Small
·
2024
·
IF 13
Sipin Zhu,Qiuji Wu,Yibo Ying,Yuqin Mao,Wenjie Lu,Jie Xu,Xiong Cai,Huacheng He,Jiang Wu
Spinal cord injury (SCI) is a severe clinical disease usually accompanied by activated glial scar, neuronal axon rupture, and disabled motor function. To mimic the microenvironment of the SCI injury site, a hydrogel system with a comparable mechanical property to the spinal cord is desirable. Therefore, a novel elastic bovine serum albumin (BSA) hydrogel is fabricated with excellent adhesive, injectable, and biocompatible properties. The hydrogel is used to deliver paclitaxel (PTX) together with basic fibroblast growth factor (bFGF) to inhibit glial scar formation as well as promote axon regeneration and motor function for SCI repair. Due to the specific interaction of BSA with both drugs, bFGF, and PTX can be controllably released from the hydrogel system to achieve an effective concentration at the wound site during the SCI regeneration process. Moreover, benefiting from the combination of PTX and bFGF, this bFGF/PTX@BSA system significantly aided axon repair by promoting the elongation of axons across the glial scar with reduced reactive astrocyte secretion. In addition, remarkable anti-apoptosis of nerve cells is evident with the bFGF/PTX@BSA system. Subsequently, this multi-functionalized drug system significantly improved the motor function of the rats after SCI. These results reveal that bFGF/PTX@BSA is an ideal functionalized material for nerve repair in SCI.
研究领域:
神经科学
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2018
·Vol. 29
·期 8
·085101
·
IF 3.404
Xi-Xi Ma,Han Gao,Ya-Xuan Zhang,Yi-Yang Jia,Chen Li,Si-Yuan Zhou,Bang-Le Zhang
Non-viral nanovectors have attracted much attention owing to their ability to condense genetic materials and their ease of modification. However, their poor stability, low biocompatibility and gene degradation in endosomes or lysosomes has significantly hampered their application in vivo and in the clinic. In an attempt to overcome these difficulties a series of bovine serum albumin (BSA)–calcium phosphate (CaP) nanoparticles were constructed. The CaP condenses with DNA to form nanocomplexes coated with a biomimetic corona of BSA. Such complexes may retain the inherent endocytosis profile of BSA, with improved biocompatibility. In particular the transgene performance may be enhanced by stimulating the cellular uptake pathway via caveolae-mediated endocytosis. Two methods were employed to construct and optimize the formulation of BSA–CaP nanomaterials. The optimized BSA–CaP-50-M2 nanoparticles prepared by our second method exhibited good stability, negligible cytotoxicity and enhanced transgene performance with long-term expression for 72 h in vivo even with a single dose. Determination of the cellular uptake pathway and Western blot revealed that cellular uptake of the designed BSA–CaP-50-M2 nanoparticles was mainly via caveolae-mediated endocytosis in a non-degradative pathway in which the biomimetic uptake profile of BSA was retained.
研究领域:
纳米技术
·
生物医学工程
·
分子生物学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2023
·
IF 5
Yun Liu,Qian Lan,Jinxiu Liu,Yaxin Shi,Quanxin Wu,Qian Wang,Shuang Yang,Fan Cheng
To avoid the occurrence of antibiotic resistance and improve bactericidal effect, a multifunctional antibacterial nanoagent BCPP was successfully constructed for targeted photodynamic/photothermal combination therapy (PDT/PTT). The prepared BCPP consists of protoporphyrin (PpIX) as the photosensitizer , CuS nanoparticles as the photothermal agent, phenylboric acid (PBA) as the bacteria-targeting segment and bovine serum albumin (BSA) as the colloid stabilizer. Afterwards, its photodynamic/photothermal capacities, bacteria-targeting effect and bactericidal efficiency were intensively investigated. Experimental results confirmed its good singlet oxygen generation ability and high photothermal conversion efficiency. In addition, BCPP displayed good bacteria-targeting properties for both S. aureus and E. coli . Moreover, in vitro antibacterial assays proved its enhanced therapeutic efficiency through combined antibacterial PDT/PTT owing to PBA-mediated bacteria-targeting function. Finally, in vitro assays revealed its good hemocompatibility and low cytotoxicity. To sum up, our study offered a convenient method to build non-antibiotic antibacterial nanoagents for targeted PDT/PTT, and the prepared bactericidal is a promising drug candidate suitable for clinical trials in the future.
