INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Ju Liu,Danna Yan,Qianying Chen,Huayong Peng,Lu Jin,Chong Feng,Lixia Zhang,Guomin Zhao,Depo Yang,Zhimin Zhao
In this study, a homogeneous polysaccharide, named YDOP-1 was isolated form Dendrobium officinale using hot water extraction and ethanol precipitation method. YDOP-1 was characterized to be a typical O -acetyl-glucomannan with the molecular wight was 13,456 Da. Cell viability and colony forming assay showed that YDOP-1 possess moderate anti-gastric cancer effects. In order to further improve the anti-gastric cancer effects of YDOP-1, a selenium modification on YDOP-1 was performed. Energy dispersive spectrometer (EDS) and X-ray photoelectron spectroscopy (XPS) analysis demonstrated that the Se was successfully introduced into YDOP-1 by forming Se O bonds. Se modification significantly enhanced the anti-gastric cancer effects of YDOP-1, which could effectively inhibit the proliferation of MGC-803 cells via suppressing colony and inducing apoptosis by regulating the apoptosis proteins of Bax, Bcl-2, and Caspase-7. Our results indicated that the Se modified O -acetyl-glucomannan, YDOP-Se, was expected to be useful in the biomedicine field for the gastric cancer treatment.
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Food Bioscience
·
2024
·
IF 4.8
Luo Xiaoye,Wanlin Liu,Wu Huang,Xiaodan Wang,Linling Li,Xin Zhao,Shuyi Qiu
In this study, Saccharomycopsis fibuligera FBKL2.8DCJS1 and Lacticaseibacillus paracasei FBKL1.3028 were jointly introduced into Dendrobium officinale liquid to probe alterations in their active constituents. Over a 15-day fermentation period, analyses were conducted to ascertain shifts in physicochemical attributes, antioxidant capacity, metabolite transformation, flavor profiles, and sensory characteristics. The findings revealed augmented color differentiation, increased antioxidant potential, and heightened acidity post-fermentation. This finding points to a positive correlation between acidity and color variation and antioxidant capacity. The microbial fermentation process was advantageous for ameliorating both the color and taste of D. officinale while also bolstering its antioxidant capacity. Additionally, several bioactive substances favorable to the organism, including metabolites such as proscillaridin, D-sorbitol, and glucomannan, were found to be upregulated postfermentation. The most enriched metabolic pathways identified in the KEGG metabolic pathway were purine metabolism and amino acid metabolism. Notably, co-cultivating S. fibuligera and L. paracasei amplified the production of ethyl lactate, indicating that this co-culture approach induced higher levels of this compound. Consequently, fermenting D. officinale with S. fibuligera and L. paracasei not only enhanced its bioactive properties but also enhanced its flavor profile.
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Food Bioscience
·
2024
·
IF 4.8
Zhexuan Xu,Zhihan Zheng,Gonglin Cai,Bo Chen,Hailong Yang
Fermentation is a technique commonly used to improve the health of agricultural products. In this study, Dendrobium officinale leaves were solid-state fermented by edible fungi, and the results showed that Aspergillus oryzae was the suitable strain to increase their inhibition abilities against α-glucosidase and α-amylase by 9.05- and 2.99-times, respectively. Chemical analysis showed that the contents of total phenolics, free phenolics, bound phenolics, quercetin, and rhoifolin were increased by 2.54-, 3.41-, 2.09-, 6.03-, and 8.06-times, while those of luteolin, gallic acid, and kaempferol were increased from undetectable level to 0.45, 46.99, and 4.47 μg/g, respectively, after four days of fermentation by A. oryzae . Furthermore, the hypoglycemic activity of D. officinale leaves was tested in vivo . The fermented group showed higher capacity for decreasing fasting blood glucose levels and insulin resistance, reversing dyslipidemia, ameliorating oxidative stress, and reversing pancreatic and liver lesions in db/db mice. These findings suggest that it is effective to utilize and valorize D. officinale leaves as functional food or food additives through the fermentation by A. oryzae .
