Journal of Orthopaedic Surgery and Research
·
2020
·Vol. 15
·期 1
·1-8
·
IF 1.777
Zhang Wei,Cui Sheng-Yu,Yi Hong,Zhu Xin-Hui,Liu Wei,Xu You-Jia
Background The dysregulation of proliferation and apoptosis plays a significant role in the pathogenesis of postmenopausal osteoporosis (PO). MicroRNAs play an important role in regulating apoptosis of MC3T3-E1 cells. However, the role and potential mechanism of miR-708 for regulating H2O2-induced apoptosis is unknown. This study aimed to investigate the protective function of miR-708 in H2O2-induced apoptosis of MC3T3-E1 osteoblasts.Methods MC3T3-E1 was co-cultured with H2O2 for 8 h, then, flow cytometry, malondialdehyde (MDA), and glutathione peroxidase (Gpx) levels were measured to establish the oxidative model. MiRNA microarray was performed to assess differentially expressed miRNAs between control and H2O2-treated MC3T3-E1 cells. We then performed RT-PCR to identify the relative expression of miR-708 and PTEN. After transfected MC3T3-E1 with miR-708 mimics, flow cytometry, MDA, and Gpx level were performed to identify the apoptosis rate and oxidative stress in these groups. Furthermore, we small interfering RNA of PTEN to identify the role of PTEN in H2O2-induced apoptosis of MC3T3-E1 cells.Results H2O2 (100 nM) could significantly induce the apoptosis of MC3T3-E1 cells. Moreover, H2O2 could significantly increase the MDA level and downregulated Gpx level. RT-PCR found that H2O2 significantly decrease the level of miR-708. Compared with H2O2 group, H2O2 + miR-708 mimic significantly decreased the apoptosis rate.Conclusion smiR-708 plays a protective role in H2O2-induced MC3T3-E1 osteoblasts apoptosis and its protective effect is proceeded by regulating ROS level and PTEN expression level.
研究领域:
分子生物学
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细胞生物学
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骨质疏松研究
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 31
·15032-15041
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IF 3.925
Xiaona Zhu,Zegao Wang,Mingyan Gao,Yuqing Wang,Jing Hu,Zhengxun Song,Zuobin Wang,Mingdong Dong
Hydrogen peroxide (H2O2), as a biomarker in a series of physiological processes, plays a crucial role in investigating the pathogenesis of some diseases. The highly sensitive and selective detection of H2O2 release from living cells has aroused tremendous research interests. Herein, a non-enzymatic electrochemical H2O2 biosensor was constructed based on AgPt bimetallic nanoparticle-structured MoS2 nanohybrid (AgPt/MoS2) as an electrocatalyst. The AgPt/MoS2 nanohybrid was successfully synthesized by a facile wet-chemical method. Compared with AgPt nanoparticles, the AgPt/MoS2 nanohybrid exhibited significant enhancement in catalytic performance towards H2O2. The main factor is that MoS2 nanosheets as a supporting material promote AgPt nanoparticles to expose more active sites. The electrochemical response of the AgPt/MoS2 hybrid-based biosensor to H2O2 was linear in the range of 20 μM–4 mM, and the detection limit was calculated as 1.0 μM. To prove practicality, the biosensor was further applied to detect H2O2 release from living SMMC-7721 cells. These results indicate the potential applications of the developed biosensor in monitoring physiological processes.
