ANALYST
·
2023
·Vol. 148
·期 14
·3295-3305
·
IF 4.2
Yong Huang,Zhengqing Liu,Xiaogang Qin,Jia Liu,Yan Yang,Wei Wei
Highly sensitive protein biomarker detection is critical for the diagnosis of gastric cancer (GC), however the accurate and sensitive detection of low-abundance proteins in early-stage GC is still a challenge. Herein, a surface-enhanced Raman scattering frequency shift assay was performed on a developed microfluidic chip for the detection of GC protein biomarkers carcinoembryonic antigen (CEA) and vascular endothelial growth factor (VEGF). The chip is made up of three groups of parallel channels and each parallel channel consists of two reaction regions, enabling the simultaneous analysis of multiple biomarkers in multiple samples. The presence of CEA and VEGF in the sample can be captured by the 4-mercaptobenzoic acid (4-MBA)-conjugated antibody functionalized gold nano-sheet (GNS-) substrate, resulting in the Raman frequency shift. As a result, a typical Raman frequency shift of 4-MBA presented a linear relationship with the concentration of CEA and VEGF. The limit of detection (LOD) of the proposed SERS microfluidic chip reaches as low as 0.38 pg mL−1 for CEA and 0.82 pg mL−1 for VEGF. During the detection process, only one step of sample addition is involved, which eliminates the multiple reaction step-induced nonspecific adsorption and significantly increases the convenience and specificity. In addition, serum samples from GC patients and healthy subjects were tested and the results were in good agreement with the current gold-standard method ELISA, suggesting the potential application of the SERS microfluidic chip in clinical settings for early diagnosis and prognosis of GC.
研究领域:
医学
·
分析化学
·
纳米技术
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DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Yanan Lou,Tong Jiang,Jiangle Yi,Xiang Ren,Xueying Wang,Dawei Fan,Hongmin Ma,Qin Wei
Nanostructured materials with elegant properties have promising potentials in chemiluminescence (CL) immunoassay . However, the robust preparation of such nanomaterials is still challenging. In this work, biocompatible and easily prepared gelatin/horseradish peroxidase (Gel/HRP) nanogels were exploited as signal labels for chemiluminescence immunoassays. The catalytic property of HRP was well retained and the stability was improved after the incorporation into nanogels. The prepared protein nanogels show enhanced and glow-type luminescence behaviors in luminol-H 2 O 2 CL system. Persistent and sensitive CL immunoassay for CEA were constructed using Gel/HRP nanogels as signal labels. A wide linear response range from 0.01 to 100 ng/mL was obtained with the limit of detection was 3.39 pg/mL (S/N = 3).
研究领域:
生物材料学
·
分析化学
·
医学检测
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高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Sijing He,Qiyou Wang,Yanyan Yu,Qiujia Shi,Lin Zhang,Zuanguang Chen
A novel, label-free and inherent electroactive redox immunosensor for ultrasensitive detection of carcinoembryonic antigen (CEA) was proposed based on gold nanoparticles (AuNPs) and potassium ferricyanide-doped polyaniline (FC-PANI) nanoparticles . FC-PANI composite was synthesized via oxidative polymerization of aniline, using potassium ferricyanide (K 3 [Fe(CN) 6 ]) as both oxidant and dopant . FC-PANI acting as the signal indicator was first fixed on a gold electrode (GE) to be the signal layer. Subsequently, the negatively charged AuNPs could be adsorbed on the positively charged FC-PANI modified GE surface by electrostatic adsorption, and then to immobilize CEA antibody (anti-CEA) for the assay of CEA. The CEA concentration was measured through the decrease of amperometric signals in the corresponding specific binding of antigen and antibody. The wide linear range of the immunosensor was from 1.0 pg mL −1 to 500.0 ng mL −1 with a low detection limit of 0.1 pg mL −1 (S/N=3). The proposed method would have a potential application in clinical immunoassays with the properties of facile procedure, stability, high sensitivity and selectivity .
