SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Hongping Liao,Dandan Gao,Muhammad Junaid,Shulin Liu,Chunmiao Kong,Xikun Chen,Ting Pan,Qingzhi Zheng,Wenjie Ai,Guanglong Chen,Jun Wang
The ubiquitous presence of polystyrene nanoplastics (PSNPs) and di(2-ethylhexyl) phthalate (DEHP) in the aquatic environment may cause unpredictable negative effects on aquatic organisms and even continue to the offspring. This study assessed the transgenerational impacts of parental exposure to PSNPs and DEHP over four generations (F0–F3) of Daphnia magna . A total of 480 D. magna larvae (F0, 24 h old) were divided into four groups with six replicates (each of them contains 20 D. magna ) and exposed with dechlorinated tap water (control), 1 mg/L PSNPs, 1 μg/L DEHP, and 1 mg/L PSNPs + 1 μg/L DEHP (PSNPs-DEHP) until spawning begins. Subsequent to exposure, all the surviving F1 offspring were transferred to new water and continued to be cultured until the end of F3 generation births in all groups. The results showed that the PSNPs accumulated in F0 generation and were inherited into F1 and F2 generations, and disappeared in F3 generation in PSNPs and PSNPs-DEHP groups. However, the accumulation of DEHP lasted from F0 generation to F3 generation, despite a significant decline in F2 and F3 generations in DEHP and PSNPs-DEHP groups. The accumulation of PSNPs and DEHP caused overproduction of reactive oxygen species in F0–F2 generations and fat deposition in F0–F3 generations. Additionally, single and in combination parental exposure to PSNPs and DEHP induced regulation of growth-related genes ( cyp18a1 , cut , sod and cht3 ) and reproduction-related genes ( hr3 , ftz-f1 , vtg and ecr ) in F0–F3 generations. Survival rates were decreased in F0–F1 generations and recovered in F2 generation in all treatment groups. Furthermore, the spawning time was prolonged and the average number of offspring was increased in F1–F2 generaions as a defense mechanism against population mortality. This study fosters a greater comprehension of the transgenerational and reproductive effects and associated molecular mechanisms in D. magna .
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高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 10
·9636-9646
·
IF 2.8
Li Chenguang,Qu Ruijuan,Chen Jing,Zhang Shuo,Allam Ahmed A.,Ajarem Jamaan,Wang Zunyao
Triclosan (TCS) is an antibacterial and antifungal agent widely used in personal care products, and it has been frequently detected in the aquatic environment. In the present study, the acute toxicity of TCS to Daphnia magna , Photobacterium phosphoreum , Danio rerio , and Limnodrilus hoffmeisteri was assessed under different pH conditions. Generally, TCS was more toxic to the four aquatic organisms in acidic medium. The LC 50 values for D. magna and D. rerio were smaller among the selected species, suggesting that D. magna and D. rerio were more sensitive to TCS. In addition, the oxidative stress-inducing potential of TCS was evaluated in Carassius auratus at three pH values. Changes of superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH) level, and malondialdehyde (MDA) content were commonly observed in all TCS exposure groups, indicating the occurrence of oxidative stress in the liver of C. auratus . The integrated biomarker response (IBR) index revealed that a high concentration of TCS induced great oxidative stress in goldfish under acidic condition. This work supplements the presently available data on the toxicity data of TCS, which would provide some useful information for the environmental risk assessment of this compound.
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ENVIRONMENTAL POLLUTION
·
2020
·
IF 6.793
Cen Yin,Xianhai Yang,Tianyi Zhao,Peter Watson,Feifei Yang,Huihui Liu
The effects of microplastics on aquatic organisms are drawing growing attention, but little has been focused on their effects on the toxicity of other chemicals. In this study, we examined the acute and chronic toxicity of micro-polystyrene (5.8 μm dia.), and its effects on the toxicity of three antimicrobial agents (triclosan, triclocarban and methyl-triclosan) to Daphnia magna . Results indicated that polystyrene had a low acute toxicity with an EC 50 of 36.5 mg/L. The presence of polystyrene (1 mg/L) did not produce significant effect on the acute toxicity of three chemicals, because the 95% confidence intervals of their EC 50 values had a large overlap of 11.3%–48.3%. For the 21 day chronic toxicity, polystyrene alone had significant toxicity with concentrations of at least 2 mg/L, which prolonged the time of the first brood, limited the number of broods, and reduced the total number of neonates. Compared with the chemicals alone, the addition of polystyrene enhanced their reproduction toxicity. Based on the various reproduction indicators, an intrinsic rate of natural increase ( r m ) was calculated to assess the rate of population growth. Results suggested that the r m values of three chemicals decreased in the presence of PS, and further decreased with increasing PS concentrations. Among the three chemicals, methyl-triclosan was the most affected. These results suggested that the presence of microplastics would exacerbate the detrimental influence of pollutants on Daphnia magna .
