SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Xiluan Yan,Shiyun Cheng,Yipi Xiao,Shuangbin Wu,Hongyi Mu,Zhiying Shi,Liang Guo,Fanrong Ai,Xiangjuan Zheng
Metal-organic gels (MOGs) are a type of metal-organic colloid material with a large specific surface area, loose porous structure, and open metal active sites. In this work, FeNi-MOGs were synthesized by the simple one-step static method, using Fe(III) and Ni(II) as the central metal ions and terephthalic acid as the organic ligand. The prepared FeNi-MOGs could effectively catalyze the chemiluminescence of luminol without the involvement of H 2 O 2 , which exhibited good catalytic activity. Then, the multifunctional detected platform was constructed for the detection of GSH and Hg 2+ , based on the antioxidant capacity of GSH, and the strong affinity between mercury ion (Hg 2+ ) and GSH which inactivated the antioxidant capacity of GSH. The experimental limits of detection (LOD) for GSH and Hg 2+ were 76 nM and 210 nM, and the detection ranges were 2–100 μM and 8–4000 μM, respectively. The as-proposed sensor had good performance in both detection limit and detection range of GSH and Hg 2+ , which fully met the needs of daily life. Surprisingly, the sensor had low detection limits and an extremely wide detection range for Hg 2+ , spanning five orders of magnitude. Furthermore, the detection of mercury ions in actual lake water and GSH in human serum showed good results, with recovery rates ranging from 90.10 % to 105.37 %, which proved that the method was accurate and reliable. The as-proposed sensor had great potential as the platform for GSH and Hg 2+ detection applications.
研究领域:
分析化学
·
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Ning Wang,Chenyu Liu,Weihe Yao,Hengjun Zhou,Simiao Yu,Hailiang Chen,Weihong Qiao
Constructing highly efficient and multifunctional nanoparticles to overcome the multiple challenges of targeted drug delivery is a new strategy urgently needed in tumor therapy. Here, we synthesized pH-responsive prodrug ( PEG 2K -NH -N- DOX ), GSH-responsive prodrug ( PEG 2K -S-S-CPT ), folate-receptor targeting polymers ( FA-PEG 2K -L8 , FA-PEG 2K -TOS) and T1-enhanced magnetic resonance imaging contrast agents ( Gd-DTPA-N16−16) , used to encapsulate combrestatinA4 (CA4) to prepare multifunctional nanoparticles ( FTDCAG NPs). Unlike other nanoparticles, FTDCAG NPs contains three drugs with the ability to control the release in time and space, which can maximize the effectiveness of precise cancer chemotherapy. We first confirmed that specific binding between FTDCAG NPs and overexpressed folate-receptor cells by flow cytometry and confocal laser scanning microscopy. We then investigated the spatiotemporally controlled release ability of FTDCAG NPs loaded with doxorubicin (DOX), CA4 and camptothecin (CPT). Relative to pH = 7.4, the release efficiency of CA4 in the pH = 6.5 increased by 63.4 %. The first released CA4 is able to destroy the angiogenesis and help tumor cells to be exposed to the remaining FTDCG NPs. After being internalized into the tumor cells, FTDCG NPs is disassembled and the CPT and DOX were released due to the increase of intracellular GSH concentration and the decrease of pH value. Besides, the relaxation time of FTDCAG NPs is 3.86 times that of clinical Gd-DTPA, and the in vitro and vivo T1-weighted imaging is brighter, which can be used to trace the nanoparticles by MRI. Therefore, FTDCAG NPs provide an efficient strategy for the design of multifunctional drug delivery systems for enhancing antitumor efficacy.
