JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Xiaohai Yan,Jia Li,Fudai Cai,Xuejun Zeng,Sijia Li,Kai Wang,Qinglin Sheng,Kewei Feng,Yahong Yuan,Tianli Yue
Patulin (PAT) is a mycotoxin with immunotoxicity, genotoxicity, and neurotoxicity, so effective methods are needed for its detection. Fluorescence aptasensors provide a homogeneous detection method, but their sensitivity needs to be further improved. Herein, we developed a fluorescence aptasensor based on a target-oligonucleotide-driven DNAzyme motor and defective Zr-TCPE for PAT detection. The defective Zr-TCPE achieved an enhanced fluorescence intensity (increased by ∼1.9-fold) via defect engineering. The target-oligonucleotide-driven DNAzyme motor achieved effective PAT transduction, with a signal background ratio 4.4-fold that of the competitive transduction strategy. By combining defective Zr-TCPE with a target-oligonucleotide-driven DNAzyme motor, we achieved ultrasensitive detection of PAT with a detection limit as low as 9.38 fg/mL. In addition, the proposed fluorescence aptasensor could be used for the detection of PAT in apple juice, with recoveries ranging from 95.93% to 105.71%. This fluorescence aptasensor provides reasonable guidance for trace detection of hazardous substances in food and the environment.
研究领域:
分析化学
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生物传感器
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食品安全
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纳米技术
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Xuejun Cui,Rongguo Li,Xiaofei Liu,Jingfeng Wang,Xueqi Leng,Xiaolei Song,Qianqian Pei,Yu Wang,Su Liu,Jiadong Huang
Herein, we have reported the development of a simple, rapid, and low cost colorimetric method for the detection of antibiotic based on target-activated split peroxidase DNAzyme coupled with dual nicking enzyme signal amplification (NESA). To lower background signal in G-quadruplex DNAzyme-based detection, the two split G-rich parts are caged into two different hairpin probes, respectively, preventing the two parts from assembling into the G-quadruplex structure. By the combination of restriction endonuclease-assisted cleavage reaction with the spilt G-quadruplex probes, target-modulated release of the two split G-rich parts is achieved, affording high specificity of antibiotic detection. Our strategy features with several aspects. First, the less background signal produced by the self-assembly of G-quadruplex in the absence of target is effectively eliminated owing to the pre-blocking of the two split G-rich parts. Second, dual NESA coupled G-quadruplex DNAzyme amplification strategy is integrated with colorimetric assay of antibiotic, which significantly improves the detection sensitivity. Third, peroxidase-mimicking DNAzyme is used as biocatalyst in our reaction system, which can catalyze the oxidation of 2,2' - azino - bis (3 - ethylbenzothiozoline - 6 - sulfonic acid) (ABTS 2− ) mediated by H 2 O 2 to generate the colored radical anion (ABTS •- ), allowing to low cost and visual detection of antibiotic by the naked eye. Under optimized conditions, the results revealed the proposed biosensor exhibits excellent specificity and sensitivity toward kanamycin with a detection limit as low as 14.7 pM. Hence, the target-activated split G-quadruplex DNAzyme and dual NESA-based strategy provides a useful and practical platform for antibiotic residues determination and other analytes detection in bio-analysis.
研究领域:
生物化学
·
分析化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
Materials Express
·
2023
·Vol. 13
·期 11
·1915-1921
·
IF 0.7
Chen Sihan,Hu Huiqi,Jiang Wenting,Zhang Yuyun,Luo Xiaohu,Peng Jiayi,Li Le,Zhen Deshuai,Wang Hanqing
A novel near-infrared induced upconversion sensor for the detection of uranyl ions was successfully constructed using β-NaYF4: Yb, Tm, Gd upconversion nanoparticles (UCNPs) and specific DNAzyme. Characterization of the UCNPs@DNAzyme was conducted using XRD, TEM, FT-IR and
fluorescence testing, and it was shown that the β-NaYF4: Yb, Tm, Gd nanoparticles were successfully prepared (∼27 nm). Upon 980 nm excitation, the UCNPs@DNAzyme emitted green light at λ=475 nm. Furthermore, Förster resonance energy transfer was observed between
the UCNPs@DNAzyme (doner) and uranyl (acceptor) present on the substrate chain. This resulted in fluorescence quenching, which was used to quantify the concentration of uranyl. The developed method was found to be highly selective and sensitive, with a detection limit as low as 43 nM. This
method also demonstrated excellent specificity and sensitivity for the determination of uranyl in water samples, indicating its potential application in near-infrared fluorescence detection and imaging of uranyl in vivo.
