JOURNAL OF APICULTURAL RESEARCH
·
2023
·
IF 1.9
Olaoye Solomon Balogun,Anthonia Olufunke Oluduro,Zhiqiang Liu
Volatile oils of propolis obtained from Ondo (Ondo town) and Osun (Ile-Ife) States in Rain forest region of southwest Nigeria were extracted using the hydrodistillation method. The oils were analysed on GC/MS and investigated for DPPH scavenging activities, antibacterial potentials, and inhibitory activities on lipase and alpha glucosidase enzymes. Spectrophotometric methods were used for the DPPH scavenging and enzyme’ inhibitions assays while the agar well diffusion method was employed for the antibacterial test. GC/MS analysis of essential oils obtained from Ondo (BPO) and Ile-Ife (BPI) indicated 80 (97.82%) and 66 (99.31%) compounds respectively. Upon classification, both oils were rich in monoterpenes, sequisterpenes and non-isoprenoid hydrocarbons. Principal chemical components of BPO included trans-α-bergamotene (9.08%), α-copaene (5.83%), cadina-1,4-diene (4.04%), 2-methyl-5-propionylfuran (3.43%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (3.52%), benzaldehyde (2.98%), sulcatone (2.95%), γ-muurolene (2.51%), caryophyllene (2.40%), α-bisabolol (2.09%), α-eudesmol (2.07) and α-curcumene (2.06%) while those of BPI were trans-α-bergamotene (10.14%), citral (5.44%), α-copaene (5.25%), cadina-1,4-diene (5.09%), melonal (3.69%), sulcatone (3.56%), farnesol (2.71%), 3,5,9-trimethyl-deca-2,4,8-trien-1-ol (2.66%), α-curcumene (2.64%), 1,5,9,9-tetramethyl-1,4,7-cycloundecatriene (2.20%), and α-muurolene (2.20%). Both oils inhibited α-glucosidase and had DPPH scavenging activities better than the standards, however, anti-lipase activity was observed for BPI only. The oils also demonstrated broad spectrum of antibacterial activities.The volatile components of Nigerian bee propolis are being investigated for the first time, and the plethora of biological activities resulting from their diverse chemical composition showed that bee propolis could be a potential source for drug discovery.
研究领域:
药理学
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植物化学
·
微生物学
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DOI
高影响因子
MOLECULES
·
2023
·Vol. 28
·期 17
·6176
·
IF 4.6
Jianfa Wu,Lei Li,Chang Liu,Chunyi Li,Ying Cui,Weixing Ding,Jing Zhang,Leiling Shi
Two new compounds named 4,4′-bis(β-D-glucopyranosyloxy)biphenyl (1) and spirostane-25(27)-en-2α,3β-diol-3-O-β-D-xylopyranosyl(1→3)-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside (2) were isolated from n-butanol extraction part of 80% ethanol extract of Allii Macrostemonis Bulbus. Alongside these, ten known compounds (3–12) were also identified, including a flavonoid glycoside (3), seven steroids (4–10), a nucleoside (11), and a phenylpropanoid glycoside (12) were found. Notably, compounds3–6were isolated from this plant for the first time. The structures of all compounds were confirmed using high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), 1D, and 2D NMR spectroscopy. Some of these compounds showed strong antioxidant activity, and compound1demonstrated the most potent reduction of ferric ions (Fe3+) with an IC50value of 0.59 ± 0.18 mg/mL. Compounds2and3exhibited the highest scavenging activity against superoxide anion radicals (O2−·) with an IC50value of 0.02 ± 0.01 mg/mL. Additionally, compound3displayed substantial scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) with IC50values of 0.21 ± 0.17 mg/mL and 0.02 ± 0.01 mg/mL, respectively. The discovery of these two new compounds is a reference for identifying Allii Macrostemonis Bulbus quality markers. Moreover, their exceptional antioxidant activity offers a promising avenue for uncovering novel natural antioxidants.