研究领域:
纳米技术
·
光疗
·
抗菌剂
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Dong-Xu Tang,Kun Liu,Jin-Yu Yang,Zhi-Jun Wang,Li-Li Fu,Xiao-Jie Yang,Yue-Sheng Li,Bin Huang,Yi Liu
The process of diabetic wound healing was influenced by the excessive proliferation of reactive oxygen species (ROS). Therefore, in the process of healing diabetic wounds, it was crucial to removing ROS. This study designed composited nanoparticles: KBP, consisted by Konjac glucomannan, bovine serum albumin, and Prussian blue. Then they were embedded in Konjac glucomannan and hydroxypropyl trimethylammonium chloride chitosan composite hydrogel (KH), The KBP@KH hydrogel finally achieved excellent efficacy in diabetic wound healing. The in vitro and in vivo experiments demonstrated that KPB nanoparticles exhibited favorable ROS scavenging capability and biosafety. The KBP@KH hydrogel not only effectively eliminated ROS from diabetic wounds, but also exhibited excellent wound adaptability. The KBP@KH hydrogel facilitated angiogenesis and suppressed the production of inflammatory factors. Overall, the KBP@KH hydrogel dressing was characterized by its user-friendly nature, safety, and high efficiency.
研究领域:
材料科学
·
生物医学工程
·
伤口愈合
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2020
·
IF 7.098
Fuqing Ma,Chaojie Cai,Xinzhen Zhao,Hilal Ahmad,Changkun Liu
A novel thin-film nanocomposite (TFN) forward osmosis (FO) membrane was fabricated by loading bovine serum albumin (BSA)/gold nanoparticles (GNPs) on the surface of a cockscomb-like nylon supporting membrane. The influence of the embedded BSA/GNPs on surface morphology , surface roughness , surface charge, separation, and FO performance of the TFN-FO membrane was investigated. The water flux of the BSA/GNPs-modified FO membrane could be increased to 55.2 ± 5.4 LMH, and the J s /J w value was substantially decreased to 0.034 g/L, indicating a high selectivity of the modified membrane. BSA/GNPs located between the top of the nylon supporting membrane and the bottom of the polyamide (PA) skin layer provided passage for water transport and increased the roughness of the PA layer, thus enhancing the effective area on which the osmotic pressure could act. In addition, BSA/GNPs located at the bottom surface of the nylon supporting membrane provided a nearly electroneutral surface in the solution and alleviated dilutive concentration polarization . In summary, choosing a nylon membrane with a special cockscomb-like surface structure as the supporting layer to load BSA/GNPs nanoparticles proved to be a creative and effective nano-modification method for fabricating high-performance TFN-FO membranes.
研究领域:
材料科学
·
膜技术
·
纳米技术
·
水处理
✓ 已验证
产品关联
DOI
高影响因子
DALTON TRANSACTIONS
·
2017
·Vol. 46
·期 7
·2204-2213
·
IF 4.029
Lanfang Ren,Xijian Liu,Qian Wang,Lijuan Zhang,Guoying Deng,Feng Zhou,Jie Lu
In this study, a multifunctional nano-drug (PTX-loaded Cys-Fe3O4/CuS@BSA nanocomposite), which performs well in hydrophobic drug delivery, magnetic resonance imaging (MRI) and chemo-photothermal combination therapy, has been successfully synthesized via a facile method with several biocompatible agents. The characterization verified that our as-obtained nanocarrier has a preferable average diameter of about 300 nm for cancer therapy, shows a great MRI ability and magnetism performance, and exhibits a favorable in vitro and in vivo photothermal therapeutic efficacy by introducing CuS, and the sustained release of chemotherapeutics could be controlled by pH value and near-infrared laser irradiation has further enhanced the therapeutic performance. Overall, the excellent synergistic effect to inhibit tumor growth effectively realized via imaging-guided combinational chemo-photothermal therapy in the present work, highlights the promise of conquering the limitations of tumor treatments.