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE & TECHNOLOGY
·
2021
·
IF 9.028
Shuhui Sun,Chao Shan,Zhichao Yang,Shu Wang,Bingcai Pan
Phosphonate is an important category of highly soluble organophosphorus in contaminated waters, and its oxidative transformation into phosphate is usually a prerequisite step to achieve the in-depth removal of the total phosphorus. Currently, selective oxidation of phosphonate into phosphate is urgently desired as conventional advanced oxidation processes suffer from severe matrix interferences. Herein, we employed 1-hydroxyethylidene-1,1-diphosphonic acid (HEDP) as a model phosphonate and demonstrated its efficient and selective oxidation by the Cu(II)/H2O2 process at alkaline pH. In the presence of trace Cu(II) (0.020 mM), 90.8% of HEDP (0.10 mM) was converted to phosphate by H2O2 in 30 min at pH 9.5, whereas negligible conversion was observed by UV/H2O2 or a Fenton reaction (pH = 3.0). The oxidation of HEDP by Cu(II)/H2O2 was insignificantly affected by natural organic matters (10.0 mg TOC/L) and various anions including chloride, sulfate, and nitrate (10.0 mM). The complexation of Cu(II) with HEDP coupling Cu(III) produced in situ enabled an intramolecular electron transfer process, which features high selective oxidation. Selective degradation of HEDP was further validated by adding stoichiometric H2O2 into an industrial effluent, where the existing Cu(II) could serve as the catalyst. This study also provides a successful case to trigger selective oxidation of trace pollutants of concern upon synergizing with the nature of the contaminated water.
研究领域:
环境科学
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水处理技术
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Dongdong Liu,Dengqian Chen,Zhengkai Hao,Yibo Tang,Lipeng Jiang,Tianqi Li,Bing Tian,Cuiping Yan,Yuan Luo,Boyin Jia
In this work, a comparative study of efficient degradation of Rhodamine B (RhB) in CoFe 2 O 4 /H 2 O 2 and CoFe 2 O 4 /PMS systems was performed. Batch experiments indicated that the RhB degradation rate of CoFe 2 O 4 /H 2 O 2 system reached 95.5% at 90 min under the condition of 0.5 g L −1 of CoFe 2 O 4 dosage, 10 mM of H 2 O 2 concentration and 3.0 of initial pH. At certain conditions of initial pH = 7.0, 0.3 g L −1 of CoFe 2 O 4 dosage, 7 mM of PMS concentration, CoFe 2 O 4 /PMS system could completely degrade RhB within 90 min. EPR and quenching experiments indicated that •OH was the main active species of CoFe 2 O 4 /H 2 O 2 system, and •OH, SO 4 • - , •O 2 − and 1 O 2 participated in RhB degradation of CoFe 2 O 4 /PMS system. The circulate of Co(II)/Co(III) and Fe(II)/Fe(III) on the CoFe 2 O 4 surface promoted the formation of free radical species in the two system. In CoFe 2 O 4 /PMS system, the formed •O 2 − and SO 5 • - realized the generation of non-free radical species ( 1 O 2 ). The LC-MS results indicated that N -de-ethylation, chromophore cleavage, opening rings and mineralization were the main steps for the RhB degradation of the two systems. After five cycles of degradation experiment, the CoFe 2 O 4 /H 2 O 2 and CoFe 2 O 4 /PMS systems still maintained the high degradation rate (85.2% and 92.4%) and low mass loss (2.7% and 3.09%). In addition, CoFe 2 O 4 /PMS system had better potential value for the actual water and multi-pollutant degradation than CoFe 2 O 4 /H 2 O 2 system. Finally, the toxicity analysis and cost assessment of the two oxidation systems were preliminarily evaluated.
