ANALYST
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2019
·Vol. 145
·期 5
·1801-1809
·
IF 4.019
Kaiqiang Wang,Da-Wen Sun,Hongbin Pu,Qingyi Wei
In this study, a 4-aminobenzenethiol-functionalized core–shell silver-coated gold nanoparticle (Au@Ag-4ABT NP) system was designed for the rapid sensing of carbendazim (CBZ) using a combination of naked-eye colorimetry and surface-enhanced Raman spectroscopy (SERS) dual-channel approach. Under alkaline conditions, the deprotonated CBZ species could interact with Ag surfaces via the N–Ag–O and N–Ag–N bonds. As a result, the neighboring Au@Ag-4ABT NPs would come closer through π–π interactions inducing the aggregation of Au@Ag-4ABT NPs. The aggregation of nanoparticles caused changes in the optical properties of the colloidal system, allowing observation with naked eyes, while the generation of more localized surface plasmon resonance “hotspots” between the adjacent Au@Ag nanoparticles permitted monitoring by the SERS technique. The proposed method showed effective sensitivity toward CBZ with limit of detection and limit of quantitation values of 37 and 122 ppb, respectively. In addition, the proposed dual-channel assay could serve as a simple and rapid method for sensing CBZ in tap water, cauliflower, and pear juice within 30 min.
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Analytical Methods
·
2024
·Vol. 16
·期 38
·6529-6537
·
IF 2.7
Na Li,Baokun Tang,Tao Zhu
Carbendazim (CBZ) is widely used for crop protection and its residues threaten human health and the environment. Therefore, developing an effective electrocatalyst is important for the extremely sensitive detection of CBZ. Lattice-strain engineering is an effective strategy to change its electronic structure and ultimately optimize the catalytic performance of materials, which can be used as a modification method to improve the detection performance of electrochemical sensors. Herein, a NiFeLDH@HsGY-NH2/MWCNTs heterojunction with strain effect is prepared by the electrostatic self-assembly method. The structure, morphology, composition, crystallinity and electrochemical performance of NiFeLDH@HsGY-NH2/MWCNTs are analyzed using various instrumental techniques, in which geometric phase analysis (GPA) and X-ray diffraction (XRD) images confirm the lattice-strain generated in NiFeLDH@HsGY-NH2/MWCNTs. The results indicate that the prepared electrochemical sensor exhibited an excellent response for carbendazim (CBZ) in the linear range of 0.05–50.00 μM with a detection limit of 10.00 nM (S/N = 3) under the optimal detection conditions. By analyzing the reasons for the improvement of the catalytic performance of the composite material, it is found that the composite of MWCNTs not only improves the conductivity of NiFeLDH but also regulates the electronic structure of metal atoms through double effects. This study provides new insights into the design of efficient and low-cost catalysts to facilitate electrochemical sensor applications.
研究领域:
材料科学
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环境科学
·
分析化学
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-12
·
IF 5.3
Xue Jinyan,Jia Yuzhen,Qi Linying,Yang Huaixia,Wang Yanzhi,Guo Liang
A novel aptamer-based sensor was developed using the signal amplification strategy of ring-opening metathesis polymerization (ROMP) and polyethyleneimine modified graphene oxide to achieve trace detection of carbendazim (CBZ). The dual identification of aptamer and antibody was used to avoid false positive results and improve the selectivity. Polyethyleneimine modified graphene oxide (GO-PEI), as a substrate material with excellent conductivity, was modified on the surface of a glassy carbon electrode (GCE) to increase the grafting amount of aptamer on the electrode surface. Moreover, a large number of cyclopentenyl ferrocene (CFc) was aggregated to form long polymer chains through ring-opening metathesis polymerization (ROMP), so as to significantly improve the detection sensitivity of the biosensor. The linear range of this sensor was 1 pg/mL–100 ng/mL with a detection limit as low as 7.80 fg/mL. The sensor exhibited excellent reproducibility and stability, and also achieved satisfactory results in actual sample detection. The design principle of such a sensor could provide innovative ideas for sensors in the detection of other types of targets. Graphical abstract
研究领域:
分析化学
·
电化学
·
生物传感器
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环境科学
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材料科学
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Kanru Fu,Zhengri Cong,Xinghao Wang,Haocheng Sun,Yong Zhang
Natural balsawood with quick-growing, light-weight and porous structure expects to be a carrier of self-supporting electrode for convenient pesticide residue analysis in food. In this work, the hydrangea-like Ni Ti layered double hydroxide (NiTi-LDH) is in-situ synthesized on the balsawood@few-layer Ti 3 C 2 T x nanosheets. The pyrolysis of such precursor leads to the carbonized balsawood@Ti 3 C 2 T x supported pompon-like Ni nanoparticles@N-doped carbon (CW@Ti 3 C 2 T x -NiNPs@NC), which demonstrates the two main structural advantages including the CW@Ti 3 C 2 T x with porous structure as the high-performance substrate and the NC immobilized the high-density distribution of NiNPs as the efficient electrocatalyst. These extraordinary characteristics of CW@Ti 3 C 2 T x -NiNPs@NC contribute to the outstanding electrochemical behaviors for detecting the low-concentration carbendazim (CBZ) pesticide, resulting in 0.36 nM of detection limit and the two-stage linear detection ranges including 1–250 nM and 0.25–20.00 μM. More importantly, such CW@Ti 3 C 2 T x -NiNPs@NC with free-cutting can be directly utilized as the quickly replaceable working electrode to create a prototype of portable sensor with the self-designed electronic system. Furthermore, a rapid sampling process matched such portable electrochemical sensor is proposed for the quick detection of residual CBZ on the surfaces of vegetables and fruits. Our research proposes a method to construct the high-performance self-supporting electrode on the basis of natural balsawood@Ti 3 C 2 T x nanosheets for electrochemical sensing.
