BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Xueqin Huang,Zhubao Zhang,Lingzhi Chen,Yongjian Lin,Runmin Zeng,Jun Xu,Shanze Chen,Jianglin Zhang,Huaihong Cai,Haibo Zhou,Pinghua Sun
Ultra-sensitive detection of pathogenic bacteria is of great significance in the early stage of bacterial infections and treatment. In this work, we report a novel strategy using multifunctional Au nano-bridged nanogap nanoparticles (Au NNPs)-based sandwich nanocomposites , that made of Concanavalin A-conjugated Fe 3 O 4 @SiO 2 NPs (ConA-Fe 3 O 4 @SiO 2 NPs)/bacteria/aptamer-modified Au NNPs (apt-Au NNPs), for bacteria discrimination and quantitative detection by surface-enhanced Raman scattering (SERS) and inductively coupled plasma mass spectrometry (ICP-MS), and subsequently photothermal antibacterial assay. The sandwich nanocomposite consists of ConA-Fe 3 O 4 @SiO 2 NPs to magnetically enrich and photothermal killing bacteria , and dual-signal tags of apt-Au NNPs for both SERS sensing and ICP-MS quantification. This strategy can specifically distinguish different kinds of pathogenic bacteria, and provided a good linear relationship of Staphylococcus aureus ( S. aureus ) in the range from 50 to 10 4 CFU/mL with a detection limit of 11 CFU/mL, as well as realized ultralow amounts of bacterial detection in serum sample with high accuracy. Based on the quantitative detection, high antibacterial efficiency was monitored by ICP-MS. Overall, the established method combines bacteria discrimination, quantitative detection, and photothermal elimination with a simple and rapid process, which provides a novel way for the early diagnosis and treatment of bacterial infection.
研究领域:
纳米技术
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分析化学
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生物医学工程
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微生物学
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产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2024
·
IF 6.5
Qiao Huang,Ying Liu,Lei Tian,Fuqiang Xiong,Zongjun He,Yanhui Zhao,Shuangquan Xiang,Xianping Qiu,Jianshen Yu,Tongwei Guan
By examining and analyzing bran-free fermented Baijiu (BFB) with varying storage periods (0–20 years), it was observed that the overall concentration of volatile compounds initially increases and subsequently decreases over time. Furthermore, BFB exhibited more kinds of long chain esters, higher concentration of acetals, and reduced furfural content. The process of cellaring can enhance the aged, sweet, and fruity aroma of BFB. 16 flavor compounds, including 1,1-diethoxyethane, ethyl dodecanoate, and ethyl hexadecanoate, can be used as markers for vintage BFB, and electronic sensory technology was capable of discerning BFB in different years. The results of redundancy analysis (RDA) showed a positive correlation between metals and aldehydes, esters, and ketones, while indicating a negative correlation with acids and alcohols. Al, Fe, and Ca underwent the most significant changes during storage period, and they were positively correlated with differential substances, such as benzaldehyde, vanillin, ethyl isovalerate, and ethyl palmitate ( P < 0.01).
研究领域:
食品科学
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化学工程
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分析化学
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感官科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2019
·
IF 5.399
Danyu Zhang,Shiwei Yang,Qingfang Ma,Jiannan Sun,Heyong Cheng,Yuanchao Wang,Jinhua Liu
This work establishes a hyphenated methodology coupling HPLC with ICP-MS for simultaneous speciation analysis of arsenic, mercury and lead for the first time. Four arsenicals (As(III), DMA, MMA and As(V)), four mercurials (Hg(II), MeHg, EtHg and PhHg) and three lead compounds (Pb(II), TML and TEL) were simultaneously analyzed within only 8 min with acceptable resolution (2.0–8.2 for As, 1.6–6.1 for Hg and 2.7–4.0 for Pb). The detection limits were 0.036–0.20 for As-species, 0.023–0.041 for Hg-species, and 0.0076–0.14 μg L −1 for Pb-species. The developed method was applied for the measurement of five lotus seed samples, indicating the presence of DMA (19.6–28.2 μg kg −1 ), TML (1.4–2.9 μg kg −1 ), MeHg (1.2–4.8 μg kg −1 ) and EtHg (0.8–2.2 μg kg −1 ). This method provides a promising tool for studying the toxic, metabolic and bioavailable behaviors of arsenic, mercury and lead.
