JOURNAL OF NANOBIOTECHNOLOGY
·
2026
·Vol. 24
·期 1
·150
·
IF 12.6
Li Bei,Chen Zhengqiang,Sun Yong,Gou Maling,Luo Yuting,Zhang Wenli,Ye Xiwen,Zhao Yaqin,Feng Lei,You Zhen
The deep-seated locations and hypoxic microenvironments of tumors critically hinder conventional phototherapies. To overcome these limitations, we engineered a novel Au@Cu 2 − x Se nanoprobe via precise aspect ratio control of gold nanorods for NIR-II plasmonic resonance and site-specific growth of Cu 2 − x Se domains, resulting in the formation of a unique “lollipop” nanostructure. This design enables single-wavelength NIR-II laser-activated photothermal therapy and dynamic dual-modal therapy with intrinsic hypoxia tolerance: the nanoprobes achieve exceptional photothermal stability while simultaneously driving photothermally enhanced chemodynamic activity through accelerated Fenton-like catalytic cycles. Crucially, this strategy not only eradicates deep tumors but also activates systemic antitumor immunity. Combined with real-time photoacoustic imaging guidance, the platform establishes an oxygen-independent paradigm for treating hypoxic solid tumors. Graphical
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高影响因子
TALANTA
·
2025
·
IF 6.1
Liu-Feng Yu,Jie Zhou,Jing Cao,Bing-Huang Zhu,Yue Huang,Li-Xia Yan,Xiao-Dong Huang,Yi Zhang,Wei Gao
Bovine Mastitis (BM) adversely impacts the health of dairy cows and the quality of milk. Rapid and precise detection to miRNA-146a, a biomarker of BM for early diagnosis, is therefore crucial for the safety of dairy products. Herein, a high-efficiency method for the detection of miRNA-146a was developed, integrating photothermal Cu 2-x Se nanoparticles (CS NPs), strand displacement amplification (SDA) and lateral flow assay (LFA). The core innovation utilizes photothermal materials for dual functionality: (1) CS NPs as a photothermal nanomaterial enables rapid photocontrolled SDA (pSDA) under 808 nm laser irradiation, achieving target amplification within 20 min−2.3 times faster than conventional water-bathed SDA; and (2) Au coated Cu 2-x Se nanoparticles (CSA NPs) facilitates quantitative detection within a specifically designed photothermal LFA (pLFA). This pLFA measures temperature changes (ΔT) in the test zone via infrared thermal imaging to quantify the single-labeled amplicons. The integrated pSDA-pLFA method achieves an excellent balance of speed and sensitivity, featuring a detection limit of 28.6 fM and a wide linear range of 1.0 × 10 2 fM to 1.0 × 10 8 fM (R 2 = 0.9907). Validation using spiked milk samples demonstrated strong consistency with quantitative PCR (qPCR) results, confirming the establishment of a novel, rapid, and quantitative pSDA-pLFA platform for miR-146a detection. This approach holds significant promise for enhancing dairy industry bio-surveillance and ensuring food safety.
研究领域:
食品安全
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分子诊断
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纳米生物技术
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兽医学
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生物传感器与生物分析方法
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高影响因子
JOURNAL OF POWER SOURCES
·
2025
·
IF 7.9
Xue Guo,Jiahui Cao,Yang Liu,Kun Zhao,Hui Peng,Jianbo Xiong,Bitao Su,Xiaobo Zhang,Ming Zhong
The integration of heterostructure engineering with carbon materials exhibits significant advantages in enhancing ion transport efficiency and stability, buffering volume changes, and improving electrochemical lithium/sodium storage performance of transition metal selenide electrodes. In this study, we employ binary metal-organic frameworks as the precursors to successfully prepare rod-shaped composites assembled by carbon-coated bimetallic heterostructure nanoparticles (Cu 2-x Se/Bi 2 Se 3 @C) through a controlled selenization process at varying temperatures. The optimized Cu 2-x Se/Bi 2 Se 3 @C-600 composite demonstrate exceptional lithium storage capabilities, achieving high reversible capacities of 1102.3 mAh g −1 at 0.2 A g −1 after 100 cycles and 640.6 mAh g −1 at 1.0 A g −1 after 500 cycles. Ex-situ XRD analysis and theoretical calculations are utilized to elucidate the electrochemical reaction mechanisms and the benefits of the heterointerface in enhancing performance. The density functional theory calculation results indicate that the heterointerface in Cu 2-x Se/Bi 2 Se 3 reduces diffusion energy barrier of surface Li + ions and enhances electronic conductivity. The unique structure and composition advantages also grant the optimal composite superior sodium storage performance with high specific capacity (442.7 mAh g −1 at 0.2 A g −1 after 100 cycles), exceptional rate capability (297.3 mAh g −1 at 10.0 A g −1 ), and long-term cyclability (254.7 mAh g −1 at 5.0 A g −1 after 500 cycles).