研究领域:
分析化学
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材料科学
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环境科学
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纳米技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Yongmei Wu,Kai Sheng,Yongqin Liu,Qian Yu,Baoxian Ye
In this work, an ultrasensitive electrochemical microRNA detection strategy was developed based on porous palladium-modified horseradish peroxidase sphere ( [email protected] ) and target-induced assembly of DNAzyme. A highly loaded HRP sphere was prepared by covalent layer-by-layer assembly with CaCO 3 as sacrificial template for the first time, and was further modified with porous Pd. Notably, [email protected] composite showed a good redox activity of HRP and electrocatalytic activity toward H 2 O 2 . The utilization of [email protected] as electrochemical signal indicator and enhancer to fabricate biosensor could avoid the need for additional redox mediator and amplify the detection sensitivity. Moreover, target recycling amplification was achieved by Pb 2+ -induced cleavage of ternary “Y” structure, circumventing the use of labile nuclease . Subsequent DNA concatamer synthesized through rolling circle amplification (RCA) reaction with cleaved hairpin probe as primer, hybridized with plentiful [email protected] probes, which led to the increased loading of redox-active and electrocatalytic [email protected] for sensitivity improvement. So the proposed electrochemical biosensor detected miRNA-24 down to 0.2 fM (S/N = 3) with a wide linear range from 3 fM to 1 nM. With bifunctional [email protected] tag, DNAzyme-aided target recycle and programmable junction probe, this strategy possessed the advantages of high efficiency, high sensitivity, low cost and versatility, and thus held great promise for other low-abundance nucleic acids determination.
研究领域:
分析化学
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生物化学
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分子生物学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Jing Xu,Yinbing liu,Xinqi Luo,Yujin Li,Yiqing Xing,Ke-Jing Huang
Self-powered electrochemical biosensors based on enzyme biofuel cells (EBFC) are uniquely valuable in developing real-time and on-site detection of heavy metal ions. This work combines graphdiyne/graphene (GDY/GR) heterojunction with Deoxyribozyme (DNAzyme) trigger catalytic hairpin self-assembly (CHA) circuit. In addition, the smartphone is introduced to obtain the detection signals. The results show that the designed sensor platform can be used for single-tube ultrasensitive and visual lead ion detection with a wide linear range of 0.003–5000 nM and a low detection limit (LOD) of 0.005 nM. This design enriches the routine screening methods for heavy metal ions in food and environmental samples and introduces new idea for the development and application of self-powered electrochemical biosensors.
研究领域:
生物传感技术
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环境科学
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材料科学
·
分析化学
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产品关联
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 18
·5643-5649
·
IF 4.616
Li Tan,Jiangnan Lu,Xin Wang,Guang Liu,Xiaomei Mu,Kun Hu,Shulin Zhao,Jianniao Tian
Highly specific and ultrasensitive detection of uracil-DNA glycosylase (UDG) activity is of great significance for maintaining genomic integrity and medical research of related diseases. Here, we constructed a random DNA walking nanomachine based on a DNAzyme for UDG activity detection on the AuNP (Au nanoparticle) surface. When UDG is present, the U bases in the Y structure are removed, resulting in AP sites, which will be cleaved by Endo-IV to generate a 3′ concave end for Exo-III, causing the locking strand of the DNAzyme to be completely hydrolyzed by the Exo-III and release the walking strand to randomly pair with the substrate strand on the AuNP surface; then, the walking strand exerts its cleavage activity with the assistance of Mg2+ to cleave the substrate strand and keep the fluorophore 6-carboxyfluorescein (FAM) away from the surface of the AuNP, which restores the fluorescence signal of this system. In this way, sensitive detection of UDG can be realized, and the detection limit is as low as 3.69 × 10−6 U mL−1. In addition, we found that this method is highly specific to UDG and can be used to detect UDG specifically in complex samples, which has certain application prospects in biomedical research and clinical diagnosis related to UDG.
