ENVIRONMENTAL POLLUTION
·
2024
·
IF 7.6
Yanan Sun,Ping Ding,Jiayi Zhang,Kexin Sun,Xintong Li,Qing Ge,Yao Dang,Yunjiang Yu,Guocheng Hu
Microplastics (MPs) pollution, together with its consequential effect on aquatic biota, represent a burgeoning environmental concern that has garnered significant scholarly attention. Thiamethoxam (TMX), a prevalently utilized neonicotinoid insecticide, is renowned for its neurotoxic impact and selective action against targeted pests. The aquatic environment serves as a receptacle for numerous pollutants, such as MPs and neonicotinoid insecticides. However, there is currently a lack of comprehensive understanding regarding the toxic effects of co-exposure to aged MPs and neonicotinoid insecticides in aquatic organisms. Therefore, we endeavor to elucidate the deleterious impacts of aged polystyrene (PS) and TMX on zebrafish ( Danio rerio ) larvae when present at environmentally relevant concentrations, and to reveal the underlying molecular mechanisms driving these effects. Our study showed that exposure to aged PS, TMX, or their combination notably inhibited the heart rate and locomotion of zebrafish larvae, with a pronounced effect observed under combined exposure. Aged PS and TMX were found to diminish the activity of antioxidative enzymes (SOD, CAT, and GST), elevate MDA levels, and disrupt neurotransmitter homeostasis (5-HT, GABA and ACh). Notably, the mixtures exhibited synergistic effects. Moreover, gene expression related to oxidative stress (e.g., gstr1, gpx1a, sod1, cat1, p38a, ho-1, and nrf2b ) and neurotransmission (e.g., ache, ChAT, gat1, gabra1, 5ht1b, and 5ht1aa ) was significantly altered upon co-exposure to aged PS and TMX in larval zebrafish. In summary, our findings support the harmful effects of aged MPs and the neonicotinoid insecticides they carry on aquatic organisms. Results from this study enhance our understanding of the biological risks of MPs and insecticides, as well as help fill existing knowledge gaps on neonicotinoid insecticides and MPs coexistence toxicity in aquatic environment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2025
·
IF 3.5
Meng Li,Mengai He,Zhenfeng Liao,Chenxi Li,Yingqiu Mi,Ningning Pan,Xinyi Xie,Zhiyu Liu,Jiayin Hou,Xueli Yu,Liezhong Chen
Ochratoxin A (OTA) has been increasingly detected in foodstuffs and human samples. However, the molecular mechanisms of developmental toxicity induced by OTA remain poorly explored. Our results demonstrated that oxidative stress induced by OTA caused mitochondrial dysfunction in larvae, characterized by malformed mitochondria, and decreased mitochondrial membrane potential and complex II, IV and V activities. Central carbon metabolism analysis indicated that exposure to OTA induced energy metabolism disturbance by decreasing ATP contents and altering energy-relevant metabolite contents. Energy metabolism disturbance was further confirmed by transcriptomics analysis, with differentially expressed genes significantly enriching in glycolysis and TCA cycle. Additionally, molecular docking simulations and surface plasmon resonance analysis demonstrated that OTA interacted with mfn1 and mfn2, verifying its interference effects on mitochondrial dynamics. The insufficient energy supply induced by OTA activated AMP-activated protein kinase signal pathways, which accelerated catabolic processes, accompanied by decreases in yolk sac size and yolk lipid. Finally, insufficient energy supply caused developmental retardation of zebrafish larvae. Collectively, this study systematically elucidated the roles of mitochondrial dynamics imbalance and energy dyshomeostasis in the developmental toxicity induced by OTA, providing critical insights for refining food safety standards and therapeutic interventions.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Xuchun Qiu,Zhen Su,Jiarui Gao,Yiming Cui,Kejun Dong,Kun Chen,Ru-Jin Zhao,Songmei Wang,Tao Wu,Yanhong Shi
Thimerosal (THI) is the most widely used form of organic mercury in pharmaceutical and personal care products, and has become a major source of ethylmercury pollution in aquatic ecosystems. However, knowledge about its potential risk to aquatic species is limited. In this study, zebrafish were exposed to THI for 7 days, and variations in their behavioral traits, brain monoaminergic neurotransmitter contents, and related gene expression were investigated. After the 7-day exposure, THI reduced locomotor activity and thigmotaxis in males but not females. Exposure to THI increased the social interaction between females but decreased that between males. The THI exposure also significantly reduced the serotonin (5-HT), 5-hydroxyindoleacetic acid, dopamine (DA), and 3,4-dihydroxyphenylacetic acid contents in the brain of males, but only significantly decreased the DA content in females. Correlation analysis revealed that the neurochemical alterations in the brain of zebrafish play critical roles in the behavioral abnormalities induced by THI exposure. Moreover, THI also significantly altered the expression of some genes associated with the synthesis, metabolism, and receptor binding of 5-HT and DA in the brain of zebrafish. The differences in these gene expressions between female and male zebrafish exposed to THI seem to be an important mechanism underlying their sex-specific responses to this chemical. This is the first report on the sex-specific effects of THI on behaviors and brain monoaminergic neurotransmitter contents in zebrafish, which can further improve our understanding of its toxic effects on teleost.
