PHYTOCHEMICAL ANALYSIS
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2018
·Vol. 29
·期 6
·639-648
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IF 2.337
Yaqian Ma,Menghan Liu,Ting Tan,Aiping Yan,Lan Guo,Kun Jiang,Changheng Tan,Yiqun Wan
Introduction Camellia oleifera flowers are rich in flavonoids, but there has been little attention on their application. A simple and reliable method for determining the content of flavonoids in C. oleifera flowers would be very helpful for the utilisation of agriculture resources. Objective To develop an efficient analytical method for the determination of flavonoids in C. oleifera flowers by high-performance liquid chromatography-ultraviolet (HPLC-UV) detection. Methodology Preparing an environmentally-friendly and effective solvent – deep eutectic solvents (DESs) – for compound extraction. Then investigating the influential factors of ultrasound-assisted extraction with DESs by the Box–Behnken design combined with response surface methodology. Results DES-5 synthesised with choline chloride and lactic acid (1:2) acquired excellent extractability for four flavonoids (quercetin 3- O -rhamnoside, kaempferol 3- O -rhamnoside and their aglycones) with different polarity. The proposed method, which could simultaneously determine four flavonoids with HPLC-UV detection for the first time, displays satisfactory recovery yields and high precision with inter-day relative standard deviation lower than 5.80%. Conclusion DESs could be promising solvents for efficiently and selectively extracting bioactive compounds from plant materials, and the analytical method for flavonoids of C. oleifera flowers could provide reference value for its application and be used in other plant resources.
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PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Wang Yu,Haiyan Zhong,Xuezhi Fang,Menghao Du
This study aimed to assess the effects of industrial-scale oil-pressing techniques (hydraulic pressing (HP), twin-screw pressing (TP)), and organic solvent extraction (OE) on the physicochemical properties and antioxidant activities of protein isolates derived from Camellia ( Camellia oleifera Abel.) seed cakes. The predominant protein fractions in camellia seed cake were albumin (32–37 mg/g) and glutelin (19–27 mg/g), and the twin-screw pressed cake had a lower total protein content than the others. Spectral analysis showed that the α-helix content increased at the expense of random coil content in proteins from HP and TP cakes, indicating that extrusion altered the structure of the protein. The camellia seed protein particle size from HP and TP cakes was significantly reduced compared to that from the OE cake. The proteins obtained by HP and TP had stronger antioxidant activities than those obtained by OE. This research presents the potential for further applications of camellia seed cake protein to be utilized as antioxidants and emulsifiers in the food industries.
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FOOD CHEMISTRY
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2025
·
IF 9.8
Xinlian Zhao,Huihui Zhang,Shu Wang,Xinghe Zhang,Wu Zhong,Xingguo Wang,Pan Gao
