INSECT MOLECULAR BIOLOGY
·
2020
·Vol. 29
·期 5
·444-451
·
IF 2.533
L. Guo,Z.-D. Zhou,F. Mao,X.-Y. Fan,G.-Y. Liu,J. Huang,X.-M. Qiao
Drosophila suzukii (spotted wing drosophila) has become a major invasive insect pest of soft fruits in the America and Europe, causing severe yield losses every year. The female D. suzukii shows the oviposition preference for ripening or ripe fruit by cutting the hard skin with its serrated ovipositor. A recent study reported that mechanosensation is involved in the texture discrimination during egg-laying behaviour in D. suzukii . However, the underlying mechanism and molecular entity that control this behaviour are not known. The transient receptor potential (TRP) channels and degenerin/epithelial sodium channels (DEG/ENaC) are two candidate gene families of mechanically activated ion channels. Thus, we first identified TRP and DEG/ENaC genes in D. suzukii by bioinformatic analysis. Using transcriptome sequencing, we found that many TRP genes were expressed in the ovipositor in both D. suzukii and D. melanogaster , while some DEG/ENaCs showed species-specific expression patterns. Exposure to drugs targeting TRP and DEG/ENaC channels abolished the oviposition preference for harder texture in female D. suzukii . Therefore, mechanosensitive ion channels may play significant roles in the texture assessment of egg-laying behaviour in D. suzukii , which has promising implications to further research on the development of novel control measures.
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高影响因子
BMC Nephrology
·
2018
·Vol. 19
·期 1
·1-12
·
IF 2.395
Yang Huan,Male Musa,Li Yang,Wang Ning,Zhao Chenming,Jin Shan,Hu Juncheng,Chen Zhiqiang,Ye Zhangqun,Xu Hua
Background Substrate reduction therapy with analogs reduces the accumulation of substrates by inhibiting the metabolic pathways involved in their biosynthesis, providing new treatment options for patients with primary hyperoxalurias (PHs) that often progress to end-stage renal disease (ESRD). This research aims to evaluate the inhibition efficacy of Hydroxy-L-proline (HYP) analogs against calcium oxalate (CaOx) crystal formation in the Drosophila Melanogaster ( D. Melanogaster ) by comparing them with Pyridoxine (Vitamin B6). Methods Three stocks of Drosophila Melanogaster (W 118 , CG3926 RNAi, and Act5C-GAL4/CyO) were utilized. Two stocks (CG3926 RNAi and Act5C-GAL4 /CyO) were crossed to generate the Act5C > dAGXT RNAi recombinant line (F 1 generation) of D. Melanogaster which was used to compare the efficacy of Hydroxy-L-proline (HYP) analogs inhibiting CaOx crystal formation with Vitamin B 6 as the traditional therapy for primary hyperoxaluria. Results Nephrolithiasis model was successfully constructed by downregulating the function of the dAGXT gene in D. Melanogaster ( P -Value = 0.0045) . Furthermore, the efficacy of Hydroxy-L-proline (HYP) analogs against CaOx crystal formation was demonstrated in vivo using D. Melanogaster model; the results showed that these L-Proline analogs were better in inhibiting stone formation at very low concentrations than Vitamin B 6 (IC 50 = 0.6 and 1.8% for standard and dietary salt growth medium respectively) compared to N-acetyl-L-Hydroxyproline (IC 50 = 0.1% for both standard and dietary salt growth medium) and Baclofen (IC 50 = 0.06 and 0.1% for standard and dietary salt growth medium respectively). Analysis of variance (ANOVA) also showed that Hydroxy-L-proline (HYP) analogs were better alternatives for CaOx inhibition at very low concentration especially when both genetics and environmental factors are intertwined ( p < 0.0008) for the dietary salt growth medium and ( P < 0.063) for standard growth medium. Conclusion Addition of Hydroxy-L-Proline analogs to growth medium resulted in the reduction of CaOx crystals formation. These analogs show promise as potential inhibitors for oxalate reduction in Primary Hyperoxaluria.