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Kun Qiao,Tiantian Hu,Yao Jiang,Jianping Huang,Jingjin Hu,Wenjun Gui,Qingfu Ye,Shuying Li,Guonian Zhu
Chlorpyrifos is a widely used organophosphate insecticide and ubiquitously detected in the environment. However, little attention has been paid to its endocrine disrupting effect to non-target organisms. In the present study, zebrafish was exposed to 13 and 65 μg/L of chlorpyrifos for 7 and 10 days to determine the induced neurotoxicity and the alteration of thyroid metabolism. The 120 h LC 50 and LC 10 of chlorpyrifos was estimated as 1.35 mg/L and 0.62 mg/L based on the acute embryo toxicity assay, respectively. The acetylcholinesterase (AChE) inhibitory was detected by 13 μg/L chlorpyrifos and could be reversed by the co-exposure of 100 and 1000 μg/L anticholinergic agent atropine. For thyroid hormone level, 13 and 65 μg/L of chlorpyrifos induced increased free T 3 levels in 10 dpf (days post-fertilization). The expression of thyroid related genes in 7 and 10 dpf exposed zebrafish were measured by the quantitative Real-Time PCR (qRT-PCR) assay. The mRNA expression of tshba , thrb , crhb , ttr , tpo , ugt1ab and slc5a5 had significant change. However, the alterations of thyroid hormone and mRNA expression could be partly rescued by the addition of atropine. The molecular docking of chlorpyrifos and T 3 to the thyroid receptor β in zebrafish using homology modelling and CDOCKER procedures shown weaker binding ability of chlorpyrifos compared to T 3 . Therefore, we concluded that the disturbance of thyroid signaling in zebrafish might arise from the developmental neurotoxicity induced by chlorpyrifos.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2026
·
IF 10.6
Xunyue Liu,Peng Liu,Haojie Zhu,Junyao Ge,Qiong Rao,Jianqiang Sun
Spirotetramat, a broad-spectrum tetronic acid insecticide, is widely used in agriculture because of its high insecticidal efficacy. However, its intensive and prolonged use may result in environmental accumulation and potential risks to non-target organisms. Although previous studies have demonstrated that spirotetramat induces developmental, reproductive, and hepatic toxicity in zebrafish, its neurotoxic effects on the brain and the underlying mechanisms remain poorly understood. Therefore, this study aimed to systematically evaluate the neurotoxicity of spirotetramat in zebrafish and to explore the potential molecular mechanisms involved.Histopathological examination, behavioral analysis, neurotransmitter quantification, transcriptomic profiling, and molecular docking were performed. Spirotetramat exposure caused obvious histopathological damage in the zebrafish brain, including vacuolation and cellular degeneration, and induced abnormal locomotor behaviors, as evidenced by increased swimming speed and distance, together with enhanced thigmotaxis, geotaxis, and scototaxis. In addition, spirotetramat disrupted neurotransmitter homeostasis, characterized by elevated DOPAC and HK levels and reduced Pun levels. Transcriptomic analysis revealed significant alterations in pathways related to visual transduction and neural regulation, accompanied by upregulated expression of key visual genes, including rho , pde6a , and saga . Molecular docking suggested potential interactions between spirotetramat and visual-related proteins in both zebrafish and humans. Overall, spirotetramat exerts significant neurotoxic effects in zebrafish, which may be associated with disruption of neurotransmitter homeostasis and visual-related signaling pathways. These findings provide new insights into the neurotoxic potential of spirotetramat and provide a basis for future experimental investigation of its ecological and potential human health risks.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Ziyang Lai,Mengchang He