研究领域:
分析化学
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生物化学
·
纳米技术
·
生物传感器
·
医学诊断
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Yuxiao Yang,Min Jiang,Kaihang Cao,Mei Wu,Chulei Zhao,Hongling Li,Chenglin Hong
An electrochemical immunosensor for quantitation of carcinoembryonic antigen (CEA) based on Au-Ag/ [email protected] nanocomposites for dual signal amplification strategy was designed. Polydopamine-coated Ag/graphene hybrids were synthesized by one-step reduction and functionalization, and the dopamine was introduced to replace some reducing agents that are harmful to organisms. Silver nanoparticles modified on the electrodes produced sensitive electrochemical redox peaks. When different amounts of protein (antigen, antibody, bovine serum protein, etc.) were adsorbed on the surface of nano-silver, the surface of nano-silver was covered. The electrochemical redox peak of silver particles decreased due to the change of the effective area of bare silver particles which could react with electrodes. In addition, CEA-antibody (anti-CEA) was immobilized into a biocompatible nanocomposite of Au nanoparticles as a conductive matrix, and CEA-antibody (anti-CEA) was combined with Ag/ [email protected] to form a dual signal amplifying element. Under optimal condition, the linear relationship was obtained from 0.001 ng mL −1 to 80 ng mL −1 between current signal and CEA concentration with a detection limit of 0.286 pg mL −1 (signal-to-noise ratio is 3). The label-free electrochemical immunosensor has been successfully applied to human serum detection due to good selectivity , stability and reproducibility.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
生物医学工程
·
材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Mingzhu Fu,Lizhen Long,Heming Wang,Meixian Li,Yujiao Qiu,Jiahao Dai,Juanjuan Meng,Jun Liu
Uniform hierarchical AuNFs are in-situ fabricated on SPCE via facile one-step electrodeposition. • AuNFs provide abundant accessible binding sites for antibody immobilization and efficient electron-transfer pathways. • The developed immunosensor achieves ultra-sensitive CEA detection with an ultra-low LOD. • The as-prepared immunosensor exhibits favorable reproducibility and satisfactory long-term storage stability. This study reports a highly sensitive electrochemical immunosensor based on branched hierarchical gold nanoflowers (AuNFs) for the selective detection of carcinoembryonic antigen (CEA). Uniform AuNFs were synthesized by optimized electrochemical deposition parameters on the screen-printed carbon electrode (SPCE). The distinct hierarchical branched structure of AuNFs endows the electrode interface with abundant uniformly distributed active sites and efficient electron-transport pathways. By integrating MUA self-assembly with EDC/NHS-mediated covalent antibody immobilization, a highly specific and stable electrochemical immunosensor was successfully developed for ultrasensitive CEA detection. The proposed immunosensor achieves a wide linear detection range from 0.1 to 100,000 pg·mL −1 and a low limit of detection (LOD) of 0.069 fg·mL −1 for CEA detection. Furthermore, the immunosensor exhibits excellent selectivity, reproducibility, and long-term storage stability, enabling reliable quantification of CEA in biological samples. This study demonstrates that the AuNFs-modified SPCEs immunosensor is a promising high-performance platform for point-of-care (POCT) tumor biomarker detection, with great application potential in early clinical cancer screening and postoperative dynamic monitoring. AuNFs modified screen-printed carbon electrodes (SPCEs) possesses excellent electrical conductivity and plasticity, which facilitates the construction of CEA-specific sensing interfaces and improve detection sensitivity. Download: Download high-res image (175KB) Download: Download full-size image
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高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 4
·826-833
·
IF 4.872
Zhenzhen Lin,Shuzhen Lv,Kangyao Zhang,Dianping Tang
This work designs a simple low-cost visual fluorescence immunoassay for disease-related biomarkers (carcinoembryonic antigen, CEA, used as a model protein) in biological fluids, based on the structural and optical transformation of CdTe quantum dots (QDs) immobilized on paper induced by dissolved silver ions (Ag+) from silver nanoparticles (AgNPs) via a cation-exchange reaction. A sandwich-type immunoreaction was initially carried out in a removable polystyrene high-binding microplate coated with monoclonal anti-CEA capture antibody by using AgNP-labeled polyclonal anti-CEA antibody as the detection antibody. Thereafter, the carried AgNPs accompanying the sandwiched immunocomplexes were dissolved by acid to release numerous silver ions, which induced the ion-exchange reaction with the immobilized CdTe QDs on the paper (attached onto the microplate lid) for the Cd-to-Ag transformation, thus resulting in the quenching of the visual fluorescence from the CdTe QDs owing to Ag2Te formation. Under optimal conditions, the fluorescence intensity decreased with the increasing CEA concentration from 0.02 to 50 ng mL−1 with a detection limit of 5.6 pg mL−1. Further, a visual assay based on a CdTe QD-based paper sensor was developed for CEA detection, and 5.0 pg mL−1 CEA could be discriminated with the naked eye. In addition, our strategy displayed high specificity, good reproducibility and acceptable accuracy for analyzing human serum specimens with consistent results obtained using the commercialized enzyme-linked immunosorbent assay (ELISA) method.