研究领域:
纳米医学
·
癌症治疗
·
药物递送系统
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Siao Yang,Xiaoxiao Hu,Zhengze Yong,Qingqing Dou,Cuilu Quan,Hong-Bo Cheng,Mo Zhang,Jing Wang
Photoimmunotherapy represents an innovative approach to enhancing the efficiency of immunotherapy in cancer treatment. This approach involves the fusion of immunotherapy and phototherapy (encompassing techniques like photodynamic therapy (PDT) and photothermal therapy (PTT)). Boron-dipyrromethene (BODIPY) has the potential to trigger immunotherapy owing to its excellent PD and PT efficiency. However, the improvements in water solubility, bioavailability, PD/PT combined efficiency, and tumor tissue targeting of BODIPY require introduction of suitable carriers for potential practical application. Herein, a disulfide bond-based hollow mesoporous organosilica (HMON) with excellent biocompatibility and GSH-responsive degradation properties was used as a carrier to load a bithiophene Aza-BODIPY dye (B5), constructing a sample chemotherapy reagent-free B5@HMON nanoplatform achieving triple-synergistic photoimmunotherapy. HMON, involving disulfide bond , is utilized to improve water solubility, tumor tissue targeting, and PD efficiency by depleting GSH and enhancing host-guest interaction between B5 and HMO. The study reveals that HMON’s large specific surface area and porous properties significantly enhance the light collection and oxygen adsorption capacity. The HMON’s rich mesoporous structure and internal cavity achieved a loading rate of B5 at 11 %. It was found that the triple-synergistic nanoplatform triggered a stronger anti-tumor immune response, including tumor invasion, cytokine production, calreticulin translocation, and dendritic cell maturation, eliciting specific tumor-specific immunological responses in vivo and in vitro . The BALB/c mouse model with 4T1 tumors was used to assess tumor suppression efficiency in vivo , showing that almost all tumors in the B5@HMON group disappeared after 14 days. Such a simple chemotherapy reagent-free B5@HMON nanoplatform achieved triple-synergistic photoimmunotherapy.
研究领域:
癌症治疗
·
纳米技术
·
生物医学工程
·
免疫学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Anti-Cancer Agents in Medicinal Chemistry
·
2013
·Vol. 13
·期 8
·1280-1290
·
IF 2.61
Wen Hua Yang,Zhen Zhang,Yang Hu Sima,Jian Zhang,Jian Wen Wang
Glaucocalyxin (Gla) A-C are major ent-kauranoid diterpenoids isolated from Rabdosia japonica var. glaucocalyx, a plant used in Chinese traditional medicine as an antitumor and anti-inflammatory agent. The present investigation was carried out to observe whether cellular reduced glutathione (GSH) plays important roles in Gla -induced cytotoxicity. Among major ent-kauranoid diterpenoids isolated, Gla A and B dose-dependently decreased the growth of HL-60 cells with an IC50 of approximately 6.15 and 5.86 µM at 24 h, respectively. Both Gla A and B could induce apoptosis, G2/M-phase cycle arrest, DNA damage and the accumulation of reactive oxygen species (ROS) in HL-60 cells. Moreover, Gla A, B caused rapid decrease of the intracellular GSH content, while inhibition of cellular GSH synthesis by buthionine sulfoximine (BSO) augmented the induced cytotoxicity and apoptosis in HL-60 cells. On the other hand, the administration of GSH or GSH precursor N-acetyl-cysteine (NAC) could rescue Gla A, B-depleted cellular GSH, and abrogate the induced cytotoxicity, G2/M-phase cycle arrest, DNA damage and ROS accumulation in HL-60 cells. Furthermore, Gla A, B decreased the activity of the GSH-related enzymes including glutathione reductase (GR) and glutathione peroxidase (GPX). These data suggest that the intracellular GSH redox system plays important roles in regulating the Gla A, B-induced cytotoxicity on HL-60 cells.
研究领域:
药理学
·
生物化学
·
肿瘤学
·
细胞生物学
·
天然产物化学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 46
·19462-19471
·
IF 2.7
Wei Guo,Yunhan Huang,Jingrui Chang,Xinyu Wang,Bo Lu
Mesoporous organosilica nanoparticles (MONs) are promising drug carriers with excellent biocompatibility and biodegradability. In the context of the bacterial infection microenvironment, a hyaluronidase (HAase)/glutathione (GSH) dual-responsive degradable nanoplatform based on MON (ICG/CIP@MON@PEI-HA) has been developed for the multimodal treatment of bacterial infection. In this work, ciprofloxacin (CIP) and indocyanine green (ICG) are physically adsorbed into the MON, polyethyleneimine (PEI) is electrostatically adsorbed onto the MON surface, and hyaluronic acid (HA) is grafted onto the amino groups of PEI via the amide bonds. The overexpression of HAase at bacterial infection sites enables HA shell degradation, and positively charged PEI is exposed, which facilitates nanoparticle binding to negatively charged bacteria. Also, owing to the protonation of amine groups, PEI is swelled in the acidic environment of bacterial infection, which favours drug release. Subsequently, overexpressed GSH breaks the disulfide bonds in the MON, triggering structural degradation that inhibits carrier accumulation and accelerates drug release even further. According to in vitro antibacterial evaluations, the antibacterial effect can be enhanced to 100% when phototherapy and pharmaceutical therapy are combined. In vitro cytotoxicity assays have demonstrated that ICG/CIP@MON@PEI-HA possesses excellent biocompatibility. Therefore, this study offers a potential approach for developing biodegradable nanoplatforms for the combined treatment of bacterial infection.
