparation of (Hf, Zr, Ti)C-(Hf, Zr, Ti)B 2 -W-Cu cermet modified C/C composites using slurry infiltration and reactive melt infiltration at 1400°C. The microstructure, formation mechanisms, mechanical properties, and thermophysical properties of C/C-(Hf, Zr, Ti)C-(Hf, Zr, Ti)B 2 -W-Cu composite materials, as well as the ablation behavior of the composite materials under the SRM environment were compared. The results showed that the linear and mass ablation rates of the C/C-(Hf, Zr, Ti)C-(Hf, Zr, Ti)B 2 -W-Cu composite material decreased by 112.1% and 160.4% (0.074 mm s −1 , 0.086 g s −1 ), respectively, compared with those of the C/C-(Hf, Zr, Ti)C composite material. This could be attributed to the formation of a dense, high-strength W-skeleton composite (Hf, Zr, Ti)O 2 oxide layer during the ablation of the C/C-(Hf, Zr, Ti)C-(Hf, Zr, Ti)B 2 -W-Cu composite, which effectively resisted erosion from high-temperature, high-speed and high-particle-concentration two-phase gas flow. This work presents a novel approach to enhancing the ablation resistance of C/C-UHTCs and holds significant promise for the development of structural materials in SRM environments.
✓ 已验证
DOI
高影响因子
JOURNAL OF THE EUROPEAN CERAMIC SOCIETY
·
2025
·
IF 6.2
Huan Guo,Bingzhu Wang,Ruifeng Li,Xiaohu Li,Jixiang Dai,Yongzhong Zhao,Jianjun Sha
Aiming at the high sintering temperature and densification difficulty in ultrahigh-temperature ceramics, the low-temperature facial and fast preparation of ultrahigh-temperature multi-phases (Hf, Ta)B 2 -(Hf, Ta)C-SiC solid solution ceramics was realized using in-situ reaction combined with hot pressing, where the HfSi 2 , TaSi 2 , B 4 C and C were used as raw materials. The effect of Hf/Ta molar ratio on the microstructural evolution and mechanical properties of ultrahigh-temperature multi-phases ceramics was investigated. The results found that the in-situ reaction and the formation of solid solution phase could promote the densification process. On the other hand, the addition of Ta element induced the formation of high aspect ratio grains with an interlock structure within the ceramic matrix and significantly improved the mechanical properties. When the Hf/Ta molar ratio was 7/3, the multi-phases ceramics exhibited the best mechanical properties, with a hardness of 25.59 GPa, a flexural strength of 556.61 MPa, and a fracture toughness of 5.13 MPa·m 1/2 . The enhanced mechanical properties were attributed to the synergistic effect of multiple strengthening and toughening mechanism, including grain bridging, crack bifurcation and grain pullout induced by high aspect ratio (Hf, Ta)B 2 grains.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2024
·
IF 6
Mengting Lai,Hongzhu Chen,Xiaozhen Liu,Fuxiang Li,Fengyuan Liu,Yuting Li,Jingkun Yan,Li Lin
The aim of this study was to reveal the phenolic profile of mangosteen ( Garcinia mangostana L. ) pericarp and investigate their inhibitory effects on α-amylase and α-glucosidase. Mangosteen pericarp polyphenols (MPP) obtained by acetone extraction were fractionated with gradient ethanol elution. A total of twenty nine phenolic chemicals were found and quantified by UPLC-Q-TOF-MS/MS. MPP eluted by the 60% ethanol solution (MPP 60% ) showed the highest total phenolic content. Moreover, MPP 60% displayed the greatest inhibition activities on α-amylase and α-glycosidase by competitive and mixed inhibitions. Mechanistically, the inhibitory effects of MPP 60% on α-amylase and α-glucosidase involved the changes of the secondary structure as well as the static fluorescence quenching of these enzymes. Pearson correlation coefficients revealed that proanthocyanidin B2, quercetin glucoside, and mangostain contributed most to inhibiting the activities of α-amylase and α-glucosidase. This study indicated that polyphenols from mangosteen pericarps can be developed into potential inhibitors of α-amylase and α-glucosidase, offering a viable strategy for the valorization of mangosteen pericarps.
研究领域:
食品科学
·
药理学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）