Nanomaterials
·
2022
·Vol. 12
·期 4
·691
·
IF 5.719
Huanyu Xie,Cairou Chen,Jiansen Lie,Ruiyun You,Wei Qian,Shan Lin,Yudong Lu
The use of clenbuterol (CLB) in large quantities in feedstuffs worldwide is illegal and potentially dangerous for human health. In this study, we directly prepared nitrogen-doped graphene quantum dots (N-GQDs) by a one-step method using cationic-etherified starch as raw material without pollution, which has the advantages of simple, green, and rapid synthesis of N-GQDs and high doping efficiency of nitrogen elements, compared with the traditional nitrogen doping method of reacting nitrogen source raw material with quantum dots. The N-GQDs synthesized by cationic etherification starch with different substitution degrees (DSs) exhibit good blue-green photoluminescence, good fluorescence stability, and water solubility. By comparing the fluorescence emission intensity of the two methods, the N-GQDs prepared by this method have higher fluorescence emission intensity and good fluorescence stability. Based on the static quenching mechanism between CLB and N-GQDs, a fluorescent probe was designed to detect CLB, which exhibited a wide linear range in the concentration range of 5 × 10−10~5 × 10−7M (R2= 0.9879) with a limit of detection (LOD) of 2.083 × 10−13M. More excitingly, the N-GQDs fluorescent probe exhibited a satisfactory high selectivity. Meanwhile, it can be used for the detection of CLB in chicken and beef, and good recoveries were obtained. In summary, the strategic approach in this paper has potential applications in the detection of risky substances in the field of food safety.Keywords:nitrogen-doped graphene quantum dots;fluorescence;cationic-etherified starch;fluorescence quenching;clenbuterol
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materiomics
·
2020
·
IF 5.797
Pramod K. Kalambate,Sharad S. Upadhyay,Yue Shen,Wanida Laiwattanapaisal,Yunhui Huang
A highly sensitive and selective electrochemical chiral sensor was developed based on the competitive supramolecular interaction of carbon nanofibers (CNFs) embedded sulfobutyl ether-β-cyclodextrin (SBE-β-CD) with optically active cationic drugs at glassy carbon electrode (GCE). The difference in intermolecular hydrogen bonding/stability constant/enantioselectivity coefficient and Gibbs free energy of anionic host SBE-β-CD with enantiomers of amlodipine (R-/S-AML), clenbuterol (R-/S-CBL) and metoprolol (R-/S-MET) as a guest paved the way for efficient discrimination. The proposed sensing platform (CNFs-SBE-β-CD/GCE) could recognize the aforementioned enantiomers based on the discernible difference of peak potential (S/R-AML (ΔE p = 135 mV), R/S-MET (ΔE p = 99 mV) and R/S-CBL (ΔE p = 111 mV). The binding mechanisms and thermodynamic study of the enantiospecific behavior have been investigated using the host-guest chemistry approach inside the nanocavity and results suggest that S-AML, R-MET, and R-CBL show stronger stability constants than their antipodes. Formation of the diastereomeric complex was taken as a measure of enantioselectivity and experimental results indicated that anionic SBE-β-CD is a better chiral ligand than neutral cyclodextrins. The fabricated sensor could be a useful low-cost electrochemical tool for molecular recognition of a variety of cationic species not only of drugs but also from other sources.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2013
·
IF 4.612
Hongyuan Yan,Suting Liu,Mengmeng Gao,Ning Sun