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高影响因子
Advanced Healthcare Materials
·
2026
·
IF 9.6
Meichan Wu,Puze Li,Xin Xin,Cheng Song,Xiangliang Yang,Qingxia Xu,Yan Liu,Yong Shen,Guoping Wang,Jun Hu
The activation and infiltration of cytotoxic T lymphocytes (CTLs) are the key of tumor immunotherapy. However, the suppressive tumor immune microenvironment usually inhibits the function of CTLs. Tumor-associated macrophages (TAMs) play an important role in remodeling the tumor immune microenvironment. In this work, probiotic Escherichia coli Nissle 1917 (EcN) is used as a bioreactor to achieve intracellular synthesis of copper selenide nanoparticles (Cu 2-x Se NPs), giving Cu 2-x Se@EcN, which effectively repolarize macrophages from pro-tumor M2 phenotype to anti-tumor M1 phenotype mainly through activating MAPK signaling pathway. The synthesized Cu 2-x Se NPs exhibited excellent photothermal performance, inducing hyperthermia-sensitized Fenton-like reaction to catalyze the generation of hydroxyl radical from H 2 O 2 , then resulted in immunogenic death effect of tumor cells and the repolarization of M2 macrophages. Combined with surface-expressed pathogen-associated molecular patterns (PAMPs) of bacteria, Cu 2-x Se@EcN synergistically promoted DCs maturation and TAMs reprogramming, eventually promoting the infiltration and activation of T cells. Therefore, the bacteria-inorganic hybrid nanomaterials showed satisfied effect in inhibiting tumor growth and recurrence.
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高影响因子
CRYSTENGCOMM
·
2018
·Vol. 20
·期 28
·4020-4024
·
IF 3.304
Xin Huang,Yu Zou,Jian Hao,Jiang Jiang
Uniform ZnSe hollow nanospheres with face-centered cubic structure were synthesized via a post-synthetic chemical transformation reaction from solid Cu2−xSe nanocrystals, with the help of tributylphosphine at room temperature under an ambient atmosphere. The formation of ZnSe hollow structure was based on the synergetic effect of isotropic cation exchange from Cu2−xSe to ZnSe and concurrent selective etching of Cu2−xSe templates through anion extraction. The degree of hollowness could be adjusted by changing the feed molar ratio of Zn/Cu and reaction temperature which determined the extent of cation exchange. These ZnSe hollow nanospheres exhibited excellent catalytic activity and stability for the photodegradation of dyes.
研究领域:
材料科学
·
化学工程
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2025
·
IF 2.5
Yang Yang,Hua Xie,Bi Zhou,Yuling Liu,Jiqing Hao
A single drug therapeutic approach fails to sufficiently eliminate tumors in clinics, owing to the tumoral complexity and heterogeneity. The integration of chemotherapy with novel treatment modalities offers a promising synergistic strategy for enhancing therapeutic outcomes. Herein, we successfully develop pH-triggered dynamic organic nanocomposite hydrogels for enhanced local tumor combination therapy, which are prepared through incorporation of Cu2-xSe nanoparticles (NPs) and lipophilic mixture (OPC) including orthoester compound, polycaprolactone and carboplatin into imine-crosslinked polysaccharide-based hydrogels. The dynamic imine linkages endow the hydrogels with favorable injectability and self-adaptability as well as tumoral extracellular pH-triggered degradation. OPC can enhance drug escape from hydrogels, tumor penetration and pH-responsive prolonged and sustained release of the drug. Cu2-xSe NPs endow the hydrogels with photothermal therapy (PTT), photodynamic therapy (PDT) and chemo-dynamic therapy (CDT). These superior physicochemical properties result in significant tumor growth inhibition while decreasing side effect on normal tissues. Therefore, the pH-responsive dynamic organic nanocomposite hydrogels have great potential for local tumor therapy in clinics.
研究领域:
肿瘤学
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生物材料
·
纳米医学
·
药物递送
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转化医学
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光热治疗
·
化学动力学治疗
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PubMed
高影响因子
Biomedical Materials
·
2023
·Vol. 18
·期 3
·035013
·
IF 4
Yali Liu,Wuyi Liu,Guobing Li,Wenjing Lai,Changpeng Hu,Qian Zhang,Min Zhou,Laichun Lu,Jingbin Huang
Cu2−xSe nanoparticles (Cu2−xSe NPs) as a new therapeutic drug platform is widely used in disease treatment due to their strong near-infrared optical absorption. In recent years, with their continuous expansion of applications in different fields, their own biological effects have received increasing attention. However, little is known about the effect of Cu2−xSe NPs on cancer cell. In this research, we found that Cu2−xSe NPs inhibited proliferation of HepG2 cells (IC50: 15.91 μM) and SMMC-7721 cells (IC50: 43.15 μM) and they mainly induced cell cycle arrest at the G2/M phase. Moreover, Cu2−xSe NPs inhibited HepG2 and SMMC-7721 cell migration and lamellopodia formation. Further studies indicated that Cu2−xSe NPs impaired mitochondrial respiration by inhibiting electron transport chain complex activity, thus reducing adenosine triphosphate levels. The insufficient energy supply subsequently impaired actin cytoskeleton assembly, ultimately inhibiting HepG2 and SMMC-7721 cell proliferation and migration. These findings suggest that Cu2−xSe NPs may have potentially antitumor activity, which might provide new insights of NPs into specific cancer treatment.
