Metallomics
·
2023
·Vol. 15
·期 4
·
IF 3.4
Ma Junrong,He Man,Chen Beibei,Hu Bin
Thimerosal (THI) is widely used as an antimicrobial preservative, but can hydrolyze to ethylmercury, causing potentially neurotoxicity. In this work, a THP-1 cell line was used to investigate the biological behavior of THI. An on-line droplet microfluidic chip system combined with time-resolved inductively coupled plasma mass spectrometry was used to quantify Hg in single THP-1 cells. The cellular uptake and elimination behaviors of THI were studied, and the toxicity of THI in terms of redox balance was discussed. The results showed that a small number of cells (<5%) exhibited a high uptake content (>200 fg/cell) for THI, and most of the cells (68.8–85.8% for different exposure groups at 25 h) exhibited a relatively low uptake content (<20 fg/cell). After stopping exposure to THI, the cells showed an elimination process for Hg, which was rapid in the first several hours and gradually slowed down. When the elimination time was 25 h, 7.4–26.3% of the cells in different exposure groups still contained a detectable amount of Hg (>2 fg/cell), indicating Hg could not be eliminated completely, which may cause cumulative toxicity to macrophages. Moreover, it was found that exposure to THI even at 50 ng/mL can cause cellular oxidative stress behavior, leading to an increase in reactive oxygen species level and a decrease in glutathione level. This trend would continue for a period of time after stopping THI exposure. With the elimination of Hg, the redox balance of cells showed a tendency to stabilize and restore, but cannot be restored to normal status, indicating a long-term chronic toxicity of THI to THP-1 cells.
研究领域:
分析化学
·
细胞生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Current Pharmaceutical Analysis
·
2022
·Vol. 18
·期 2
·193-198
·
IF 0.743
Xu Bo-Yang,Zhu Si-Qi,Cheng Xian-Xin,Xu Yu,Liu Ji-Wu
Background: Compound Niuhuang Xiaoyan capsule is a realgar and cinnabar-containing traditional Chinese medicine, the contents of total arsenic and total mercury are found over regulation defined limits. However, only small amounts of arsenic in realgar and mercury in cinnabar are dissolved
in the gastric juice, so the determination of soluble arsenic and soluble mercury is more significant than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule. Objective: To study the contents of total arsenic, total mercury, soluble arsenic and soluble mercury
in Compound Niuhuang Xiaoyan capsule. Methods: Microwave digestion and semi-bionic extraction were used to pretreat the samples of Compound Niuhuang Xiaoyan capsule, and the inductively coupled plasma mass spectrometry method was established to determine the contents of total arsenic,
total mercury, soluble arsenic and soluble mercury in Compound Niuhuang Xiaoyan capsule. The accuracy of the established method was further evaluated by using a certified standard reference material prepared from dried citrus leaves (GBW10020(GSB-11)). Results: The spiked recoveries were
within 95-105 %. The R.S.D% of repeatability (N=6) and precision (N = 6) were below 5.0 %. The correlation coefficients of linear (R) for arsenic and mercury were both above 0.998. The limits of quantification (LOQ) were below 0.1 μg/L. The contents of As and Hg were defined in the dried
citrus leaves and very near to the standard values provided by the manufacturer. The established method was applied for the analysis of three batches of Compound Niuhuang Xiaoyan capsule produced by three different manufacturers successfully. Insufficient realgar or cinnabar was formulated
in some Compound Niuhuang Xiaoyan capsule samples. Conclusion: The analysis showed that the contents of soluble arsenic and soluble mercury in artificial gastric juice were significantly less than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule. The results
provided a reference for further study on the toxicology and pharmacokinetics of Compound Niuhuang Xiaoyan capsule.
研究领域:
中药学
·
分析化学
·
毒理学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Guangyang Xiao,Beibei Chen,Man He,Bin Hu
Avian influenza virus (AIVs), hosted in poultry, are the pathogens of many poultry diseases and human infections, which bring huge losses to the poultry breeding industry and huge panic to society. Therefore, it is of great significance to establish accurate and sensitive detection methods for AIVs. In this work, a dual-mode detection method based on immuno-rolling circle amplification (immuno-RCA) and quantum dots (QDs) labeling for inductively coupled plasma mass spectrometry (ICP-MS) and fluorescence detection of H9N2 AIV was developed. The dual-mode detection of the QDs by ICP-MS and fluorescence is used to achieve mutual verification within the analysis results, thus improving the accuracy of the method. With the immuno-RCA, the sensitivity of the method was increased by two orders of magnitude. The limit of detection of the proposed method is 17 ng L −1 and 61 ng L −1 , and the linear range of the proposed method is 0.05–5 ng mL −1 and 0.1–5 ng mL −1 with ICP-MS and fluorescence detection, respectively. The relative standard deviation (n = 7) is 4.9% with ICP-MS detection and 3.1% with fluorescence detection. Furthermore, the proposed method was applied to the analysis of chicken serum samples, no significant different was found for two modes detection and the recoveries of the spiking experiments are acceptable, indicating that the method has good practical potential for real sample analysis.