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ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Yanyan Yan,Shasha Sun,Ning Zhao,Wanying Yang,Qinghua Shi,Biao Gong
Melatonin (Mel) serves as an important signalling molecule in various aspects of stress tolerance in plants. However, the function of Mel in pesticide metabolism remains unknown. Here, selecting the widely used fungicide carbendazim (MBC) as the model, we found that exogenous Mel had the ability to alleviate pesticide phytotoxicity and residues in tomato as well as in some other vegetables. Additionally, overexpression of the Mel biosynthetic gene caffeic acid O-methyltransferase 1 ( COMT1 ) significantly enhanced the capacity of the tomato to reduce MBC phytotoxicity and residue. This outcome was mainly because of the Mel-induced antioxidant capability, as well as the key detoxification process. Indeed, levels of reactive oxygen species (ROS) and lipid peroxides significantly decreased after applying exogenous Mel or overexpressing COMT1 , which resulted from direct ROS scavenging, and increased Mel levels significantly enhanced antioxidant enzymatic activity. More importantly, Mel activated the ascorbate-glutathione cycle to participate in glutathione S-transferase-mediated pesticide detoxification. A grafting experiment showed that rootstocks from COMT1 transgenic plants increased the Mel accumulation of wild-type scions, resulting in MBC metabolism in the scions. To our knowledge, this is the first report providing evidence of Mel-induced pesticide metabolism, which provides a novel approach for minimizing pesticide residues in crops by exploiting plant self-detoxification mechanisms.
研究领域:
植物生理学
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药理学
·
农业科学
·
生物化学
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INORGANIC CHEMISTRY
·
2025
·
IF 4.7
Linbo Deng,Shuya Xue,Li Li,Rongrong Huang,Pingying Xie,Jiawei Liu,Linyu Wang,Xiaoqiang Wang,Xuemin Duan,Limin Lu
Metal–organic framework (MOF)-derived carbon materials are commonly used as sensing materials, but their limited accessibility to active sites and specific surface area restrict their applications. In this study, we proposed a simple method involving facile tannic acid etching and calcination to create NiCo-embedded hollow N-doped carbon nanocubes (NiCo@HNC) using MOFs as sacrificial templates. The resulting NiCo@HNC material has a large specific surface area, improved mass transport channels, and enhanced exposure of electroactive sites due to its unique hollow structure and high porosity, leading to enhanced electrocatalytic activity. Moreover, with the utilization of NiCo@HNC as a substrate material and dopamine as a functional monomer, a ratiometric molecularly imprinted electrochemical sensor was developed for the selective and sensitive detection of carbendazim (CBZ). This sensor demonstrated a wide linear range of 0.0003–10 μM and a low detection limit of 0.1 nM. In addition, the sensor was utilized for the accurate quantification of CBZ in actual carrot/strawberry samples with a recovery range of 96.0–103.0%, indicating its potential for practical application.