研究领域:
纳米技术
·
生物医学工程
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2023
·
IF 9.5
Lihua Li,Zefeng Lin,Xingyi Xu,Wanshun Wang,Hu Chen,Zhibin Feng,Zhongmin Yang,Jianhua Hao
Nanozymes have brought enormous opportunities for disease theranostics. Here, a self-enhanced catalytic nanocrystal based on a bismuth–manganese core–shell nanoflower containing glucose oxide (GOx), termed BDS-GOx@MnOx, was designed for 4T1 tumor theranostics in vitro and in vivo. The BDS-GOx@MnOx nanozymes enable enhanced starvation treatment (ST) and chemotherapy (CDT) with high efficacy and exhibit sensitive tumor microenvironment (TME) responsive character for tumor therapy as well as for tumor-enhanced computer tomography (CT) and magnetic resonance (MR) diagnostic imaging. The characters and mechanism of the BDS-GOx@MnOx nanozymes have also been systematically studied and revealed.
研究领域:
纳米技术
·
癌症治疗
·
生物医学工程
·
诊断成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Hui Zhang,Xuelin You,Ruihan Liu,You Wang,Fang Han,Juanyuan Hao,Yu Li
Cuproptosis efficiency is severely limited by insufficient Cu+ contents in tumors due to the easy oxidation of Cu+ into Cu2+ and the copper efflux mechanism. Herein, we develop a copper nanocarrier (MoSe2/CuO2) that can significantly enhance intracellular Cu+ for self-boosting cuproptosis-based synergistic antitumor therapy. Upon endocytosis by cancer cells, the acidic tumor microenvironment (TME) triggers the degradation of MoSe2/CuO2 to release Cu2+. MoSe2 then acts as a cocatalyst that promotes the reduction of released Cu2+ to Cu+, while oxidizing active Mo4+ to Mo6+, thereby triggering cuproptosis. Importantly, the produced Mo6+ is further returned to Mo4+ by endogenous glutathione (GSH) depletion, enhancing the cocatalytic effects of MoSe2 and resulting in a continuous Cu+ generation for self-boosting cuproptosis. Meanwhile, GSH-enhanced cocatalysis and MoSe2-mediated photothermal effect can significantly promote Cu-Fenton reactions, which not only downregulate Cu-ATPase to reduce Cu+ efflux and aggravate cuproptosis with higher efficacy but also activate caspase-3 to promote cell apoptosis. MoSe2/CuO2 exhibits excellent antitumor effects (94.9% tumor growth inhibition) and biosafety effects in vitro and in vivo. This work presents a strategy that enables high levels of Cu+ delivery by enhancing generation and reducing efflux for self-boosting cuproptosis–apoptosis synergistic therapy.
研究领域:
纳米技术
·
癌症研究
·
生物医学工程
·
治疗学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Xiaoyong song,Zejun Lin,Guozhou Cao,Xu Zhang,Nan Wang,Xiao–kun Ouyang
Nanomedicines can overcome several limitations of traditional chemotherapeutics such as imprecise targeting and severe side effects. However, challenges remain in selective delivery, targeted accumulation, and spatiotemporal control of release. This study aims to develop a pH- and glutathione (GSH)-responsive nanodrug delivery system that integrates photothermal therapy (PTT) and chemotherapy. We designed and synthesized degradable hollow mesoporous organosilica nanoparticles (DHMONs) with tetrasulfide bonds, which were self-assembled with copper sulfide nanoparticles (CuS NPs) via surface modification. These DHMONs@CuS nanoparticles utilize the hollow structure for a large surface area and high drug-loading capacity, enabling the encapsulation of the chemotherapeutic drug doxorubicin (DOX) for synergistic photothermal-chemotherapeutic treatment. Additionally, the surface modification of these nanoparticles with hyaluronic acid (HA) facilitates active targeting of tumor cells overexpressing CD44 receptors. The system exhibits dual responsiveness to pH and GSH, aiding precise drug release within the tumor microenvironment. Following studies demonstrate that this nanosystem effectively inhibits tumor growth, showing promising prospects for application in multimodal cancer treatment.