研究领域:
分析化学
·
病毒学
·
生物技术
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 3
·927-934
·
IF 4.58
Liu Xiaolan,Suo Feiya,He Man,Chen Beibei,Hu Bin
Monoliths composed of poly(1-vinylimidazole-ethylene glycol dimethacrylate), referred to as poly(VI-EGDMA), were modified with imidazole groups and used in capillary microextraction (CME) of trace levels of Co(II), Ni(II) and Cd(II) ions from human urine samples. The effects of sample pH, sample flow rate, sample volume, elution conditions and potentially interfering ions were investigated. Under the optimized conditions, the limits of detection (3σ) are 0.6 ng L −1 for Co(II), 6.4 ng L −1 for Ni(II) and 0.7 ng L −1 for Cd(II), with the relative standard deviations of 1.8 (Co), 4.2 (Ni) and 2.2% (Cd). A Certified Reference Material (ZK018–2, lyophilized human urine) was analyzed to validate the method, and the values found were in good agreement with the certified ones. The method was successfully applied to the analysis of trace Co, Ni and Cd in human urine samples, and the recoveries for spiked samples were in the range from 89% to 105%. The one-step preparation of monolithic material in our perception is an attractive feature for this material which also excels by good extraction performance in that it possesses abundant adsorption sites, good stability and a long lifetime.
Two poly(1-vinylimidazole-ethylene glycol dimethacrylate) monolithic capillaries were combined with ICP-MS to improve the analytical speed and used for the analysis of Co(II), Ni(II) and Cd(II) in human urine samples.
研究领域:
分析化学
·
医学检测
·
环境科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Xiaoting Li,Beibei Chen,Man He,Han Wang,Guangyang Xiao,Bin Yang,Bin Hu
In this work, we demonstrate a novel method based on inductively coupled plasma mass spectrometry (ICP-MS) detection with gold nanoparticles (Au NPs) and quantum dots (QDs) labeling for the simultaneous counting of two circulating tumor cell lines (MCF-7 and HepG2 cells) in human blood . MCF-7 and HepG2 cells were captured by magnetic beads coupled with anti-EpCAM and then specifically labeled by CdSe QDs-anti-ASGPR and Au NPs-anti-MUC1, respectively, which were used as signal probes for ICP-MS measurement. Under the optimal experimental conditions, the limits of detection of 50 MCF-7, 89 HepG2 cells and the linear ranges of 200-40000 MCF-7, 300-30000 HepG2 cells were obtained, and the relative standard deviations for seven replicate detections of 800 MCF-7 and HepG2 cells were 4.6% and 5.7%, respectively. This method has the advantages of high sensitivity, low sample consumption, wide linear range and can be extended to the simultaneous detection of multiple CTC lines in human peripheral blood.
研究领域:
分析化学
·
癌症研究
·
纳米技术
·
生物医学工程
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DOI
PubMed
高影响因子
Journal of Chemistry
·
2023
·
IF 3
Tong Chen,Wanjing Cui,Ziwei Bi,Yafei Guo,Guoliang Zhu,Xiuyun Pan,Zhiqiang Cui,Zhen Yang,Tianlong Deng
The analysis of iodine content in kelp is mainly focused on determining total iodine content, which lacks a rapid and sensitive analytical method for iodine species corresponding to iodate, iodide, monoiodotyrosine (MIT), and diiodo-tyrosine (DIT). Based on exploring the pretreatment methods of enzymatic hydrolysis of kelp, a rapid determination method of high-performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC-ICP-MS) for different iodine species existed in kelp samples was established. The pretreatment method is trypsin hydrolysis of kelp samples treated at 50°C for 10 hours, then the extraction solution was obtained by centrifugation, and filtration was proved for the first time. A high-efficiency separation anion-exchange column (Dionex IonPac AS-14) for iodate, iodide, MIT, and DIT with 0.1 mol·L−1(NH4)2CO3 solution at pH = 9 as a mobile phase, a flow rate of 0.8 mL·min−1 for the first 1500 s, and 1.2 mL·min−1 for the last 1800 s was confirmed. The linear ranges of the standard calibrations on four iodine species are 1.0–150 μg·L−1, and the detection limits are 1.62, 1.02, 1.59, and 2.55 μg·L−1, respectively, while the RSD values of four iodine species are all less than 2%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in kelp samples.
