Journal of Pharmaceutical Analysis
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2023
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IF 8.8
Yang Yi,Wenzhe Li,Kefang Liu,Heng Xue,Rong Yu,Meng Zhang,Yang-Oujie Bao,Xinyuan Lai,Jingjing Fan,Yuxi Huang,Jing Wang,Xiaomeng Shi,Junhua Li,Hongping Wei,Kuanhui Xiang,Linjie Li,Rong Zhang,Xin Zhao,Xue Qiao,Hang Yang,Min Ye
Currently, human health due to corona virus disease 2019 (COVID-19) pandemic has been seriously threatened. The coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein plays a crucial role in virus transmission and several S-based therapeutic approaches have been approved for the treatment of COVID-19. However, the efficacy is compromised by the SARS-CoV-2 evolvement and mutation. Here we report the SARS-CoV-2 S protein receptor-binding domain (RBD) inhibitor licorice-saponin A3 (A3) could widely inhibit RBD of SARS-CoV-2 variants, including Beta, Delta, and Omicron BA.1, XBB and BQ1.1. Furthermore, A3 could potently inhibit SARS-CoV-2 Omicron virus in Vero E6 cells, with EC 50 of 1.016 μM. The mechanism was related with binding with Y453 of RBD determined by hydrogen-deuterium exchange mass spectrometry (HDX-MS) analysis combined with quantum mechanics/molecular mechanics (QM/MM) simulations. Interestingly, phosphoproteomics analysis and multi fluorescent immunohistochemistry (mIHC) respectively indicated that A3 also inhibits host inflammation by directly modulating the JNK and p38 MAPK pathways and rebalancing the corresponding immune dysregulation. This work supports A3 as a promising broad-spectrum small molecule drug candidate for COVID-19.
研究领域:
病毒学
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药理学
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免疫学
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分子生物学
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PubMed
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2023
·
IF 11.4
Shengchao Hu,Shangbiao Fang,Jian Zhao,Guowei Wang,Weixiao Qi,Gong Zhang,Cunrui Huang,Jiuhui Qu,Huijuan Liu
Antiviral transformation products (TPs) generated during wastewater treatment are an environmental concern, as their discharge, in considerable amounts, into natural waters during a pandemic can pose possible risks to the aquatic environment. Identification of the hazardous TPs generated from antivirals during wastewater treatment is important. Herein, chloroquine phosphate (CQP), which was widely used during the coronavirus disease-19 (COVID-19) pandemic, was selected for research. We investigated the TPs generated from CQP during water chlorination. Zebrafish (Danio rerio) embryos were used to assess the developmental toxicity of CQP after water chlorination, and hazardous TPs were estimated using effect-directed analysis (EDA). Principal component analysis revealed that the developmental toxicity induced by chlorinated samples could be relevant to the formation of some halogenated TPs. Fractionation of the hazardous chlorinated sample, along with the bioassay and chemical analysis, identified halogenated TP387 as the main hazardous TP contributing to the developmental toxicity induced by chlorinated samples. TP387 could also be formed in real wastewater during chlorination in environmentally relevant conditions. This study provides a scientific basis for the further assessment of environmental risks of CQP after water chlorination and describes a method for identifying unknown hazardous TPs generated from pharmaceuticals during wastewater treatment.
研究领域:
环境科学
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毒理学
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药物化学
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水生生物学
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PubMed
高影响因子
ADVANCED MATERIALS
·
2021
·Vol. 33
·期 34
·2101707
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IF 30.849
Min Li,Sanpeng Li,Yixuan Huang,Haixia Chen,Songya Zhang,Zhicheng Zhang,Weigang Wu,Xiaobin Zeng,Boping Zhou,Bin Li
The transfer of foreign synthetic messenger RNA (mRNA) into cells is essential for mRNA-based protein-replacement therapies. Prophylactic mRNA COVID-19 vaccines commonly utilize nanotechnology to deliver mRNA encoding SARS-CoV-2 vaccine antigens, thereby triggering the body's immune response and preventing infections. In this study, a new combinatorial library of symmetric lipid-like compounds is constructed, and among which a lead compound is selected to prepare lipid-like nanoassemblies (LLNs) for intracellular delivery of mRNA. After multiround optimization, the mRNA formulated into core–shell-structured LLNs exhibits more than three orders of magnitude higher resistance to serum than the unprotected mRNA, and leads to sustained and high-level protein expression in mammalian cells. A single intravenous injection of LLNs into mice achieves over 95% mRNA translation in the spleen, without causing significant hematological and histological changes. Delivery of in-vitro-transcribed mRNA that encodes high-affinity truncated ACE2 variants (tACE2v mRNA) through LLNs induces elevated expression and secretion of tACE2v decoys, which is able to effectively block the binding of the receptor-binding domain of the SARS-CoV-2 to the human ACE2 receptor. The robust neutralization activity in vitro suggests that intracellular delivery of mRNA encoding ACE2 receptor mimics via LLNs may represent a potential intervention strategy for COVID-19.
