JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2020
·
IF 3.004
Dian-Bing Zhou,Fang Han,Lei Ding,Wei Song,Ya-Ning Lv,Yan-Yun Hu,Yu-Xin Liu,Xuan Sheng,Ping Zheng
C 60 -based magnetic nanospheres were synthesized by coating Fe 3 O 4 nanospheres with silica, then modifying with 3-aminopropyltriethoxysilane as a linker and a C 60 fullerene stationary phase. The morphologies, magnetic properties, infrared absorption and carbon content of magnetic nanospheres were studied by TEM, VSM, FTIR and carbon and sulfur analyzer. The magnetic nanospheres were employed for the magnetic solid-phase extraction (MSPE) of 16 polycyclic aromatic hydrocarbons (PAHs) in nine Chinese herbal medicines. The analyses were conducted by isotope dilution gas chromatography–mass spectrometry. The main parameters influencing the extraction, including extraction solvent, adsorbent amount, and extraction time were optimized. Method validation showed that the limit of detection (LOD) was 0.02–0.11 µg/kg, and the limit of quantification (LOQ) was 0.07–0.36 µg/kg. The spiked recoveries rates for 16 PAHs in white peony root were 84.7–107.2%. The relative standard deviation (RSD) was 1.7–8.4%. The established method was further used for the determination 16 PAHs in nine Chinese herbal medicines. Total content of 16 PAHs varied from 73.6 µg/kg (fructus lycii) to 2172.6 µg/kg (astragalus root). The results indicate that the pollution of PAHs in Chinese herbal medicines is serious. The established method can effective detect PAHs contamination in Chinese herbal medicines.
研究领域:
分析化学
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环境科学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Zhe Qiao,Hongfang Zhang,Yuanzhen Zhou,Jianbin Zheng
Label-free biosensing based on the nanoporous anodic alumina (NAA) membrane emerged as a versatile biosensing platform in the recent decade. In the present work, we developed a new immunosensing strategy based on the nanochannels of NAA and the ion pair interaction mediated by electrochemistry of C60. The NAA served as the matrix for the immobilization of the capture antibodies. The incubation of target antigens resulted in the formation of the immunocomplexes and thus an increase of the steric hindrance of the nanochannels. Therefore, the concentration of the redox probe transported through the nanochannels decreases, which can be detected at the working electrode modified with C60. Herein, we initially found that the cathodic peak ascribed to the reduction of C60 to C60– was obviously enhanced by the presence of the redox probe K3[Fe(CN)6] and which was contributed to the formation of a ternary ion association complex among C60, tetraoctylammonium bromide, and K3[Fe(CN)6]. Therefore, the transportation of K3[Fe(CN)6] though the NAA-based bionanochannels can be detected by a C60 modified electrode with an amplified signal. Choosing human epididymis protein 4 (HE4) as the model target, a linear range of 1.0 ng mL–1 to 100 ng mL–1 can be established between the peak current obtained from the differential pulse voltammetric response of the platform and the concentration of HE4. The detection limit was 0.2 ng mL–1. This study not only provides a new avenue to develop the other nanochannel-based biosensing platform for a variety of other disease biomarkers but also contributes to the electrochemistry of fullerene.
研究领域:
生物医学工程
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电化学传感
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免疫学
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纳米医学
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分子诊断
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转化医学
✓ 已验证
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 17
·4073
·
IF 4.2
Beihua Xu,Zhenyu Wang,Huimin Zhang,Xiao Xu,Mengjie Tang,Gang Wang,Zhongpeng Ding,Ruihao Yu,Meihong Ding,Ting Zhang,Senlin Shi
Objective: The aim of this study was to optimize the formulation of a C60-modified self-microemulsifying drug delivery system loaded with triptolide (C60-SMEDDS/TP) and evaluate the cytoprotective effect of the C60-SMEDDS/TP on normal human cells. Results: The C60-SMEDDS/TP exhibited rapid emulsification, an optimal particle size distribution of 50 ± 0.19 nm (PDI 0.211 ± 0.049), and a near-neutral zeta potential of −1.60 mV. The release kinetics of TP from the C60-SMEDDS/TP exhibited a sustained release profile and followed pseudo-first-order release kinetics. Cellular proliferation and apoptosis analysis indicated that the C60-SMEDDS/TP (with a mass ratio of TP: DSPE-PEG-C60 = 1:10) exhibited lower toxicity towards L02 and GES-1 cells. This was demonstrated by a higher IC50 (40.88 nM on L02 cells and 17.22 nM on GES-1 cells) compared to free TP (21.3 nM and 11.1 nM), and a lower apoptosis rate (20.8% on L02 cells and 26.3% on GES-1 cells, respectively) compared to free TP (50.5% and 47.0%) at a concentration of 50 nM. In comparison to the free TP group, L02 cells and GES-1 cells exposed to the C60-SMEDDS/TP exhibited a significant decrease in intracellular ROS and an increase in mitochondrial membrane potential (ΔψM). On the other hand, the