Camellia oleifera seed oil (COSO) is highly susceptible to thermo-oxidative degradation due to its high content of unsaturated fatty acids, leading to nutrient loss and carcinogenic benzo[ a ]pyrene (BaP) formation. In this study, we employed a multimodal approach (Fourier transform infrared spectroscopy, FTIR; electron paramagnetic resonance, EPR; oxidation kinetics, α-tocopherol and BaP monitoring) to systematically compare four antioxidants (PG, TBHQ, BHT, VE) at industrially relevant concentrations. Results showed that TBHQ (0.2 g/kg) and PG (0.1 g/kg) optimally preserved α-tocopherols (6.70/4.58 mg/kg) and minimized BaP (1.40/1.46 μg/kg) during 15-day oxidation. FTIR revealed preferential stabilization of C H, cis-C=C, and C O bonds, while EPR confirmed superoxide radical (•O₂ − ) quenching via HAT/SET mechanisms. Kinetic analysis indicated higher activation energies for TBHQ (126.60 kJ/mol) and PG (92.63 kJ/mol), confirming enhanced stability. Mechanistically, TBHQ and PG function via phenolic hydroxyl H-transfer and quinonoid transformation. PG is a low-toxicity alternative to TBHQ for COSO stabilization.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2026
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IF 8.7
Meng He,Xian Yang,Mengfan Xu,Chao Liu,Binghua Yan
The valorisation of agricultural byproducts into functional biomaterials presents a crucial pathway towards a circular economy and precision agriculture. Herein, we report a closed-loop strategy to synthesise high value-added seed tape by integrating nanolignin from Camellia oleifera shells with chitosan matrices. Lignin is extracted from Camellia oleifera shells using a choline chloride–lactic acid deep eutectic solvent. Subsequently, size-controlled lignin nanoparticles (LNPs) are synthesised via an antisolvent method. These LNPs are incorporated into a chitosan (CS) matrix to prepare a film for agricultural seed tape. The lignin extraction demonstrated a high yield of 93.3% with almost-complete cellulose depletion, affording a guaiacyl–syringyl– p -hydroxyphenyl (G–S–H) type. The incorporation of LNPs in CS markedly enhanced the properties of the CS films. Specifically, the tensile strength of the CS/LNPs5 composite film (14 wt% LNPs) increased by 71.85% and its ultraviolet shielding capability reached 99.90%. A germination rate of 85% was achieved with the CS/LNPs4 (11 wt% LNPs) when the seed tape was tested for radish. Compared with treatments without LNPs, the CS/LNPs composites promote root and stem elongation. This study establishes a closed-loop biorefinery framework that converts lignocellulosic agricultural residues into biomass-derived functional materials.
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BIORESOURCE TECHNOLOGY
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2025
·
IF 9
Meng Li,Yangyang Chen,Yan Qing,Ming Liu,Yiqiang Wu,Lei Li
Camellia oleifera shell (COS), a lignin- and hemicellulose-rich cultivation byproduct, offers significant potential for resource extraction. In this study, we developed an integrated biorefinery approach using mild metal chloride-catalyzed hydrothermal pretreatment to vaporize COS waste. Under optimized conditions (0.03 M AlCl 3 , 170 °C, 60 min), hemicellulose-rich COS was selectively depolymerized into xylo -oligosaccharides (XOS) with a yield of 49.8 %. This efficient Lewis acid-catalyzed method produced high XOS yields and cellulose- and lignin-enriched solid residues. Compared to untreated samples, these residues, with reduced hemicellulose content, yielded hard carbon with increased interlayer spacing and closed pore structures, exhibiting superior sodium storage ability. This approach contrasts with traditional enzymatic hydrolysis and fermentation pathways. The hard carbon anode was incorporated into sodium-ion batteries, demonstrating practical feasibility with an initial reversible capacity (IRC) of 348 mAh g −1 , an initial coulombic efficiency (ICE) of 70.5 %, and capacity retention of 91.7 % after 100 cycles. This dual-product approach maximizes COS utilization and synergizes renewable chemical production with advanced energy storage, highlighting the unique advantages of our approach to XOS synthesis and material optimization, showcasing a clear advantage over existing single-product pathways.