Owing to the extensive application of antimony across various industries, antimony pollution poses significant threats to human health and ecosystems. However, its aquatic ecotoxicological and mechanisms remain poorly understood. This study aimed to decipher the toxic effects and molecular mechanisms of antimony(V) [Sb(V)] in zebrafish embryos/larvae. The results demonstrated that Sb(V) accumulated in larvae in a concentration-dependent manner. Although no significant lethal or teratogenic effects were observed, normal growth and development were perturbed. Exposure to 100 mg/L Sb(V) increased reactive oxygen species (ROS) levels in larvae while increasing catalase (CAT) and superoxide dismutase (SOD) activities and reducing lipid peroxidation levels, with no significant effect on apoptosis. Correlation analysis of toxic endpoints revealed complex network associations among the antioxidant system (CAT/SOD), oxidative stress markers (ROS/MDA), and developmental parameters (body length, heart rate, and nonteratogenic rate). The antioxidant functions of CAT/SOD may indirectly influence growth and teratogenic phenotypes by modulating the extent of oxidative damage, whereas the glutathione redox balance (GSH/GSSG ratio) has bidirectional regulatory potential for apoptosis. Oxidative stress indicators (CAT, SOD, ROS, and MDA), apoptosis markers (whole-body and abdominal apoptosis), and developmental metrics were identified as highly sensitive toxic endpoints. Transcriptomic analysis revealed that Sb(V) upregulated immune-related signaling pathways and significantly affected the expression of genes involved in immune defense responses in zebrafish larvae, suggesting potential interference with immune system functions. This study provides critical data for enhancing the understanding of Sb(V) aquatic ecotoxicology and offers new perspectives for assessing the potential ecological health risks of Sb(V).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Chanlin Fang,Liya Fang,Shanshan Di,Yundong Yu,Xinquan Wang,Caihong Wang,Yuanxiang Jin
N-(1,3-dimethylbutyl)- N ′-phenyl- p -phenylenediamine (6PPD) is a type of p -phenylenediamine (PPD), which is widely used in the manufacture of rubber tires owing to its excellent antiozonant properties. In this study, the developmental cardiotoxicity of 6PPD was evaluated in zebrafish larvae, and the LC50 was approximately 737 μg/L for the larvae at 96 h post fertilization (hpf). In the 6PPD treatment of 100 μg/L, the accumulation concentrations of 6PPD were up to 2658 ng/g in zebrafish larvae, and 6PPD induced significant oxidative stress and cell apoptosis in the early developmental stages of zebrafish. Transcriptome analysis showed that 6PPD exposure could potentially cause cardiotoxicity in larval zebrafish by affecting the transcription of the genes related to the calcium signal pathway and cardiac muscle contraction. The genes related to calcium signaling pathway ( slc8a2b , cacna1ab , cacna1da , and pln ) were verified by qRT-PCR, which were significantly downregulated in larval zebrafish after exposing to 100 μg/L of 6PPD. Simultaneously, the mRNA levels of the genes related to cardiac functions ( myl7 , sox9 , bmp10 , and myh71 ) also respond accordingly. H&E staining and heart morphology investigation indicated that cardiac malformation occurred in zebrafish larvae exposed to 100 μg/L of 6PPD. Furthermore, the phenotypic observation of transgenic Tg (myl7: EGFP) zebrafish also confirmed that 100 μg/L of 6PPD exposure could change the distance of atria and ventricles of the heart and inhibit some key genes ( cacnb3a , ATP2a1l , ryr1b ) related to cardiac function in larval zebrafish. These results revealed the toxic effects of 6PPD on the cardiac system of zebrafish larvae.
