Food Chemistry-X
·
2024
·
IF 6.5
Peng Wang,Haifan Wang,Yang Xiao,Jialiang Zou,Hongping Chen,Lin Chen,Fu Wang,Yuan Hu,Youping Liu
In this study, comprehensive and systematic nontargeted metabolomics analysis was performed with the metabolites of Zangju peel ( Citrus reticulata cv. Manau Gan, CRZP, which has been cultivated for over 400 years in Derong County, China.) at four different mature stages. A total of 1878 metabolites were identified, among which flavonoids were the most abundant (62.04 %), and identified 62 key differential metabolites significantly affected by maturity. Based on biological activity measurements, CRZP showed better antioxidant activity, lipase inhibition ability, inhibition of adipogenic differentiation in 3TT-L1 cells and promotion of lipid metabolism, with the biological activity of CRZP at different maturity stages being associated with key differential metabolite. Thus, CRZP is natural antioxidants and possess anti-obesity potential, and industrial production needs to consider the Maturity stage of its collection.
研究领域:
植物科学
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代谢组学
·
药理学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2021
·
IF 4.27
Yu-Feng Xie,Rong-Xiang Zhang,Li-Jun Qin,La-la Song,De-Gang Zhao,Zhong-min Xia
MYB proteins play a crucial role in plant growth and development and stress responses. In this study, 160 members of the MYB gene family from the pepper genome database were used to analyze gene structures, chromosome localization, collinearity, genetic affinity and expression in response to heavy metals . The results identified R2R3-MYB members and further phylogenetically classified them into 35 subgroups based on highly conserved gene structures and motifs. Collinearity analysis showed that segmental duplication events played a crucial role in the functional expansion of the CaMYB gene family by intraspecific collinearity, and at least 12 pairs of CaMYB genes existed between species prior to the differentiation between monocots and dicots . Moreover, the upstream CaMYB genes were mainly localized to the phytohormone elements ABRE and transcription factor elements MYB and MYC. Further analysis revealed that MYB transcription factors were closely associated with a variety of abiotic stress-related proteins (e.g., MAC-complex and SKIP). Under the stress of five metal ions, Cd 2+ , Cu 2+ , Pb 2+ , Zn 2+ , and Fe 3+ , the expression levels of some CaMYB family genes were upregulated. Of these genes, pairing homologous 1 ( PH-1 ), PH-13 , and PH-15 in the roots of Capsicum annuum were upregulated to the greatest extent, indicating that these three MYB family members are particularly sensitive to these five metals. This study provides a theoretical reference for the analysis of the molecular regulatory mechanism of MYB family genes in mediating the response to heavy metals in plants. This study reveals the mode of interaction between MYB and a variety of abiotic stress proteins and clarifies the biological functions of CaMYB family members in the regulation of heavy metal stress.
研究领域:
植物学
·
遗传学
·
环境科学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Hui Zhang,Xia Deng,Junrong Fu,Ye Chen,Shiwei Jin
Surface-enhanced Raman scattering (SERS) has been widely applied in the field of food safety due to its exceptional molecular recognition capabilities. In this study, a simple method for preparing a flexible SERS substrate was designed for the ultrasensitive real-time detection of the banned drugs malachite green (MG) and crystal violet (CV). The substrate was prepared by patterned spraying of a superhydrophobic coating prepared from candle soot onto the surface of a poly(vinyl alcohol) (PVA) sponge, followed by simple Ag nanoparticle (Ag NP) deposition in the superhydrophilic region. The PVA sponge with special hydrophilicity can completely absorb the droplets to be measured within 1–5 s without additional drying treatment. And the Ag NPs aggregated by potassium iodide (KI) resulted in a significant enhancement of the Raman signal, enabling the substrate to realize the detection of MG and CV as low as 100 fg/mL (2.74 × 10–13 M) and 1 pg/mL (2.45 × 10–12 M), respectively, with a long lifetime (90 days). Thanks to the excellent mechanical properties of the flexible substrate, the substrate can realize the simultaneous detection of very small amounts of MG and CV on the surface of crayfish by simple wiping, which has great application prospects in the field of food safety and field inspection.
