CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Pengfei Wei,Zuoying Yuan,Wei Jing,Yiqian Huang,Qing Cai,Binbin Guan,Zihao Liu,Xu Zhang,Jianping Mao,Dafu Chen,Xiaoping Yang
Alendronate, a nitrogen-containing bisphosphate, is commonly used for the treatment of osteoporosis, showing the potential in enhancing osteogenesis. To improve the osteo-promoting effect of injectable biomineralized microspheres for bone tissue engineering applications, in this study, alendronate was incorporated onto the microspheres during their biomineralization procedure in simulated body fluid (SBF). For the purpose, the microspheres were made of biodegradable poly( l -lactide)-poly(ethylene glycol) block copolymer, which had rough surface with gelatin coating via the (W 1 /O)/W 2 emulsion preparation method. Different amounts (25 μM, 50 μM or 100 μM) of alendronate were dissolved in the 5-times SBF and incorporated as calcium minerals depositing onto the microspheres, and thus displaying different release behaviors of alendronate. The cell culture results using bone marrow mesenchymal stromal cells (BMSCs) demonstrated that the microspheres prepared from the 50 μM of alendronate had the strongest promotion on osteogenic differentiation, while displayed insignificant adverse effect on cell proliferation and viability. By adding Noggin (an inhibitor for bone morphogenetic protein (BMP)) into the culture systems, it was proposed that BMP signaling pathway was one of the possible pathways for alendronate playing its activating effect. In vivo evaluation was conducted by injecting the alendronate-loaded microspheres subcutaneously into rabbit back, in which, histological and immunohistological analysis were done to assess ectopic neovascularization and osteogenesis. Promisingly, the potential of biomineralized microspheres in promoting osteogenesis was significantly strengthened with the incorporation of alendronate. Therefore, a proper application of alendronate in bone repairing materials can be an effective strategy targeting bone regeneration.
研究领域:
生物医学工程
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组织工程
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药剂学
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细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
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2016
·
IF 8.387
Zhentao Man,Xiaoqing Hu,Zhenlong Liu,Hongjie Huang,Qingyang Meng,Xin Zhang,Linghui Dai,Jiying Zhang,Xin Fu,Xiaoning Duan,Chunyan Zhou,Yingfang Ao
Cartilage tissue engineering is the hotspot of cartilage repair. The allogenic chondrocytes appear to be a promising source of seed cells in cartilage tissue engineering. In this study, we aimed to transplant allogenic chondrocytes with chitosan hydrogel (CS)-demineralized bone matrix (DBM) hybrid scaffold (CS/DBM) to repair rabbit cartilage injury with one-step operation. After the CS/DBM scaffold was successfully fabricated, it showed that the porous CS filled the large pores of DBM, which improved the distribution of seed cells in the CS/DBM scaffold. The allogenic chondrocytes at second passage were transplanted with different scaffolds to repair rabbit cartilage injury. Twenty-four weeks after surgery, the cartilage defect in the CS/DBM group was successfully filled as shown by MRI. Moreover, the histological score of CS/DBM group was significantly higher than that of the other groups. On the aspect of biomechanical property , the regenerated cartilage in the CS/DBM group were superior to those in the other groups as determined by nanoindentation . Meanwhile, no obvious inflammatory response was observed after the transplantation of allogenic chondrocytes at 24 weeks post-surgery. Furtherly, gene expression profile for cells within the repair tissue was compared with the allogenic chondrocytes before transplantation using Agilent microarray and RT-qPCR. The results showed that some genes beneficial to cartilage regeneration, such as BMP-7, HGF , and IGF-1, were upregulated one month after transplantation. Consequently, our study demonstrated that the transplantation of allogenic chondrocytes with CS/DBM scaffold successfully repaired rabbit cartilage injury with only one-step operation, thereby providing new insights into cartilage tissue engineering.
研究领域:
组织工程
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骨科
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 24
·20202-20210
·
IF 3.289
Tianlin Gao,Weiwei Cui,Zongliang Wang,Yu Wang,Ya Liu,Ponnurengam Sivakumar Malliappan,Yoshihiro Ito,Peibiao Zhang
Lack of bioactivity has seriously restricted the development of biodegradable implants in bone tissue engineering. In this study, Bone Morphogenic Protein 2 (BMP-2) was photo-immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA composites via photo-reactive gelatin (Az-Gel). The results demonstrate that the Az-Gel facilitates efficient and long-term immobilization of peptides on the surface of PLGA. The immobilization of Az-Gel enhanced the adhesion and proliferation of Mouse Adipose-Derived Stem Cells (ADSCs), while HA incorporation facilitated cellular osteodifferentiation. The immobilization of BMP-2 induced the osteogenesis of the ADSCs, indicated by alkaline phosphatase activity, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and mineralization on the deposited substrates. Furthermore, the surface immobilization of BMP-2 could not only reduce the amount of growth factor, but also have a long-term osteoinductive effect. Therefore, it is considered that the surface modification method has a great potential for the enhancement of osteointegration of biodegradable bone implants.