研究领域:
分析化学
·
环境科学
·
海洋生物学
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产品关联
DOI
高影响因子
TALANTA
·
2019
·
IF 4.916
Xiaoyu Jia,Junyi Zhao,Hongyun Ren,Jiani Wang,Zixiao Hong,Xian Zhang
Zwitterion-functionalized polymer microspheres (ZPMs) have been designed and used as the core adsorbent for the selective and rapid enrichment of mercury species in environmental waters by an on-line solid-phase extraction (SPE). Zwitterionic S-ZPMs and N-ZPMs having an anionic sulfonic group, but differing in cationic S + and N + moieties were prepared from Merrifield resin by treating with 1,2-ethanedithiol and imidazole , followed by modification with 1,3-propanesultone. Without any additional reagents, inorganic mercury (In-Hg), methylmercury (MeHg) and ethylmercury (EtHg) were quantitatively adsorbed on the home-made mini-column packed with ZPMs. Following extraction with 60 mM ammonium acetate and 0.15% L -cysteine ( L -Cys) solution within seconds, separation and speciation of mercury were successfully achieved by the HPLC–ICP-MS technique. Under the optimized experimental conditions, the enrichment factors (EFs) are up to 105 and 98 for inorganic and organic mercury only with 40 mL sample solution, respectively. The low detection limits of 0.78 ng L −1 , 0.63 ng L −1 , and 0.49 ng L −1 were reached for In-Hg, MeHg and EtHg, respectively, with the relative standard deviations (RSDs) below 4.1%. The feasibility and accuracy of the on-line SPE–HPLC–ICP-MS method were successfully applied for mercury speciation in surface water, seawater as well as certified reference material (CRM). The results here demonstrate a facile, sensitive and selective method for speciation of mercury at trace levels.
研究领域:
分析化学
·
环境科学
·
化工工程
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2023
·
IF 6.1
Chao Zhu,Qimin Liu,Yang Wang,Xiaolin Wang,Yuxuan Ma,Fangxia Yang,Wuzi Dong,Hong Ji
The production of selenium-enriched fish contributes to alleviating selenium deficiency for humans. In this study, selenium nanoparticles (SeNPs) comparable in bioavailability to selenomethionine (SeMet), increased SeMet content in O. macrolepis ( Onychostoma macrolepis ) muscle. Additionally, dietary SeNPs significantly enhanced selenocysteine (SeCys 2 ) and methylselenocysteine (MeSeCys) levels in O. macrolepis muscle. The effect of SeNPs on selenium speciation in grass carp muscle was consistent with O. macrolepis results. SeCys 2 and MeSeCys showed antioxidant capacity in HEK293T cells, indicating enhanced health benefits of Se-enriched fish produced using SeNPs. Furthermore, the addition of 0.3 mg/kg SeNPs significantly improved the flesh quality of O. macrolepis by reducing the content of crude fat and heavy metals, as well as increasing the levels of crude protein, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and the ratio of n-3/n-6 polyunsaturated fatty acids (PUFAs). Therefore, selenium-enriched fish produced from SeNPs is a good source for improving human dietary selenium intake.
研究领域:
水产养殖
·
营养学
·
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Heping Jiao,Ruixiang Bi,Fangli Li,Jingbo Chao,Guimin Zhang,Lihai Zhai,Ligang Hu,Zhenhua Wang,Caifeng Dai,Bing Li
The complex and cumbersome preparation of magnetic covalent organic frameworks (COFs) nanocomposites on a small scale limits their application. Herein, a rapid and easy route was employed for the preparation of magnetic thiourea-based COFs nanocomposites. COFs were coated on Fe 3 O 4 nanoparticles at room temperature without a catalyst within approximately 30 min. This method is suitable for the large-scale preparation of magnetic adsorbent. Using the as-prepared magnetic adsorbent (Fe 3 O 4 @COF-TpTU), we developed a simple, efficient, and sensitive magnetic solid-phase extraction-high performance liquid chromatography-inductively coupled plasma-mass spectrometry (MSPE-HPLC-ICP-MS) for the enrichment and determination of mercury species, including Hg 2+ , methylmercury (MeHg), and ethylmercury (EtHg). The effects of the experimental parameters on the extraction efficiency, including solution pH, adsorption and desorption time, composition and volume of the elution solvent, salinity, coexisting ions, and dissolved organic matter, were comprehensively investigated. Under optimised conditions, the limits of detection in the developed method were 0.56, 0.34, and 0.47 ng L −1 with enrichment factors of 190, 195, and 180-fold for Hg 2+ , MeHg, and EtHg, respectively. The satisfactory spiked recoveries (97.0–103%) in real water samples and high consistency between the certified and determined values in a certified reference material demonstrate the high accuracy and reproducibility of the developed method. The as-proposed method with simple operation, high sensitivity, and excellent anti-matrix interference performance was successfully applied to the enrichment and determination of trace levels of mercury species in the natural samples with complicated matrices, such as underground water, surface water, seawater and biological samples.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 24