研究领域:
分析化学
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材料科学
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食品安全
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纳米技术
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产品关联
DOI
高影响因子
Food & Function
·
2021
·Vol. 12
·期 19
·9300-9314
·
IF 5.396
Xiao Guo,Xuedan Cao,Xiugui Fang,Ailing Guo,Erhu Li
In this study, Ougan juice (OJ) and lactic acid bacteria fermented Ougan juice (FOJ) were investigated individually for their capability of preventing obesity in high-fat diet (HFD)-fed C57BL/6J mice. After being administered with OJ or FOJ for 10 weeks, the body weight gain, hyperlipidemia, and gut microbiota dysbiosis of HFD-fed mice were examined. The results showed that OJ or FOJ supplementation inhibited weight gain, lowered fat accumulation, reduced liver steatosis, improved glucose homeostasis and insulin sensitivity, increased brown adipose tissue (BAT) activity, and promoted white adipose tissue (WAT) browning. Both OJ and FOJ additions increased the diversity of gut microbiota. OJ reduced the relative abundance of phylum Erysipelatoclostridiaceae and genus Erysipelatoclostridium and remarkably increased SCFA-producing bacteria Blautia, while FOJ reduced the ratio of Firmicutes to Bacteroidetes and enhanced the relative abundance of family Lactobacillaceae. Spearman's correlation analysis revealed that Akkermansia, Dubosiella, and Muribaculaceae were significantly negatively correlated with obesity-related indexes. In general, FOJ exhibited a better inhibitory effect on obesity than OJ, and the possible inhibitory mechanism lies in promoting WAT browning and increasing intestinal probiotics. This study provides the guidance for developing fermented Ougan juice as an obesity-inhibiting functional food.
研究领域:
营养学
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肥胖研究
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胃肠病学
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微生物学
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代谢学
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DOI
PubMed
高影响因子
Polymers
·
2022
·Vol. 14
·期 3
·536
·
IF 4.967
Muhammad Rafiullah Khan,Chongxing Huang,Rafi Ullah,Hakim Ullah,Ihsan Mabood Qazi,Taufiq Nawaz,Muhammad Adnan,Abdullah Khan,Hongxia Su,Liu Ren
Plant extracts represent a rich repository of metabolites with antioxidant and antimicrobial properties. Neem (Azadirachta indica) is a medicinal plant considered the tree of the 21st century. In this study, we investigated the antioxidant and antimicrobial effects of propyl disulfide (PD), a major volatile compound in neem seed, against the pericarp browning (BI), microbial decay incidence (DI), and water loss of longan fruit. Fresh longan cv. Shixia samples were packaged in oriented polypropylene (OPP) and polyethene (PE) packages of different thicknesses (20, 40, and 60 µm). Sterile gauze was fixed inside the packages and 500 uL of PD was placed on them to avoid the direct contact of PD with fruit samples. Packages were sealed immediately to minimize vaporization and stored at 12 ± 1 °C for 18 days. Fruit samples packaged in open net packages served as controls. The results showed that fruit treated with PD in OPP and PE packages significantly prevented losses of water, DI, and BI compared to control treatment. PD also maintained the color, TSS values, TA values, pH values, high peel firmness, high TPC content, and high TFC content, and reduced the activity levels of PPO and POD. Scanning electron microscope (SEM) analysis indicated that the exocarp, mesocarp, and endocarp of longan peel were smooth, uniform, and compact with no free space compared to control, where crakes, a damaged and loose structure, and a lot of fungal mycelia were found. The shortest shelf life of 9 days was observed in control as compared to 18 days in OPP-20 and OPP-40; 15 days in OPP-60, PE-20, and PE-40; and 12 days in PE-60 packaging films. Therefore, PD as a natural antioxidant and antimicrobial agent, in combination with OPP-20 and OPP-40 polymeric films, could successfully be applied commercially to extend the postharvest shelf life of longan.Keywords:longan;fruit;polymeric films;antioxidant activity;enzymatic browning;neem;propyl disulfide;microbial decay;essential oil