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PHYTOMEDICINE
·
2024
·
IF 6.7
Ying Liu,Xinran Wang,Chenrong Jin,Juhui Qiao,Chenxi Wang,Leilei Jiang,Shiting Yu,Daian Pan,Daqing Zhao,Siming Wang,Meichen Liu
Background Disruption of stem cell and microbial homeostasis accelerates the aging process. Hence, maintaining these balances effectively delays aging and alleviates the symptoms of age-related diseases. Recent research indicates that targeting endoplasmic reticulum (ER) stress and immune deficiency (IMD) signalling may play a positive role in maintaining homeostasis in aging intestinal stem cells (ISC) and microbial equilibrium. Previous research has suggested that total ginsenosides (TG) derived from Panax ginseng C. A. Meyer may exhibit potential anti-aging properties by mitigating ER stress and mediating the IMD pathway. Nevertheless, it remains unclear whether TG improve ISC and microbial homeostasis by modulating ER stress and the IMD pathway to promote healthy aging. Purpose To elucidate whether TG promotes healthspan in Drosophila and its underlying molecular mechanisms, focusing on its role in regulating ER stress and the IMD pathway to maintain ISC and intestinal microbiota homeostasis. Methods High performance liquid chromatography was performed to detect the main saponin monomer in TG. Survival rate, gut length, barrier function, and feeding/excretion behaviour assays were used to evaluate the effects of TG on the lifespan and gut health of Drosophila . At the stem cell level, “esg-luciferase” reporter system, esg-GFP/delta stem cell fluorescent labelling, and phospho-histone H3+ mitotic activity assays were employed to determine whether TG prevented natural aging or oxidative stress-associated ISC over-proliferation in Drosophila . Immunofluorescence staining was used to detect the effects of TG on ER stress during aging. Overexpression or interference of ER stress target genes and their related c-Jun N-terminal kinase (JNK) gene was manipulated using gene editing technology to verify the molecular mechanism by which TG maintains age-related ISC proliferation homeostasis. Molecular docking and isothermal titration calorimetry were used to verify the direct interactions between TG and ER stress target genes. In addition, at the intestinal flora level, 16S rDNA sequencing was used to analyse the effect of TG on the diversity and abundance of Drosophila intestinal flora and the possible functional pathways involved. RT-qPCR was performed to determine whether TG mediated the expression of target genes in the IMD pathway. A dominant bacterial species-specific mono-association analysis were performed to verify whether the effects of TG on IMD target genes and ISC proliferation depended on the direct control of the dominant bacterial species. Results Our results suggest that administration of TG delays the decline in gut morphology and function in aging Drosophila . TG prevents age-associated ISC hyperproliferation by inhibiting ER stress IRE1-mediated JNK signaling. Furthermore, oral TG prevented aging-associated ISC and gut microbiota dysbiosis by remodelling the gut microbiota and inhibiting Acetobacter -mediated activation of IMD target genes. Conclusion TG promotes healthy aging by inhibiting the excessive proliferation of ISC and alleviating intestinal microbial imbalance, thereby providing new insights for the research and development of anti-aging TG products.
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CELL PROLIFERATION
·
2021
·Vol. 55
·期 1
·e13173
·
IF 6.831
Jinping Huang,Xiao Sheng,Zhangpeng Zhuo,Danqing Xiao,Kun Wu,Gang Wan,Haiyang Chen
Objectives Adult stem cells uphold a delicate balance between quiescent and active states, which is crucial for tissue homeostasis. Whereas many signalling pathways that regulate epithelial stem cells have been reported, many regulators remain unidentified. Materials and Methods Flies were used to generate tissue-specific gene knockdown and gene knockout. qRT-PCR was used to assess the relative mRNA levels. Immunofluorescence was used to determine protein localization and expression patterns. Clonal analyses were used to observe the phenotype. RNA-seq was used to screen downstream mechanisms. Results Here, we report a member of the chloride channel family, ClC - c , which is specifically expressed in Drosophila intestinal stem/progenitor cells and regulates intestinal stem cell (ISC) proliferation under physiological conditions and upon tissue damage. Mechanistically, we found that the ISC loss induced by the depletion of ClC - c in intestinal stem/progenitor cells is due to inhibition of the EGFR signalling pathway. Conclusion Our findings reveal an ISC-specific function of ClC - c in regulating stem cell maintenance and proliferation, thereby providing new insights into the functional links among the chloride channel family, ISC proliferation and tissue homeostasis.