New ionic liquid modified dummy molecularly imprinted microspheres (DMIMs) were synthesized by aqueous suspension polymerization using phenylephrine as dummy template and 1-allyl-3-ethylimidazolium bromide as co-functional monomer. The obtained DMIMs were characterized by Fourier transform infrared (FT-IR) and scanning electron microscopy (SEM), and empolyed as the special sorbent of solid-phase extraction for isolation of clenbuterol (CLB) and clorprenaline (CLP) from urine sample. The results indicated that the ionic liquid modified polymers were monodispersed microspheres and had high selectivity and adsorbability to CLB and CLP, and the matrix interferences could be efficiently eliminated from the samples. Meanwhile, the effect of template leakage of DMIMs on quantitative analysis was avoided. Compared with other commercial sorbents (HLB, PCX, C 18 and SCX), the DMIMs exhibited higher affinity and purification ability to CLP and CLB. Under the optimized conditions, good linearity was observed in a range of 0.90–400 μg L −1 ( r 2 = 0.9999) with LOD of 0.19 and 0.070 μg L −1 for CLP and CLB, respectively. The recoveries at three spiked levels were ranged from 93.3% to 106% with RSD ≤ 5.6% ( n = 3).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Haiyun Zhai,Zhenping Liu,Zuanguang Chen,Zhixian Liang,Zihao Su,Shumei Wang
A novel electrochemical sensor was designed to determine clenbuterol by using a new hybrid nanomaterials of water-dispersible sulfonated graphene sheets (SGSs) and oxygen-functionalized multi-walled carbon nanotubes (MWCNTs-COOH) modified glass carbon electrode (GCE). The π–π stacking interaction between SGSs and MWCNTs-COOH was verified by UV–vis spectroscopy, fourier transform-infrared spectroscopy and scanning electron microscopy. The anodic peak current (Ipa) of clenbuterol at the GCE modified with SGSs/MWCNTs-COOH was much higher than those at the bare GCE, SGSs/GCE and MWCNTs-COOH/GCE due to the synergistic effect of the modified nanomaterials. Differential pulse voltammetry disclosed a good linear relationship between Ipa and concentrations of clenbuterol (0.01–5.0 μM), with the detection limit of 4.6 nM. Finally, the modified electrode was successfully used to determine clenbuterol in liver samples.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Kun Zhang,Yu Ge,Shaolang He,Fei Ge,Qianru Huang,Zhong Huang,Xinyue Wang,Yangping Wen,Binqiang Wang
Kudzu vine biochar (KVB) modified flexible carbon electrode (FCE) for the portable wireless intelligent sensing of clenbuterol (CLB) in bovine serum using cheap miniaturized analyzer composed of both smartphone and electrochemical mini-workstation was developed. Kudzu vine derived from abundant biomass residues of important traditional Chinese medicinal vine plants was selected for the preparation of KVB produced by pyrolyzing fine powders of kudzu vine at high temperature under oxygen-limited conditions. The morphology of KVB was characterized by scanning electron microscopy. FCE as flexible substrate electrode was prepared by the laser direct writing technique. The KVB modified FCE displayed excellent electrocatalytic response towards CLB in wide liner ranges from 0.95 to 14.31 μM with a low limit of detection of 0.75 μM. Practical application with satisfactory results will provide new opportunity for the comprehensive utilization of biomass residues of traditional Chinese medicinal plants as low-cost biochar for advanced electrode materials in portable wireless intelligent sensing platform based on cheap miniaturized analyzer composed of both smartphone and electrochemical miniworkstation for harmful residues in the quality safety of agro-products and food.