研究领域:
食品科学
·
植物学
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产品关联
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2026
·
IF 4.6
Wenjing Liu,Lijuan Fu,Lihua Ren,Xiangxin Lin,Jia Guo,Jianwei Fu,Sun'an Yan
Lindl. cv. 'Furong' is a characteristic advantageous fruit of Fujian Province, China. Yet its systematic chemical basis of quality traits and the quality differences among core producing areas remain unclear. Here, Furong plum samples from five core producing areas were analyzed using high-performance liquid chromatography, headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry, and ultra-high performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry to characterize the chemical aroma composition and elucidate metabolic characteristics and quality variations among producing areas. Results showed that total soluble sugars in Furong plum were dominated by fructose (3.12∼3.84 g/100 g FW), followed by glucose and sucrose. Organic acids were composed predominantly of malic acid (418.73∼630.43 mg/100 g FW). The protein content, vitamin C content, total polyphenol content and total flavonoid content ranged from 0.65 to 0.71 g/100 g FW, 5.53 to 6.67 mg/100 g FW, 0.41 to 0.59% and 0.29 to 0.39%, respectively. A total of 30 volatile compounds and 342 non-volatile metabolites were identified. Among them, volatile compounds were dominated by esters and alcohols, while non-volatile metabolites primarily consisted of flavonoids, phenolic acids and organic acids. (E)-3-hexen-1-yl acetate, hexyl ethanoate, hexanal, 1-hexanol and (Z)-3-hexen-1-ol were identified as potential aroma-related compounds based on their high estimated odor activity values from multivariate analysis. Differentially accumulated metabolites were mainly concentrated in flavonoids (procyanidin B5, kaempferol 3-O-β-rutinoside, quercetin-3-O-deoxyhexoside), phenolic acids (5-O-caffeoylquinic acid, 5-formylsalicylic acid) and organic acids (malic acid, citric acid). This study preliminarily elucidated the metabolic network underlying flavor formation in Furong plum and the core driving factors for quality differences among its producing areas.
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产品关联
DOI
高影响因子
TALANTA
·
2024
·
IF 5.6
Xishun Peng,Qixin chen,Xinghua Li,Li Yang,Yiheng Yuan,Cheng Zuo,Zhangyu Zhou,Zhongchen Bai
Crystal violet (CV) residues in Marine food have produced a severe health threat in human life. In this study, we proposed a semiconductor surface-enhanced Raman scattering (SERS) sensor of corrosion-resistant Polyaniline/Graphene oxide (PPy/GO) film by electrochemical growth method to detect CV residues in fresh fish tissue. A PPy/GO dispersion solution was one-step deposited on a stainless steel sheet surface by electrochemical polymerization process to form a PPy/GO composite film acting as a semiconductor SERS substrate. Since the substrate of PPy/GO film was mainly composed of GO sheet without other metals, it had a good corrosion resistance. The SERS enhancement factor and charge transfer intensity P CT of PPy/Go SERS substrate for CV molecules were up to 1.18 × 10 6 and 0.903, respectively. Furthermore, the limit of detection ( LOD ) of PPy/GO SERS substrate could reach 1.58 nM. In addition, SERS sensor of PPy/GO film could identify CV residues in fresh fish tissues, and its recovery rate was 91.8 %–107 %. This preparing method and detecting method we proposed PPy/GO SERS substrate provide a new pathway for detecting CV residues in Marine food.