研究领域:
生物医学工程
·
癌症治疗
·
纳米技术
·
分子影像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Ziliang Zheng,Rong Dai,Zhuo Jia,Xiaorong Yang,Yufei Qin,Shuo Rong,Xiaoyang Peng,Xianmei Xie,Yanyan Wang,Ruiping Zhang
Stimuli-responsive silica nanoparticles are an attractive therapeutic agent for effective tumor ablation, but the responsiveness of silica nanoagents is limited by intrastimulation level and silica framework structure. Herein, a biodegradable hollow SiO2-based nanosystem (Ag2S-GOx@BHS NYs) is developed by a novel one-step dual-template (bovine serum albumin (BSA) and cetyltrimethylammonium bromide (CTAB)) synthetic strategy for image-guided therapy. The Ag2S-GOx@BHS NYs can be specifically activated in the tumor microenvironment via a self-feedback mechanism to achieve reactive oxygen species (ROS)-induced multistep therapy. In response to the inherent acidity and H2O2 at the tumor sites, Ag2S-GOx@BHS would accelerate the structural degradation while releasing glucose oxidase (GOx), which could efficiently deplete intratumoral glucose to copious amounts of gluconic acid and H2O2. More importantly, the sufficient H2O2 not only acts as a reactant to generate Ag+ from Ag2S for metal-ion therapy and improves the oxidative stress but also combines with gluconic acid results in the self-accelerating degradation process. Moreover, the released Ag2S nanoparticles can help the Ag2S-GOx@BHS NYs realize the second near-infrared window fluorescence (NIR-II FL) and photoacoustic (PA) imaging-guided precise photothermal therapy (PTT). Taken together, the development of a self-feedback nanosystem may open up a new dimension for a highly effective multistep tumor therapy.
研究领域:
纳米技术
·
癌症治疗
·
材料科学
·
生物医学工程
·
诊疗学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Huaiqi Shi,Junsong Yue,Muhammad Saad Ahmed,Xiaochen Liu,Min Tu,Yunfei Shen,Yiying Hu,Liru Zhang,Jingyun Zhao,Wenjun Shao,Zhansheng Wu
Ginsenoside Rh2 is a rare but pharmacologically potent component of ginseng, which is known for its immunomodulatory, anti-tumor, and antiaging effects. However, its low natural abundance and the instability of free enzymes used in its biosynthesis hinder large scale production. To address these challenges, a magnetic nanocomposite nickel-based metal organic framework (Ni-MOF) via a mild, bovine serum albumin (BSA)-templated synthesis method was developed. This MOF was engineered to enable simultaneous purification and co-immobilization of recombinant glycosyltransferase (UGT) and sucrose synthase (SUSy), forming a dual-enzyme catalyst system BSA-Trypsin-Fe 3 O 4 /MOF@UGT&SUSy BT-Fe 3 O 4 /MOF@UGT&SUSy for the biotransformation of ginsenoside Rh2. The immobilized enzyme system BSA-Trypsin-Fe 3 O 4 /MOF (BT-Fe 3 O 4 /MOF) demonstrated high enzyme loading 176.39 mg/g and enhanced thermal, pH, and storage stability compared to free enzymes. The co-immobilized UGT-SUSy system also facilitated in situ regeneration of UDP-glucose, significantly improving catalytic efficiency. Under optimized conditions, the system achieved a Rh2 yield of 80.67 μg/mL and retained 58.01 % of the initial activity after 12 repeated cycles. This work not only highlights the potential of BT-Fe 3 O 4 /MOF in sustainable and efficient enzymatic catalysis but also offers a sustainable and scalable approach for the enzymatic synthesis of high-value nutraceuticals.
研究领域:
生物催化
·
酶工程
·
纳米技术
·
医药生物技术
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Zhenzhong Zhang,Shaoguo Ru,Jun Wang,Dong Wang,Lingchao Zhao,Hayan Teng,Zhikuang Dai,Wenzhe Zhang,Ailing Yang
Vitellogenin (Vtg) is a biomarker for environmental estrogens, and its sensitive detection method is vital for the detection of weak estrogenic activity. This study aimed to develop sensitive electrochemical immunosensors for marine medaka ( Oryzias melastigma ) Vtg based on novel nanomaterials. Firstly, Cu 2 O–BSA nanoparticles (NPs) with a diameter of 120−150 nm was synthesized, and monoclonal antibody against lipovitellin (anti-Lv mAb) was prepared. Then two simple and efficient label-free immunosensors for the detection of Vtg were established using Cu 2 O–BSA NPs and gold nanoparticle (Au NPs) as signal amplification elements and anti-Lv mAb as the detection probe. These two immunosensors have good sensitivity, selectivity, reproducibility for Vtg quantification, and are easy to use. Moreover, the BSA/Anti-Lv mAb/Au/Cu 2 O–BSA/GCE immunosensor exhibited a linear range from 0.128 to 5 × 10 4 pg/mL, with the lowest detection limit (LOD) of 0.09 pg/mL, which is considerably lower than those of the previously reported Vtg detection techniques. Finally, the performance of the immunosensor was evaluated by quantifying Vtg concentrations in plasma of male marine medaka exposed three estrogenic bisphenols. The results demonstrated that the BSA/Anti-Lv mAb/Au/Cu 2 O–BSA/GCE immunosensor was an easily-operated, ultrasensitive and accurate detection method for weak estrogenic activity.
