Susceptibility point-of-care testing (POCT) and bacterial inactivation are crucial for food safety and clinical diagnosis since bacteria pose a severe hazard to public health and healthcare. To enable photothermal determination and hyperthermal inactivation of bacteria, vancomycin-modified near-infrared (NIR)-responsive copper selenide nanoparticles (Cu 2−X [email protected] NPs) with excellent photothermal conversion are synthesized here. Cu 2−X [email protected] NPs can be trapped on the surface of Gram-positive bacteria such as Staphylococcus aureus ( S. aureus ), forming a Cu 2−X [email protected] NPs/ S. aureus complex. Suspension (Cu 2−X [email protected] NPs) and sediment (Cu 2−X [email protected] NPs/ S. aureus ) can convert the NIR laser energy into a thermal signal that can be detected by the thermal camera at concentrations as low as 6.0 CUF mL −1 . Meanwhile, S. aureus can be effectively inactivated by localized temperature rises during temperature-based signal detection. The antibacterial efficiency can reach as high as 99.53%. The proposed strategy not only provides a sensitive POCT strategy for bacterial determination but also implements efficient inactivation of bacteria simultaneously.
The treatment of wound inflammation is intricately linked to the concentration of reactive oxygen species (ROS) in the wound microenvironment. Among these ROS, H2O2 serves as a critical signaling molecule and second messenger, necessitating the urgent need for its rapid real-time quantitative detection, as well as effective clearance, in the pursuit of effective wound inflammation treatment. Here, we exploited a sophisticated 3D Cu2-xSe/GO nanostructure-based nanonzymatic H2O2 electrochemical sensor, which is further decorated with evenly distributed Pt nanoparticles (Pt NPs) through electrodeposition. The obtained Cu2-xSe/GO@Pt/SPCE sensing electrode possesses a remarkable increase in specific surface derived from the three-dimensional surface constructed by GO nanosheets. Moreover, the localized surface plasma effect of the Cu2-xSe nanospheres enhances the separation of photogenerated electron-hole pairs between the interface of the Cu2-xSe NPs and the Pt NPs. This innovation enables near-infrared light-enhanced catalysis, significantly reducing the detection limit of the Cu2-xSe/GO@Pt/SPCE sensing electrode for H2O2 (from 1.45 μM to 0.53μM) under NIR light. Furthermore, this biosensor electrode enables in-situ real-time monitoring of H2O2 released by cells. The NIR-enhanced Cu2-xSe/GO@Pt/SPCE sensing electrode provide a simple-yet-effective method to achieve a detection of ROS (H2O2、-OH) with high sensitivity and efficiency. This innovation promises to revolutionize the field of wound inflammation treatment by providing clinicians with a powerful tool for accurate and rapid assessment of ROS levels, ultimately leading to improved patient outcomes.
Xijian Liu,Qian Wang,Chun Li,Rujia Zou,Bo Li,Guosheng Song,Kaibing Xu,Yun Zheng,Junqing Hu
A low-toxic difunctional nanoplatform integrating both photothermal therapy and chemotherapy for killing cancer cells using Cu2−xSe@mSiO2–PEG core–shell nanoparticles is reported. Silica coating and further PEG modification improve the hydrophilicity and biocompatibility of copper selenide nanoparticles. As-prepared Cu2−xSe@mSiO2–PEG nanoparticles not only display strong near infrared (NIR) region absorption and good photothermal effect, but also exhibit excellent biocompatibility. The mesoporous silica shell is provided as the carrier for loading the anticancer drug, doxorubicin (DOX). Moreover, the release of DOX from Cu2−xSe@mSiO2–PEG core–shell nanoparticles can be triggered by pH and NIR light, resulting in a synergistic effect for killing cancer cells. Importantly, the combination of photothermal therapy and chemotherapy driven by NIR radiation with safe power density significantly improves the therapeutic efficacy, and demonstrates better therapeutic effects for cancer treatment than individual therapy.
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