研究领域:
纳米技术
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分子生物学
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药学
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病毒学
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治疗学
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PubMed
高影响因子
Frontiers in Psychology
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2021
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IF 2.988
Alexander S. English,Xiaoyuan Li
COVID-19 has drastically altered people’s mask-wearing behaviors around the world. What is unknown is how long these mask behaviors will last post-COVID-19? To investigate how individual, situational, and locational factors influence mask use in the absence of community spread of COVID-19, we conducted an observational study in public areas in the megacity of Shanghai, China. Researchers coded people’s mask use in various suburban and urban districts and outdoor and indoor locations with and without mask requirements. Firstly, even without any local transmissions in more than 40days, 62% of the sample (N=1,282) still wore masks in public places. The data showed that people in more urban areas wore masks more often and that people wore masks in places where it was mandated. Women also wore masks more than men, and older people complied more with mask enforcement policies. We found that more densely populated districts and areas with more inflow of non-locals also predicted more mask use. We argue that the pandemic has long-lasting effects on human behavior like mask usage and reflects individuals’ continual conformity to new social norms.
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高影响因子
International Journal of Modeling Simulation and Scientific Computing
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2021
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IF 0
Xingzhi Chen,Baodan Tian,Feixiang Huang,Daiwen Wang,Linyan Fu,Haoying Xu
This paper analyzes the spread of the COVID-19 epidemic in China, establishes a SEIR + CAQ epidemiologic model that classifies the diagnosed population into asymptomatic, mild, and severe, and considers the condition of an isolated population. First of all, we theoretically analyzed the key problems of the disease-free equilibrium, stability, and basic reproduction number of the model. Then, combined with the actual data, the development trend of the epidemic situation is simulated and compared, and the influence of some important parameters is discussed in the model evaluation part. Finally, based on the theoretical analysis and numerical simulation results, we forecast the future development of the COVID-19 epidemic and put forward some reasonable scientific suggestions for the government to formulate a future epidemic prevention policy.
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DOI
International Journal of Environmental Research and Public Health
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2023
·Vol. 20
·期 3
·2068
·
IF 4.614
Xiaoran Huang,Demiao Yu
The Omicron variant of COVID-19, which emerged at the end of 2021, has caused a new wave of infections around the world and is causing a new wave of the crisis due to the extreme variability of the pathogen. In response to public health emergencies such as SARS and COVID-19, the first task is to identify the vulnerabilities of regional health systems and perform a comprehensive assessment of the region’s resilience. In this paper, we take the carrying capacity of medical resources as the focus; evaluate the medical, human, and financial resources of various regions; and construct an epidemic safety index based on the actual situation or future trend of the epidemic outbreak to evaluate and predict the risk level of each region in response to the epidemic. The study firstly evaluates the epidemic safety index for each province and city in China and 150 countries around the world, using the first wave of the COVID-19 epidemic in 2020 and the Omicron variant virus in 2022 as the background, respectively, and justifies the index through the actual performance in terms of epidemic prevention and control, based on which the epidemic safety index for 150 countries in the next year is predicted. The conclusions show that Europe, the Americas, and parts of Asia will face a significant risk of epidemic shocks in the coming period and that countries need to formulate policies in response to the actual situation of the epidemic.