研究领域:
组织工程
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生物材料科学
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干细胞研究
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Hewei Wei,Ting Zhao,Xinglong Liu,Qiteng Ding,Shuai Zhang,Jiali Yang,Ning Wang,Lifeng Zhang,Guodong Chai,Guofeng Xia,Anning Li,Chuanbo Ding,Wencong Liu
To solve the difficulty of natural healing in patients with severe bone defects, and the challenges of insufficient autologous bone graft material and large incisions in conventional surgery, we were designed an intelligent co-regulated hydrogel system with NIR response. The goal is to be able to prevent inflammation and bacterial adhesion, thereby promoting repair and development of the bone defect area. The hydrogel system consisted of hyaluronic acid, carboxymethyl chitosan and Pht-loaded PDA-encapsulated ZIF-8 NPs (Pht@ZIF-8@PDA) by Schiff base reaction. Compared to high-temperature photothermal therapy (PTT), which destroyed surrounding healthy cells, the present study was based on a new strategy of mild photothermal therapy (42.5 ± 1.2 °C) and leaded to a better promotion of osteogenesis. NIR activated the photothermal properties of PDA in scaffolds, intelligently released Pht and Zn 2+ , inhibited Escherichia coli and Staphylococcus aureus (inhibition rate ≥ 96 %), and promoted bone regeneration in the rat model. Meanwhile, it could promote bone regeneration by upregulating osteogenic factors and stimulating the expression of proteins related to BMP/SMAD signaling pathway. The research highlights the potential applications of synergistically modulated hydrogels, which opens up a new application path for this material in bone tissue engineering, regenerative medicine, and other related fields.
研究领域:
生物医学工程
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组织工程
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再生医学
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材料科学
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药学
✓ 已验证
产品关联
DOI
高影响因子
Applied Materials Today
·
2024
·
IF 7.2
Zhaohui Dang,Weihua Huang,Xiayu Cai,Jiandong Ye,Weikang Xu
Most bone filler materials currently achieve bone regeneration by mimicking the natural bone extracellular matrix. However, it is difficult for these materials to replicate the structural functions and bioactivities, including immunomodulation, of natural bone perfectly to reduce inflammation and promote bone regeneration synergistically. Repairing bone defects with scaffolds using a decellularised extracellular matrix (dECM) as a matrix material is an important clinical application and research direction. Here, we processed bovine cancellous bone via an optimised combination of decellularisation methods and preferred dECM, which has the shortest processing time and lowest immunogenicity. Hexagonal mesoporous silica (HMS)/poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with bone morphogenetic protein-2 (BMP-2) were prepared using the complex emulsion method. The HMS/PLGA microspheres had longer cytokine release periods than did the separate HMS and PLGA microspheres. Composite BMP-2/HMS/PLGA microspheres were used to prepare dual-loaded cytokine scaffolds with bone immunomodulatory capacity, which were prepared from composite BMP-2/HMS/PLGA microspheres to increase the osteogenic activity of the dECM and to adsorb interleukin-4 (IL-4) on the surface of the scaffolds. The results showed that the dECM had good cytocompatibility and mechanical strength, and the composite microspheres and IL-4 further endowed the dECM with an ordered spatiotemporally controlled release function, which could release BMP-2 for more than 4 months in the long term and release IL-4 for approximately 10 days in the short term. The composite scaffold not only effectively promoted the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) but also immunomodulated the M1 to M2 polarisation of macrophages (MPs) and mediated the M2 polarisation of MPs, which in turn promoted the osteogenic differentiation of BMSCs, creating a favourable immune microenvironment for bone regeneration. In vivo, the dual drug-loaded scaffolds also exhibited good biocompatibility and significantly superior immunomodulatory bone-enhancing properties compared with those of the other groups. In summary, the combination of dECM scaffolds with cytokine-carrying microspheres and immunomodulatory factors can promote the orderly spatiotemporal release of cytokines, which significantly enhances the bone regeneration and repair effects of dECM scaffolds and is a promising bone filler material for clinical application.