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Antioxidants
·
2026
·Vol. 15
·期 7
·824
·
IF 8.2
Xiaoqing Feng,Xiao Zhou,Shushan Jia,Jingzhen Chen,Peiwang Li,Yan Yang,Wei Wu,Lijuan Jiang,Wenbin Zeng,Changzhu Li,Qiang Liu,Yunzhu Chen
Camelliasaponins, bioactive constituents abundant in the by-products ofCamellia oleiferaoil production, exhibit diverse biological activities. However, their potential in regulating neuroprotective mitophagy remains largely unexplored. This study identifies camelliasaponin B1 (CSB1) as an abundant component inC. oleiferaseeds and investigates its cytoprotective mechanisms against oxidative stress. Using an in vitro model of H2O2-induced oxidative damage in PC12 cells, we found that CSB1 pretreatment significantly alleviated oxidative stress, as evidenced by reduced reactive oxygen species (ROS) accumulation and enhanced antioxidant enzyme activities (SOD, CAT, GSH-Px). CSB1 also preserved mitochondrial function, restoring membrane potential (ΔΨm), ultrastructure, and respiratory capacity. Mechanistically, CSB1 reduces the expression of BNIP3/NIX-LC3B pathway-related proteins, suggesting a modulatory effect on mitophagy, as supported by transcriptomic analysis, Western blotting, and immunofluorescence. Molecular docking computationally predicted potential interactions between CSB1 and BNIP3/NIX proteins, which require experimental validation. Collectively, these findings suggest that CSB1 acts as a cytoprotective agent that enhances antioxidant defenses, safeguards mitochondrial integrity, and is associated with reduced BNIP3/NIX-LC3B expression and co-localization, offering a potential molecular basis for its development as a neuroprotective agent targeting oxidative stress-related mitochondrial dysfunction.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2017
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IF 3.615
Wentian Ma,Kexin Li,Huiqin Guo,Liushui Yan,Yuhua Dai,Xubiao Luo,Yong Yao
Porous carbon microspheres with numerous spherical microstructures were successfully fabricated directly from waste Camellia oleifera shells by hydrothermal carbonization combined with annealing treatment and potassium hydroxide chemical activation technique in the assistance of phloroglucinol. Numerous aromatic carbon microspheres were constructed after hydrothermal carbonization due to the structure-directing effect of phloroglucinol. The spherical microstructures remain intact after suffering from potassium hydroxide chemical activation because the aromatic carbon framework was further graphitized by annealing treatment. The mesoporous carbon microspheres were successfully obtained from microporous carbon microspheres through the secondary potassium hydroxide chemical activation. As valuable porous materials, the as-prepared microporous and mesoporous carbon microspheres were successfully applied to the sustained-release of anticancer drug 5-fluorouracil. The as-prepared microporous and mesoporous carbon microspheres showed some obvious advantages such as low-cost, high-capacity, and strong biological affinity compared with traditional drug sustained-release agent.
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Food Bioscience
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2025
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IF 5.9
Yixuan Wang,Songyu Yang,Shengbin Chen,Yingbing Zuo,Xiaoyu Mu,Hongmei Zhou,Yufei Yao,Xiaoping Peng,Wenjuan Li
This study aimed to explore the anti-tumor activity and mechanisms of the ethanol-soluble acidic components (ESAC) of Camellia oleifera cake through a combination of network pharmacology, in vitro and in vivo studies, and metabolomics based on UPLC-Q-TOF/MS. Additionally, the potential application of ESAC in combination with PD-1 inhibitors was explored. In this work, our results demonstrated that ESAC exhibited anti-tumor activities mediated by regulated cell death (RCD), which were associated with apoptosis, pyroptosis, and ferroptosis. UPLC-Q-TOF/MS-based serum metabolomics revealed that metabolic disorders were occurred in tumor group when compared to control group. In contrast, ESAC significantly inhibited abnormal energy metabolism including glycolysis, tricarboxylic acid cycle, and amino acid metabolism. Consequently, hexokinase and malate dehydrogenase, key enzymes of energy metabolism, were determined, and data showed that treatment of tumor mice with ESAC significantly suppressed activities of these metabolic enzymes when compared to tumor group, confirming that mitigating metabolic disorders contributed to the anti-tumor activities of ESAC. Moreover, ESAC could also reduced PD-L1 expression in tumor tissue, which was similar to the effect of PD-1 inhibitor. Furthermore, combination treatment with ESAC and PD-1 inhibitor was superior to either treatment alone, and it enhanced pyroptosis through the NLRP3/Caspase-1/GSDMD pathway. Together, we concluded that metabolic correction and PD-L1 inhibition were the critical mechanisms to exert anti-tumor activities of ESAC.
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