INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2022
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IF 8.025
Fengshu Li,Xin Hu,Liying Qin,Haoshuai Li,Yan Yang,Xiuli Zhang,Jinren Lu,Yiming Li,Mutai Bao
Although exopolysaccharide (EPS) has been applied to various fields, EPS for UVR-mediated oxidative stress repair still needs further exploration. In this study, a novel EPS was isolated from the fermentation medium of Bacillus sp. QDR3-1 and its yield was 4.8 g/L (pH 8.0, 12 % glucose, 30 °C and 6 % NaCl). The pure fraction (named EPS-M1) was purified by DEAE-cellulose and Sephadex G-100 column. EPS-M1 was a heteropolysaccharide composed of Man, Glc, Gal, and Fuc with a molecular weight of 33.8 kDa. Scanning electron microscopy (SEM) observed a rough surface and reticular structure of EPS-M1, and EPS-M1 formed spherical aggregates in aqueous solution observed in atomic force microscopy (AFM). Thermal analysis revealed that the degradation temperature of EPS-M1 was 306 °C. Moreover, methylation and NMR analysis determined that EPS-M1 was consisted of →3)-Man p -(1→, →2,6)-Man p -(1→, →4,6)-Glc p -(1→, →3)-Glc p -(1→, →4)-Gal p -(1→, →4)-Fuc p -(1→, and T-Man p -(1→. Furthermore, the cytotoxicity and the repair ability of UVR-mediated cell damage of EPS-M1 were studied with L929 cells. The results showed that EPS-M1 had good biocompatibility and it could mitigate UVR-mediated cell damage by regulating the levels of cellular reactive oxygen species (ROS), depolarization of mitochondrial membrane potential (MMP) and Caspase-3/7 activity. Overall, the structure analysis and the protective effects of EPS against L929 cells exposed to UVR provided an experimental basis for EPS in practical applications.
研究领域:
微生物学
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生物化学
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药学
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DOI
PubMed
高影响因子
Scientific Reports
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2016
·Vol. 6
·期 1
·1-13
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IF 5.228
Raza Waseem,Ling Ning,Yang Liudong,Huang Qiwei,Shen Qirong
It is important to study the response of plant pathogens to the antibiosis traits of biocontrol microbes to design the efficient biocontrol strategies. In this study, we evaluated the role of volatile organic compounds (VOCs) produced by a biocontrol strain Bacillus amyloliquefaciens SQR-9 on the growth and virulence traits of tomato wilt pathogen Ralstonia solanacearum (RS). The VOCs of SQR-9 significantly inhibited the growth of RS on agar medium and in soil. In addition, the VOCs significantly inhibited the motility traits, production of antioxidant enzymes and exopolysaccharides, biofilm formation and tomato root colonization by RS. The strain SQR-9 produced 22 VOCs, but only nine VOCs showed 1–11% antibacterial activity against RS in their corresponding amounts; however, the consortium of all VOCs showed 70% growth inhibition of RS. The proteomics analysis showed that the VOCs of SQR-9 downregulated RS proteins related to the antioxidant activity, virulence, carbohydrate and amino acid metabolism, protein folding and translation, while the proteins involved in the ABC transporter system, amino acid synthesis, detoxification of aldehydes and ketones, methylation, protein translation and folding, and energy transfer were upregulated. This study describes the significance and effectiveness of VOCs produced by a biocontrol strain against tomato wilt pathogen.