·7849-7857
·
IF 3.978
Chang Liu,Chunyu Huang,Ruixue Ma,Wanying Zhai,Jingjing Deng,Tianshu Zhou
This work demonstrates a new strategy for sensing cerebrospinal acetylcholinesterase (AChE) as a cholinergic biomarker for brain dysfunction based on graphene quantum dot (GQD)-functionalized lanthanide infinite coordination polymer (Ln-ICP) nanoparticles. The ICPs used in this work were comprised of two components, i.e. a supramolecular Ln-ICP host formed by the coordination between the GMP ligand and central metal ion Tb3+, and guest GQDs with abundant functional groups, which were utilized as antenna ligands to further sensitize the fluorescence of Tb/GMP. Upon excitation at 300 nm, the obtained GQD@Tb/GMP ICP nanoparticles exhibited enhanced green fluorescence from Tb/GMP. With the addition of Cu2+, the competitive coordination between Cu2+ and GQDs weakened the antenna effect, leading to a decrease in the fluorescence of GQD@Tb/GMP ICPs. However, in the presence of thiocholine (TCh), a thiol-containing compound hydrolyzed from acetylthiocholine (ATCh) by AChE, a stronger coordination interaction between Cu2+ and TCh occurred, resulting in the restoration of the fluorescence of GQD@Tb/GMP ICPs. Using the method established herein, the cerebrospinal AChE fluctuation of rats with acute organophosphorus pesticide (OP) poisoning or chronic Alzheimer's disease (AD) could be monitored. This study essentially provides a novel approach to realize the direct monitoring of a biomarker for brain dysfunction by regulating the competitive coordination interaction reversibly, which is critical in the early diagnosis and therapy of brain diseases.
研究领域:
纳米技术
·
生物化学
·
神经科学
·
分析化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2022
·
IF 12.545
Xueqin Huang,Zhubao Zhang,Lingzhi Chen,Yongjian Lin,Runmin Zeng,Jun Xu,Shanze Chen,Jianglin Zhang,Huaihong Cai,Haibo Zhou,Pinghua Sun
Ultra-sensitive detection of pathogenic bacteria is of great significance in the early stage of bacterial infections and treatment. In this work, we report a novel strategy using multifunctional Au nano-bridged nanogap nanoparticles (Au NNPs)-based sandwich nanocomposites , that made of Concanavalin A-conjugated Fe 3 O 4 @SiO 2 NPs (ConA-Fe 3 O 4 @SiO 2 NPs)/bacteria/aptamer-modified Au NNPs (apt-Au NNPs), for bacteria discrimination and quantitative detection by surface-enhanced Raman scattering (SERS) and inductively coupled plasma mass spectrometry (ICP-MS), and subsequently photothermal antibacterial assay. The sandwich nanocomposite consists of ConA-Fe 3 O 4 @SiO 2 NPs to magnetically enrich and photothermal killing bacteria , and dual-signal tags of apt-Au NNPs for both SERS sensing and ICP-MS quantification. This strategy can specifically distinguish different kinds of pathogenic bacteria, and provided a good linear relationship of Staphylococcus aureus ( S. aureus ) in the range from 50 to 10 4 CFU/mL with a detection limit of 11 CFU/mL, as well as realized ultralow amounts of bacterial detection in serum sample with high accuracy. Based on the quantitative detection, high antibacterial efficiency was monitored by ICP-MS. Overall, the established method combines bacteria discrimination, quantitative detection, and photothermal elimination with a simple and rapid process, which provides a novel way for the early diagnosis and treatment of bacterial infection.
研究领域:
纳米技术
·
分析化学
·
生物医学工程
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Qiao Huang,Ying Liu,Lei Tian,Fuqiang Xiong,Zongjun He,Yanhui Zhao,Shuangquan Xiang,Xianping Qiu,Jianshen Yu,Tongwei Guan
By examining and analyzing bran-free fermented Baijiu (BFB) with varying storage periods (0–20 years), it was observed that the overall concentration of volatile compounds initially increases and subsequently decreases over time. Furthermore, BFB exhibited more kinds of long chain esters, higher concentration of acetals, and reduced furfural content. The process of cellaring can enhance the aged, sweet, and fruity aroma of BFB. 16 flavor compounds, including 1,1-diethoxyethane, ethyl dodecanoate, and ethyl hexadecanoate, can be used as markers for vintage BFB, and electronic sensory technology was capable of discerning BFB in different years. The results of redundancy analysis (RDA) showed a positive correlation between metals and aldehydes, esters, and ketones, while indicating a negative correlation with acids and alcohols. Al, Fe, and Ca underwent the most significant changes during storage period, and they were positively correlated with differential substances, such as benzaldehyde, vanillin, ethyl isovalerate, and ethyl palmitate ( P < 0.01).
研究领域:
食品科学
·
化学工程
·
分析化学
·
感官科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）