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MATERIALS & DESIGN
·
2026
·
IF 8.2
Yang Yang,Jingjian Liu,Jiye Zhang,Shuya Zhang,Jiazhen Wu,Qizhang Wang,Guangyi Yang,Qinhua Chen
Refractory diabetic wound is characterized by excessive oxidative stress and inadequate angiogenesis, both of which are primarily caused by mitochondrial dysfunction. Although nanozymes exhibit multiple enzyme-mimicking antioxidant activities, challenges such as immune rejection, limited catalytic activity, and poor biocompatibility remain. Here in, a novel therapeutic system based on plant exosome nanovesicle-incorporated nanozymes was designed to mitigate oxidative stress and promote healing in diabetic wounds by targeting and restoring mitochondrial function. Titanium-doped cerium vanadate (CeVO 4 -Ti) nanozymes with multiple enzyme catalytic activities had been rationally designed. The surface of CeVO 4 -Ti was modified with triphenylphosphine (TPP) to endow mitochondrial targeting. To enhance biosafety and delivery, the nanozymes were further encapsulated within dendrobium officinale-derived nanovesicles (DOEVs) and integrated into a dopamine-methacrylated hyaluronic acid (DHM) hydrogel, forming a composite system (DOEVs@CTT/DHM). DOEVs@CTT/DHM could effectively mimic the activity of natural enzymes, eliminate reactive oxygen species, reconstruct blood vessels, and promote re-epithelialization, thereby facilitating wound healing from multiple perspectives. Crucially, it activated the Nrf2/Parkin/Pink1 pathway to regulate mitophagy, thereby revitalizing mitochondrial function and then alleviating oxidative stress. This study presents a novel strategy for mitochondrial regulation, advancing the development of multifunctional nanozyme-based biomaterials for wound healing.
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FOOD HYDROCOLLOIDS
·
2022
·
IF 11.504
Hua Zhu,Lei Xu,Jian Wang,Zhihan Zhang,Xinhui Xu,Kai Yang,Peilong Sun,Xiaojun Liao,Ming Cai
Ethanol fractional precipitation is an important technology to classify polysaccharides at different molecular weights. In this study, polysaccharides of Dendrobium officinale were step-precipitated by final ethanol concentrations of 40%, 60% and 80%, named as EPDO-40, EPDO-60 and EPDO-80, respectively. Chemical composition, molecule weights and monosaccharides were identified. Effects of concentration, temperature, pH and metal ions on rheological properties were investigated. Results showed that all EPDOs behaved as non-Newtonian fluid in a certain shear rate range of 0.1–1000 s −1 which is affected by their different chemical characteristics. Viscosities of all polysaccharides showed a concentration dependent manner, and they decreased with the increased temperatures from 10 to 90 °C. Viscosities of EPDO-40 and EPDO-80 increased at a range of lower pH significantly, while that of EPDO-60 decreased. Na + , K + , Mg 2+ and Ca 2+ could all affect the viscosity of EPDOs but in different patterns. Accordingly, it demonstrated that EPDOs with different chemical characteristics had different rheological properties, which could be applied more accurately in foods industry.
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Antioxidants
·
2023
·Vol. 12
·期 11
·1911
·
IF 7
Gonglin Cai,Hangmeng Dong,Shoulong Liu,Weijie Wu,Hailong Yang
Dendrobium officinaleleaves have the potential to be processed into natural antioxidants, functional foods, and food additives. To maximally maintain their quality, freshD. officinaleleaves were dehydrated using different drying methods, i.e., hot air drying (HD), microwave drying (MD), infrared drying (IRD), and freeze drying (FD), and then the physicochemical properties, microstructure, and biological activities of the dried samples were compared. The results showed that, with the FD method, the samples had a porous microstructure, maintained the highest phenolic content, and demonstrated the highest antioxidant and hypoglycemic activities. Among the three thermal drying methods, with the IRD method, the samples retained higher phenolic contents, showed stronger DPPH free-radical scavenging, ferric ion reducing, ferrous ion chelating, and α-glucosidase inhibitory abilities, and more strongly promoted glucose metabolism in insulin-resistant HL-7702 cells than the samples with the MD and HD methods. These results suggested that FD was the most suitable method. However, IRD might be a promising alternative, owing to the high cost and long time needed for FD for the large-scale drying ofD. officinaleleaves.Keywords:Dendrobium officinaleleaves;drying methods;antioxidant activity;hypoglycemic activity;phenolic compounds
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Foods
·
2024
·Vol. 13
·期 11
·1675
·
IF 4.7
Lei Xu,Hua Zhu,Peng Chen,Zhenhao Li,Kai Yang,Peilong Sun,Fangting Gu,Jianyong Wu,Ming Cai
Polysaccharides fromDendrobium officinalehave garnered attention for their diverse and well-documented biological activities. In this study, we isolated three ethanol-fractionated polysaccharides fromDendrobium officinale(EPDO) and investigated their digestive properties and effects on gut microbiota regulation in vitro. The results indicated that after simulating digestion in saliva, gastric, and small intestinal fluids, three EPDOs, EPDO-40, EPDO-60 and EPDO-80, with molecular weights (Mw) of 442.6, 268.3 and 50.8 kDa, respectively, could reach the large intestine with a retention rate exceeding 95%. During in vitro fermentation, the EPDOs were broken down in a “melting” manner, resulting in a decrease in theirMw. EPDO-60 degraded more rapidly than EPDO-40, likely due to its moderateMw. After 24 h, the total production of short-chain fatty acids (SCFAs) for EPDO-60 reached 51.2 ± 1.9 mmol/L, which was higher than that of EPDO-80. Additionally, there was an increase in the relative abundance ofBacteroides, which are capable of metabolizing polysaccharides. EPDO-60 also promoted the growth of specific microbiota, includingPrevotella9 andParabacteroides, which could potentially benefit from these polysaccharides. Most notably, by comparing the gut microbiota produced by different fermentation carbon sources, we identified the eight most differential gut microbiota specialized in polysaccharide metabolism at the genus level. Functional prediction of these eight differential genera suggested roles in controlling replication and repair, regulating metabolism, and managing genetic information transmission. This provides a new reference for elucidating the specific mechanisms by which EPDOs influence the human body. These findings offer new evidence to explain how EPDOs differ in their digestive properties and contribute to the establishment of a healthy gut microbiota environment in the human body.