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Runxian Li,Decheng Suo,Zhengjie Yu,Zhiming Xiao,Peiling Wei,Shi Wang,Yuchao Feng,Xia Fan
The abuse of illegal β2-adrenergic agonists in animal feeding poses a threat to human health. Fluorescent lateral flow immunoassay (LFIA) is a promising point-of-care testing (POCT) method for monitoring illicit drug use on farms. Here, a traffic light- dual-color quantum dot nanobeads based fluorescent lateral flow immunoassay (QBs-LFIA) was developed for simultaneously detecting ractopamine (RAC) and clenbuterol (CLE). CdSe/ZnS quantum dot nanobeads that emit red and green fluorescence were introduced in this study. They were conjugated with anti-RAC and anti-CLE monoclonal antibodies, respectively, to prepare traffic light fluorescent probes (QBs-mAbs). The dual-color QBs-mAbs greatly enhanced the analytical performance and solved the restriction that multiple mAbs on the control line cannot be accurately distinguished in traditional single-color LFIA. Under optimal conditions, the limits of detection (LODs) for RAC and CLE were 15.7 and 36.3 pg/mL, respectively. The recoveries in spiked cattle urine ranged from 80.3 to 106.1 % with relative standard deviations (RSDs) less than 13.2 %. Good correlations were observed between the detection results of QBs-LFIA and LC-MS/MS (R 2 = 0.9931). The proposed traffic light QBs-LFIA provides a novel approach for simultaneously detecting RAC and CLE. It is also a promising POCT platform for rapid, sensitive, and on-site detection of multiple drug residues.
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·5011-5018
·
IF 3.978
Xinyang Zhang,Jianan Wen,Lili Lian,Xianhong Ma,Xiyue Wang,Dawei Lou
3D magnetic porous carbon (MPCK) was prepared using the metal–organic framework (MOF) MIL-100(Fe) as the carbon precursor by carbonisation and KOH activation strategies. Carbonisation and activation ensured that MPCK possessed excellent structural and thermal stability, strong magnetic responsiveness and high surface area. MPCK was used as an adsorbent for the magnetic solid-phase extraction of clenbuterol and ractopamine from mutton samples. The concentration levels of analytes were determined by ultra-high performance liquid chromatography-mass spectrometry. Under optimised extraction conditions, the peak area responded linearly to analytes over the concentration range from 0.05 μg L−1 to 40 μg L−1 (r ≥ 0.9972). The detection limits of clenbuterol and ractopamine were found to be 0.130 μg kg−1 and 0.150 μg kg−1, respectively. The satisfactory recoveries in mutton samples ranging from 95.64% to 114.65% indicated that 3D porous carbon is a promising adsorption material for the extraction of clenbuterol and ractopamine from complex biological matrixes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 3
·855-862
·
IF 3.719
Yan Fufeng,Zhang Yuanchang,Zhang Shuai,Zhao Jihong,Liu Shunli,He Linghao,Feng Xiaozhong,Zhang Hongzhong,Zhang Zhihong
We report on an efficient method for the production carboxy-modified graphene (carboxy-GR), and its application to a highly sensitive immunoassay for the livestock feed additive clenbuterol. Octadecanoic acid is shown to self-assemble on carboxy-GR via intermolecular interaction. Goat anti-mouse IgG was conjugated to 1-octadecanethiol-modified carboxy-GR and then deposited by self-assembly on a C18-modified gold surface. The resulting sensor surface was used to quantify clenbuterol by both surface plasmon resonance and electrochemical impedance spectroscopy. The assay works in the 0.01 ng∙mL −1 to 10 ng∙mL −1 concentration range and the lower detection limit is 6.57 pg∙mL −1 . It is concluded that this fairly simple method can be extended to immunoassays for other food additives.
Principle of sensor system and immunoassay. (i) Binding of ACG–IgG with the alkyl chain of 1-octadecanethiol through intermolecular force onto the gold substrate; (ii) absorbance of Ab CLB onto the surface of ACG-IgG; and (iii) detection of CLB using an immunoassay based on Ab CLB –ACG–IgG.