研究领域:
分析化学
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生物传感器
·
生物医学工程
·
分子诊断
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DOI
PubMed
高影响因子
ChemElectroChem
·
2013
·Vol. 1
·期 2
·441-447
·
IF 0
Qunfang Li,Dianping Tang,Fangming Lou,Ximei Yang,Guonan Chen
Graphical Multitasker: A sensitive and feasible multiplexed immunoassay protocol for simultaneous electrochemical determination of biomarkers is presented. The assay uses multifunctionalized graphene nanotags on a cyclodextrin-modified immunosensor. A sensitive and feasible multiplexed immunoassay protocol for simultaneous electrochemical determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was developed using multifunctionalized graphene nanotags on a cyclodextrin-modified immunosensor. The sensor was fabricated via the guest–host chemistry between the immobilized cyclodextrin and anti-CEA/anti-AFP antibodies. Two nanotags including cyclodextrin-thionine-graphene and cyclodextrin-ferrocene-graphene nanostructures were synthesized based on a wet chemistry method. The synthesized nanotags were employed for the labelling of horseradish peroxidase (HRP)–anti-AFP and HRP–anti-CEA conjugates, respectively. With a sandwich-type assay format, the signal was monitored through the labelling of HRP toward catalytic reduction of H 2 O 2 at various peak potentials in the presence of thionine and ferrocene, respectively. Under optimal conditions, the multiplexed immunoassay enabled the simultaneous determination of CEA and AFP with wide working ranges of 0.001–60 ng mL −1 for AFP and 0.003–40 ng mL −1 for CEA. The detection limits were 0.5 pg ml −1 for AFP and 0.8 pg ml −1 for CEA (at 3 s B ). No obvious nonspecific adsorption and cross-talk were observed during a series of analyses to detect target analytes. Intra- and inter-assay coefficients of variation (CV) were below 9.5 %. Importantly, the methodology was also evaluated for the analysis of AFP and CEA in the clinical human serum specimens, receiving a good relationship between the developed immunoassay and a commercialized electrochemiluminescence enzyme-linked immunoassay.
研究领域:
生物医学工程
·
分析化学
·
材料科学
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DOI
ACS Applied Nano Materials
·
2023
·
IF 5.9
Lulu Kong,DeZhu Chen,Xiaolong Zhang,Lin Zhou,Yongming Deng,Shaohua Wei
The growth of oxides on the surface of noble metals and the introduction of alloys are effective methods to enhance the catalytic activity of noble metals. However, the fabrication of noble metal oxides always involves harsh experimental conditions and the oxidation degree could not be controlled. Herein, a simple, fast, and controlled Pd oxidation method by mixing Hg2+ and Pd nanosheets (PdNSs) under mild conditions to form PdxHgy(PdO)z (PHOs) was reported, which consists of the Pd–Hg alloy and PdO. The density functional theory (DFT) calculation and experiment results proved that the Hg atom incorporation promotes the nearby Pd–Pd bond breaking, initiating a cascade of PdO generation. More precisely, 1 Hg atom incorporation induced ∼8 Pd atom oxidation, ensuring that the oxidation degree of Pd could be easily controlled by adjusting the ratio of Hg2+ and PdNS. As a peroxidase-mimicking nanozyme, the PHO affinity for its substrate could be 21.7- and 123.6-fold greater than that of the PdNS and the natural peroxidase (HRP), respectively. For the colorimetric immunoassay of carcinoembryonic antigen (CEA) detection, PHO has an ultralow detection limit of 1.33 pg mL–1, which is ∼8.6 and ∼282 times lower than that of the PdNS and the natural HRP, respectively.
研究领域:
生物化学
·
医学诊断
·
材料科学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Yiju Song,Kaihang Cao,Wenjun Li,Chaoyun Ma,Xiuwen Qiao,Hongling Li,Chenglin Hong
A three-dimensional array of reduced graphene oxide/MoS 2 @ polyaniline-layered nanosheets (denoted as rGO/MoS 2 @ PANI) has a novel structure of intercalated MoS 2 /PANI nanosheets arrays arranged on rGO that can be used as carcinoembryonic antigen (CEA) electrochemical immunosensors (detectors). In this graded nanosheet, the PANI was embedded in the MoS 2 interlayer. The MoS 2 /PANI nanosheets themselves limit volumetric changes when arranged as MoS 2 /PANI nanosheet arrays on rGO nanosheets to provide tight and sufficient heterogeneity. PANI locates on the interface itself and contains a large amount of –NH 2 that can bind many antibodies. Simultaneously, we investigated the optimal amount of aniline to add. We found that these arrays had excellent film thickness and conductivity for improving CEA detection sensitivity. The tested immunosensor achieved sensitive and specific detection of CEA, with a detection limit of 0.3 pg mL −1 . Under the optimal conditions, the prepared immunosensor exhibited acceptable reproducibility and selectivity and excellent stability. Satisfactory results of human serum samples indicated the potential application of the immunosensor for clinical monitoring of tumor markers.