研究领域:
植物病理学
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微生物学
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农业科学
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Xiaohan Wei,Jie Hao,Kexin Xiong,Hui Guo,Siyu Xue,Yiwei Dai,Yujiao Zhang,Yingxi Chen,Sufang Zhang
Kiwifruit, a climacteric-fruit susceptible to rapid deterioration, attains considerable value upon transformation into kiwifruit wine, thereby increasing its added value. However, a noteworthy challenge arises from its high pectin content, which negatively affects byproduct quality, necessitating pectinase treatment during processing. Commercial pectinases exhibit heightened pectin esterase activity, giving rise to safety apprehensions due to augmented methanol content during kiwifruit pectin hydrolysis. In this study, Bacillus velezensis W17-6 was screened for its high pectinase activity coupled with low pectin esterase activity. The resultant crude pectinase named PBV was acquired through fed-batch-fermentation and partial purification. Subsequently, PBV was deployed in the fermentation of Xuxiang kiwifruit wine, and its impact on overall quality was assessed through diverse analyses. The results indicated that Xuxiang kiwifruit wine with PBV treatment outperformed counterparts treated with commercial pectinases. PBV treatment not only yielded superior clarity (83.75% ± 0.47%) and juice yield (81.60% ± 0.47%) but also increased the total flavonoids content in Xuxiang kiwifruit wine by 1.2 times compared to commercial pectinase. Notably, the methanol content of PBV-treated Xuxiang kiwifruit wine was significantly lower at 36.13 ± 1.83 mg/L than that of wines treated with two commercial pectinases. PBV-treated kiwifruit wine also exhibited elevated concentrations of volatile compounds such as phenethyl alcohol, ethyl undecanoate, lauric aldehyde, isobutyric acid, and myrcene. Impressively, PBV-treated kiwifruit wine surpassed other wines in both electronic and artificial sensory analyses. In summary, PBV exhibits substantial potential in the production of low-methanol, high-quality kiwifruit wine, enhancing the flavor and nutritional value while minimizing methanol production and improving the safety profile.
研究领域:
食品科学
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酿酒学
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生物技术
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微生物学
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DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
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2023
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IF 7
Jianheng Guo,Yue Xu,Sunyan Liang,Zhengkun Zhou,Chunmei Zhang,Kui Li,Xue Peng,Sheng Qin,Ke Xing
Botrytis cinerea is a necrotrophic fungal pathogen that causes gray mold disease in more than 1400 plant species. The biocontrol effect of microbial volatile compounds on postharvest diseases has become a research hotspot. This study revealed the biocontrol effects of volatiles released by Bacillus tequilensis XK29 on B. cinerea in vitro and in vivo . The results showed that the strain XK29 volatiles inhibited the mycelial growth of B. cinerea . The inhibition effect of strain XK29 volatiles against B. cinerea was increased by optimizing the wheat seeds weight, suspension concentration and inoculation volume of strain XK29, and the pre-culture time. The volatiles of strain XK29 affected the mycelial development, the formation of conidia , and the metabolic activity of B. cinerea . Selected individual monomers and artificial mixtures showed strong antifungal activity against B. cinerea. The volatiles of strain XK29 reduced the lesion diameter and disease severity of cherry tomatoes after inoculation with B. cinerea. These results indicate that the fumigation with B. tequilensis XK29 volatiles is a promising approach to protect cherry tomatoes from gray mold disease. This is also the first study to prove the antifungal effect of B. tequilensis on B. cinerea and its biocontrol effect on postharvest cherry tomatoes.
研究领域:
植物病理学
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微生物学
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食品科学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
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2023
·
IF 7.7
Yuan Chen,Yuancai Chen,Yexia Qin
In the present study, we found that nanoparticles (Pd-NPs) were biosynthesized by Bacillus wiedmannii MSM ( B. wiedmannii MSM) with endogenous carbon under O 2 condition, and more favorablity under N 2 purging condition. XRD results indicated that crystal planes of Pd-NPs were attributed to Pd 0 (111), Pd 0 (200), Pd 0 (220), and Pd 0 (311), respectively. TEM and STEM-EDX results showed that the bio-reduction of Pd (II) ( k pd,1 =4.799 min −1 ) occurred predominately in periplasm space. FTIR results showed that the -COOH, -OH, and -NH 2 were the main groups participating in the removal of Pd (II). The aerobic reduction of Pd (II) by B. wiedmannii MSM was proposed, including extracellular superoxide radicals produced by NAD(P)H oxidases , endogenous electron donors, and O 2 -insensitive hydrogenase. These studies provide new methods and insights for reduction of Pd (II).