研究领域:
纳米医学
·
癌症治疗
·
药物输送系统
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Materials Today Bio
·
2022
·
IF 10.761
Yi Feng,Hanxi Zhang,Xiaoxue Xie,Yu Chen,Geng Yang,Xiaodan Wei,Ningxi Li,Mengyue Li,Tingting Li,Xiang Qin,Shun Li,Fengming You,Chunhui Wu,Hong Yang,Yiyao Liu
Therapeutic approaches of combining conventional photodynamic therapy (PDT) with other adjuvant treatments to sensitize PDT represent an appealing strategy. Herein, a novel synergetic “nanobomb” strategy based on glutathione (GSH)-responsive biodegradation was proposed to effectively destroy tumors expeditiously and accurately. This “nanobomb” was rationally constructed via the simultaneous encapsulation of methylene blue (MB) and l -arginine (L-Arg) into polyethylene glycol (PEG) modified mesoporous organosilicon nanoparticles (MON). The resulting L-Arg/ [email protected] initially exhibited prolonged blood circulation, improved bioavailability, and enhanced tumor accumulation in mice after tail vein injection according to the pharmacokinetic investigations, before the nanoparticles were entirely excreted. Under laser irradiation, L-Arg/ [email protected] produced remarkable reactive oxygen species (ROS) directly for PDT therapy, while a portion of ROS may oxidize L-Arg to generate nitric oxide (NO) not only for gas therapy (GT) but also serve as a biological messenger to regulate vasodilation to alleviate the tumor hypoxia. Subsequently, the rapidly released NO was further oxidized to reactive nitrogen species, which together with ROS promote immunogenic cell death by inducing G2/M cell-cycle arrest and apoptosis in cancer cells, and eventually resulting in enhanced anti-tumor immune responses. Moreover, the GSH depletion in tumor tissues induced by L-Arg/ [email protected] biodegradation can cooperate with GT to amplify the therapeutic effect of PDT. These results demonstrate that this “nanobomb” provides new ideas for clinical translation to treat tumor patients in terms of synergistic PDT-GT nanotherapy in hypoxic-solid tumors.
研究领域:
癌症治疗
·
纳米技术
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2022
·
IF 10.633
Junqin Li,Wenhua Yi,Yuze Luo,Ke Yang,Lidan He,Caiyun Xu,Le Deng,Dinggeng He
Nanozyme-based chemodynamic therapy (CDT) has shown tremendous potential in the treatment of bacterial infections. However, the CDT antibacterial efficacy is severely limited by the catalytic activity of nanozymes or the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Herein, a versatile hybrid nanozyme (MoS 2 /CuO 2 ) is rationally constructed by simply decorating ultrasmall CuO 2 nanodots onto lamellar MoS 2 platelets of hydrangea-like MoS 2 nanocarrier via a covalent Cu-S bond. The MoS 2 /CuO 2 nanozyme exhibits the peroxidase-mimic activity for catalytically converting H 2 O 2 produced by acid-triggered decomposition of the decorated CuO 2 into hydroxyl radical (•OH). Meanwhile, the MoS 2 /CuO 2 can consume GSH overexpressed in the infection sites via redox reaction mediated by polyvalent transition metal ions (Cu 2+ and Mo 6+ ) for enhanced CDT. More importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by a co-catalytic reaction based on the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 nanozymes possesses a desirable catalytic property, as well as a remarkably improved antibacterial efficiency both in vitro and in vivo . Taken together, this study proposes a synergetic multiple enhancement strategy to successfully construct the versatile hybrid nanozymes for intensive in vivo PTT/CDT dual-mode anti-infective therapy. Statement of significance Chemodynamic therapy (CDT) has shown great potentialities in the treatment of bacterial infections, while its therapeutic efficiency is severely limited by the infection microenvironments such as insufficient hydrogen peroxide (H 2 O 2 ) and overexpressed glutathione (GSH). Here, we rationally construct a hybrid nanozyme (MoS 2 /CuO 2 ) with peroxidase-like activity that can enhance CDT by regulating local microenvironments, that is, simultaneously self-supplying H 2 O 2 and consuming GSH. Importantly, MoS 2 support can promote the conversion of Cu 2+ to Cu + by the Mo 4+ /Mo 6+ redox couples, and provide photonic hyperthermia (PTT) to augment the peroxidase-mimic activity. The developed MoS 2 /CuO 2 shows desirable PTT/CDT dual-mode antibacterial efficacy both in vitro and in vivo . This study proposes a versatile hybrid nanozyme with multiple enhancement effects for intensive in vivo anti-infective therapy.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