研究领域:
药用植物学
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天然产物化学
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药物开发
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiandong Shen,Bijiang Zhong,Lijun Fu,Bingyan Liu,Wenshui Xia,Qixing Jiang
Aquatic animals are vital source for peptides, while information on antioxidant peptides of Chinese softshell turtle is scarce. The objective of this study was to optimize the production of turtle protein hydrolysate (PTH) by proteinase hydrolysis and evaluate its functional property and antioxidant activity. Results revealed that TPH from turtle protein was hydrolysed using protamex. Under the optimum condition (enzyme-substrate ratio of 3500 U/g, pH 7.0, temperature of 50 °C, and 4 h), TPH had the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, with the degree of hydrolysis (DH) of 21.27 ± 1.32%. Obtained TPH contained a high percentage of essential amino acids and its molecular weight of<3000 Da accounted for 99.61%. The solubility of TPH was more than 80% when pH was below 4.0 or above 5.0, and there was no significant difference in its solubility when temperature was 40–100 °C. Meanwhile, TPH exhibited higher emulsifying activity index and the emulsion stability index at pH 7.0 and 10.0. Antioxidant activity studies showed that DPPH scavenging activity, reducing power and ferrous chelating activity of TPH were attained depending on the concentration of hydrolysate. All results indicated that the functional and antioxidant properties of TPH could be beneficial for its application in functional food.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
TRANSACTIONS OF TIANJIN UNIVERSITY
·
2015
·Vol. 21
·期 5
·433-439
·
IF 0
Zhu Hongji,Wang Shipeng,Tian Li,Zhang Hua
Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes(Papain, Pepsin, Trypsin and Neutrase, respectively)were investigated. The Trypsin hydrolysate obtained by hydrolysis exhibited the highest 1,1-dipheny-l-2-picrylhydrazyl(DPPH)radical scavenging activity(DRSA)compared with other hydrolysates. Response surface method ology(RSM), based on Box-Behnken design, was used to study the influence of hydrolysis conditions on the DRSA. The optimal hydrolysis conditions were as follows: pH 7.38, temperature 48.2°C and enzyme/substrate(E/S)ratio 2 840 U/g. Under these conditions, the maximum DRSA was(22.85 ± 0.57)%,. The experimental values agreed with the value (23.09%,)predicted by the model within a 95%, confidence interval. By using gel filtration chromatography and reversed-phase high performance liquid chromatography(RP-HPLC), antioxidant peptide(D2-P)was isolated from flatfish skin protein hydrolysates(FSPH)and could exhibit a(54.28 ± 1.37)%, scavenging activity on DPPH radical at the concentration of 5 mg/mL. This is the first report of a scientific basis for the preparation of antioxidant peptides from flatfish skin. The results suggested that the antioxidant peptides can be exploited into functional foods or used as a novel source of nutraceuticals.
研究领域:
食品科学
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生物化学
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产品关联
DOI
Antioxidants
·
2024
·Vol. 13
·期 8
·910
·
IF 6
Lingxia Chen,Lijun Wang,Lifu Ma,Chao Wang,Xinshu Qin,Minlong Wang,Xiaohe Zhang,Ruoyan Yang,Bing Fang,Jie An
The incorporation of antioxidants in food products is essential to prevent or delay deterioration, thereby addressing food spoilage. Thiol compounds, recognized for their natural antioxidant properties, are widely used in various foods; however, their antioxidant capacity is often limited. This study investigates the potential enhancement of thiol antioxidant capacity through the addition of a soluble, low-toxic inorganic Sm-cluster. Our findings demonstrate that the Sm-cluster significantly bolsters the antioxidant efficacy of thiol compounds. We explored, for the first time, the in vitro antioxidant activities of an Sm-oxo/hydroxy cluster combined with a cysteine derivative for potential food applications. The composition exhibited a robust inhibition of aromatic aldehyde flavor compound oxidation and displayed strong, dose-dependent DPPH (2,2-diphenyl-1-picrylhydrazine) radical scavenging activity. Notably, the antioxidant activity of the Sm-cluster/cysteine derivative was further enhanced under strong visible light conditions, which typically increased the likelihood of oxidation. These results suggest that the combination of inorganic cluster and thiol compounds presents a promising natural alternative to traditional antioxidants in the food industry.
