ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2023
·Vol. 30
·期 33
·80154-80161
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IF 5.8
Wang Qiutang,Xu Yanwen,Du Wenchao,Yin Ying,Wu Xuan,Sun Feifei,Ji Rong,Guo Hongyan
Understanding the distribution of di(2-ethylhexyl) phthalate (DEHP) is necessary for future risk evaluation of DEHP in agricultural soils. This study used 14 C-labeled DEHP to examine its volatilization, mineralization, extractable residues, and non-extractable residues (NERs) incubated in Chinese typical red and black soil with/without Brassica chinensis L. Results showed that after incubated for 60 days, 46.3% and 95.4% of DEHP were mineralized or transformed into NERs in red and black soil, respectively. The distribution of DEHP in humic substances as NER descended in order: humin > fulvic acids > humic acids. DEHP in black soil was more bioavailable, with 6.8% of initial applied radioactivity left as extractable residues at the end of incubation when compared with red soil (54.5%). Planting restrained the mineralization of DEHP by 18.5% and promoted the extractable residues of DEHP by 1.5% for black soil, but no such restrain was observed in red soil. These findings provide valuable information for understanding the distribution of DEHP in different soils and develop the understanding for the risk assessments of PAEs in typical soils. Graphical abstract
研究领域:
环境科学
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土壤学
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yi Zhao,Zi-Yan Hu,Ming Lou,Fu-Wei Jiang,Yi-Feng Huang,Ming-Shan Chen,Jia-Xin Wang,Shuo Liu,Yu-Sheng Shi,Hong-Mei Zhu,Jin-Long Li
Di-2-ethylhexyl phthalate (DEHP) is frequently used as a plasticizer to enhance the plasticity and durability of agricultural products, which pose adverse effects to human health and the environment. Aquaporin 1 (AQP1) is a main water transport channel protein and is involved in the maintenance of intestinal integrity. However, the impact of DEHP exposure on gut health and its potential mechanisms remain elusive. Here, we determined that DEHP exposure induced a compromised duodenum structure, which was concomitant with mitochondrial structural injury of epithelial cells. Importantly, DEHP exposure caused duodenum inflammatory epithelial cell damage and strong inflammatory response accompanied by activating the TLR4/MyD88/NF-κB signaling pathway. Mechanistically, DEHP exposure directly inhibits the expression of AQP1 and thus leads to an inflammatory response, ultimately disrupting duodenum integrity and barrier function. Collectively, our findings uncover the role of AQP1 in phthalate-induced intestinal disorders, and AQP1 could be a promising therapeutic approach for treating patients with intestinal disorders or inflammatory diseases.
研究领域:
毒理学
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胃肠病学
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分子生物学
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2020
·
IF 6.551
Nai-Xian Feng,Qi-Feng Liang,Yu-Xi Feng,Lei Xiang,Hai-Ming Zhao,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo,Ming-Hung Wong
Phthalate acid esters (PAEs) are ubiquitous pollutants in agricultural soils . Application of bioorganic fertilizer (BOF) containing beneficial microbes represents a promising approach to improve the yield and quality of crops grown in contaminated soils. In the present study, a novel multifunctional bioorganic fertilizer N-BOF was developed by using compost of sewage sludge and agricultural waste and inoculating with PAEs-degrading B. megaterium YJB3 and phosphate solubilizing B. megaterium YLYP1. Its feasibility of improving the yield and quality of vegetable grown in PAEs (including DBP and DEHP) contaminated soil was evaluated by pot experiments. The N-BOF could effectively promote plant growth, with biomass increasing by 4–66.9% and 19–110% compared to chemical (CF) and no fertilizer (CK), respectively. The concentrations of DBP and DEHP in shoots of chemically fertilised vegetable ranged 1.23–3.12 mg/kg (dry weight, DW) and 1.63–3.89 mg/kg (DW), respectively. Their concentrations were significantly decreased ( p < 0.05) when N-BOF was applied (1%, 2%, 5% amendment), especially at higher application rate ranging 0.11–0.3 mg/kg (DW) and 0.16–0.32 mg/kg (DW), respectively. Meanwhile, vegetable quality attributes were also significantly improved when 2% N-BOF was applied, with increase in the contents of vitamin C , vitamin B1, total protein, and starch, and decrease in the contents of nitrite and nitrate. In this case, the human health risk from consumption of the vegetable grown in PAEs-contaminated soil could be significantly reduced. Thus, our study is expected to provide an efficient way of high-value utilization of organic substrates by producing low-cost but high quality N-BOF. Future studies on the effects of N-BOF in terms of fertilizer regimes on yield and quality of the vegetable are needed, and further field studies for assessing the long-term efficacy and reliability of this promising N-BOF are also warranted.