研究领域:
环境科学
·
化学工程
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DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Boqiang Li,Fei Yang,Baiyang Chen,Juan Li,Lijuan Zhu,Wen-Tao Li
Dissolved organic nitrogen (DON) plays a key role in many biogeochemistry and engineering processes such as forming nitrogenous disinfection byproducts . However, detecting aqueous DON at trace levels is challenging currently because conventional DON conversion methods have very high method detection limits (MDL). In addition, DON is measured indirectly by subtracting dissolved inorganic nitrogen (DIN) from total dissolved nitrogen (TDN), which can propagate analytical errors of each analyte. In order to solve these issues, we isolated DON from DIN with electrodialysis before and herein tested vacuum ultraviolet (VUV) in tandem with several oxidants to convert DON completely into nitrate for subsequent N analysis. Results showed that H 2 O 2 was more suitable than chlorine and persulfate because VUV/chlorine or VUV/persulfate is either inefficient to convert DON or subjected to nonnegligible N loss. To verify the efficiency, we evaluated the effects of typical water and operating variables on the conversions of four model DON compounds and their yields of nitrate. Under optimal conditions (pH 10.3 and 500 mg/L H 2 O 2 ), the process converted DON completely into nitrate within just 60 min. Compared to conventional TDN analytical methods, the VUV/H 2 O 2 method features not only better analytical precision but also lower MDL because the formed nitrate can be analyzed at very low MDL by ion chromatography (IC). So, this approach moves one step further to achieve a conceptually new DON analytical method by coupling electrodialysis, VUV/H 2 O 2 , and IC.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
Water
·
2024
·Vol. 16
·期 22
·3235
·
IF 3
Qiao Xu,Fenfen Yan,Yujie Fan,Miao Gong
This work evaluated the alterations in the levels and types of polycyclic aromatic hydrocarbons (PAHs) within both liquid and solid products throughout the process of the catalytic supercritical water gasification of dewatered sewage sludge to examine the catalytic effect of various catalysts and the inhibit reaction pathways. The addition of Ni, NaOH, Na2CO3, H2O2, and KMnO4reduced the concentrations of PAHs, with Ni and H2O2showing the best performance. The concentrations of PAHs, especially higher-molecular-weight compounds in the residues, decreased sharply as the H2O2amount increased. At a 10 wt% H2O2addition, the levels of PAHs in the liquid and solid products were reduced by 91% and 88%, respectively. High-ring PAHs were not detected in the residues as the H2O2amount increased to an 8 wt%. H2O2addition evidently inhibits PAH formation by promoting the ring-opening reactions of initial aromatic compounds in raw sludge and inhibiting the polymerization of open-chain intermediate products. The addition of NaOH + H2O2or Ni + H2O2as combined catalysts significantly lowered PAH concentrations while increasing the H2yield. The addition of 5 wt% Ni + H2O2reduced PAH concentrations in the liquid and solid residues by 70% and 44%, respectively, while the H2yield escalated from 0.13 mol/kg OM to 3.88 mol/kg OM. Possible mechanisms associated with the reaction pathways of these combined catalysts are proposed.
研究领域:
环境科学
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化学工程
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DOI
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 4
·e202405084
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IF 2
Yaqi Kong,Xinjin Zhang,Chongchao Zhang,Ziyin Yang
Graphical In this work, nitrogen and ruthenium nanoparticles were anchored on the inner and outer surfaces of graphene hollow nanospheres (N/GHSs/Ru) using a template method to create nanomaterials with dual active sites. Because of the synergistic effect of the superfine Ru nanoparticles and N, N/GHSs/Ru nanomaterials showed excellent electrochemical performance for H 2 O 2 detection. The efficient construction of active sites is crucial for regulating the electrocatalytic activity of nanomaterials. In this work, nitrogen and ruthenium nanoparticles were anchored onto the inner and outer surfaces of graphene hollow nanospheres (N/GHSs/Ru) via the template method. Notably, the synergistic effect of the superfine Ru nanoparticles and N enhances the electrocatalytic activity of the N/GHSs/Ru nanomaterials, exhibiting excellent electrochemical performance for H 2 O 2 detection. N/GHSs/Ru nanomaterials were characterized by transmission electron microscopy (TEM), energy dispersion spectroscopy (EDS), field emission scanning electron microscopy (FESEM), X-ray diffraction (XRD), and X-ray photoelectron spectrometry (XPS). The electrocatalytic reduction of H 2 O 2 showed a wide linear range from 0.02 to 5.6 mM, with a high sensitivity of 668.86 µA·mM −1 ·cm −2 and a limit of detection as low as 6.41 µM. This work offers a way to construct dual active sites, making N/GHSs/Ru as a promising material for nonenzymatic electrochemical H 2 O 2 sensors.