研究领域:
食品科学
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化学
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营养学
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 15
·2769
·
IF 3.06
Xican Li,Xiaojian Ouyang,Minshi Liang,Dongfeng Chen
The biological process, 3-O-galactosylation, is important in plant cells. To understand the mechanism of the reduction of flavonol antioxidative activity by 3-O-galactosylation, myricetin-3-O-galactoside (M3OGa) and myricetin aglycone were each incubated with 2 mol α,α-diphenyl-β-picrylhydrazyl radical (DPPH•) and subsequently comparatively analyzed for radical adduct formation (RAF) products using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS) technology. The analyses revealed that M3OGa afforded an M3OGa–DPPH adduct (m/z873.1573) and an M3OGa–M3OGa dimer (m/z958.1620). Similarly, myricetin yielded a myricetin–DPPH adduct (m/z711.1039) and a myricetin–myricetin dimer (m/z634.0544). Subsequently, M3OGa and myricetin were compared using three redox-dependent antioxidant analyses, including DPPH•-trapping analysis, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-trapping analysis, and•O2inhibition analysis. In the three analyses, M3OGa always possessed higher IC50values than those of myricetin. Conclusively, M3OGa and its myricetin aglycone could trap the free radical via a chain reaction comprising of a propagation step and a termination step. At the propagation step, both M3OGa and myricetin could trap radicals through redox-dependent antioxidant pathways. The 3-O-galactosylation process, however, could limit these pathways; thus, M3OGa is an inferior antioxidant compared to its myricetin aglycone. Nevertheless, 3-O-galactosylation has a negligible effect on the termination step. This 3-O-galactosylation effect has provided novel evidence that the difference in the antioxidative activities of phytophenols exists at the propagation step rather than the termination step.
研究领域:
植物化学
·
生物化学
·
分析化学
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DOI
高影响因子
JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 3
·e70125
·
IF 3.4
Guorui Yang,Jingru Zhao,Yaping Li,Mengqi Teng,Shaoxuan Yu,Yuanda Song,Haifang Xiao
Raspberry possesses several health benefits such as antitumor, antioxidant, and blood sugar-regulating activities. In order to improve the use value of raspberries, the optimal preparation process of raspberry chewable tablets (RCT) and its physical, chemical, antioxidant, and hypoglycemic properties were investigated in this research. The optimum formulation of RCT was determined as 39.1% raspberry powder, 13.1% red beet root powder, 9.8% corn starch, 15% microcrystalline cellulose, 14% mannitol, 1% magnesium stearate, and 8% ethanol (75%) by single-factor experiment and response surface methodology using sensory evaluation score and hardness as parameters. RCT prepared using the optimal formulation had a hardness of 47.33 ± 2.31 N, brittleness of 0.33%, weight of 1.00 ± 0.03 g, and a color difference Δ E = 0.427. The total phenolic and total flavonoid contents of RCT were 18.610 ± 0.483 mg/g and 3.885 ± 0.061 mg/g, respectively. Meanwhile, seven phenolic compounds in RCT were determined by high-performance liquid chromatography. Free radical scavenging abilities ((1,1-diphenyl-2-picrylhydrazyl, DPPH), (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS), and ·OH), ferric ion-reducing antioxidant power and cell tests proved the significant antioxidant ability of RCT. The obvious inhibitory activities of RCT on α-glucosidase and α-amylase reflected its hypoglycemic potential. This study provided an important basis for the development of raspberry functional foods with antioxidant and blood glucose regulating potential.