研究领域:
环境科学
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农业科学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 3
·1-10
·
IF 5.7
Liu Yuyao,Deng Yuan,Liao Dongyun,Han Yujie,Wang Qiang,Guo Yujing,Bai Hongcun,Dong Chuan,Fan Lifang
A label-free and efficient electrochemical (EC) sensing platform for di-2-ethylhexyl phthalate (DEHP) was developed based on in situ probe nickel hexacyanoferrate nanoparticle (NiHCF NP)-decorated three-dimensional reduced graphene oxide (3D rGO) composites. NiHCF NPs in the composites as an in situ probe show a pair of well-defined peaks with good reversibility and stability. Coupling 3D rGO with NiHCF NPs not only improved the electron transfer capability of NiHCF NPs but also provided more sites for aptamer immobilization. The synthesized NiHCF NP-decorated 3D rGO composites were used to act as a substrate for the immobilization of anti-DEHP aptamer by the covalent bonding method. The designed EC sensing platform displays excellent sensing performance for DEHP with a low detection limit of 3.64 pg/L, and a linear working range of 0.01 − 1000 ng/L. The application of the sensing platform to actual environmental samples was studied and satisfactory results were obtained. Thus, the proposed EC sensing platform would provide a potential tool for efficient detection of pollutants in the environment. Graphical abstract
研究领域:
环境科学
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分析化学
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纳米技术
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电化学
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PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2026
·
IF 6.2
Jia-Gen Cui,Hao Zhang,Ming-Shan Chen,Jia-Xin Wang,Xue-Nan Li,Jin-Long Li
Lycopene (LYC), a natural antioxidant compound abundant in tomatoes, has established neuroprotective roles due to its antioxidant and anti-inflammatory properties. Di (2-ethylhexyl) phthalate (DEHP), a common plasticizer, is known to exert neurotoxic effects. However, the mechanisms of DEHP-induced neurotoxicity remain unclear. Furthermore, effective preventive strategies against this neurotoxicity are still lacking. This study, employing in vitro (BV2 cells) and in vivo (ICR mouse) models, demonstrates that LYC prevents DEHP-induced cerebellar damage by suppressing the triggering of dose-dependent microglial ferroptosis and neuroinflammation. Mechanistically, LYC inhibited the phosphatidylethanolamine-binding protein 1 (PEBP1)/arachidonate 15-lipoxygenase (ALOX15) axis (supported by immunofluorescence and Western blot assays), leading to reduced reactive oxygen species by approximately 48% in the cerebellum, which ultimately prevented neuroinflammation. Our findings reveal that LYC prevents DEHP-induced neurotoxicity by inhibiting microglial ferroptosis via the PEBP1/ALOX15 axis. Furthermore, our findings highlight the clinical promise of LYC as a novel neuroprotective treatment against environmental toxicant-induced damage.
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PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2025
·
IF 6.1
Guanglong Chen,Min Zeng,Wanjing Liu,Hongping Liao,Jun Wang
Micro- and nanoplastics (MNPs) and di(2-ethylhexyl) phthalate (DEHP) are ubiquitous environmental contaminants; however, the risks of their combined exposure to aquatic reproductive health, particularly sex-specific differences, remain incompletely understood. This study investigated the effects of single and combined exposure to polystyrene nanoplastics (PS-NPs; 0.1 and 1 mg/L) and DEHP (10 μg/L) on the reproductive endocrine system of adult male and female zebrafish ( Danio rerio ) Results demonstrated that PS-NPs accumulated in the zebrafish intestine. High concentrations of PS-NP and DEHP and their combined exposures induced significant adverse effects in both sexes, including gonadal tissue damage and oxidative stress. A striking sexual dimorphism was observed in endocrine responses. Females exhibited a pronounced suppression of both 17β-estradiol (E2) and testosterone (T) levels, which correlated with inhibited ovarian cyp19a gene expression under co-exposure. Conversely, male E2 levels remained stable and T levels decreased only under co-exposure, increasing E2/T ratio. This distinct male endocrine profile was associated with differential regulation of testicular cyp19a and 17β-hsd expression. This study confirms that PS-NPs and DEHP exert profound sex-specific reproductive toxicity in zebrafish. These effects are mediated by disrupting the hypothalamic-pituitary-gonadal (HPG) axis, while inducing sex-specific alterations in brain and gonadal gene expression. Overall, this study highlights the critical importance of considering sex differences and pollutant mixture effects in environmental risk assessments.