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高影响因子
ECOTOXICOLOGY
·
2024
·
IF 2.4
Yang Liu,Liu Kexin,Shi Lina,Chen Ming,Liu Junyan,Dai Shiniu,Xi Yilong,Wen Xinli
N-(1,3-dimethylbutyl)-N’-phenyl-p-phenylenediamine (6PPD) and Zn 2+ , extensively used in the tire manufacturing process, are frequently detected in freshwater environments. However, the intergenerational effects of isolated 6PPD exposure and joint 6PPD and Zn 2+ exposure at concentrations approximating environmental levels remain unknown. This study assessed the chronic toxicity and intergenerational effects of 6PPD (0.02–20 μg/L) and a mixture of 6PPD and Zn 2+ (5 μg/L) over three generations in Daphnia magna bioassays. In the F0 generation, a dose-dependent decline in total offspring number was observed with 6PPD exposure alone, while co-exposure with Zn 2+ exacerbated the reproductive toxicity of 6PPD. Across three generations, low-dose (0.02 µg/L) 6PPD alone and combined with Zn 2+ induced a cumulative degenerative maternal effect. Conversely, high-dose (20 µg/L) 6PPD, both independently and in combination with Zn 2+ , exhibited an adaptive maternal effect. Notably, the grandmaternal effect emerged exclusively in the co-exposure group treated with 20 μg/L 6PPD and 5 μg/L Zn 2+ , with no such effect in the group exposed to 20 μg/L 6PPD alone, suggesting that Zn 2+ may enhance the potential toxicity of 6PPD. Overall, this study provides novel insight into the intergenerational impacts of environmentally relevant levels of 6PPD alone and in combination with a heavy metal, elucidating the environmental risks posed by tire-derived chemicals through their synergistic effects on transgenerational toxicity.
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高影响因子
Toxics
·
2026
·Vol. 14
·期 10
·862
·
IF 4.9
Basit Ali Khan,Minh-Quan Tran,Muhammad Baqir Khan,Chung-Der Hsiao
Heavy metals (HMs) and rare earth elements (REEs) persist as environmental contaminants that accumulate in aquatic ecosystems and pose risks to aquatic organisms. Although the toxicity of HMs is well-established, the ecological and biological impacts of REEs remain comparatively underexplored, despite their expanding industrial use. In this study, zebrafish (Danio rerio) larvae andDaphnia magnaneonates were employed as complementary aquatic models to comparatively assess the toxicological effects of multiple metal groups, leveraging their rapid development and suitability for high-throughput behavioral analysis. Both species were exposed to 17 HMs and 16 REEs across concentration ranges defined by species-specific guidelines to determine LC50values, followed by 24 h behavioral assessments at sublethal concentrations, and employing optimized, high-throughput protocols. Thirteen behavioral endpoints were analyzed to comprehensively characterize locomotor activity, spatial exploration, and movement complexity. Multivariate behavioral patterns were evaluated using principal component analysis and heatmap clustering. The results revealed pronounced species- and metal-specific toxicity profiles, with several REEs inducing behavioral effects comparable to or exceeding those of certain HMs. Notably, behavioral responses differed between zebrafish larvae andDaphnia magnafor the same compounds, highlighting species-specific sensitivity and underscoring the value of multi-species assessment for environmental risk evaluation.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2023
·
IF 13.6
Hongping Liao,Dandan Gao,Chunmiao Kong,Muhammad Junaid,Ye Li,Xikun Chen,Qingzhi Zheng,Guanglong Chen,Jun Wang
Nanoplastics and di(2-ethylhexyl) phthalate (DEHP) are ubiquitous emerging contaminants that are transferred among organisms through food chain in the ecosystem. This study evaluated the trophic transfer of polystyrene nanoplastics (PSNPs) and DEHP in a food chain including Chlorella pyrenoidosa , Daphnia magna and Micropterus salmoides (algae-crustacean-fish) and lipid metabolism at a higher trophic level in fish. Our results showed that the PSNPs and DEHP accumulated in C. pyrenoidosa or D. magna were transferred to the M. salmoides , of which the DEHP were not biomagnified, while the PSNPs were trophically amplified by the food chain. It is suggested that more PSNPs might be accumulated by higher level consumers in a longer food chain. Additionally, the trophic transfer of PSNPs and DEHP resulted in antioxidant response and histopathological damage in M. salmoides . Moreover, the lipid biochemical parameters and lipid metabolism related genes ( fasn , hsl , cpt1a , atgl , apob , fabp1 , lpl , cetp ) of M. salmoides were significantly affected, which indicated disturbance of lipid metabolism. This study offers great insight into the transfer of contaminants by trophic transfer and their negative effects on organisms at higher trophic levels, which cause human exposure to MNPs and organic contaminants in the ecosystem.
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高影响因子
AQUATIC TOXICOLOGY
·
2025
·
IF 4.3
Liu Yang,Xue Tang,Yongsheng Zhou,Shiniu Dai,Kexin Liu,Lina Shi,Xinli Wen
A common tire additive of N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) is frequently detected in freshwater. To date, the toxicity of 6PPD to aquatic organisms has mainly been based on partial life-cycle test results. However, it remains unclear whether this approach leads to an underestimation of the true environmental risk of 6PPD. Here, the effects of environmentally relevant or greater concentrations of 6PPD on the reproduction and growth of Daphnia magna were specifically compared between day 21 and the day all the maternal daphnia died, with the underlying toxic mechanisms of 6PPD also explored. 6PPD at environmental or near-natural water concentrations (5.68 and/or 9.20 µg/L) significantly decreased the total number of offspring and net reproductive rate after whole-life-stage exposure. However, such adverse effects were not observed on day 21, indicating that environmental levels of 6PPD exerted reproductive toxicity on D. magna , and short-term exposure may underestimate the environmental risk of 6PPD. Elevated malondialdehyde levels in the 5.68, 9.20 and 44.58 µg/L treatment groups, coupled with reactive oxygen species (ROS) emerging as the dominant contributors to the biomarker response index, indicated that ROS overproduction induced by 6PPD triggered lipid peroxidation despite antioxidant capacity was enhanced. Downregulation of the vitellogenin expression in D. magna exposed to 5.68, 9.2 and 44.58 µg/L 6PPD, likely resulting from competitive binding to ecdysteroid receptor based on molecular docking and molecular dynamics simulation, might compromise nutrient provisioning during embryogenesis. Coupled with oxidative stress, these dual mechanisms appeared to mediate 6PPD-induced reproductive toxicity.
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高影响因子
危险品化学品经营许可证（带存储）