C60-SMEDDS/TP demonstrated a similar inhibitory effect on BEL-7402 cells (IC50 = 28.9 nM) and HepG2 cells (IC50 = 107.6 nM), comparable to that of the free TP (27.2 nM and 90.4 nM). The C60-SMEDDS/TP group also exhibited a similar intracellular level of ROS and mitochondrial membrane potential compared to the SMEDDS/TP and free TP groups. Method: Fullerenol-Grafted Distearoyl Phosphatidylethanolamine-Polyethylene Glycol (DSPE-PEG-C60) was synthesized and applied in the self-microemulsifying drug delivery system. The C60-SMEDDS/TP was formulated using Cremophor EL, medium-chain triglycerides (MCT), PEG-400, and DSPE-PEG-C60, and loaded with triptolide (TP). The toxicity and bioactivity of the C60-SMEDDS/TP were assessed using normal human liver cell lines (L02 cells), normal human gastric mucosal epithelial cell lines (GES-1 cells), and liver cancer cell lines (BEL-7402 cells and HepG2 cells). The production of reactive oxygen species (ROS) after the C60-SMEDDS/TP treatment was assessed using 2′,7′-dichlorofluorescein diacetate (DCFDA) staining. The alterations in mitochondrial membrane potential (ΔψM) were assessed by measuring JC-1 fluorescence. Conclusions: The cytoprotection provided by the C60-SMEDDS/TP favored normal cells (L02 and GES-1) over tumor cells (BEL-7402 and HepG2 cells) in vitro. This suggests a promising approach for the safe and effective treatment of TP.
研究领域:
药理学
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生物医学工程
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 16
·13217-13223
·
IF 3.289
Xiaomin Fu,Zhengcong Gu,Qiyi Lu,Jiayao Liao,Shihong Chen
C60/graphite-like carbon nitride nanosheet (C60/g-C3N4 NS) hybrids were prepared by a simple π–π interaction, and used to construct a solid state electrochemiluminescence (ECL) sensor. Based on the fact that melamine could efficiently quench the ECL intensity of g-C3N4 NS, such a sensor achieved detection of melamine. The possible quenching mechanisms were discussed. Due to the combination of the excellent ECL behavior of g-C3N4 NS with the outstanding electronic conductivity of C60, the prepared sensor exhibited a response for melamine with a wide linear range of 5.0 × 10−13 to 2.7 × 10−11 M and 2.7 × 10−11 to 1.9 × 10−8 M. The detection limit was 1.3 × 10−13 M. In this work, the linear range of melamine was improved by six orders of magnitude and the detection limit as low as sub-picomolar. Such a construction strategy provided a simple, sensitive, low-cost and green detection method for melamine.
研究领域:
分析化学
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材料科学
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食品安全检测
✓ 已验证
产品关联
DOI
高影响因子
Journal of Inorganic and Organometallic Polymers and Materials
·
2023
·Vol. 34
·期 4
·1751-1764
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IF 4
Zhou Zhuyou,Zou Congyang,Wang Yong,Liu Menghe,Ji Yufeng
A one-step solvothermal synthesis technique was employed to fabricate C 60 /MIL-101(Fe) composites. The C 60 /MIL-101(Fe) composites showed a better performance in the degradation of methylene blue (MB) and tetracycline hydrochloride (TCH) when compared to MIL-101(Fe) in the photo-Fenton system, which can be credited to the charge transfer and separation facilitated by the addition of C 60 . Results showed that C 60 /MIL-101(Fe) had the capacity to nearly completely eliminate TCH and MB over a broad pH range. The catalysts demonstrated remarkable catalytic degradation on various organic pollutants and water samples. Through active substance quenching experiments and EPR, it was determined that hydroxyl radical (·OH) is the main factor in the photocatalytic reaction process.
研究领域:
环境科学
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材料科学
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化学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Qing Zhou,Guanghui Li,Kaiyang Chen,Hong Yang,Mengran Yang,Yuye Zhang,Yakun Wan,Yanfei Shen,Yuanjian Zhang
Due to exceptional electron-accepting ability, light-absorption, and a delocalized conjugated structure, buckminsterfullerene (C60) has attracted fascinating interest in the field of organic solar cells. However, poor delocalization and accumulation of electrons for pristine C60 in physiological aqueous solution and difficulties in conjugation with biomolecules limit its extended photovoltaic applications in bioassay. Herein, we reported the noncovalent coupling of C60 to an electronically complementary porphyrin-derived metal–organic framework (PCN-224) with carboxyl-group terminals. Such assembly not only offered a friendly interface for bioconjugation but also resulted in a long-range ordering C60@PCN-224 donor–acceptor system that demonstrated an unprecedented photocurrent enhancement up to 10 times with respect to each component. As an example, by further cooperating with Nanobodies, the as-prepared C60@PCN-224 was applied to a photoelectrochemical (PEC) immunosensor for S100 calcium-binding protein B with by far the most promising detection activities. This work may open a new venue to unlock the great potential of C60 in PEC biosensing with excellent performances.