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Food Chemistry-X
·
2022
·
IF 6.443
Jingyu Fang,Yang Lin,Hualing Xie,Mohamed A. Farag,Simin Feng,Jinjun Li,Ping Shao
The present study aimed to explore the possible mechanisms underlying Dendrobium officinale leaf polysaccharides of different molecular weight to alleviate glycolipid metabolic abnormalities, organ dysfunction and gut microbiota dysbiosis of T2D mice. An ultrafiltration membrane was employed to separate two fractions from Dendrobium officinale leaf polysaccharide named LDOP-A and LDOP-B. Here, we present data supporting that oral administration of LDOP-A and LDOP-B ameliorated hyperglycemia, inhibited insulin resistance, reduced lipid concentration, improved β-cell function. LDOP-A with lower molecular weight exhibited improved effect on diabetes than LDOP-B, concurrent with increased levels of colonic short-chain fatty acids (SCFAs) i.e., butyrate, decreased ratio of Firmicutes to Bacteroidetes phyla, and increased abundance of the gut beneficial bacteria i.e., Lactobacillus , Bifidobacterium and Akkermansia . These results suggest that LDOP-A possesses a stronger effect in ameliorating T2D than LDOP-B which may be related to the distinct improved SCFAs levels produced by the change of intestinal flora microstructure.
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MOLECULES
·
2020
·Vol. 25
·期 3
·759
·
IF 3.267
Bo Wang,Haiyan Tian,Dong Xiang
Coconut oil-in-water emulsions were prepared using three polysaccharides:Dendrobium officinalepolysaccharide (DOP), propylene glycol alginate (PGA), gum arabic (GA) and their polysaccharide complexes as emulsifiers. The effects of the ratio of the compounded polysaccharides on their apparent viscosity and interfacial activity were explored in this study. The average particle size, zeta potential, microstructure, rheological properties, and physical stability of the emulsions prepared with different compound-polysaccharides were studied. The results showed that mainly DOP contributed to the apparent viscosity of the compound-polysaccharide, while the interfacial activity and zeta potential were mainly influenced by PGA or GA. Emulsions prepared with compound-polysaccharides exhibited smaller average particle sizes, and microscopic observations showed smaller droplets and less droplet aggregation. In addition, the stability analysis of emulsions by a dispersion analyzer LUMiSizer showed that the emulsion prepared by compounding polysaccharides had better physical stability. Finally, all of the above experimental results showed that the emulsions prepared by PGA:DOP = 2:8 (total concentration = 1.5 wt%) and 2.0% GA + 1.5% DOP were the most stable.Keywords:Dendrobium officinalepolysaccharides;propylene glycol alginate;gum arabic;coconut oil-in-water emulsion
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Han Yue,Yanqiu Liu,Huanhuan Qu,Kan Ding
Dendrobium officinale Kimura et Migo (Tie-Pi-Shi-Hu), a precious folk medicine exhibiting multiple bioactivities, including antitumor, immune-enhancing and so on. Although evidences showed polysaccharide is one of the major bioactive substances from this herb, several of them were homogenous with fine structures elucidated. In this study, we showed a novel homogeneous heteroxylan obtained from alkali-extracted crude polysaccharide. It composed of arabinose, xylose, glucose and 4- O -methylglucuronic acid (4-MGA) as well as trace amount of rhamnose and galactose in a ratio of 8.9:62.7:8.5:12.3:3.9:3.7. We further showed that it contained a backbone of 1,4-linked β- d -xylan, with branches of 1,4-linked α- d -glucose, 1,3-linked α- l -rhamnose, and terminal-linked α- l -arabinose, β- d -galactose, 4-MGA, and β- d -xylose directly or indirectly attached to C-2 position of glycosyl residues on backbone. The sulfated derivative with substitution degree about 0.9 was prepared according to the chlorosulfonic acid (CSA)-pyridine method. Bioactivity tests suggested that the sulfated polysaccharide could significantly disrupt tube formation and inhibit the migration of human microvascular endothelial cells (HMEC-1) at a low concentration (0.29 μM) in a dose-dependent way without significant cytotoxity.