研究领域:
生物医学工程
·
材料科学
·
临床检验
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Shendong Liu,Danxuan Lin,Yunping Lai,Li Hou,Tianran Lin,Shulin Zhao
By combining the photothermal properties of the 3,3′,5,5′-tetramethylbenzidine oxidation product (TMBox) with the sensitive quenching of perovskite fluorescence by ammonia gas, a gas-mediated immunoassay at atmospheric pressure was constructed, which took the fluorescence quenching length of perovskite fluorescent capillary as the signal output. First, a CsPbBr3 perovskite with surface modification of 3-aminopropyl triethoxysilane was synthesized by thermal injection and decorated to the capillary wall by glutaraldehyde cross-linking. In the presence of H2O2 and the tumor marker carcinoembryonic antigen (CEA), TMB was oxidized to TMBox by the horseradish peroxidase (HRP)-labeled CEA antibody. The photothermal effect of TMBox at 808 nm laser irradiation increases the concentration of ammonia gas, and the prepared fluorescent capillary can respond sensitively to ammonia gas. The fluorescence quenching length can be observed by the naked eye for a semiquantitative evaluation of CEA concentration. At the same time, we developed a mobile APP for the first time to measure the fluorescence quenching length. In the range of 0–20 ng mL–1, the quenching length increased linearly with the increase in CEA concentration, and the detection limit was 0.078 ng mL–1. This method has been successfully used for the detection of CEA in human serum with a recovery of 95.8%–106.5%.
研究领域:
生物化学
·
医学检测
·
光学传感
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DOI
PubMed
高影响因子
TALANTA
·
2015
·
IF 3.545
Juan Li,Hangqing Xie,Yuhong Liu,Hang Ren,Wenbo Zhao,Xiaohua Huang
Nanomaterial-based signal-amplification strategies hold a great promise in realizing sensitive biological detection. A simple label-free electrochemical immunosensor for sensitive detection of carcinoembryonic antigen (CEA) was developed by immobilizing anti-CEA antibodies onto the Au-F127 strawberry-like nanospheres modified glassy carbon electrode (Au-F127/GCE). The Au-F127 strawberry-like nanospheres offered a large surface and multifunctional substrate for the effective immobilization of anti-CEA and the existence of Au could accelerate electron transfer and make the electrochemical signal amplified. The Au-F127 nanocomposites and anti-CEA were characterized by transmission electron microscopy (TEM), polycrystalline electron diffraction ring pattern, ultra-violet visible (UV–vis) spectra and attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra. Electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were employed to verify the stepwise assembly of the immunosensor and evaluated the analytical performance of the fabricated immunosensor, respectively. The immunosensor showed a wide liner response range between 0.01 and 80 ng mL −1 with a low detection limit of 0.24 pg mL −1 at a signal-to-noise (S/N) ratio of 3. Additionally, the proposed method was successfully applied to determine CEA in human serum samples with satisfactory results.
研究领域:
生物医学工程
·
分析化学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 5
·1353-1360
·
IF 4.58
Liu Qiao,Liu Xing-Pei,Wei Yu-Pin,Mao Chang-Jie,Niu He-Lin,Song Ji-Ming,Jin Bao-Kang,Zhang Sheng-Yi
CdSe:Eu nanocrystals were successfully synthesized and characterized by transmission electron microscopy, X-ray powder diffraction, and X-ray photoelectric spectroscopy. The CdSe:Eu nanocrystals showed enhanced green electrochemiluminescence (ECL) intensity when compared to pure CdSe nanocrystals. Further, the nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen (CEA) that has a linear response over the 1.0 fg·mL −1 to 100 ng·mL −1 CEA concentration range with a 0.4 fg·mL −1 detection limit. The assay was applied to the determination of CEA in human serum samples.
Schematic of the assay: GCE-glassy-carbon electrode, Ab- Antibody, BSA- Bovine serum albumin, Ag- Antigen. CdSe:Eu nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen.
研究领域:
纳米技术
·
分析化学
·
生物医学工程
·
临床诊断
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）