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CURRENT BIOLOGY
·
2022
·
IF 10.9
Jingda Qiao,Shengxi Yang,Hongyan Geng,Wing-Ho Yung,Ya Ke
Summary Aversive olfactory conditioning in Drosophila is a valuable model for elucidating the mechanism of associative learning . Much effort has centered around the role of neuroplasticity at the mushroom body (MB)-mushroom body output neuron (MBON) synapses in mapping odors to specific behaviors . By electrophysiological recordings from MB neurons, we discovered a form of input-timing-dependent plasticity at the incoming synapses from projection neurons that controls the efficacy of aversive olfactory memory formation. Importantly, this plasticity is facilitated by the neural activity of PPL1, the neuronal cluster that also modulates MB-MBON connections at the output stage of MB. Unlike the MB-MBON synapses that probably utilize dopamine D1-like receptors, this neuroplasticity is dependent on D2-like receptors that are expressed mainly by γ Kenyon cells noticeably in their somato-dendritic region. The D2-like receptors recruit voltage-gated calcium channels , leading to calcium influx in the soma and dendrites of γ neurons. Together, our results reveal a previously unrecognized synaptic component of the MB circuit architecture that not only could increase the salience of a conditioning odor but also couples the process of memory encoding and valency mapping to drive-associative learning.
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Antioxidants
·
2022
·Vol. 11
·期 2
·426
·
IF 7.675
Tian Wei,Lei Wu,Xiaowen Ji,Yan Gao,Guiran Xiao
Ursolic acid (UA) is a bioactive molecule widely distributed in various fruits and vegetables, which was reported to play a therapeutic role in ulcerative colitis (UC) induced by toxic chemicals. However, the underlying mechanism has not been well clarified in vivo. Here, using aDrosophilaUC model induced by sodium dodecyl sulfate (SDS), we investigated the defensive effect of UA on intestinal damage. The results showed that UA could significantly protectDrosophilafrom the damage caused by SDS exposure. Further, UA alleviated the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) induced by SDS and upregulated the activities of total superoxide dismutase (T-SOD) and catalase (CAT). Moreover, the proliferation and differentiation of intestine stem cells (ISCs) as well as the excessive activation of the c-Jun N-terminal kinase (JNK)-dependent JAK/STAT signaling pathway induced by SDS were restored by UA. In conclusion, UA prevents intestine injury from toxic compounds by reducing the JNK/JAK/STAT signaling pathway. UA may provide a theoretical basis for functional food or natural medicine development.Keywords:ursolic acid;ulcerative colitis;JNK;JAK/STAT;Drosophila
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Frontiers in Microbiology
·
2022
·
IF 6.064
Dong Yuling,Ding Zhongfeng,Song Linxia,Zhang Desheng,Xie Changjian,Zhang Shujing,Feng Ling,Liu Hongliang,Pang Qiuxiang
Sodium benzoate (SB), the sodium salt of benzoic acid, is widely used as a preservative in foods and drinks. The toxicity of SB to the human body attracted people’s attention due to the excessive use of preservatives and the increased consumption of processed and fast foods in modern society. The SB can inhibit the growth of bacteria, fungi, and yeast. However, less is known of the effect of SB on host commensal microbial community compositions and their functions. In this study, we investigated the effect of SB on the growth and development of Drosophila melanogaster larvae and whether SB affects the commensal microbial compositions and functions. We also attempted to clarify the interaction between SB, commensal microbiota and host development by detecting the response of commensal microbiota after the intervention. The results show that SB significantly retarded the development of D. melanogaster larvae, shortened the life span, and changed the commensal microbial community. In addition, SB changed the transcription level of endocrine coding genes such as ERR and DmJHAMT. These results indicate that the slow down in D. melanogaster larvae developmental timing and shortened life span of adult flies caused by SB intake may result from the changes in endocrine hormone levels and commensal microbiota. This study provided experimental data that indicate SB could affect host growth and development of D. melanogaster through altering endocrine hormone levels and commensal microbial composition.