研究领域:
分析化学
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环境科学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yong-Hai Yuan,Feng Yang,Qiu Zhang,Qinghong Xu,Meilan Wen
This study focuses on comparing the performance of pneumatic nebulization (PN), hydride generation (HG), and photochemical vapor generation (PVG) sampling modes coupled with inductively coupled plasma mass spectrometry (ICP-MS) for the determination of Se and Te in geological samples. ICP-MS operating parameters for each sampling mode, such as RF power, flow rate of collision gas, and sampling depth, were optimized. The introduction efficiency of Se and Te in PN mode was about 4.71% and 4.58% respectively, with the best reproducibility. HG mode had the highest introduction efficiency (57.01% for Se and 53.02% for Te), but the poorest reproducibility. PVG mode showed a good balance between efficiency and reproducibility, with introduction efficiencies of 45.38% for Se and 38.84% for Te, and lower limits of detection (LODs) of 0.001 μg·L-¹ for both Se and Te. Analysis of 14 geological certified reference materials (CRMs) indicated that for samples with Se > 0.1 μg·g-¹ and Te > 0.05 μg·g-¹, both PN and PVG sampling modes can obtain satisfactory results, and PN mode is more convenient. For samples with Se ≤ 0.1 μg·g-¹ and Te ≤ 0.05 μg·g-¹, HG or PVG sampling modes are recommended after enrichment pretreatment. This research provides a more precise and reliable method selection basis for analyzing Se and Te in geological samples at different concentration levels.
研究领域:
分析化学
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地球化学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Sciences
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2020
·
IF 4.302
Zhao-Feng Yuan,Williamson Gustave,Raju Sekar,Jonathan Bridge,Jia-Yue Wang,Wei-Jia Feng,Bin Guo,Zheng Chen
The redox-sensitive elements, such as iron, manganese, sulfur, phosphorus, and arsenic, shift their speciation every millimeter (mm) across the soil-water interface in the flooded soil environments. Monitoring of element speciation at this high-resolution (HR) within the SWI is still difficult. The key challenge lies in obtaining sufficient porewater samples at specific locations along the soil gradient for downstream analysis. Here with an optimized inductively coupled plasma mass spectrometry (ICP-MS) method and a HR porewater sampler, we demonstrate mm-scale element profiles mapping across the SWI in paddy soils. High-concentrations of iron and manganese (> 10 mg/L) were measured by ICP-MS in an extended dynamic range mode to avoid signal overflow. The iron profile along the SWI generated by the ICP-MS method showed no significant difference ( p < 0.05) compared to that measured independently using a colorimetric method. Furthermore, four arsenic (arsenite, arsenate, monomethylarsonic and dimethylarsinic acid), two phosphorus (phosphite and phosphate) and two sulfur (sulfide and sulfate) species were separated in 10 min by ion chromatography -ICP-MS with the NH 4 HCO 3 mobile phase. We verified the technique using paddy soils collected from the field, and present the mm-scale profiles of iron, manganese, and arsenic, phosphorus, sulfur species (relative standard deviation < 8%). The technique developed in this study will significantly promote the measurement throughput in limited samples (e.g. 100 μL) collected by HR samplers, which would greatly facilitate redox-sensitive elements biogeochemical cycling in saturated soils.