研究领域:
食品科学
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营养学
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药理学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xingzhu Tang,Lingling Wang,Yajie Zhang,Chaofan Sun
Antioxidant activity, as a topic of current interest, is discussed together with the excited state intramolecular proton transfer (ESIPT) process for three flavonoid derivatives, based on density functional theory (DFT)and time-dependent DFT (TD-DFT) methods, as well as DPPH free radical scavenging assay. The potential energy curves and transition states demonstrate that the three molecules can undergo only single proton transfer in the excited state, and all of them are ultrafast ESIPT processes. The absorption spectra of all the molecules show effective protection against UV radiation with low fluorescence intensity, especially Baicalein (Bai), which demonstrates their great potential for sunscreen applications. The density of states, HOMO energy values, global and local indices reveal that the antioxidant activity of the molecules after ESIPT process is enhanced, with Bai having the highest antioxidant activity, which is significantly attributed to the number and position of phenolic hydroxyl groups. Moreover, by comparing the DPPH free radical scavenging activity under the dark and UV radiation conditions, the radical scavenging activity (RSA) value in the UV radiation is remarkably higher than that in the dark condition, in which Bai achieves RSA value of 93.4%. Overall, the antioxidant activity of all three ESIPT-based flavonoid derivatives, especially Bai, is significantly elevated in the keto* form, which reinforces the significant relationship between antioxidant activity and ESIPT process, and provides new application prospects for molecules with ESIPT properties.
研究领域:
化学
·
生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2023
·
IF 4.1
Yingnan Liu,Fei Tong,Yingran Xu,Yunyun Hu,Wenya Liu,Zan Yang,Zhenyu Yu,Guoyuan Xiong,Yibin Zhou,Yaqing Xiao
BACKGROUND Active and smart food packaging based on natural polymers and pH-sensitive dyes as indicators has attracted widespread attention. In the present study, an antioxidant and amine-response color indicator film was developed by incorporating bilirubin (BIL) into the κ-carrageenan (Carr) matrix. RESULTS It was found that the introduction of BIL had no effect on the crystal/chemical structure, water sensitivity and mechanical performance of the Carr-based films. However, the barrier properties to light and the thermal stability were significantly improved after the addition BIL. The Carr/BIL composite films exhibited excellent 1,1-diphenyl-2-picryl-hydrazyl (i.e. DPPH)/2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (i.e. ABTS) free radical scavenging abilities and color responsiveness to different concentrations of ammonia. The application assay reflected that the Carr/BIL 0.0075 film was effective in delaying the oxidative deterioration of shrimp during storage and realizing the color response of its freshness through the change of b * value. CONCLUSION Active and smart packaging films were successfully prepared by incorporating different contents of BIL into the Carr matrix. The present study helps to further encourage the design and development of a multi-functional packaging material. © 2023 Society of Chemical Industry.