研究领域:
环境科学
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材料科学
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微生物学
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化学
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DOI
高影响因子
FOOD CONTROL
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2023
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IF 6
Suping Li,Jingyu Hu,Shiqi Ning,Wei Li,Ru Jiang,Jianguo Huang,Yong Li
Citrus fruit post-harvest decay has caused economic loss for fruit-dependent industries worldwide in recent decades. However, bio-preservatives offer a promising and sustainable solution. In this study, culture experiments were carried out to assess the ability of Bacillus velezensis HY19, which we previously identified, to inhibit common pathogenic fungi in postharvest citrus fruit. HY19 was capable of producing extracellular siderophores and hydrolases (protease, phosphatase, and cellulase). The cells and metabolites (fermentation broth and volatiles) of the bacterium exhibited strong suppression against Penicillium italicum , P. digitatum , and Colletotrichum gloeosporioides . LC-MS analysis of B. velezensis HY19 fermentation broth (BFB) revealed 13 antimicrobial substances (simvastatin, cinnamic acid, cyclopentanone, etc.), 3 antifungal pesticide intermediates (M-cresol, 4-hydroxybenzaldehyde, and L-menthyl acetoacetate), and P-salicylic acid. GC-MS detected 18 antimicrobial substances (2-nonanol, 2-nonanone, benzoic acid, etc.) in HY19's volatile organic compounds (VOCs). Transcriptome analysis of P. digitatum exposed to BFB stress revealed that numerous genes involved in diverse biological processes, such as DNA replication and cell wall biosynthesis, were differentially expressed. Moreover, BFB inhibited the expression of multiple genes responsible for the biosynthesis of N-glycan and proteins in P. digitatum . BFB enhanced antioxidant enzyme activities in citrus peels, reduced electrolyte leakage and reactive oxygen species (ROS) accumulation, and induced the disease resistance in citrus fruit, leading to the decrease in the infection of pathogenic fungi on citrus fruit. During storage at low-temperature (4–8 °C), BFB inhibited the disease incidence in citrus fruit for up to 90 days and achieved over 60% control efficacy. Additionally, BFB had no impact on fruit quality, making it a lower risk option. Considering the benefits of sustainability and environmentally friendly options, it could be concluded that BFB was an effective alternative for controlling citrus diseases and had the potential to be used as a fruit preservative.
研究领域:
植物病理学
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食品科学
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微生物学
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农业生物技术
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2023
·Vol. 30
·期 31
·76867-76880
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IF 5.8
Huang Jiaqing,Liu Cenwei,Price Gordon W.,Wang Yixiang
Bacillus cellulasensis Zn-B isolated from vegetable soil was highly adaptable to Zinc (Zn) and Cadmium (Cd). Cd, but not Zn, adversely affected the total protein spectrum and functional groups of Bacillus cellulasensis Zn-B. Up to 31 metabolic pathways and 216 metabolites of Bacillus cellulasensis Zn-B were significantly changed by Zn and Cd (Zn&Cd). Some metabolic pathways and metabolites related to functional groups of sulfhydryl (-SH) and amine (-NH-) metabolism were enhanced by Zn&Cd addition. The cellulase activity of Bacillus cellulasensis Zn-B was up to 8.58 U mL −1 , increased to 10.77 U mL −1 in Bacillus cellulasensis Zn-B + 300 mg L −1 Zn, and maintained at 6.13 U mL −1 in Bacillus cellulasensis Zn-B + 50 mg L −1 Cd. The vegetables’ cellulose content was decreased by 25.05–52.37% and 40.28–70.70% under the action of Bacillus cellulasensis Zn-B and Bacillus cellulasensis Zn-B + 300 mg L −1 Zn. Those results demonstrated that Zn could significantly enhance cellulase activity and biodegradability of Bacillus cellulasensis Zn-B to vegetable cellulose. Bacillus cellulasensis Zn-B can survive in vegetable soil accumulated with Zn&Cd. The tolerance concentration and adsorption capacity of Bacillus cellulasensis Zn-B to Zn were up to 300 mg L −1 and 56.85%, indicating that Bacillus cellulasensis Zn-B acting as a thermostability biological agent had an essential advantage in accelerating the degradation of discarded vegetables by Zn and were beneficial to maintain organic matter content of vegetable soil. Graphical Abstract
研究领域:
微生物学
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环境科学
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土壤学
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生物化学
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Si Cheng,Chao Yin,Keying Li,Zhe Liu,Qingyan Pan,Xifeng Zuo,Ailing Guo,Huawei Ma
Films with excellent mechanical properties and antimicrobial activity have potential applications in food preservation. The antimicrobial film was prepared by blending probiotic Bacillus velezensis 906 metabolites (906), potassium sorbate (PS), and polyvinyl alcohol (PVA). The film was characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermal stability analysis. The mechanical, barrier, and antimicrobial properties of the film were tested. The results of the physicochemical analysis indicated that, compared to the pure PVA film, the film's mechanical properties decreased, while its barrier properties and hydrophobicity increased with the addition of PS and 906, respectively. The results of the antimicrobial tests revealed that the blend film exhibited significant antimicrobial activity against Vibrio parahaemolyticus, Psychrobacter maritimus , Oceanobacillus kimchii, Pseudomonas aeruginosa , and Listeria monocytogenes . SEM demonstrated good fusion between the components of the PVA/PS/906 film, resulting in a dense and crack-free structure. FTIR suggested that PS and 906 enhance molecular interactions within the film matrix. XRD revealed that the film was crystallized, with 906 increasing the crystallinity of the bonds between the film components. Thermal analysis indicated the thermal stability of the films was enhanced. Consequently, the PVA/PS/906 blend film shows significant potential for application in the preservation of aquatic products.