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CHEMICO-BIOLOGICAL INTERACTIONS
·
2026
·
IF 5.2
Zongzhong Liu,Yurong Song,Binbin Xie,Pengpeng Xia,Junlin Yang,Jiazuo Zhu,Hua Wang,Tian Wei
The ubiquitous food processing contaminant 3-monochloro-1,2-propanediol (3-MCPD) poses a potential threat to female reproductive health, yet the underlying mechanisms remain largely undefined. Here, we demonstrate that 3-MCPD exposure impairs ovarian function by disrupting germline stem cell (GSC) maintenance in Drosophila . 3-MCPD exposure dose-dependently induced ovarian atrophy, reduced fecundity, and GSC loss. Mechanistically, 3-MCPD triggered Golgi stress, leading to Mitf-dependent transcriptional upregulation of Atg9 . This, in turn, activated autophagy, which selectively degraded E-cadherin, a critical adhesion molecule for GSC maintenance. Notably, GSC-specific E-cadherin overexpression or Atg9 knockdown effectively rescued 3-MCPD-induced ovarian defects. We further identified a direct physical interaction between Atg9 and E-cadherin, corroborated by immunofluorescence co-localization and AlphaFold3 modeling, which revealed high-affinity binding interfaces that likely mediate this selective autophagic degradation. Both E-cadherin and Atg9 exhibit high evolutionary conservation, underscoring the translational relevance of our findings. This study delineates a novel Golgi stress-Mitf-Atg9 axis through which a prevalent food contaminant compromises female fertility, thereby identifying potential targets for intervention.
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EMBO JOURNAL
·
2025
·
IF 8.3
KongDu,LiXiaoqin,ZhaoSihua,WangChenliang,CaiZixin,SongSha,GuoYifan,KuangXiaoyu,WangXianping,LiuWenhan,LiuPeng,GuoXiaowei,XuWenyan,WangYirong,ZhaoBin,JinBin,HeLi,MaXianjue
Interorgan communication is vital for tissue homeostasis and health in multicellular organisms, and its disruption can lead to diseases such as cancer. Adipose tissue acts as a key endocrine center, secreting cytokines that influence remote organs. Despite clear links between obesity and increased cancer risk, the underlying mechanisms are unclear. Here, utilizing a Drosophila genetic model combining Gal4-UAS and QF-QUAS tissue-specific transgene expression systems, we reveal that adipose-secreted Spz5 ligand promotes distal epithelial tumor overgrowth and invasion. Mechanistically, Spz5 binds to tumor cell Toll-6 receptors, triggering the degradation of the endocytic adaptor protein AP-2α via Mib1-mediated ubiquitination. Consequently, impaired endocytosis leads to Smoothened (Smo) accumulation on the cell membrane and subsequent activation of the Hedgehog (Hh) pathway. This abnormal Hh activation synergizes with the oncogenic Yorkie (Yki) to drive tumor growth and invasion. Furthermore, tumor-derived Unpaired ligands (Upds) activate the JAK-STAT pathway in the fat bodies, which leads to Hippo pathway-dependent upregulation of spz5 expression. Thus, our study provides insights into the complex regulatory mechanisms by which systemic interorgan communication influences tumor progression.
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MOLECULAR ECOLOGY
·
2023
·
IF 4.9
Xiao-Li Bing,Zi-Jian Liang,Jia Tian,Xue Gong,Shao-Qiu Huang,Jie Chen,Xiao-Yue Hong
Insects are rich in various microorganisms, which play diverse roles in affecting host biology. Although most Drosophila species prefer rotten fruits, the agricultural pest Drosophila suzukii attacks ripening fruits before they are harvested. We have reported that the microbiota has positive and negative impacts on the agricultural pest D. suzukii on nutrient-poor and -rich diets, respectively. On nutrient-poor diets, microbes provide protein to facilitate larval development. But how they impede D. suzukii development on nutrient-rich diets is unknown. Here we report that Acetobacter pomorum (Apo), a commensal bacterium in many Drosophila species and rotting fruit, has several detrimental effects in D. suzukii . Feeding D. suzukii larvae nutrient-rich diets containing live Apo significantly delayed larval development and reduced the body weight of emerged adults. Apo induced larval immune responses and downregulated genes of digestion and juvenile hormone metabolism. Knockdown of these genes in germ-free larvae reproduced Apo-like weakened phenotypes. Apo was confirmed to secrete substantial amounts of gluconic acid. Adding gluconic acid to the D. suzukii larval diet hindered larval growth and decreased adult body weight. Moreover, the dose of gluconic acid that adversely affected D. suzukii did not negatively affect Drosophila melanogaster , suggesting that D. suzukii is less tolerant to acid than D. melanogaster . Taken together, these findings indicate that D. suzukii is negatively affected by gluconic acid, which may explain why it prefers ripening fruit over Apo-rich rotting fruit. These results show an insect's tolerance to microbes can influence its ecological niche.