研究领域:
组织工程
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再生医学
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生物材料
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免疫学
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骨生物学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Zewen Shi,Fang Yang,Qian Pang,Yiwei Hu,Haijian Wu,Xueqiang Yu,Xianjun Chen,Lin Shi,Bowen Wen,Rui Xu,Ruixia Hou,Dan Liu,Qingjiang Pang,Yabin Zhu
With the development of minimally invasive orthopedics, injectable materials for bone repair are attracted more attention, especially for those wound with a small external mouth and sizeable internal cavity. In this work, the hydrogel with features of thermo-responsiveness, degradability and injectability was designed and fabricated. The hydrogel, named as FHCS, is composed of Pluronic F-127 (F127) loaded with carboxymethyl chitosan/sodium alginate nanoparticles (nCS) and nanohydroxyapatite (nHA). The hydrogel FHCS was non-toxic and good hemocompatible. It can enhance the ALP activity and extracellular matrix calcification of MC3T3-E1 due to the chitosan-based nanoparticle components (nCS). Moreover, FHCS-5 (containing 5 mg/mL nCS) showed relative high expression of osteogenic genes and protein markers. Osteal regeneration was observed treated by FHCS-5 hydrogel in a critical-size rat calvarial bone defect model. CT scanning showed that the whole defect was basically covered by new bone after FHCS-5 hydrogel. The results of H&E staining and Masson's trichrome staining on histological sections further confirmed that FHCS-5 hydrogel promoted new osteal formation and maturation, which up regulated the osteogenic related genes and proteins of ALP, OCN, OPN through BMP/Smad signaling pathway. Hence, this study suggests that FHCS-5 hydrogels have a promising application for non-loading bone regeneration.
研究领域:
生物医学工程
·
材料科学
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骨科
·
组织工程
✓ 已验证
DOI
PubMed
高影响因子
Advanced Science
·
2023
·Vol. 10
·期 33
·2302622
·
IF 15.1
Yifan Ma,Lili Sun,Jingjing Zhang,Chi-ling Chiang,Junjie Pan,Xinyu Wang,Kwang Joo Kwak,Hong Li,Renliang Zhao,Xilal Y. Rima,Chi Zhang,Anan Zhang,Yutong Liu,Zirui He,Derek Hansford,Eduardo Reategui,Changsheng Liu,Andrew S. Lee,Yuan Yuan,Ly James Lee
Regenerative medicine in tissue engineering often relies on stem cells and specific growth factors at a supraphysiological dose. These approaches are costly and may cause severe side effects. Herein, therapeutic small extracellular vesicles (t-sEVs) endogenously loaded with a cocktail of human vascular endothelial growth factor A (VEGF-A) and human bone morphogenetic protein 2 (BMP-2) mRNAs within a customized injectable PEGylated poly (glycerol sebacate) acrylate (PEGS-A) hydrogel for bone regeneration in rats with challenging femur critical-size defects are introduced. Abundant t-sEVs are produced by a facile cellular nanoelectroporation system based on a commercially available track-etched membrane (TM-nanoEP) to deliver plasmid DNAs to human adipose-derived mesenchymal stem cells (hAdMSCs). Upregulated microRNAs associated with the therapeutic mRNAs are enriched in t-sEVs for enhanced angiogenic–osteogenic regeneration. Localized and controlled release of t-sEVs within the PEGS-A hydrogel leads to the retention of therapeutics in the defect site for highly efficient bone regeneration with minimal low accumulation in other organs.
研究领域:
再生医学
·
组织工程
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干细胞生物学
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骨修复
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF PHYSICS
·
2021
·Vol. 23
·期 3
·033019
·
IF 3.732
Bingsuo Zou,Lipeng Hou,Ye Tian,Junbo Han,Hui Peng,Xiongtao Yang,Lijie Shi
The magnetic excitons in diluted magnetic semiconductor (DMS) have varied formats due to the inhomogeneous phases out of doping concentration and/or structural relaxations or defects. Here the high quality cobalt-doped zinc blende ZnSe nanoribbons (NRs) were synthesized, showing bright and color-variable emissions from blue, yellow to a little mixed white colors. Their power and temperature dependent micro-photoluminescence (PL) spectra have been obtained in which two emission bands, one magnetic exciton band near the band-edge and a Co2+ high-level d–d transition emission band at 550 nm out of their ferromagnetic (FM) coupled aggregates in ZnSe lattice, both bands could also be reflected by a nonlinear optical absorption enhancement. The easy formed stacking fault defects in a chemical vapor deposition (CVD) grown ZnSe zincblende NR took part in the above optical processes out of magnetic polaronic excitons (PXs). The femtosecond (fs) laser pulse pumping on single ZnSe:Co NR produces obvious lasing behavior but with profile of a complicated magnetic exciton interactions with indication of a crossover from collective exciton magnetic polarons (EMP) to bound magnetic polaron (BMP) scattering in Co doped ZnSe NR. These findings indicate the complication of the magnetic coupling natures in varied DMS structures, whose optical properties have been found to be highly nonlinear, due to the involvement of the spin–spin, spin–exciton and spin–phonon interactions, verified by the theoretic calculation in Yang X-T et al (2019 Interstitial Zn-modulated ferromagnetism in Co-doped ZnSe Mater. Res. Express 6 106121).
研究领域:
材料科学
·
光学
·
半导体物理
·
纳米技术
·
磁学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）