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高影响因子
ELECTROCHIMICA ACTA
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2022
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IF 7.336
Cuilin Meng,Qiongfang Zhuo,Anqi Wang,Jingjing Liu,Zehong Yang,Junfeng Niu
In this study, TiO 2 nanotube arrays (TiO 2 -NTA) were used as an intermediate layer to provide effective electrocatalytic activity and stability for Ti/TiO 2 -NTA/Ti 4 O 7 anode. Compared with Ti/Ti 4 O 7 anode, Ti/TiO 2 -NTA/Ti 4 O 7 anode exhibited higher oxygen evolution potential (2.40 V), larger active specific surface areas (1.81 m 2 g −1 ), stronger radical generation capacity (64.42 μM), longer accelerated service life (56.0 h), and superior favipiravir removal ratio. The flow-through electrochemical reaction system was constructed by using the porous Ti/TiO 2 -NTA/Ti 4 O 7 anode. The removal ratio, TOC removal ratio and mineralization current efficiency of favipiravir in the flow-by electrochemical system were significantly improved and the energy consumption was reduced compared with the conventional flow-by electrochemical system, verifying the superiority of the porous flow-through Ti/TiO 2 -NTA/Ti 4 O 7 anode. The effects of operating parameters on the removal of favipiravir in the flow-through electrochemical reaction system were investigated. The degradation mechanisms of favipiravir are the synergetic effects of the free radical (·OH and SO 4 − ·) and the direct electron transfer. Ti/TiO 2 -NTA/Ti 4 O 7 anode displayed excellent stability in five consecutive cycles, exhibited significant removal ratio (87.7%) of favipiravir from actual wastewater, and remained efficient and versatile for a wide range of typical PPCPs pollutants. Therefore, Ti/TiO 2 -NTA/Ti 4 O 7 is a promising porous anode material in the engineering application.
研究领域:
环境科学
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电化学工程
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药物处理
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高影响因子
CARBOHYDRATE POLYMERS
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2022
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IF 10.723
Lihua Geng,Quanbin Zhang,Qishan Suo,Jing Wang,Yingxia Wang,Cong Wang,Ning Wu
COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis . NMR analysis showed OP145 was mainly composed of →3)-β- d -Gal-(1 → 4)-α- l -Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-α- l -Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 μg/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19.
研究领域:
病毒学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
Vaccines
·
2021
·Vol. 9
·期 5
·443
·
IF 4.422
Kaiyi Han,Mark R. Francis,Ruiyun Zhang,Qian Wang,Aichen Xia,Linyao Lu,Bingyi Yang,Zhiyuan Hou
Understanding the public’s attitude towards COVID-19 vaccination and their acceptance could help facilitate the COVID-19 rollout. This study aimed to assess the acceptance and willingness to pay (WTP) for the COVID-19 vaccine among migrants in Shanghai, China. A cross-sectional study was conducted among 2126 migrants in Shanghai for the period 1–20 November 2020. Convenience sampling was used to recruit respondents in workplaces with large numbers of migrant workers. Multivariable (ordered) logistic regressions were used to examine factors associated with acceptance and WTP of the COVID-19 vaccine. Most (89.1%) migrants would accept COVID-19 vaccination. Over 90.0% perceived the COVID-19 vaccine as important, while only 75.0% and 77.7% perceived vaccines safe and effective. Socio-demographic factors were not significantly associated with vaccine acceptance, but confidence in the importance (OR 8.71, 95% CI 5.89–12.89), safety (OR 1.80, 95% CI 1.24–2.61) and effectiveness (OR 2.66, 95% CI 1.83–3.87) of COVID-19 vaccine was significantly positively associated with vaccine acceptance. The top reasons for vaccine hesitancy were lack of vaccine information and confidence. The proportion of those definitely willing to get the COVID-19 vaccine was 20% lower if paid by themselves than free vaccination. Migrants were willing to pay a median amount of USD 46 for the COVID-19 vaccine. Results show that a high acceptance of the COVID-19 vaccine was universal among migrants in Shanghai. Concerns about vaccine safety, effectiveness and high costs of the COVID-19 vaccine may hinder their uptake. Effective health communication to build confidence in the COVID-19 vaccine and subsidies toward the costs of these vaccines are needed to improve uptake.