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PHYTOMEDICINE
·
2026
·
IF 11.3
Quanyong Wu,Boya Ouyang,Wanning Ma,Yuxuan Zhao,Jingyimei Liang,Yi Yuan,Hui Cao,Jesus Simal-Gandara,Shaoping Nie,Jianbo Xiao
Background Resveratrol is a polyphenolic compound with reported anti-aging properties that exhibit complex and context-dependent biological effects, while its potential multigenerational influence remains unclear. Objectives To investigate the stage-specific effects of resveratrol on development and lifespan in Drosophila melanogaster and to determine whether resveratrol-associated longevity benefits can extend across generations through metabolic and epigenetic regulation. Methods Drosophila melanogaster were exposed to 50 μg/mL resveratrol (ER50) either during embryonic development or during adulthood. Developmental timing, lifespan, hormonal and nutrient-sensing signaling pathways, stress-response and autophagy pathways, mTOR signaling, metabolomic profiles, histone modifications, histone-modifying enzyme expression, and multigenerational lifespan effects were evaluated. Results Embryonic exposure to ER50 accelerated pupation and adult emergence but shortened adult lifespan. These effects were accompanied by activation of the juvenile hormone–insulin/IGF signaling axis and impaired antioxidant capacity. In contrast, adult ER50 supplementation extended lifespan in female flies and was associated with enhanced stress-response and autophagy pathways together with suppression of mTOR signaling. Mechanistically, longevity-promoting effects in adults coincided with increased glycolysis and pentose phosphate pathway activity, citrate accumulation, and remodeling of one-carbon metabolism, indicating broad reprogramming of energy and redox metabolism. These metabolic changes were accompanied by increased H3K9 acetylation (H3K9ac), upregulation of the histone acetyltransferase-related factor nej , and downregulation of Ash1 , suggesting that resveratrol promotes lifespan extension through coordinated regulation of metabolic state and chromatin remodeling. Notably, maternal adult exposure to ER50 was associated with lifespan extension in female offspring across two subsequent generations, together with persistent metabolic alterations and chromatin changes. Conclusions Resveratrol exhibits stage- and sex-dependent effects in Drosophila and functions as a metabolic–epigenetic modulator. Adult supplementation is associated with extended female lifespan and multigenerational longevity effects, potentially mediated through coordinated regulation of energy metabolism and chromatin homeostasis. Download: Download high-res image (181KB) Download: Download full-size image
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ISME Journal
·
2021
·Vol. 15
·期 11
·3239-3257
·
IF 10.302
Zhang Jiao,Wang Fei,Yuan Bo,Yang Lei,Yang Yi,Fang Qi,Kuhn Jens H.,Song Qisheng,Ye Gongyin
We identified a 9332-nucleotide-long novel picornaviral genome sequence in the transcriptome of an agriculturally important parasitoid wasp ( Pachycrepoideus vindemmiae (Rondani, 1875)). The genome of the novel virus, Rondani’s wasp virus 1 (RoWV-1), contains two long open reading frames encoding a nonstructural and a structural protein, respectively, and is 3’-polyadenylated. Phylogenetic analyses firmly place RoWV-1 into the dicistrovirid genus Cripavirus . We detected RoWV-1 in various tissues and life stages of the parasitoid wasp, with the highest virus load measured in the larval digestive tract. We demonstrate that RoWV-1 is transmitted horizontally from infected to uninfected wasps but not vertically to wasp offspring. Comparison of several important biological parameters between the infected and uninfected wasps indicates that RoWV-1 does not have obvious detrimental effects on wasps. We further demonstrate that RoWV-1 also infects Drosophila melanogaster (Meigen, 1830), the hosts of the pupal ectoparasitoid wasps, and thereby increases its pupal developmental duration and fecundity, but decreases the eclosion rate. Together, these results suggest that RoWV-1 may have a potential benefit to the wasp by increasing not only the number of potential wasp hosts but also the developmental time of the hosts to ensure proper development of wasp offspring.
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高影响因子
危险品化学品经营许可证（带存储）