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Yumiao Zhou,Qiang Kong,Zhihao Lin,Jinyue Ma,Huanxin Zhang
Waterborne Benzo[a]pyrene (B[a]P) pollution is a global threat to aquatic organisms. The exposure to waterborne B[a]P can disrupt the normal locomotor behavior of zebrafish ( Danio rerio ), however, how it affect the locomotor behavior of adult zebrafish remains unclear. Herein, B[a]P at two concentrations (0.8 μg/L and 2.0 μg/L) were selected to investigate the molecular mechanisms of the affected locomotor behavior of zebrafish by B[a]P based on transcriptome profiling. Adverse effects of B[a]P exposure affecting locomotor behavior in zebrafish were studied by RNA sequencing, and the locomotion phenotype was acquired. The gene enrichment results showed that the differentially highly expressed genes ( atp2a1, cdh2, aurka, fxyd1, clstn1, apoc1, mt-co1, tnnt3b, and fads2 ) of zebrafish are mainly enriched in adrenergic signaling in cardiomyocytes (dre04261) and locomotory behavior (GO:0007626). The movement trajectory plots showed an increase in the locomotor distance and velocity of zebrafish in the 0.8 μg/L group and the opposite in the 2.0 μg/L group. The results showed that B[a]P affects the variety of genes in zebrafish, including motor nerves, muscles, and energy supply, and ultimately leads to altered locomotor behavior.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2020
·Vol. 35
·期 10
·1091-1099
·
IF 3.118
Dantong Jia,Xiuwen Li,Sen Du,Ning Xu,Wenming Zhang,Ruyi Yang,Yunhai Zhang,Yide He,Yongjun Zhang
Various pollutants co-exist in the aquatic environment such as carbamazepine (CBZ) and copper (Cu), which can cause complex effects on inhabiting organisms. The toxic impacts of the single substance have been studied extensively. However, the studies about their combined adverse impacts are not enough. In the present study, zebrafish were exposed to environmental relevant concentrations of CBZ (1, 10, and 100 μg/L), Cu (0.5, 5, and 10 μg/L) and the mixtures (1 μg/L CBZ + 0.5 μg/L Cu, 10 μg/L CBZ + 5 μg/L Cu, 100 μg/L CBZ + 10 μg/L Cu) for 45 days, the effects on nervous and antioxidant systems of zebrafish were investigated. The results demonstrated that, in comparison with single exposure group, the combined presence of CBZ and Cu exacerbated the effect of antioxidant system (the ability of inhibition of hydroxyl radicals (IHR), superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST)) but not nervous system (Acetylcholinesterase [AChE]). The qPCR results supported the changes of corresponding enzymes activities. Hepatic histopathological analysis verified the results of biomarkers. Our work illustrated that the toxicity of mixed pollutants is very complicated, which cannot simply be inferred from the toxicity of single pollutant, and calls for more co-exposure experiments to better understanding of the co-effects of pollutants on aquatic organisms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2020
·
IF 6.551
Wenhui Qiu,Xinjie Liu,Feng Yang,Rongzhen Li,Ying Xiong,Caixia Fu,Guanrong Li,Shuai Liu,Chunmiao Zheng
In China, antibiotics are commonly used for human and veterinary medicine, and they are present in various environmental media. Thus, the toxic effects of antibiotics on organisms have attracted the attention of society and scientists alike. In this study, zebrafish embryos were used to test the single and joint toxicity of four antibiotics, sulfamonomethoxine (SMM), cefotaxime sodium (CFT), tetracycline (TC), enrofloxacin (ENR), and their combinations, combining the results of experimental and omics techniques. Following exposure to antibiotics for 120 h, the body lengths of zebrafish larvae in all 100 μg/L antibiotic groups were significantly shortened, and the reactive oxygen species (ROS) content in the 100 μg/L Mix group was significantly increased. Transcriptome sequencing (RNA-seq) showed that the mRNA level of numerous genes was significantly changed in the five antibiotic treatment groups. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the differentially expressed genes revealed a significant enrichment of the steroid biosynthesis and other metabolism pathways. Hub gene analysis highlighted dhcr24 , acat1 , aldh1a2 , aldh8a1 , suclg2 , hadh , and hsdl2 as the key genes, and hub gene expression changes because of the antibiotic treatment suggested that the metabolic system of the zebrafish larvae was severely disrupted by the interaction with other genes. In conclusion, single or joint exposure to different antibiotics at environmental concentrations affected the early development and metabolic system of zebrafish larvae, and our results provide fundamental evidence for future studies of antibiotic toxicity in aquatic organisms.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Yiyun Liu,Yan-Ling Liao,Jin-Xia Wang,Bao-Fu Zhang,Qi-Ping Tang,Yuan Ding,Si-Jia Zuo,Qin-Hong Zhou,De-Sheng Pei