研究领域:
分子生物学
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纳米技术
·
生物医学工程
·
分析化学
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DOI
PubMed
高影响因子
ANALYST
·
2017
·Vol. 142
·期 11
·2013-2019
·
IF 3.885
Hui Zhang,Zhihui Guo,Huilei Dong,Hongfei Chen,Chenxin Cai
In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (ΔI) allowed detection of DNMT1 activity down to 0.09 U mL−1, and ΔI was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL−1 in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells. This ECL assay provides a promising platform for profiling of the mutational cells of tumors and shows a great potential for application to DNA methylation-related clinical diagnostics.
研究领域:
生物化学
·
分子生物学
·
临床诊断学
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-9
·
IF 5.705
Wang Jing,Li Haigang,Li Tingting,Ling Liansheng
A one-step, one-tube colorimetric assay is described for the detection of bacterial double-stranded DNA (dsDNA). It utilizes a G-quadruplex DNAzyme produced by an asymmetric polymerase chain reaction (As-PCR) that catalyzes the oxidation of cysteine to form cystine. This results in the formation of oligonucleotide-modified gold nanoparticles via triplex formation, and eventually in a color change from red to blue that occurs within about 10 mins. This can be measured by ratiometric colorimetric (at 525 and 600 nm). The limit of detection (LOD) for the model analyte (dsDNA of Staphylococcus aureus ( S. aureus )) is as low as 0.28 pg per 0.05 mL with a good linear response ranging from 16.0 fg·μL −1 to 1.6 ng·μL −1 . This is much lower than previously reported LODs. The assay is highly selective for S. aureus dsDNA over a range of other bacterial DNAs. Conceivably, it provides an attractive alternative tool for rapid detection of bacterial dsDNA as required in pathogen screening in the food industry.
Schematic presentation of a colorimetric assay for bacterial DNA. It is based on the catalytic activity of a G-quadruplex DNAzyme that is formed by an asymmetric PCR involving triplex DNA formation and gold nanoparticle (AuNPs) aggregation.
研究领域:
分子生物学
·
纳米生物技术
·
病原体检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Taorong Shen,Xing Huang,Yanfei Zhang,Yanli Tong,Yakun Shi,Jianhe Guo,Xiaoyong Zou,Yuzhi Xu,Zong Dai
Fat mass and obesity-associated protein (FTO) plays a crucial role in regulating the dynamic modification of N6-methyladenosine (m6A) in eukaryotic mRNA. Sensitive detection of the FTO level and efficient evaluation of the FTO demethylase activity are of great importance to early cancer diagnosis and anticancer drug discovery, which are currently challenged by limited sensitivity/precision and low throughput. Herein, a robust strategy based on the dephosphorylation switch DNAzyme-rolling circle amplification (RCA) circuit, termed DSD-RCA, is developed for highly sensitive detection of FTO and inhibitor screening. Initially, the catalytic activity of DNAzyme is silenced by engineering with an m6A modification in its catalytic core. Only in the presence of target FTO can the methyl group on DNAzyme be eliminated, resulting in the activation of the catalytic activity of DNAzyme and thus cleaving the hairpin substrate to release numerous primers. Different from the conventional methods that use the downstream cleavage primer with the original 3′-hydroxyl end directly as the RCA primer with the problem of high background signal, which should be compensated by additional separation and wash steps in heterogeneous format, our DSD-RCA assay uses the upstream cleavage primer with a 2′,3′-cyclic phosphate terminus at the 3′-end serving as an intrinsically blocked 3′ end. Only after a dephosphorylation reaction mediated by T4 polynucleotide kinase can the upstream cleavage primers with a resultant 3′-hydroxyl end be extended by RCA. With the high signal-to-noise ratio and homogeneous property, the proposed platform can sensitively detect FTO with a limit of detection of 31.4 pM, and the relative standard deviations (RSDs %) ranging from 0.8 to 2.0% were much lower than the heterogeneous methods. The DSD-RCA method was applied for analyzing FTO in cytoplasmic lysates from different cell lines and tissues of breast cancer patients and further used for screening FTO inhibitors without the need for separation or cleaning, providing an opportunity for achieving high throughput and demonstrating the potential applications of this strategy in disease diagnostics, drug discovery, and biological applications.