研究领域:
环境科学
·
分析化学
·
生物传感器
·
海洋生物学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Shijiao Fan,Zechuan Lu,Zhenning Yan,Liuyang Hu
In this paper, the interaction of three berberine mid-chain fatty acid salts ([BBR][FAs]), viz. berberine caproate ([BBR][CAP]), berberine heptylate ([BBR][HEP]) and berberine octoate ([BBR][OCT]), with bovine serum albumin (BSA) was studied by means of UV–visible absorption spectroscopy , fluorescence spectroscopy, fourier transform infrared spectroscopy (FT-IR) and molecular docking techniques. Fluorescence experiments revealed that three berberine salts quench the fluorescence of BSA by static quenching mechanism resulted from a stable [BBR][FAs]-BSA complex formation. The stoichiometric numbers of [BBR][FAs]-BSA complexes were found to be 1:1. Synchronous and three-dimensional fluorescence spectra as well as FT-IR demonstrated that the binding of [BBR][FAs] altered the microenvironment and conformation of BSA. The binding average distance from [BBR][FAs] to BSA (3.2–3.5 nm) was determined according to Förster energy transfer theory. Site probe investigation showed that [BBR][FAs] bound to BSA active site I (sub-domain IIA). The binding promotes the esterase-like activity of BSA. The molecular docking results confirmed the fluorescence competition findings and provided the type of binding forces. Furthermore, the relationship between the anionic chain length of [BBR][FAs] and the interaction was explored, and the positive correlation was found.
研究领域:
药物化学
·
生物物理化学
·
分子生物学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Lu Lai,Xiao-Qian Wei,Wei-Hua Huang,Ping Mei,Zhao-Hua Ren,Yi Liu
The effects of carbon quantum dots (CQDs) on the dynamic properties of bovine serum albumin (BSA) were investigated using pendant drop profile analysis method. Moreover, the effects of CQDs on the competitive adsorption of BSA and dipalmitoyl phosphatidylcholine (DPPC) were examined. CQDs reduce the fluorescence intensity of BSA and cause a red shift in fluorescence emission . The quenching constant at pH 4.3 is almost twice as large as that of the value obtained at pH 6.0. A small amount of CQDs does not influence the dynamic surface adsorption properties of BSA molecules. As the CQD concentration increases, a gradual increase in adsorption rate of BSA molecules is observed. Moreover, the addition of CQDs results in a significant transition of kinetic dependencies of surface elasticity of BSA solution when the CQD concentration exceeds a critical value. The appearance of the maximum surface elasticity value is probably attributed to the formation of tails and loops. When the dynamic surface properties are dominated by BSA molecules, the effects of CQDs on the surface properties of BSA/DPPC mixture are similar to those of BSA alone. However, when the surface film mainly consists of DPPC, CQDs can obviously change the interfacial properties of DPPC monolayer.
研究领域:
生物物理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Yuliang Cheng,Fan Zhang,Yajie Zhao,Jiannan Chen,Weirong Yao,He Qian
By using crawfish shells as the precursor and hydrothermal synthesis, Bovine serum albumin doped carbon dots (BSA@CDs) were prepared without excessive chemical reagents. The relationship between the fluorescence properties of different BSA@CDs and BSA amount was investigated by various characterization techniques. When the amount of BSA added was 30 %, the prepared BSA@CDs’ quantum yield (QY) reached 25.01 %, which was the highest. Inner Filter Effect (IFE) suggested that Cr (VI) can selectively quench the fluorescence of BSA@CDs. Cr (VI) can be reduced to Cr (III) by Hydroquinone (HQ), thus recovering the fluorescence. Accordingly, using BSA@CDs as a probe, a “turn-on” fluorescence sensor applied in HQ determination was constructed. The linear range was 10–200 µmol/L and limit of detection (LOD) was 0.18 µmol/L. Further, it has been employed to the determination of HQ in both crawfish tail meat and aquaculture water with good performance.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）