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INDUSTRIAL CROPS AND PRODUCTS
·
2026
·
IF 6.4
Nan Wang,Ge Chen,Zhenyun Li,Qizhao Xia,Jie Xin,Chi Zhang
D. officinale cellulose was extracted and oxidized into nanofibrils. • CNFs and zein formed nanostructured W/O/W Pickering delivery systems. • Higher oil fractions enhanced emulsion viscoelasticity and gel networks. • The delivery nanosystems protected Sal B for targeted oral delivery. This study developed novel W/O/W Pickering double emulsions (PDEs) stabilized by Dendrobium officinale Kimura et Migo-derived cellulose nanofibrils (CNFs) and zein nanoparticles (ZNPs) for the delivery of salvianolic acid B (Sal B). CNFs were obtained via TEMPO oxidation, forming a highly extended, interwoven nanoscale network. Driven by electrostatic, hydrogen-bonding, and hydrophobic interactions, CNFs and ZNPs (1:2) co-assembled at pH 4.0. This assembly enabled the one-step fabrication of W/O/W PDEs, as confirmed by optical and confocal laser scanning microscopy. The emulsion droplets remained uniformly distributed and highly stable over a 30-day storage period. Rheological analyses showed that increasing the oil fraction from 50% to 70% induced pronounced elastic gel behavior and enhanced system viscoelasticity. These higher lipid fractions effectively reduced free fatty acid release during simulated in vitro gastrointestinal digestion. More importantly, the construct successfully protected Sal B from premature degradation, enabling its release in the gut. These results highlight the potential of D. officinale cellulose as a sustainable plant stabilizer and establish a nanocellulose-based formulation platform for plant bioactive compounds in pharmaceutical and industrial applications. Download: Download high-res image (236KB) Download: Download full-size image
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Frontiers in Immunology
·
2023
·
IF 7.3
Zhou Huiji,Zhou Luxian,Li Bo,Yue Rongcai
Introduction: Dendrobium officinale Kimura et Migo (D. officinale) , widely called as “life-saving immortal grass” by Chinese folk, is a scarce and endangered species. The edible stems of D. officinale have been extensively studied for active chemical components and various bioactivities. However, few studies have reported the well-being beneficial effects of D. officinale flowers (DOF). Therefore, the present study aimed to investigate the in vitro biological potency of its aqueous extract and screen its active components.Methods: Antioxidant tests, including 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), the ferric reducing ability of plasma (FRAP), and intracellular reactive oxygen species (ROS) level analyses in primary human epidermal keratinocytes, anti-cyclooxygenase2 (COX-2) assay, anti-glycation assay (both fluorescent AGEs formation in a BSA fructose/glucose system and glycation cell assay), and anti-aging assay (quantification of collagen types I and III, and SA-β-gal staining assay) were conducted to determine the potential biological effects of DOF extracts and its major compounds. Ultra-performance liquid chromatography-electrospray ionisation-quadrupole-time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS/MS) was performed to investigate the composition of DOF extracts. Online antioxidant post-column bioassay tests were applied to rapidly screen major antioxidants in DOF extracts.Results and discussion: The aqueous extract of D. officinale flowers was found to have potential antioxidant capacity, anti-cyclooxygenase2 (COX-2) effect, anti-glycation potency, and anti-aging effects. A total of 34 compounds were identified using UPLC-ESI-QTOF-MS/MS. Online ABTS radical analysis demonstrated that 1-O-caffeoyl-β-D-glucoside, vicenin-2, luteolin-6-C-β-D-xyloside-8-C-β--D-glucoside, quercetin-3-O-sophoroside, rutin, isoquercitrin, and quercetin 3-O-(6″-O-malonyl)-β-D-glucoside are the major potential antioxidants. In addition, all selected 16 compounds exerted significant ABTS radical scavenging ability and effective AGE suppressive activities. However, only certain compounds, such as rutin and isoquercitrin, displayed selective and significant antioxidant abilities, as shown by DPPH and FRAP, as well as potent COX-2 inhibitory capacity, whereas the remaining compounds displayed relatively weak or no effects. This indicates that specific components contributed to different functionalities. Our findings justified that DOF and its active compound targeted related enzymes and highlighted their potential application in anti-aging.
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