研究领域:
材料科学
·
分析化学
·
环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
Nanophotonics
·
2019
·Vol. 8
·期 10
·1719-1729
·
IF 6.908
Xiaofei Zhao,Chonghui Li,Zhen Li,Jing Yu,Jie Pan,Haipeng Si,Cheng Yang,Shouzhen Jiang,Chao Zhang,Baoyuan Man
An efficient electrospun aligned surface enhanced Raman scattering (SERS) and maize-like substrate of polyvinyl alcohol (PVA) composite and Ag colloid nanofibers decorated with thermal evaporated Ag nanoparticles (AgNPs) has been developed by taking advantage of electrostatic interactions. The synergistic effects of the evaporated AgNPs (niblets) and the Ag colloid in PVA (corncob) could arouse strong electromagnetic field between the lateral and vertical nanogaps which has been demonstrated by experiment and finite-different time-domain (FDTD) simulation. In this experiment, the aligned nanofibers possesses an excellent sensitivity by detection of crystal violet (CV) and malachite green (MG) molecule at low concentration. Moreover, the proposed flexible SERS sensor was measured with outstanding uniformity and reproducibility. We also carried out in-situ electrospinning on a curved surface to detect the mixture of Sudan I, CV and MG molecule, which demonstrates that flexible SERS sensor, has enormous potential in accurate and in-situ detection on the complex geometric structure.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
表面科学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Na Li,Junren Xu,Yiqiao Zhao,Mengmeng Zhao,Zhonghua Liu,Kunbo Wang,Jianan Huang,Mingzhi Zhu
Tea polyphenols transform under processing methods, but a systematic study on their changes in the same large-leaf tea cultivar is lacking. Here, Camellia sinensis var. assamica cv. Yunkang-10 leaves underwent six processing methods and were assessed using optimized nontargeted (UHPLC-Q-Exactive Orbitrap-MS) and targeted (UHPLC-QqQ-MS) polyphenomics, along with molecular networking analysis. 903 and 52 polyphenolic compounds (catechins, flavones and flavonols, and phenolic acids) were respectively relatively and absolutely quantified for the first time. Dark and black teas, with the lowest polyphenol content, differed from the other four tea types, although variations existed among these four teas. However, some flavonol and flavone aglycones (e.g. kaempferol, apigenin), as well as some phenolic acids (e.g. ellagic acid, gallic acid), exhibited higher levels in dark and black teas. Correlations between polyphenolic composition and electronic sensory characteristics were observed using E-tongue and E-eye. This study enriches understanding of polyphenol profiles in Chinese teas post diverse processing.
研究领域:
食品科学
·
植物化学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2026
·
IF 5.5
Huanhuan Xu,Jialiang Zou,Xuelian Gan,Fu Wang,Lin Chen,Hongping Chen,Yuan Hu,Youping Liu
Introduction Zhoupigan ( Citrus reticulata cv. Manau Gan), also known as Zangju or Shitougan, is a distinctive Chinese citrus resource valued for both traditional medicinal uses and food flavoring, and is notably rich in essential oils (EOs). However, the chemical composition and bioactivity of Zhoupigan peel essential oil (ZJPEO) remain insufficiently understood, limiting its effective utilization. Methods In this study, ZJPEO was extracted by steam distillation (SD) and supercritical fluid extraction (SFE) and analyzed using FTIR, E-nose, HS-GC-IMS and HS-SPME-GC-MS. And its antioxidant and antimicrobial activities were evaluated in vitro . Results FTIR and E-nose revealed clear compositional differences between steam-distilled essential oil (SDEO) and supercritical fluid-extracted essential oil (SFEO). HS-GC-IMS detected 63 VOCs and, based on VIP > 1, identified five marker compounds. HS-SPME-GC-MS identified 1,250 VOCs, showing terpenoids and esters as major contributors to aroma and bioactivity. KEGG enrichment highlighted sesquiterpene and triterpene biosynthesis as key pathways. Flavor wheel analysis indicated that SFEO had stronger sweet, green, and woody notes and exhibited potent antioxidant and antimicrobial activities associated with specific VOCs. Discussion Overall, this work supports optimizing ZJPEO extraction and promoting its value-added, sustainable utilization.