研究领域:
材料科学
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电化学传感器
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纳米技术
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DOI
高影响因子
ACTA PHARMACOLOGICA SINICA
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2024
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IF 6.9
Zhang Chen-chun,Li Yuan,Jiang Chang-you,Le Qiu-min,Liu Xing,Ma Lan,Wang Fei-fei
The O-linked-β-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is a critical post-translational modification that couples the external stimuli to intracellular signal transduction networks. However, the critical protein targets of O-GlcNAcylation in oxidative stress-induced apoptosis remain to be elucidated. Here, we show that treatment with H 2 O 2 inhibited O-GlcNAcylation, impaired cell viability, increased the cleaved caspase 3 and accelerated apoptosis of neuroblastoma N2a cells. The O-GlcNAc transferase (OGT) inhibitor OSMI-1 or the O-GlcNAcase (OGA) inhibitor Thiamet-G enhanced or inhibited H 2 O 2 -induced apoptosis, respectively. The total and phosphorylated protein levels, as well as the promoter activities of signal transducer and activator of transcription factor 3 (STAT3) and Forkhead box protein O 1 (FOXO1) were suppressed by OSMI-1. In contrast, overexpressing OGT or treating with Thiamet-G increased the total protein levels of STAT3 and FOXO1. Overexpression of STAT3 or FOXO1 abolished OSMI-1-induced apoptosis. Whereas the anti-apoptotic effect of OGT and Thiamet-G in H 2 O 2 -treated cells was abolished by either downregulating the expression or activity of endogenous STAT3 or FOXO1. These results suggest that STAT3 or FOXO1 are the potential targets of O-GlcNAcylation involved in the H 2 O 2 -induced apoptosis of N2a cells.
研究领域:
细胞生物学
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分子生物学
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神经科学
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DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2025
·
IF 9
Lu Zhang,Haotian Wang,Ju Peng,Qijie Yi,Dan Zhang,Li Lv,Jianhao Qiu,Wenxiang Tang
An effective and environmentally friendly way to treat wastewater powered by solar energy is to combine photocatalytic purification technology with in-situ H 2 O 2 generation. This work developed a type II CdS/Bi 2 O 2 CO 3 heterojunction photocatalyst through a MOF-based pyrolysis strategy, which was further applied for simultaneous photodegradation of tetracycline and photocatalytic production of H 2 O 2 . The heterojunction generated by decorating CdS nanoparticles on Bi 2 O 2 CO 3 surface is characterized by close contact which results in efficient charge separation. It has been discovered that the CdS/Bi 2 O 2 CO 3 heterojunction may photocatalytically produce H 2 O 2 by a two-step O 2 reduction reaction in ambient air and pure water when exposed to visible light, with a yield of 745.2 μmol⸱L −1 . In comparison to pure CdS, where the yield was only 95.7 μmol⸱L −1 , this is a substantial improvement, further demonstrating the great improvement of this unique heterojunction. Additionally, 98 % of the tetracycline could be degraded in 2 h, and the CdS/Bi 2 O 2 CO 3 heterojunction’s H 2 O 2 production in a tetracycline solution reached 1889.4 μmol⸱L −1 . This study shows how metal–organic framework-derived materials may be used for photocatalysis and offers information on how water purification technology can be combined with environmentally friendly H 2 O 2 production.