研究领域:
材料科学
·
分析化学
·
环境科学
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2019
·
IF 6.361
Chengxi Zhu,Dong Liu,Zhi Chen,Libo Li,Tianyan You
We reported a carbon nanohorns/gold nanoparticles composites-based impedimetric aptasensor for carbendazim (CBZ) detection in lettuce and orange juice at picogram levels. The increased electron-transfer resistance, resulting from the formation of CBZ-aptamer complex, was recorded by electrochemical impedance spectroscopy as the aptasensor response for CBZ. Under the optimal conditions, the proposed aptasensor displayed a linear response for CBZ ranging from 1 to 1000 pg mL −1 with a detection limit of 0.5 pg mL −1 . Noteworthy, the as-developed aptasensor displayed the lowest detection limit for CBZ among the previously reported methods. Common pesticides (atrazine, thiamethoxam , etc.) with 100-fold concentration did not interfere the CBZ detection. For CBZ detection in lettuce and orange juice, satisfactory recoveries were obtained with standard addition method. Statistics demonstrated that no significant differences were found between the data provided by standard HPLC-MS reference method and developed aptasensing method in term of accuracy and precision. We believe that the proposed aptasensor possesses a potential application for CBZ monitoring in agricultural product and food.
研究领域:
分析化学
·
食品安全
·
纳米技术
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2025
·
IF 4.6
Dongjian Li,Mingxin Han,Liangzhuang Wang,Chengyun Si,Yingxiang Du,Xuefang Luo
Pesticide residues pose serious threats to food safety and human health, highlighting the need for rapid and sensitive detection methods. Traditional techniques are often time-consuming and complex, whereas Surface-Enhanced Raman Spectroscopy (SERS) offers a fast and highly sensitive alternative. In this study, silver nanoparticles (AgNPs) were synthesized via a one-pot hydrothermal method using trisodium citrate (TSC) as a reducing agent and polyvinylpyrrolidone (PVP) as a capping agent. By optimizing PVP and TSC concentrations, a novel AgNPs@capillary SERS substrate was fabricated through in situ assembly of AgNPs onto the inner walls of glass capillaries. The substrate enabled rapid, on-site detection of pesticide residues, specifically the neonicotinoid acetamiprid (ACE) and benzimidazole fungicide carbendazim (CBZ). SERS measurements showed strong linear correlations for ACE (R² = 0.9713, 0.01–0.5 μg/mL) and CBZ (R² = 0.9566, 0.005–0.5 μg/mL). Importantly, the detection limits reached 2.28 × 10⁻³ μg/mL for ACE and 6.25 × 10⁻³ μg/mL for CBZ, demonstrating excellent sensitivity. This study confirms the effectiveness of the one-pot synthesized AgNPs@capillary SERS platform as a practical tool for trace-level pesticide monitoring in environmental and food safety contexts.
研究领域:
食品安全
·
分析化学
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Runqiang Liu,Yuqi Chang,Fang Li,Volodymyr Dubovyk,Dongdong Li,Qiwen Ran,Hongyuan Zhao
We reported a simple and scalable strategy for the preparation of mung bean-derived porous carbon@chitosan (MBC@CTS) composite, which was used to optimize the glassy carbon electrode (GCE). The MBC@CTS/GCE sensor was applied for the carbendazim (CBZ) detection. For the MBC@CTS composite, MBC with three-dimensional hierarchical structure presented large specific surface area, good adsorbability, and high electrical conductivity, while CTS had good film-forming property, hydrophilicity performance, and adhesion capacity. The MBC@CTS/GCE sensor exhibited wonderful electrochemical detection performance towards CBZ. Under the optimized conditions, the MBC@CTS/GCE sensor showed a linear concentration range from 0.1 to 20 μM with relatively low limit of detection (LOD) of 20 nM. In addition, the fabricated sensor with good reproducibility, stability, and selectivity were successfully applied for the CBZ detection in apple and tomato juices with low relative standard deviation of 2.4 %–4.2% and satisfactory recoveries of 98.8–103.2%.