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产品关联
DOI
PubMed
高影响因子
IONICS
·
2024
·
IF 2.4
Zheng Tingting,Wang Zhixing,Wang Siwu,Li Xinhai,Guo Huajun,Peng Wenjie,Wang Jiexi,Yan Guochun
Spinel LiMn 2 O 4 -based lithium-ion batteries are widely applied in electric two-wheeler and low-speed vehicles due to their low cost and low toxicity. Nevertheless, the Mn deposition at the anode originated from Mn dissolution of LiMn 2 O 4 causes a poor elevated temperature cycle life of graphite/LiMn 2 O 4 batteries. Herein, a graphite/LiFePO 4 cell with specific Mn 2+ added in the electrolyte is provided as an efficient evaluation method to research Mn 2+ deposition. Besides, an effective strategy by adding a ligand hexamethylenetetramine (HMTA) as an electrolyte additive is proposed to prevent Mn 2+ depositing. HMTA effectively “locks in” Mn 2+ through strong coordination, as confirmed by Raman spectroscopy and density functional theory (DFT) calculations. Moreover, the CV and XPS results indicate that the adsorption of HMTA is able to significantly inhibit side reactions at the anode/electrolyte interface. By adopting this strategy, the capacity retention of graphite/LiMn 2 O 4 cell is enhanced from 68.9% to 79.0% at 0.5 C after 100 cycles under 60 °C when adding 0.5% HMTA additive into the blank electrolyte. This work provides a new insight into revealing the mechanism of Mn 2+ regulation by introducing HMTA and improving the electrochemical performance of graphite/LiMn 2 O 4 batteries at elevated temperature.
研究领域:
材料科学
·
电化学
·
电池技术
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产品关联
DOI
高影响因子
Journal of Materials Chemistry A
·
2024
·
IF 10.7
Jingya Liu,Xin Xu,Hongkang Wang,Peng-Fei Wang,Kai Wu,Yonghong Cheng,Bing Xiao
The ammonization treatment is applied to a prototype ordered double-transition metal MXene known as Mo2TiC2Tx at different annealing temperatures (400, 500 and 600 °C) and durations (1 h and 2 h). The lattice structures, micro-morphologies, and chemical compositions of the resulting ammoniated MXenes are characterized by XRD, SEM, TEM, XPS and EPMA. All ammoniated MXenes preserve the layered microstructure of the parent carbide 2D material, and N atoms are successfully doped into the lattice sites to form carbonitride MXenes, denoted as Mo2Ti(C1−δNδ)2Tx. The Hall measurements are carried out to evaluate the electrical conductivity of ammoniated MXenes and raw Mo2TiC2Tx, and the results indicate that the carbonitride forms exhibit higher electrical conductivities and electron concentrations than the raw 2D material. The electrochemical properties of ammoniated MXenes are investigated by means of charge–discharge cycling CV tests, the GITT and EIS measurements at various scan rates. The ammoniated MXene sample (N400-1h) could deliver a storage capacity of 750 mA h g−1 (200 mA h g−1) at a current density of 0.03 A g−1 (2 A g−1). It is found that the capacitive charge storage mechanism through the intercalation reaction process dominates the total capacity for the ammoniated MXenes. The diffusion dynamics of Li+ in all investigated MXene electrodes show a two-stage lithiation mechanism in which the apparent diffusion coefficient is regulated by the different numbers of vacancies in the absorbate layers at various lithiation stages in the GITT curves. Overall, ammoniated MXenes also show superior performances in Li+ diffusion dynamics and charge transfer kinetics at the electrode–electrolyte interface to those of raw Mo2TiC2Tx, elucidating a new avenue for the use of ammoniated double-transition metal o-MXenes as high-performance electrode materials for LIBs and supercapacitors in the future.
研究领域:
材料科学
·
电化学
·
能量存储
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）