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高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2022
·
IF 24.319
Yuan Jiang,Ruolan Xu,Chen Zeng,Kejie Wang,Lujia Han,Xuesong Zhang
This study explored a scalable decomposition-catalysis route to effectively valorize disposable COVID-19 face mask into tunable value-added products. The metal-doping carbonaceous catalysts (M/Cs) were successfully synthesized through a single-step energy-efficient thermal process. The modest decomposition-catalysis of face mask over Zn/C gave rise to a relatively high yield (73.52%) of liquid oil with 99% of selectivity toward jet fuel range (C 8 – C 16 ) hydrocarbons. Besides, the scalable decomposition-catalysis of face mask over Ni(50)/C at 800 ℃ was observed to thoroughly transform face mask into gas with the cumulative gas yield of 967 NmL/g mask , involving 49.90 vol% of H 2 and 40.90 vol% of CH 4 . Moreover, a dense, entangled growth of carbon nanotubes were formed on the surface of M/Cs. In brief, this study developed a scalable and promising route by using M/Cs for the valorization of COVID-19 face mask into hydrocarbon fuel or coupling harvest of H 2 -enriched fuel gas and carbon nanotubes.
研究领域:
材料科学
·
环境科学
·
化学工程
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DOI
高影响因子
Gut Microbes
·
2022
·
IF 9.434
Liqiu Jia,Shufeng Weng,Jing Wu,Xiangxiang Tian,Yifan Zhang,Xuyang Wang,Jing Wang,Dongmei Yan,Wanhai Wang,Fang Fang,Zhaoqin Zhu,Chao Qiu,Wenhong Zhang,Ying Xu,Yanmin Wan
The origins of preexisting SARS-CoV-2 cross-reactive antibodies and their potential impacts on vaccine efficacy have not been fully clarified. In this study, we demonstrated that S2 was the prevailing target of the preexisting S protein cross-reactive antibodies in both healthy human and SPF mice. A dominant antibody epitope was identified on the connector domain of S2 (1147-SFKEELDKYFKNHT-1160, P144), which could be recognized by preexisting antibodies in both human and mouse. Through metagenomic sequencing and fecal bacteria transplant, we demonstrated that the generation of S2 cross-reactive antibodies was associated with commensal gut bacteria. Furthermore, six P144 reactive monoclonal antibodies were isolated from naïve SPF mice and were proven to cross-react with commensal gut bacteria collected from both human and mouse. A variety of cross-reactive microbial proteins were identified using LC-MS, of which E. coli derived HSP60 and HSP70 proteins were confirmed to be able to bind to one of the isolated monoclonal antibodies. Mice with high levels of preexisting S2 cross-reactive antibodies mounted higher S protein specific binding antibodies, especially against S2, after being immunized with a SARS-CoV-2 S DNA vaccine. Similarly, we found that levels of preexisting S2 and P144-specific antibodies correlated positively with RBD binding antibody titers after two doses of inactivated SARS-CoV-2 vaccination in human. Collectively, our study revealed an alternative origin of preexisting S2-targeted antibodies and disclosed a previously neglected aspect of the impact of gut microbiota on host anti-SARS-CoV-2 immunity.
研究领域:
免疫学
·
微生物学
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分子生物学
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PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Hai-Chen Tu,Ling-Xi Zhao,Lu Liu,Xiao-Xing Wang,Jin-Ming Lin,Xia Wang,Ru-Song Zhao
The consumption of famciclovir (FCV) has been increased dramatically since the outbreak of coronavirus in 2019, and the pollution and harm of FCV in waters are concerned. Here, by utilizing aryl halides on 2, 4, 6-tris(4-bromophenyl)− 1, 3, 5-triazine (BPT) and primary amine groups on benzidine (BZ), a novel conjugated microporous polymer, namely BPT-BZ-CMP, was synthesized by Buchwald–Hartwig coupling reaction and applied in the removal of FCV from aqueous solution firstly. The synthesized BPT-BZ-CMP were characterized by various methods, including FTIR, SEM, BET, and Zeta–potential. Due to the micropore structure and high specific surface area, it took only 30 min for BPT-BZ-CMP to adsorb FCV to reach an equilibrium, and the maximum adsorption capacity was 347.8 mg·g −1 . The Liu and pseudo-second-order kinetic models properly fit the adsorption equilibrium and kinetic data, respectively. The adsorption process was a spontaneous process, and the hydrogen bonding, π–π interaction and C–H···π interaction enhanced the adsorption of FCV on BPT-BZ-CMP. BPT-BZ-CMP maintained a good adsorption capacity after four consecutive adsorption–desorption cycle experiments. This study confirmed the potential of BPT-BZ-CMP as efficient sorbent to remove FCV from aqueous solutions.
研究领域:
环境科学
·
材料科学
·
药物化学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）