研究领域:
环境毒理学
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水生生态学
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内分泌学
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生殖生物学
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生态毒理学
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DOI
PubMed
高影响因子
PETROLEUM SCIENCE AND TECHNOLOGY
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2023
·
IF 1.5
Jue Wang,Heng Zhang,Wei Wu,Zeyun Wang
Use of the synergistic effect between nanomaterials and ionic liquids is of great significance for designing high-performance lubricating materials. In this work, Zn-MOF/[P88814]DEHP (zinc complexes with 2-aminoterephthalic acid nanosheets/trioctyltetradecylphosphonium bis (2-ethylhexyl) phosphate) composites were successfully synthesized by grinding the mixture of [P88814]DEHP and Zn-MOF with different mass ratios and evaluated as a new kind of the white oil additive using a four-ball testing machine. Results demonstrate that the optimal mass ratio of Zn-MOF to [P88814]DEHP was 1:1 and the Zn-MOF/[P88814]DEHP composite with 2.0 wt% addition amount into the white oil significantly reduced friction coefficient and wear. Such obvious friction-reducing and outstanding anti-wear properties of Zn-MOF/[P88814]DEHP composite could be attributed to the synergistic lubrication between Zn-MOF and [P88814]DEHP. This work investigated the synergistic lubrication between MOFs and ILs, which provided a new idea for designing and preparing new lubricating oil additives.
研究领域:
材料科学
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润滑工程
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高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2023
·
IF 6.1
Ming-Shan Chen,Jia-Xin Wang,Hao Zhang,Jia-Gen Cui,Yi Zhao,Jin-Long Li
Phthalates are widely used to improve the flexibility of poly(vinyl chloride) (PVC) polymer agriculture products. Di(2-ethylhexyl) phthalate (DEHP) is a type of addition to plastic and can lead to many health problems. Hemeoxygenase-1 (HO-1) is an extremely important molecule that releases enzymatic products to promote ferroptosis. This research aimed to explore the function of HO-1 in DEHP-induced renal proximal tubule cell ferroptosis. In the experiment, ICR male mice are exposed to (0, 50, 200, and 500 mg/kg BW/day) DEHP for 28 days. Here, we observed that DEHP induced glomeruli atrophy and the tubules swell. Furthermore, DEHP exposure could increase ferrous iron content and decrease antioxidant activity. We also found that DEHP exposure increased the expression of nuclear factor-erythroid 2 p45-related factor 2 (NFE2L2) in the nucleus. In particular, the expression of (HO-1) is significantly increased both in protein and mRNA levels. Glutathione peroxidase 4 (GPX4) as an endogenous control of ferroptosis was downregulated, which proved the occurrence of ferroptosis. In the study, exposure to DEHP activated the NFE2L2/HO-1 signaling pathway and resulted in ferroptosis of the proximal tubule. This research connects ferroptosis with HO-1, providing new insights into the potential roles of phthalates in nephrotoxicity.
研究领域:
毒理学
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肾脏生理学
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分子生物学
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DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Zhuozhi Gong,Qiujian Feng,Wenyu Chen,Cheng Xu,Siyuan Tang,Shengjing Liu
Objective To evaluate the association between di(2-ethylhexyl) phthalate (DEHP) exposure and testosterone deficiency in adult men, and to explore molecular changes and candidate compounds related to DEHP-associated male reproductive toxicity. Methods We used a multi-level integrative strategy. Computational toxicology predicted DEHP endocrine-disrupting potential. In NHANES 2013–2016 adult males, survey-weighted logistic regression and restricted cubic splines assessed associations between urinary DEHP metabolites and testosterone deficiency. Male Sprague-Dawley rats were exposed to DEHP to evaluate sex hormones, testicular/epididymal histopathology, oxidative stress, inflammation, and CD68⁺ macrophage infiltration. Integrated infertility transcriptomes with ensemble machine learning identified hub genes, which were mapped in testicular single-cell RNA-seq with virtual knockout–based network inference. Connectivity Map screening plus molecular docking and molecular dynamics prioritized candidate compounds. Results In NHANES, oxidative DEHP metabolites (MEHHP, MEOHP, and MECPP) were associated with increased odds of testosterone deficiency and showed monotonic dose-response increases. Rat experiments showed that DEHP reduced testosterone accompanied by decreased FSH/LH levels, disrupted testicular structure and spermatogenesis, and increased oxidative stress, inflammatory markers, and macrophage infiltration. Hub genes ( NNT, ACSS1, NANOG, S100A16, and CELF4 ) were mainly involved in mitochondrial energy metabolism and redox homeostasis. CMap screening and in silico analyses prioritized ursolic acid as a candidate compound, with the predicted ursolic acid–ACSS1 complex showing relative stability in molecular dynamics simulation. Conclusion DEHP exposure was associated with testosterone deficiency in men and induced reproductive toxicity in rats. Integrated analyses highlighted candidate metabolic, redox, inflammatory, and Sertoli cell-related network alterations. Ursolic acid was computationally prioritized as a candidate compound, but experimental validation is required.