研究领域:
材料科学
·
生物化学
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分析化学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2022
·Vol. 24
·期 39
·24495-24502
·
IF 3.945
Min Zhu,Jiale Ding,Sen Niu,Yunhe Zhang,Guibin Wang
The construction of molecules with effective photoinduced intramolecular electron transfer (PET) and energy transfer (ET) processes is critical for the enhancement of the nonlinear optical (NLO) properties. A novel D–π–A type compound, namely tetra (C60)–LaPc, has been constructed from a molecular design perspective, wherein tetra-formyl phthalocyanine is used as a donor, four C60 as acceptors and the phenylacetylene group is introduced into it as π-electron bridge, which can increase the molecular conjugation, reduce the spatial site resistance and expand the coplanarity of the molecule, thus making the intramolecular charge transfer easier. Tetra (C60)–LaPc achieves excellent NLO properties with a giant nonlinear absorption coefficient (45 cm GW−1 and large third-order susceptibility (4.05 × 10−10 esu), which are superior to those of LaPc and C60. In addition to exhibiting a superior nonlinear optical response at 532 nm compared to the single component, tetra (C60)–LaPc also broadens the limiting range to the near-infrared region, showing a significant enhancement of the optical nonlinearity at 1064 nm. This can be attributed to the synergistic effect of the different non–linear absorption mechanisms between C60 and LaPc and the efficient PET process.
研究领域:
材料科学
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光学
·
化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2020
·
IF 8.247
JingYan Liu,Yan Guo,MengHua Zhu,YuanChao Li,Xin Li
In inverted planar perovskite solar cells (PSCs), fullerene (C 60 ) and its derivatives are widely utilized as electron-transport layer (ETL) materials, however, possess some drawbacks such as low electron mobility , interfacial degradation, and relatively inferior stability. In this work, we develop a facile thermal annealing-free hydrothermal route to synthesize high-crystalline SnO 2 nanocrystals through controlling the crystallization process with HClO 4 . An interlayer of SnO 2 nanocrystals is inserted between C 60 ETL and Ag electrode. The SnO 2 interlayer can not only serve as an ETL to help improve electron extraction and transport but also act as a passivation/barrier layer to prevent oxygen and moisture ingress and ion migration. Based on the p-i-n structure of fluorine-doped tin oxide (FTO)/NiO x /MAPbI 3 /C 60 /SnO 2 /Ag, the power conversion efficiency (PCE) of the champion device is up to 16.36% with almost negligible hysteresis . The unencapsulated inverted PSCs retain more than 80% of its initial PCE value after storage in atmosphere for 90 days (>2100 h), and degrade only about 20% its initial efficiency after 41 h under heating at 85 °C. More important, all the preparation processes are performed under ambient conditions. Our work provides a viable strategy for assembling highly efficient and stable inverted PSCs in the air.
研究领域:
材料科学
·
太阳能
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纳米技术
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电化学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Nano Materials
·
2020
·
IF 0
Jia-Hui Yan,Yi-Shun Ji,Man-Li Yang,Jun Fu,Hu Shan,Li−Li Wang,Lei Zhang,Jing-Yuan Xiao,Xiao-Ying Kong,Jin-Sheng Shi
Photodynamic therapy (PDT), a promising noninvasive tumor treatment strategy, is limited by poor biocompatibility, high cost, and complicated synthesis procedures of rare-earth up-conversion materials. Herein, natural melanin particles (MNPs), excellent photothermal therapy (PTT) materials, were found to significantly promote production of reactive oxygen species (ROS) under 808 nm irradiation when cross-linked with C60, whereas synthetic polydopamine had no such property. On the basis of this special character of MNP, MNP-C60-hyaluronic acid (MC60H) nanoparticles, independent of rare-earth up-conversion materials, were prepared for multimodal attack toward a tumor under 808 nm irradiation. After targeting recognition by hyaluronic acid (HA), MC60H could not only efficiently promote temperature rise and the production ROS in tumor but also activate the prominent immunotherapy and help tumor immunosuppressed M2-type macrophages transformed into antitumor M1-type. Under the premise of high biosecurity and simple preparing strategy, the versatile nanoparticle material, MC60H, is utilized for collaborative PTT, PDT, and immunotherapy under NIR excitation without participation of any rare-earth elements. The character of promoting ROS production of C60 by MNP, which is a natural biomaterial, is first reported in this paper, which provides a good demonstration of MC60H in application of a synergistic antitumor strategy without the dependence of traditional up-conversion material.