研究领域:
材料科学
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食品科学
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微生物学
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化学
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DOI
高影响因子
BIOTECHNOLOGY LETTERS
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2019
·Vol. 41
·期 10
·1223-1232
·
IF 2.154
Zhang Yin-Jun,Chen Chang-Sheng,Liu Hao-Tian,Chen Jia-Lin,Xia Ying,Wu Shi-Jin
Objective To purify an esterase which can selectively hydrolyze ( R,S )-ethyl indoline-2-carboxylate to produce ( S )-indoline-2-carboxylic acid and characterize its enzymatic properties. Results An intracellular esterase from Bacillus aryabhattai B8W22 was isolated and the purified protein was identified as a carboxylesterase by MALDI-TOF mass spectrometry. The enzyme (named BaCE) was 59.03-fold purification determined to be of approximately 35 kDa. Its specific activity was 0.574 U/mL with 20% yield. The enzyme showed maximum activity at pH 8.5 and 30 °C and was stable at 20–30 °C using p NPB as the substrate. The K m , V max , k cat and k cat /K m of the esterase were 0.52 mM, 6.39 μM/min, 26.87 min −1 and 51.67 mM/min, respectively. The esterase demonstrated high enantioselectivity toward ( S )-ethyl indoline-2-carboxylate with 96.55% e.e. p at 44.39% conversion, corresponding to an E value of 133.45. Conclusions In this study, a new esterase BaCE with an apparent molecular mass of 35 kDa was purified to homogeneity for the first time. The esterase from Bacillus aryabhattai B8W22 was isolated with a purification more than 59-fold and a yield of 20% by anion exchange chromatography and hydrophobic interaction chromatography. And its biochemical characterization were described in detail with p NPB as substrate. It displayed high enantioselectivity toward ( S )-ethyl indoline-2-carboxylate. We next plan to highly express esterase BaCE in Escherichia coli , and apply it to industrial production of ( S )-indoline-2-carboxylic acid.
研究领域:
生物化学
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酶学
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微生物学
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DOI
PubMed
高影响因子
Engineering
·
2025
·
IF 11.6
Wenyue Yan,Baogang Zhang,Yi’na Li,Jianping Lu,Yangmei Fei,Shungui Zhou,Hailiang Dong,Fang Huang
Microbial vanadate (V(V)) reduction is a key process for environmental geochemistry and detoxification of vanadium (V). However, the electron transfer pathways and V isotope fractionation involved in this process are not yet fully understood. In this study, the V(V) reduction mechanisms with concomitant V isotope fractionation by the gram-positive bacterium Bacillus subtilis ( B. subtilis ) and the gram-negative bacterium Thauera humireducens ( T. humireducens ) were investigated. Both strains could effectively reduce V(V), and (90.5% ± 1.6%) and (93.0% ± 1.8%) of V(V) at an initial concentration of 50 mg·L −1 was removed over 10 d incubation, respectively. V(V) was bioreduced to insoluble vanadium (IV), which was distributed both inside and outside the cells. Electron transfer via cytochrome C, nicotinamide adenine dinucleotide, and glutathione played critical roles in V(V) reduction. Metabolomic analysis showed that differentially enriched metabolites (quinone, biotin, and riboflavin) mediated electron transfer in both strains. The aqueous V in the remaining solution became isotopically heavier as V(V) bioreduction proceeded. The obtained V isotope composition dynamics followed a Rayleigh fractionation model, and the isotope enrichment factor ( ε ) was (–0.54‰ ± 0.04‰) for B. subtilis and (–0.32‰ ± 0.03‰) for T. humireducens , with an insignificant difference. This study provides molecular insights into electron transfer for V(V) bioreduction and reveals V isotope fractionation during this bioprocess, which is helpful for understanding V biogeochemistry and developing novel strategies for V remediation.