研究领域:
生物化学
·
分子生物学
·
癌症研究
·
药物发现
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Yiming Xie,Haiyan Wang,Xinyue Yuwen,Guosong Lai
Herein, we combine the exonuclease III (Exo III)-catalyzed release of a Zn2+-dependent ligation DNAzyme with the DNAzyme-driven strand displacement reaction (SDR) to develop a novel homogeneous colorimetric bioassay method for kanamycin (Kana) antibiotic detection. Upon the biorecognition reaction between Kana and a designed hairpin DNA, the DNAzyme-containing strand can be catalytically released by Exo III. Then, this DNAzyme will catalyze the ligation of two oligonucleotides to cause a SDR and the aggregation of gold nanoparticles (Au NPs) labeled by two linker DNA strands. Due to the aggregation of Au NPs for colorimetric signal transduction and the Exo III and SDR-assisted dual signal amplification, this method shows a wide linear range of 5 orders of magnitude and a very low detection limit down to 8.1 fg mL–1. Together with its excellent selectivity, repeatability, reliability, and convenient manipulation, the proposed method shows a great potential for the food quality monitoring application.
研究领域:
分析化学
·
生物化学
·
纳米技术
·
食品科学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Shuang Zhao,Xuesong Wang,Ruijia Deng,Xiaoqi Tang,Zuowei Xie,Ping Huang,Xianlan Wu,Jie Luo,Yu Tang,Jing Sheng,Sha Yang,Ming Chen,Kai Chang
Synthetic catalytic DNAzyme circuits have gained great attention as versatile theranostic toolboxes, but their accuracy and efficiency is restricted by uncontrolled signal leakage and inefficient circuitry activation. Here, a trinity DNAzyme circuit (TriDC) is constructed using a unique logic gate bioswitchable DNA tetrahedral walker@MnO 2 nanosheets structure. The AND logic gate bioswitchable DNA tetrahedral walker, composed of dual allosteric DNAzyme (termed “commander” and “soldier”) and tetrahedral scaffold, is used to execute imaging-guided gene therapy by avoiding undesired signal leakage and cascade “walking” in a confined space. Glutathione-mediated reduction of MnO 2 nanosheets provides secondary confinement to stack the DNA tetrahedral walker in situ, offering abundant Mn 2+ as a cofactor to achieve self-sufficiency of DNAzyme circuits and induce chemo-dynamic therapy. The structure increases local reaction concentrations by ≈100-fold according to the collision frequency model, enabling the AND logic detection of microRNA-10b and microRNA-155 at a limit of detection of 98.97 p m , thereby facilitating the precise imaging of breast cancer cells. The combined gene/chemo-dynamic therapy achieves 66.2% tumor growth inhibition efficiency. The TriDC offers novel insights into tumor theranostics and also presents a paradigm for the in situ implementation of higher-order DNAzyme circuits.
研究领域:
纳米技术
·
生物医学工程
·
诊疗学
·
癌症研究
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Qianhong Chen,Jinglan Cao,Yi Zhao,Ruo Yuan,Yun Xiang
The fat mass and obesity-associated protein (FTO) is vital for modulating dynamic reversible process of N6-methyladenosine (m 6 A) level in the nucleus and the abnormal expression of FTO correlates with generation and development of Alzheimer's disease, obesity and several cancers. The sensitive detection of FTO is therefore of significance for diagnosis of FTO-related diseases. In this work, on the basis of the demethylation enzyme capability of FTO and the dual Mg 2+ -dependent DNAzyme recycled signal magnification strategy, establishment of a highly sensitive electrochemical FTO biosensor is described. The target FTO molecules catalyze the demethylation of m 6 A-modifed sequences to form effective cleavage substrates for the MboI endonuclease, leading to the release of active DNAzyme sequences to trigger subsequent dual DNAzyme recycling cycles. Many methylene blue-tagged ssDNA sequences can thus be yielded via the DNAzyme amplifications and captured on the sensor electrode to generate highly amplified currents for detecting FTO in range of 0.04 pM to 10 nM with 6.11 fM detection limit. Additionally, the sensor demonstrates high selectivity and is able to be used for detecting cell lysate FTO, showing its potential for the diagnosis and study of FTO-related diseases for various application purposes.
研究领域:
生物化学
·
分子生物学
·
分析化学
·
生物医学工程
·
诊断学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）