研究领域:
生物医学工程
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纳米技术
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癌症治疗
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材料科学
✓ 已验证
产品关联
DOI
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2021
·Vol. 35
·期 19
·e9169
·
IF 2.419
Zhang-Na Qin,Jun Ding,Qiong-Wei Yu,Ping Zhou,Yu-Qi Feng
Rationale 3-Monochloropropane-1,2-diol (3-MCPD) is a well-known contaminant formed in food thermal processing, which could be found in a variety of foodstuffs. Due to its potential carcinogenicity, it was essential to quickly develop a rapid and high-throughput analytical method to monitor 3-MCPD in foodstuffs, which is described in this study. Methods 3-MCPD was extracted from foodstuffs and then was derivatized with a boronic acid–modified C 60 (B-C 60 ) through the boronic acid-diol reaction. Microwave heating was used to accelerate the derivatization reaction. Mass spectrometry (MS) analysis was conducted using matrix-assisted laser desorption/ionization-MS (MALDI-MS). The application of the method was validated using various smoked food samples. Results The chemical derivatization of 3-MCPD with B-C 60 enabled the addition of a C 60 -tag to 3-MCPD. High-throughput analysis of the sample within 0.5 h was realized. A good linear range from 0.02 to 1.5 μg mL −1 for 3-MCPD was obtained, with a detection limit of 0.005 μg mL −1 . The recoveries in spiked foodstuffs ranged from 85.4% to 115.1% with relative standard deviations of 2.0%–14.2%. This method was successfully applied to detect 3-MCPD in smoked foodstuffs. Conclusions A quantitative method was developed for the detection of 3-MCPD in foodstuffs using B-C 60 derivatization combined with MALDI-MS strategy. This proposed method may serve as a potential platform for the rapid and high-throughput analysis of 3-MCPD in foodstuffs for the purpose of food safety control.
研究领域:
食品科学
·
分析化学
·
质谱分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Zhang-Na Qin,Jun Ding,Qiong-Wei Yu,Chu-Bo Qi,Dong-Mei Wu,Ping Zhou,Yu-Qi Feng
Perturbation of thiol homeostasis in biological fluids are thought to be associated with several diseases, and reliable analytical methods for the determination of low molecular weight (LMW) thiols in human plasma or serum are thus required. In this study, a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method is described for high throughput determination of four LMW thiols (glutathione, cysteine, homocysteine and cysteinylglycine) in human serum. It is based on the use of a bromoacetyl functionalized C60 (Br–C60) as a derivatization reagent to label thiols. The Br–C60 labeling can add an 832-Da tag to thiols, which moves thiol signals to high mass region and effectively avoids the signal interference generated by the traditional MALDI matrix below 800 Da. The labeling can be completed within 5 min under microwave-assisted condition. Thereby, the Br–C60 labeling based MALDI-TOF MS analytical method can achieve high throughput analysis of LMW thiols in serum. Good linearities of the method for the thiols in human serum were obtained in the range of 0.5–500.0 μM with correlation coefficient (R) greater than 0.9960. The limit of detection is in the range of 0.07–0.18 μM for the investigated thiols in human serum with relative standard deviations of lower than 13.5% and recoveries ranging from 81.9 to 117.1%. Using the method, four thiols in microliter serum samples of breast cancer (BC) patients were determined. The result showed that the contents of the four thiols in BC serum samples significantly changed compared to the healthy control (HC).
研究领域:
分析化学
·
医学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 6
·4653-4660
·
IF 3.201
Peipei Chen,Ting Wang,Xiangli Zheng,Dong Tian,Fangquan Xia,Changli Zhou
A convenient, highly efficient and ultrasensitive electrochemical immunosensor was developed using in situ deposited silver nanoparticles (Ag NPs) as tracing tags for fetoprotein (AFP) detection. The function of the immunosensor was achieved by a co-catalytic effect on the surface of nanocomposites containing polyamidoamine (PAMAM) dendrimers coated with C60 (PAMAM-C60 NPs) and gold nanoparticles (Au NPs). On the surface of Au@PAMAM-C60 NPs labeled with an antibody (Ab2), via a sandwich-type immunoreaction, silver ions in solution were reduced to metallic Ag NPs. The electrochemical signal of the in situ deposited Ag NPs could be utilized to monitor the immunoreaction. Under optimum conditions, the peak current of the Ag NPs increased with increasing AFP concentration in the range from 0.1 pg mL−1 to 10 ng mL−1, with a detection limit of 0.03 pg mL−1. Importantly, the designed electrochemical immunoassay strategy exhibited excellent sensitivity, stability, selectivity, and reproducibility.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）