研究领域:
食品科学
·
材料科学
·
化学
·
包装工程
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产品关联
DOI
PubMed
高影响因子
NEUROCHEMICAL RESEARCH
·
2022
·Vol. 48
·期 2
·579-590
·
IF 4.414
Shen Mengya,Zheng Yuantie,Li Ge,Chen Yinqi,Huang Lili,Wu Jianzhang,Hong Chenglv
Abstract Antioxidants represent a potential therapy for cerebral ischemia–reperfusion injury (CIRI). Compounds which exhibit both direct and indirect antioxidative activity may potentially exert improved effects. Hence, we aimed to assess whether the dual antioxidant DH-217, a derivative of DHAP clinically used to treat coronary heart disease, can reduce oxidative stress damage and elucidate the underlying mechanism. Hydrogen peroxide (H 2 O 2 )-induced and Middle Cerebral Artery Occlusion (MCAO)-induced damages were used to imitate oxidative stress. The antioxidation of DH-217 was determined by MTT, ROS, colony and DPPH assay. Besides, immunofluorescence, Real-Time PCR Analyses, western blotting and si-RNA/Plasmid-induced protein expression were used for mechanism validation. DPPH scavenging assay evidenced DH-217 was a well free radical scavenger. Cell survival assay also showed that DH-217 had a significant cytoprotection through direct and indirect clearance mechanisms. Further, it clearly inhibited oxidative stress-induced IkappaB kinase beta (IKKβ) phosphorylation and increased heme oxygenase-1 (HO-1) expression. Significantly, these antioxidant beneficial effects were reversed by HO-1 inhibitor, si-nuclear erythroid 2-related factor 2 (Nrf2) and IKKβ plasmid. Meanwhile, DH-217 had a good neuroprotective effect on CIRI rats. The dual antioxidant DH-217 has potential reference value for drug development of CIRI. Furthermore, inhibition of IKKβ phosphorylation and activation of Nrf2/HO-1 could be a promising antioxidant pathway. Graphical Abstract Dual antioxidant DH-217 not only has the ability of directly scavenging ROS, but also can clear it by targeting IKKβ/Nrf2/HO-1 signal axis. Inhibition of IKKβ phosphorylation and activation of Nrf2/HO-1 may be a promising antioxidant pathway for CIRI.
研究领域:
药理学
·
神经科学
·
分子生物学
·
细胞生物学
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产品关联
DOI
PubMed
高影响因子
ULTRASONICS SONOCHEMISTRY
·
2024
·
IF 8.7
Ying Pan,Chunjuan Liu,Shuo Jiang,Lili Guan,Xinyao Liu,Liankui Wen
In the current study, a novel crude polysaccharide (cNCEP) was extracted from N. commune Vaucher utilizing ultrasonic-assisted extraction (UAE) with 60 % ethanol, employing response surface methodology. The optimal yield of cNCEP was determined to be 8.07 ± 0.08 mg/g, achieved through ultrasonic-assisted extraction under the conditions of a material-to-liquid ratio of 1:22, temperature of 56 °C, power of 570 W, and duration of 147 min. Subsequent purification of NCEP via Sephadex G75 resulted in a novel polysaccharide with a molecular weight of 20.466 kDa. NCEP exhibited significant scavenging activites against DPPH and hydroxyl radicals, as well as notable in vitro immunomodulatory properties. Furthermore, the mechanisms underlying the immunomodulatory effects of NCEP, involving enhancement of immunity, were investigated, revealing potential regulation of MAPK and TLR4-IRF7-NF-κB signaling pathways through RNA-Seq and Western blot analyses. These findings highlight the promising potential of NCEP as an organic immunomodulatory agent and functional food ingredient.
研究领域:
生物化学
·
免疫学
·
药理学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2018
·
IF 5.158
Zheng-Nan Cai,Wan Li,Shomaila Mehmood,Wen-Juan Pan,Ya Wang,Fan-Ju Meng,Xiao-Fang Wang,Yong-Ming Lu,Yan Chen
This study aimed to investigate the structural features, in vitro and in vivo antioxidant activities of a heteropolysaccharide from the fruiting bodies of Morchella esculenta (FMP-1). FMP-1 had an average molecular weight of 4.7 × 10 3 Da and consisted of mannose, glucose and galactose. By methylation and NMR analysis, the backbone of FMP-1 was deduced to be made up of 1,4-linked Glc p and 1,6-linked Gal p . Hydroxyl, DPPH and superoxide radicals could be efficiently scavenged by FMP-1, with IC 50 values of 74.26, 119.32 and 161.49 μg/mL, respectively. Furthermore, FMP-1 could significantly protect zebrafish embryos against AAPH-induced oxidative damage. Decrease in malformations and mortalities was observed along with the reduction of ROS production, NO production and cell death. The protective effects were by decreasing MDA content and increasing SOD, CAT and GSH-Px levels. The current work provided a good suggestion of the potential utilization of FMP-1 as an attractive natural antioxidant.
研究领域:
药理学
·
生物化学
·
真菌学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）