研究领域:
纳米技术
·
肿瘤学
·
细胞生物学
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药理学
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高影响因子
ACS Nano
·
2023
·
IF 17.1
Ke Li,Kun Xu,Ye He,Yulu Yang,Meijun Tan,Yulan Mao,Yanan Zou,Qian Feng,Zhong Luo,Kaiyong Cai
The hypoxia microenvironment of solid tumors poses a technological bottleneck for ferroptosis and immunotherapy in clinical oncology. Nanoreactors based on special physiological signals in tumor cells are able to avoid various tumor tolerance mechanisms by alleviating the intracellular hypoxia environment. Herein we reported a nanoreactor Cu2–xSe that enabled the conversion of Cu elements between Cu+ and Cu2+ for the generation of O2 and the consumption of intracellular GSH content. Furthermore, to enhance the catalytic and ferroptosis-inducing activities of the nanoreactors, the ferroptosis agonist Erastin was loaded on the ZIF-8 coating on the surface of Cu2–xSe to up-regulate the expression of NOX4 protein, increase the intracellular H2O2 content, catalyze the Cu+ to produce O2 and activate ferroptosis. In addition, the nanoreactors were simultaneously surface functionalized with PEG polymer and folic acid molecules, which ensured the in vivo blood circulation and tumor-specific uptake. In vitro and in vivo experiments demonstrated that the functionalized self-supplying nanoreactors can amplify the ability to generate O2 and consume intracellular GSH via the interconversion of Cu elements Cu+ and Cu2+, and impair the GPX4/GSH pathway and HIF-1α protein expression. At the same time, by alleviating the intracellular hypoxia environment, the expression of miR301, a gene in the secreted exosomes was decreased, which ultimately affected the phenotype polarization of TAMs and increased the content of IFN γ secreted by CD8+ T cells, which further promoted the ferroptosis induced by Erastin-loaded nanoreactors. This combined therapeutic strategy of activating the tumor immune response and ferroptosis via self-supplying nanoreactors provides a potential strategy for clinical application.
研究领域:
肿瘤学
·
纳米技术
·
免疫治疗
·
癌症生物学
·
分子生物学
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高影响因子
ACS Nano
·
2024
·
IF 15.8
Hao Zhang,Li Yang,Mengxiao Han,Yaobao Han,Zhilin Jiang,Qing Zheng,Jun Dong,Tingting Wang,Zhen Li
The currently available immune checkpoint therapy shows a disappointing therapeutic efficacy for glioblastoma multiforme (GBM), and it is of great importance to discover better immune checkpoints and develop innovative targeting strategies. The discovered metabolic immune checkpoint ecto-5-nucleotidase (CD73) in a tumor contributes to its immune evasion due to the dysregulation of extracellular adenosine (ADO), which significantly inhibits the function of antitumor T cells and increases the activity of immunosuppressive cells. Herein, we drastically inhibit the expression of CD73 to reduce the production of ADO by using versatile Au@Cu2–xSe nanoparticles (ACS NPs). ACS NPs can decrease the expression of CD73 by alleviating the tumor hypoxia through their Fenton-like reaction to weaken the ADO-driven immunosuppression for enhancing antitumor T cell infiltration and activity of GBM. The copper ions (Cu2+) released from ACS NPs can chelate with disulfide, leading to the formation of cytotoxic bis(N,N-diethyldithiocarbamate)-copper complex (CuET), which can be combined with radiotherapy to recruit more antitumor T cells to infiltrate into the tumor site. Based on the inhibition of CD73 to promote the infiltration and activity of antitumor T cells, a cascade of enhancing GBM immunotherapy effects can be achieved. The significant increase in CD8+ T and CD4+ T cells within the tumor and the memory T cells in the spleen effectively reduces tumor size by 92%, which demonstrates the excellent efficacy of immunotherapy achieved by a combination of metabolic immune checkpoint CD73 inhibition with chemoradiotherapy. This work demonstrates that modulation of CD73-mediated tumor immunosuppression is an important strategy of improving the outcome of GBM immunotherapy.
研究领域:
肿瘤学
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免疫治疗
·
纳米技术
·
癌症研究
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高影响因子
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