The widespread application of nano-cerium dioxide (Nano-CeO 2 ) in agriculture, industry, and other fields has led to an increasing amount of it being released into the environment and becoming a pollutant with potential biological hazards. However, studies on the effects of ceria on cardiovascular development were limited. This study aims to detect the cardiovascular toxicity of Nano-CeO 2 , focusing on its impact on zebrafish hematopoietic function and cardiac development, and to explore its potential molecular mechanisms. We found that exposing zebrafish embryos to different concentrations of Nano-CeO 2 after 6 hpf of fertilization does not hinder the growth and development of zebrafish embryos during their early life stages. However, RNA-Seq analysis indicates that the hematopoietic system of zebrafish is suppressed, the ECM-receptor interaction pathway is disrupted, and the expression of related genes is significantly downregulated. The results of o-dianisidine staining, neutral red staining, and Sudan black staining showed that the hemoglobin, macrophages, and neutrophils of zebrafish were reduced, and the vascular dysplasia of zebrafish was observed. Meanwhile, Reactive oxygen species (ROS) accumulated in the heart of zebrafish larvae treated with Nano-CeO 2 , and heart development-related genes ( nppa , hand2 , nppb , nkx2.5 , and myh7 ) were significantly down-regulated, while gata4 was up-regulated. The addition of the integrin agonist manganese ions restored the expression of genes related to ECM-receptor interaction pathways, alleviating cardiovascular abnormalities caused by Nano-CeO 2 . Besides, significant changes in cardiac histopathology were observed with long-term exposure (120 d post-fertilization), indicating that Nano-CeO 2 exposure affected the development of the cardiovascular system in zebrafish. In summary, acute and chronic exposure to Nano-CeO 2 exhibits cardiovascular toxicity in zebrafish, affecting hematopoietic function and cardiac development, and the mechanism may be related to the ECM-receptor interaction pathway. This study provides useful information for a comprehensive understanding of the toxicity mechanisms and environmental risks of Nano-CeO 2 .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 20
·16790-16797
·
IF 3.289
Qian Zhao,Lin Zhu
Humic acid (HA) is the main component of dissolved organic matter in aquatic ecosystems and affects the bioavailability of contaminants. Prometryn is frequently used to control annual broadleaf and grass weeds and has been widely detected in the aquatic environment. An experiment was conducted to investigate the effect of HA on the chronic toxicity of prometryn in zebrafish. In zebrafish treated with 53.2 μg L−1 prometryn (P), prometryn with 5 mg L−1 HA (P + HA5), or prometryn with 15 mg L−1 HA (P + HA15), catalase (CAT) activity and glutathione (GSH) content in the visceral mass initially increased and then decreased with exposure time. After one day of exposure, malondialdehyde in the visceral mass increased by 58% to 64% in fish treated with prometryn and HA compared to those treated with prometryn alone, however, this difference disappeared during days 10 to 40. Treatment with HA enhanced the bioaccumulation of prometryn by 34% and 40% on day 1 in the P + HA5 and P + HA15 groups, respectively, possibly due to changes in gill membrane permeability. Nevertheless, the opposite result was observed during days 10 to 40, owing to the presence of more excreta and suspended particulate matter resulting in a reduction in the quantity of free dissolved prometryn in the P + HA5 and P + HA15 groups. There were significant correlations between CAT activity and prometryn concentration (0.562**, P < 0.01) and between GSH and prometryn concentration (0.808**, P < 0.01) throughout the first 20 days of the experiment, suggesting a role for these antioxidant systems in the detoxification of prometryn in zebrafish.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）