研究领域:
土壤学
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环境科学
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分析化学
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DOI
PubMed
高影响因子
Analytical Methods
·
2012
·Vol. 4
·期 10
·3471-3477
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IF 1.547
Xu Han,Lianghui Cao,Heyong Cheng,Jinhua Liu,Zigang Xu
Ion-pair reversed phase high performance liquid chromatography coupled to inductively coupled plasma mass spectrometry (RP-HPLC-ICP-MS) has been used to determine two inorganic iodine species (I− and IO3−) and two organic iodinated amino acids (MIT and DIT) in seaweed and seawater samples within 5 min. The mobile phase used for a reversed phase C18 column was as follows: 2.0 mM tetraethylammonium hydroxide, 10 mM L-phenylalanine and 1% methanol (pH 8.9). Values of 1.5 mL min−1 for the flow rate of the mobile phase and 25 °C for the column temperature were selected for rapid separation of iodine species. The detection limits for IO3−, I−, MIT and DIT were 0.061, 0.052, 0.079 and 0.24 μg L−1, each, and the repeatabilities of peak height and peak area (20 μg L−1 for iodide and iodate, 40 μg L−1 for MIT, 100 μg L−1 for DIT) were all lower than 4%. Under the optimized conditions, the proposed method was successfully applied for the speciation analysis of iodine in seaweed and seawater samples. Recoveries of IO3−, I−, MIT and DIT in seaweed and seawater samples were between 90 and 110%. All results prove its advantages including high sensitivity, environmentally friendly, low time consumption, which are beneficial for routine analysis of iodine speciation in environmental and food fields.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
高影响因子
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
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2014
·Vol. 30
·期 12
·3245-3250
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IF 1.353
Guo Xuena,Liu Wei,Bai Xuejing,He Xiuping,Zhang Borun
High-performance liquid chromatography was used to separate Cr(III) and Cr(VI) in samples with detection by inductively coupled plasma mass spectrometry(ICP-MS). The separation was achieved on a weak anion exchange column. The mobile phase was pH 7.0 ammonium nitrate solution. The redox reaction between Cr(III) and Cr(VI) was avoided during separation and determination. This separation method could be used to separate the samples with large concentration differences between Cr(III) and Cr(VI). The alkaline digestion was used to extract chromium in solid sample, which had no effect on the retention time and the peak area of the Cr(VI). However, the conversion of Cr(VI) from Cr(III) was observed during alkaline digestion, which displayed positive relation with the ratio of Cr(III) and Cr(VI) in samples. Both Cr(III) and Cr(VI) contents of chromium yeasts cultured in media with different chromium additions were determined. The spike recoveries of Cr(VI) for chromium yeasts were in the range of 95–108 %.
研究领域:
分析化学
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环境科学
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生物化学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Kesheng Hu,Linlin Zhang,Haolin Zhou,Yuankun Liu,Shuying Liu,Beijia Chang,Xiaoqing Gao,Junqiang Yang,Keliang Shi,Xiaolin Hou
Selenium is a crucial trace element for antioxidant defense and immune function, with its health benefits varying significantly based on its chemical form. Tea, a widely consumed beverage, holds potential as a significant dietary source of selenium. However, the variations in selenium content and speciation in tea, influenced by cultivation and processing methods, have not been thoroughly studied, nor has the effect of brewing conditions on selenium release. This work addresses these gaps by developing a method utilizing inductively coupled plasma mass spectrometry to accurately quantify total selenium content and its speciation in tea samples. We optimized microwave digestion conditions to establish a rapid and efficient protocol for measuring total selenium and evaluated different brewing conditions to determine their impact on selenium extraction, identifying optimal parameters to maximize selenium intake from tea infusions. Additionally, AG1 × 4 ion exchange resin was employed to separate inorganic from organic selenium, and a method to extract and purify selenium bound to key tea components such as polyphenols, polysaccharides, and proteins was developed, with the extraction parameters optimized. Our findings provide a thorough understanding of selenium speciation in tea and highlight the importance of brewing conditions in enhancing selenium bioavailability, offering valuable insights for the development of selenium-enriched teas and advancing healthier dietary practices.