研究领域:
环境微生物学
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地球化学
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生物化学
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DOI
高影响因子
BIODEGRADATION
·
2024
·
IF 3.1
Sun Shanshan,Wei Ran,Hu Siyi,Yang Meiyu,Ni Jinzhi
Considerable efforts that isolate and characterize degrading bacteria for polycyclic aromatic hydrocarbons (PAHs) have focused on contaminated environments so far. Here we isolated three distinctive pyrene (PYR)-degrading bacteria from a paddy soil that was not contaminated with PAHs. These included a novel Bacillus sp. PyB-9 and efficient degraders, Shigella sp. PyB-6 and Agromyces sp. PyB-10. All three strains could utilize naphthalene, phenanthrene, anthracene, fluoranthene and PYR as sole carbon sources, and degraded PYR in a range of temperatures (27–37 °C) and pH (5–8). Strains PyB-6 and PyB-10 almost completely degraded 50 mg L −1 PYR within 15 days, and 75.5% and 98.9% of 100 mg L −1 PYR in 27 days, respectively. The kinetics of PYR biodegradation was well represented by the Gompertz model. Ten and twelve PYR metabolites were identified in PYR degradation process by strains PyB-6 and PyB-10, respectively. Chemical analyses demonstrated that the degradation mechanisms of PYR were the same for strains PyB-6 and PyB-10 with initial dioxygenation mainly on C-4,5 positions of PYR. The degradation of 4,5-phenanthrenedicarboxylic acid was branched to 4-phenanthrenecarboxylic acid pathway and 5-hydroxy-4-phenanthrenecarboxylic acid pathway, both of which played important roles in PYR degradation by strains PyB-6 and PyB-10. To our knowledge, Shigella sp. and Agromyces sp. were found for the first time to possess the capability for PAHs degradation. These findings contributed to upgrading the bank of microbial resource and knowledge on PAH biodegradation.
研究领域:
环境科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2022
·
IF 5.911
Na Li,Xiaoming Yuan,Chun Li,Nuo Chen,Juan Wang,Bo Chen,Shubo Yu,Pengfei Yu,Junhui Zhang,Haiyan Zeng,Shi Wu,Xiaojuan Yang,Meiyan Yang,Jumei Zhang,Qingping Wu,Yu Ding
Bacillus cereus is a common foodborne pathogen that causes vomiting and diarrheal symptoms. Due to its spore-forming ability, B. cereus can resist physical sterilization and possess a relatively high contamination level in dairy products; therefore, it is necessary to develop an efficient strategy to control the growth of B. cereus . In this study, a novel bacteriophage, named DLn1, was isolated and characterized, and its endolysin was expressed. Morphological and genomic analyses revealed that the phage is a new species belonging to the Northropvirinae subfamily of the Salasmaviridae family. The life cycle and stability assays showed that the phage DLn1 exhibited a short latent period (15 min) and high burst size (618 plaque-forming units (PFU)/cell) and was tolerant to a wide range of pH (4–10) and temperature (4–55 °C) conditions. This lytic phage had narrow but specific host range to B. cereus strains, and could effectively reduce the number of B. cereus in milk within 6 h. More interestingly, the purified endolysin of phage DLn1 had a much wider lytic range and the inhibitory effect against B. cereus in milk was more efficient. Taken together, the new phage DLn1 and its endolysin could be promising biocontrol agents against B. cereus in dairy products.
研究领域:
微生物学
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食品安全
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生物技术
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）