治疗学
·
抗菌研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROCESS BIOCHEMISTRY
·
2024
·
IF 3.7
Weiheng Yan,Limeng Wu,Chongzhou Sun,Shuaidao Wang,Qiang Dai
ABSTRACT A nano-drug delivery system that is responsive to glutathione (GSH) and combines chemotherapy (CT) and photodynamic therapy (PDT) was designed precisely for treating melanoma. The carboxymethyl chitosan (CMC), a water-soluble and biocompatible nanocarrier, was used to load the chemotherapeutic small-molecule drug camptothecin (CPT) and the photosensitizer Rhodamine-B (RhB). The development of dual drug combo (CCR NPs) nanoparticles with a mean particle diameter of 170 ± 2.2 nm in aqueous solution has been achieved using ultrasonic self-assembly. Nanoparticle disintegration and camptothecin (CPT) release occur as CPT, sensitive to glutathione (GSH), breaks in cancer cells with excellent GSH levels. The RhB encapsulated in the CCR NPs was released quickly to attain successive CPT release. The CPT dramatically lowers tumor cell GSH levels, inhibiting the antioxidant effect of GSH on photosensitizers' ROS production capabilities and improving PDT synergistically. Nanoparticle diameter, zeta potential, and outstanding stability were all demonstrated by the CCR NPs. In vitro toxicity study on A375 human melanoma cells showed that CPT and RhB were highly selective (SI=4.25) and had a synergic effect (CI=0.24). Biochemical staining methods confirmed the morphological changes and nuclear damages. Apoptosis was confirmed by FITC-Annuxin V and PI staining by flow cytometer. Investigating drug delivery systems integrating RhB and CPT represents a valuable novel strategy for treating melanoma cancer.
研究领域:
纳米技术
·
药学
·
癌症研究
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-17
·
IF 10.6
Chen Wenting,Hu Fangfang,Gao Qian,Zheng Caiyun,Bai Que,Liu Jinxi,Sun Na,Zhang Wenhui,Zhang Yanni,Dong Kai,Lu Tingli
Chemodynamic therapy (CDT) based on intracellular Fenton reaction to produce highly cytotoxic reactive oxygen species (ROS) has played an essential role in tumor therapy. However, this therapy still needs to be improved by weakly acidic pH and over-expression of glutathione (GSH) in tumor microenvironment (TEM), which hinders its future application. Herein, we reported a multifunctional bimetallic composite nanoparticle MnO2@GA-Fe@CAI based on a metal polyphenol network (MPN) structure, which could reduce intracellular pH and endogenous GSH by remodeling tumor microenvironment to improve Fenton activity. MnO2 nanoparticles were prepared first and MnO2@GA-Fe nanoparticles with Fe3+ as central ion and gallic acid (GA) as surface ligands were prepared by the chelation reaction. Then, carbonic anhydrase inhibitor (CAI) was coupled with GA to form MnO2@GA-Fe@CAI. The properties of the bimetallic composite nanoparticles were studied, and the results showed that CAI could reduce intracellular pH. At the same time, MnO2 could deplete intracellular GSH and produce Mn2+ via redox reactions, which re-established the TME with low pH and GSH. In addition, GA reduced Fe3+ to Fe2+. Mn2+ and Fe2+ catalyzed the endogenous H2O2 to produce high-lever ROS to kill tumor cells. Compared with MnO2, MnO2@GA-Fe@CAI could reduce the tumor weight and volume for the xenograft MDA-MB-231 tumor-bearing mice and the final tumor inhibition rate of 58.09 ± 5.77%, showing the improved therapeutic effect as well as the biological safety. Therefore, this study achieved the high-efficiency CDT effect catalyzed by bimetallic through reshaping the tumor microenvironment.Graphical Abstract
研究领域:
癌症治疗
·
纳米技术
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）