研究领域:
环境科学
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材料科学
·
光化学
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DOI
高影响因子
TALANTA
·
2023
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IF 6.1
Shuyan Ruan,Ru Liu,Xi Chen,Qian Huang,Chongxin Xiao,Ruiyun You,Luqiang Huang,Yunzhen Liu,Jingbo Chen,Xiufeng Xiao,Qingqiang Lin,Yudong Lu
As a reactive oxygen species (ROS), excessive production of H 2 O 2 contributes to the development of several diseases such as, inflammation, cancer, and respiratory diseases. Supplementation with endogenous or exogenous antioxidants can scavenge ROS and reduce the oxidation of cellular molecules, thus alleviating the generation of diseases. Therefore, the determination of H 2 O 2 content and its antioxidant activity is of great importance in disease diagnosis and treatment. In this paper, a ratiometric SERS sensor with a flexible cellulose membrane was designed for quantitative detection of H 2 O 2 and assessment of antioxidant activity. First, gold seeds were reduced on bacterial cellulose membrane (BCM) and Au NPs were smoothly deposited on the bacterial cellulose membrane (BCM) using halides to reduce the reduction potential in the growth solution to form a flexible BCM@Au NPs SERS substrate. Afterwards, the oxidation of H 2 O 2 was used to convert 3-mercaptophenylboronic acid (3-MPBA) to the corresponding phenol form 3-hydroxyphenylethanol (3-HTP). The change of substance resulted in a good linear relationship between the intensity ratio corresponding to the two Raman shifts of 881 cm −1 and 995 cm −1 and the H 2 O 2 concentration with a detection limit of 0.0186 μM. This opens up a new method for the detection of H 2 O 2 with high sensitivity and accuracy. In addition, this SERS platform was successfully used for the determination of antioxidant activity. It is promising to be applied to disease diagnosis and efficacy evaluation.
研究领域:
分析化学
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生物医学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Xuheng Li,Feng Liu,Weifang Zhang,Hongbo Lu,Jing Zhang
Preoxidation of As(III) to As(V) is required for the efficient removal of total arsenic in the treatment of wastewater. In this work, the electro-Fenton oxidation of As(III) with a high efficiency was successfully achieved by using the system of the stainless steel net (SSN) coating with reduced graphene oxide (RGO@SSN) as the cathode and stainless steel net (SSN) as the sacrificial anode. The RGO@SSN was synthesized by electrophoretic deposition-annealing method. The carbon disorder and defects of RGO resulted from the remained oxygen-containing functional groups facilitated the electrocatalytically active sites for two-electron oxygen reduction reaction (ORR). A high concentration (up to 1000 μmol/L) of H 2 O 2 was in-situ produced through two-electron oxygen reduction reaction of electro-catalysis, and then served as the electro-Fenton reagent for the oxidation of As(III). HO generated by H 2 O 2 participating the electro-Fenton reaction or decomposed at the surface of RGO@SSN cathode at acid condition endowed the strong oxidizing ability for As(III). The electro-Fenton equipped with RGO@SSN cathode has a promising application in the oxidation and removal of organic or inorganic pollutants in wastewater.
研究领域:
环境科学
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材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
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2024
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IF 20.2
Rong Ji,Yuming Dong,Xinyu Sun,Chengsi Pan,Yunfan Yang,Hui Zhao,Yongfa Zhu
Nowadays, considerable attention has been directed towards the two-electron oxygen reduction for the photocatalytic H 2 O 2 production. However, the challenge of insufficient charge separation capacity markedly constrains the rate of H 2 O 2 production. Herein, we designed a donor-acceptor (D-A) interface characterized by rapid charge migration and separation to enhance the photocatalytic H 2 O 2 production. Specifically, naphthalenetetracarboxylic acid-diaminotriazole as an organic molecule has been judiciously chosen as the electron donor, while perylenetetracarboxylic acid serves as the electron acceptor. The novel semiconductor with D-A interface performs markedly strong internal electric field and demonstrates an excellent H 2 O 2 production rate of 3176 μM h −1 . The pronounced internal electric field facilitated by the D-A interface effectively expedites charge separation and induces the migration of photogenerated carriers to the O 2 reduction sites. This study introduces a novel paradigm for the design of D-A interfacial organic materials aimed at realizing enhanced photocatalytic capabilities.
研究领域:
材料科学
·
化学工程
·
环境科学
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产品关联
DOI
高影响因子
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