研究领域:
食品科学
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分析化学
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营养学
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Manlu Wang,Juan He,Jin Luo,Jing Hu,Xiandeng Hou
The determination of inorganic arsenic species in real samples can be particularly challenging due to their trace levels and the interferences arising from sample matrix. Normally, the speciation analysis necessitates chromatographic separation. Herein, we report a novel method for the ultrasensitive detection and non-chromatographic speciation of inorganic arsenic by inductively coupled plasma mass spectrometry (ICP-MS), utilizing CdS/MIL-100(Fe) composites as an adsorbent and photocatalyst. The synthesized CdS/MIL-100(Fe) could completely adsorb As(V) and As(III) within 5 and 105 min, respectively. Following filtration and re-suspension in formic acid, the adsorbed As(III)/As(V) were reduced to arsine (AsH 3 ) under UV irradiation and swept to ICP-MS for detection. The limits of detection were found to be 1.7 ng L −1 (without preconcentration) and 0.11 ng L −1 (after 20-fold preconcentration). The method was successfully applied to the determination of trace inorganic arsenic in various food and water samples.
研究领域:
分析化学
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环境科学
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食品科学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2025
·
IF 0
Qin Chuan,Jiao Yanni,Yang Luping,Wang Lin,Kong Zhengqiao,Jiang Dafeng,Zhang Jiacheng,Zhang Tianliang,Gao Xibao
Arsenic species were quantified to further explore the biotransformation of arsenic and better explain the potential health risks. In this research, the levels of six arsenic species (AsB, AsC, MMA, DMA, As 3+ , and As 5+ ) were determined in 160 squid samples by HPLC-ICP-MS. AsB was the most predominant speciation in squid tissues. The arsenic species, including AsB, AsC, and MMA, were found in all samples, followed by DMA (49.38%), As 3+ (21.25%), and As 5+ (2.5%). Results indicated squid samples from offshore had higher levels of AsB, AsC, MMA, and DMA. Pearson’s correlation analysis found correlations between As-MMA, DMA-As 5+ , and AsB-MMA, which showed a clear methylation pathway. The levels of inorganic arsenic were below the standard, and no potential health risks were identified. Therefore, the application of total arsenic in our previous study to assess health risks may be biased. In future, there is a need to optimize food safety monitoring of arsenic and comprehensively grasp the basic status and change law of marine ecological environment.
研究领域:
食品安全
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环境科学
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毒理学
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产品关联
DOI
PubMed
MICROCHEMICAL JOURNAL
·
2018
·
IF 2.746
Huimin Yu,Huan Du,Li Wu,Ruolin Li,Qun Sun,Xiandeng Hou
The speciation analysis of arsenic (As) has always received great attention, in which the analysis of complex matrix samples remains challenging, mainly manifested in selection of suitable sample pretreatment methods and sensitive analytical spectrometers. In this work, microwave-assisted extraction of total As and various As species in bones, with their respective determination by ICP-MS and HPLC-ICP-MS, was studied, and was successfully utilized to determine As species in panda and pig bones. The results reveal the content of As ranging from 28 to 75 μg g −1 in the panda bone samples and 0.27 μg g −1 in pig bone, in which >90% is inorganic As V except for little As III and organic As. The method could be applicable to the analysis of diverse bone samples.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Xiao Chaoqiang,Zhu Li,Zhang Xia,Gao Rumeng,He Shuwang,Lv Zhihua,Hu Changqin
Calcium carbonate which is extracted from the Earth in combination with other mineral impurities, is largely used in preparations for pediatric supplements. Elemental impurities in drug products pose toxicological concerns without therapeutic benefits. Thus, it is very urgent to assess the safety of chronic exposure to elements that may be present in trace amounts. In the present study, we developed high throughput ICP-MS method for the quantitative determination of 62 elemental impurities in high matric calcium carbonate samples and validated according to USP 233. Calcium carbonate preparations which state clearly used for child (including neonates, infants, toddlers and children) from 9 manufactures and two types of raw materials (light calcium carbonate and ground calcium carbonate) were investigated in terms of the content and variability of 62 elemental impurities. According to the results, ground calcium carbonate was more suitable to be used in pediatric preparations concerning elemental impurities. Parts of elemental impurities in CaCO3 preparations which are derived from the raw materials and the preparation process, may cause potential risks for children. These results indicate that it is necessary to establish a modern instrumental analysis method to evaluate and control elemental impurities in CaCO3 raw materials and preparations.
研究领域:
药学
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毒理学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）