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Lihong Su,Huilan Hu,Yanli Tian,Conghui Jia,Lulu Wang,Han Zhang,Jianlong Wang,Daohong Zhang
Lateral flow immunoassay (LFIA) has emerged as an effective technique in the field of food safety and environmental monitoring. However, sensitive and quantitative detection is still challenging for LFIAs in complex environments. In this work, a dual-model colorimetric/SERS lateral flow immunoassay for ultrasensitive determination of clenbuterol was constructed based on a metallic core–shell Au/Au nanostar acting as a multifunction tag. Raman reporter molecules are located between the core (AuNP) and shell (Au nanostar) to form a sandwich structure, which contributes to eliminate the environmental interference and improve the detection stability. In addition, the Au/Au nanostar provides a much higher Raman enhancement due to the presence of sharp tips and larger surface roughness in comparison with gold nanoparticles (AuNPs). Thus, on the basis of the antibody–antigen interaction, the dual-model immunoassay can produce strong colorimetric and surface-enhanced Raman spectroscopy (SERS) signals for highly sensitive detection of the target analyte, clenbuterol. Under optimal conditions, clenbuterol could be detected by the colorimetric model with a visual detection limit of 5 ng/mL. Meanwhile, the SERS signal of the Au/Au nanostar was accumulated on the test line for the SERS model detection with a quantitative detection limit as low as 0.05 ng/mL, which is at least 200-fold lower than that of the traditional AuNPs-based immunoassay. Furthermore, recovery rates of the proposed method in food samples were 86–110%. This dual-model immunoassay provides an effective tool for antibiotic residues analysis and demonstrates a broad potential for future applications in food safety monitoring.
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Nuo Duan,Shuo Qi,Yucong Guo,Wei Xu,Shijia Wu,Zhouping Wang
Clenbuterol hydrochloride (CLB), a kind of β-agonist, has been illegally used as a feed additive for growth promotion in the food industry in many countries. Herein, surface-enhanced Raman scattering (SERS) combined with aptamer recognition was used for the ultrasensitive and quantitative detection of CLB. Fe 3 O 4 @ [email protected] nanoparticles displaying strong SERS enhancement were synthesized and used as the active substrate. Aptamer against CLB was immobilized on the surface of the substrate to form the capture probe. The complementary DNA (cDNA) was linked to gold nanoparticles (AuNPs) carrying the Raman reporter 4-mercaptobenzoic acid (4-MBA) to form the signal probe. The detection of CLB was carried out by competitive binding between free CLB and cDNA linked on the signal probe with the aptamer immobilized on the capture probe. The SERS intensity was significantly improved by the coupling effect between the Fe 3 O 4 @ [email protected] and AuNPs, resulting in high sensitivity for the quantification assays with a limit of detection of 0.003 ng mL −1 . The recoveries of the CLB from spiked pork samples were 90.7–108.0% and the coefficients of variation (CV%) were 2.2–3.1%, demonstrating the high precision and accuracy of the assay.
✓ 已验证
产品关联
DOI
高影响因子
LAB ON A CHIP
·
2024
·Vol. 24
·期 8
·2272-2279
·
IF 6.1
Huilan Hu,Jiaqi Tian,Rui Shu,Huihui Liu,Shaochi Wang,Xuechi Yin,Jianlong Wang,Daohong Zhang
A highly sensitive lateral flow immunoassay (LFIA) is developed for the enzyme-catalyzed and double-reading determination of clenbuterol (CLE), in which a new type of probe was adopted through the direct electrostatic adsorption of ultra-small copper–gold bimetallic enzyme mimics (USCGs) and monoclonal antibodies. In the assay, based on the peroxidase activity of USCG, the chromogenic substrate TMB–H2O2 was introduced to trigger its color development, and the results were compared with those before catalysis. The detection sensitivity after catalysis is 0.03 ng mL−1 under optimal circumstances, which is 6-fold better than that of the traditional Au NPs-based LFIA and 2-fold greater than that before catalysis. This approach was successfully applied to the detection of CLE in milk, pork and mutton samples with an optimum assay time of 7 min and best catalytic time of 80 s, after which satisfactory recoveries of 98.53–117.79% were obtained. Cu–Au nanoparticles as a signal tag and the use of their nanozyme properties are the first applications in the field of LFIA. This work can be a promising exhibition for the application of a cheaper substitute for HRP, ultra-small bimetallic enzyme mimics, in LFIAs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）