研究领域:
电分析化学
·
食品安全监测
·
纳米复合材料
·
环境与农业分析
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ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Jifan Chen,Xuhua Li,Xiangyu Li,Jing Wu,Xiaoli Han,Haihua Wang,Dong Yang
Background Electrochemical sensors offer high sensitivity for detecting analytes at ultralow concentrations (nM-pM) but have limited specificity towards target molecules. Nanomaterial modifications are employed to enhance conductivity and catalytic activity. Critically, molecularly imprinted polymers (MIP) provide a solution for specificity by creating selective recognition cavities complementary to the target analyte. Furthermore, dual-signal ratiometric strategies are indispensable to intrinsically correct for matrix effects and environmental fluctuations, ensuring reliable quantification. For the fungicide carbendazim (CBD), crucial for food safety, reliable on-site detection demands overcoming these combined limitations. We thus developed a ratiometric sensor integrating conductivity-enhanced fluorine-doped β-FeOOH nanorods (F-β-FeOOH NRs) with a CBD-specific MIP layer. Results A novel ratiometric sensor was fabricated by growing F-β-FeOOH NRs on carbon cloth (CC) via one-step hydrothermal synthesis. F-doping was strategically employed to facilitate the electron transfer and enhance the redox reaction of [Fe(CN) 6 ] 3-∕4- . A MIP layer was electropolymerized on F-β-FeOOH NRs/CC using CBD as the template and resorcinol/nicotinamide as dual monomers, thereby creating selective recognition sites. The ratiometric mode utilized the CBD oxidation peak (analyte-specific response) and the internal reference signal from [Fe(CN) 6 ] 3-∕4- (internal reference). This dual-signal strategy achieved a wide linear range from 1 to 1000 nM with a low detection limit of 25.6 nM. Real-sample analysis in fruits showed a strong correlation with high performance liquid chromatography (HPLC) results, with recoveries ranging between 98.4% and 105.9%, confirming its accuracy in complex matrices. Significance This work pioneers the integration of fluorine-doped β-FeOOH NRs with MIP-based ratiometric sensing, significantly enhancing sensitivity and anti-interference capability for pesticide monitoring. The sensor’s portability, low cost, and reliability in real samples highlight its practicality for on-site detection of CBD residues in agriculture and food safety. The design strategy paves the way for adapting this platform to detect other environmental contaminants using tailored MIP.
研究领域:
分析化学
·
环境监测
·
食品安全
·
电化学传感
·
纳米材料
·
分子印迹
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SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Zhiwei Bao,Dou Wang,Yao Zhao,Ting Luo,Guilin Yang,Yuanxiang Jin
Carbendazim (CBZ) and procymidone (PRO) are two widely used fungicides in agriculture. However, there are still gaps in knowledge regarding about the potential hazards of joint exposure to CBZ and PRO in animals. Here, 6-week-old ICR mice were exposed to CBZ, PRO and CBZ + PRO for 30 days, and metabolomics were performed to discover the mechanism by which the mixture enhanced the effects on lipid metabolism. Co-exposure to CBZ + PRO elevated the body weights, relative liver weights and relative epididymis fat weights, but not in the single exposure groups. Molecular docking analysis suggested that CBZ and PRO combined with peroxisome proliferator-activated receptor (PPARγ) at the same amino acid site as the agonist rosiglitazone. The RT-qPCR and WB results demonstrated that the levels of PPARγ were higher in the co-exposure group than in the single exposure groups. In addition, hundreds of differential metabolites were discovered by metabolomics and enriched in different pathways, such as pentose phosphate pathway and purine metabolism. A unique effect, a decrease in glucose-6-phosphate (G6P) that promoted more NADPH production, was observed in the CBZ + PRO group. These results demonstrated that exposure to CBZ + PRO caused more serious lipid metabolism disorder in the liver than exposure to a single fungicide, which could provide some new insight for the toxic effects after fungicides joint exposure.
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Ya Lu,Zhuanfang Bi,Guangyi Shang
Detection of trace carbendazim in agricultural products or environment is challenging but important for human health and environmental protection. Developing a sensitive and recyclable surface-enhanced Raman scattering (SERS) substrate is one of the effective methods to detect such pesticide residues. In this work, we developed a facile method to fabricate a three-dimensional cactus-like Ag nanoparticles/CuO/Cu2O nanocomposite (CACN) based on a galvanic replacement reaction between Tollens’ reagent and Cu foam. This design combined the Raman enhancement effect and photocatalytic activity, providing a multifunctional and reusable substrate for highly sensitive SERS detection. It was found that the enhancement and photocatalysis of the CACN substrate were closely related to the concentration of Tollens’ reagent, and the 10-CACN substrate (prepared at 10 mM) demonstrated excellent sensitivity, reproducibility, and recyclability. The substrate was successfully applied for the detection of carbendazim solution with a detection limit of 1.5 × 10–10 M and could be reused up to 7 times. More importantly, the substrate can be employed for the detection of trace carbendazim in real apple peels with a detection limit of ∼8.5 × 10–4 ppm, which is far below that of 2.0 × 10–1 ppm suggested by China, United Kingdom, and European Union. These results suggest that the facile fabrication method and the excellent substrate would facilitate the SERS technology closer to practical application in the detection of pesticide residues.
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