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DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
QiMeng He,Hui Jin,Jie Shen
Di (2-ethylhexyl) phthalate (DEHP) is a widely used plasticizer, and many organisms encounter varying degrees of exposure in their natural environment. This study aimed to assess the impact of DEHP on Drosophila larvae by analyzing their behavior through kinematic parameters. We exposed the larvae to different concentrations of DEHP (0, 0.01%, 0.1%, 0.5%, 1% (v/v)), and their locomotor activity was observed, recorded, and the kinematic parameters were evaluated by machine learning method. The results indicated that the instantaneous velocity of larvae exposed to DEHP concentrations of 0.1%, 0.5%, and 1% significantly decreased, particularly in the 1% concentration group, where the instantaneous velocity dropped by 20.3%. Regarding instantaneous angular velocity, lower concentrations of DEHP resulted in an increase in instantaneous angular velocity; however, at a concentration of 1%, the instantaneous angular velocity significantly decreased by 22% compared to the control group. The results contribute to our understanding of the potential ecological and health risks posed to Insects and other organisms exposed to DEHP.
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高影响因子
Journal of Hazardous Materials Advances
·
2022
·
IF 0
Zhuo Chen,Qiwei Chen,Ying Xu,Jinhan Mo
PAEs usually adsorb on indoor surfaces to form organic films, which could be more hazardous after enriching VOCs. Two typical PAEs, DnBP and DEHP, in the film phase, were chosen to explore the enrichment capacity of gaseous toluene, hexanol, and benzyl alcohol in this study. Exposed to gaseous VOCs less than 250 ppb, film-phase DnBP and DEHP could capture benzyl alcohol significantly and hexanol slightly from the air, respectively, while toluene was hardly absorbed in the films. Henry's law constants and partition coefficients ( K ) of VOCs in DnBP or DEHP films were derived, supposing films as dilute solution systems. The K value of VOC in DnBP or DEHP films was 1∼2 orders larger than the octanol-air partition coefficient ( K oa ), indicating that the surface film composition greatly influences VOC adsorption. Benzyl alcohol was the largest VOC component in surface films, agreeing well with the molecular dynamics simulation results. The hydroxyl and phenyl groups of benzyl alcohol molecules may be key factors in VOC enrichment. They form hydrogen bonds with the ester group and π-π stacking interaction with the phenyl group of DnBP or DEHP molecules, respectively. After enriching VOCs, film-phase PAEs could evoke combined exposure risks to human beings.
研究领域:
环境科学
·
化学
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DOI
CHEMICAL ENGINEERING JOURNAL
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2020
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IF 10.652
Di Wang,Yuqing Sun,Daniel C.W. Tsang,Deyi Hou,Eakalak Khan,Daniel S. Alessi,Yaoyu Zhao,Jianyu Gong,Linling Wang
The high content and low removal efficiency of suspended solids (SS) in wastewater derived from shale gas development via hydraulic fracturing has raised advanced treatment problems ( e.g., organics removal) and environmental concerns. In this study, two kinds of suspended solids were separated from synthetic flowback wastewater (FWW) after 1 or 14 days of sedimentation. The physicochemical properties , porous structures, surface chemistry, and particle stability of the obtained 1-/14-day solids were characterized. To illustrate the specific roles of SS during radical-based FWW treatment , the removal of di-(2-ethylhexyl) phthalate (DEHP, a typical recalcitrant organic that has been identified in FWW) by a ferrous iron/peroxysulphate (Fe 2+ /PS) system was examined as a representative case. Results showed that DEHP was simultaneously adsorbed onto the solids and degraded by Fe 2+ /PS. The removal processes were governed by the phase distribution of DEHP and Fe 2+ , and the distribution was influenced by interactions between the 1-/14-day solids, Fe 2+ /PS, and various FWW components. Polyacrylamide (PAM), an anionic surfactant commonly detected in FWW, can attach to the solids and aid in maintaining the structural and compositional integrity of the solids. Due to stronger adsorption with PAM, the 14-day solids retained less DEHP and Fe 2+ , contributing to enhanced DEHP degradation in the solution phase as compared to the 1-day solids. Thus, sufficient sedimentation is advantageous for the degradation of recalcitrant organic contaminants ( e.g., DEHP) in FWW treatment. Moreover, certain FWW components ( i.e. , PAM and ethylene glycol) can interact with FWW solids, creating a positive synergistic effect on organic removal efficiency.
研究领域:
环境科学
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污水处理
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高影响因子
危险品化学品经营许可证（带存储）