INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Yuxiang Tang,Zeliang Wu,Hang Hu,Dianwen Yu,Chaohong Liu,Huiwen Jiang,Wenjing Luo,Heng Mei,Rong Xu,Yu Hu
Effective EPR and tumor penetration are bottlenecks in current nanomedicine therapy. Comosol software was utilized to analyze the motion process of nanoparticles (NPs) with different shapes, from blood vessels to tumor tissue, to address this. By calculation, urchin-like NPs experienced higher drag forces than spherical NPs, facilitating their EPR and tumor penetration effects. Thus, urchin-like indocyanine green-loaded hydroxyethyl starch-cholesterol (ICG@HES-CH) NPs were prepared by leveraging the instability of ICG responding to near-infrared light (NIR). Upon NIR exposure, ICG degraded and partly disintegrated ICG@HES-CH NPs, and its morphology transformed from spherical to urchin-like. Vincristine (VC), as a model drug, was loaded in urchin-like ICG@HES-CH NPs for the treatment of lymphoma. A20 lymphoma cells and 3T3-A20 tumor organoids were employed to investigate the influence of shape on NPs' cellular uptake, penetration pathway, and cytotoxicity. It demonstrated that urchin-like ICG@HES-CH NPs mainly transport across the extracellular matrix through intercellular pathways, easily reaching the deep tumor sites and achieving higher cytotoxicity. In vivo VC distribution and anti-tumor results indicated that urchin-like NPs increased VC EPR and penetration ability, lowering VC neurotoxicity and superior anti-tumor effect. Therefore, urchin-like ICG@HES-CH NPs have great translational potential to be used as chemotherapeutic nanocarriers in anticancer therapy.
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高影响因子
Nanoscale
·
2019
·Vol. 11
·期 12
·5717-5731
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IF 6.97
Ruihao Yang,Mengmeng Hou,Ya Gao,Lei Zhang,Zhigang Xu,Yuejun Kang,Peng Xue
Hollow mesoporous structures with interior cavities and expanded surface area have attracted considerable interest as drug delivery systems. In this study, a multifunctional nanotheranostic agent was developed by conjugating indocyanine green (ICG) and loading doxorubicin (DOX) onto the surfaces or within the cavities of hollow mesoporous Prussian blue (HMPB) nanoparticles, known as HMPB@PEI/ICG/DOX or simply HPID NPs, which were investigated as phototheranostic agents for in vivo fluorescence imaging and light-induced chemotherapy, photothermal therapy (PTT) and photodynamic therapy (PDT). These original HPID NPs exhibited strong near infrared (NIR) absorbance, reactive oxygen species (ROS) yield, and controlled chemotherapeutic drug release behavior. After intravenous injection of HPID NPs, highly efficient solid tumor ablation effects were observed in 4T1 tumor-bearing mouse models under NIR laser irradiation. Additionally, there was insignificant low-term toxicity or damage to normal tissues, as evidenced by histopathological and hemocompatibility analyses, suggesting that this agent has reliable biosafety for systemic applications. Taken together, the results of this study suggest that HPID NPs can produce tumor-specific and stimuli-triggered theranostic effects under tri-modal combination therapy. These HPID NPs advantageously provide traceable accumulation and activation and therefore could be a capable mediator in nanomedicines for eliminating solid tumors.
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RSC Advances
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2018
·Vol. 8
·期 58
·33198-33207
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IF 2.936
Yan Li,Wenqi Huang,Chunyan Li,Xiaoteng Huang
Herein, a multifunctional traceable and ultrasound-responsive drug targeted delivery system based on indocyanine green (ICG) and folic acid (FA) covalently conjugated lipid microbubbles (ILMBs–FA) is proposed. After encapsulation of the anticancer drug resveratrol (RV), the composite (RILMBs–FA) with fluorescence and ultrasound imaging capacity was studied for highly sensitive dual-imaging guided tumor targeted therapy. The resulting RILMBs–FA with an average particle size of 1.32 ± 0.14 μm exhibited good stability and biocompatibility characteristics. The RILMBs–FA featured a high RV loading ratio and the encapsulated RV has been demonstrated to be released from the microbubbles triggered by ultrasound (US) waves. In addition, it was found that the linked FA could facilitate a high cellular uptake of RILMBs–FA via the FA receptor-mediated endocytosis pathway. Compared to free RV and RILMBs, RILMBs–FA with US irradiation demonstrated a more significant tumor cell-killing efficacy mediated by apoptosis in vitro. Eight hours post intravenous injection of RILMBs–FA, the composites showed maximum accumulation in tumorous tissues according to in vivo fluorescence and US images. This ultimately led to the best tumor inhibition effect among all tested drugs under US irradiation. In vivo biosafety evaluations showed that RILMBs–FA featured high biocompatibility characteristics and no significant systemic toxicity over the course of one month. Taken in concert, these results demonstrate the versatility of this drug delivery system with dual-imaging and ultrasound-triggered drug release characteristics for potential future applications in cancer theranostics.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2020
·
IF 4.389
Qiang Hu,Kesi Wang,Liyan Qiu
Clinical extensive application of indocyanine green (ICG) is limited by several drawbacks such as poor bioenvironmental stability, aggregate propensity, and rapid elimination from the body, etc. In this study, we construct a novel amphiphilic mPEG-ACA-ICG conjugate by modifying synthetic heptamethine cyanine derivative ICG-COOH with a hydrophobic linker 6-aminocaproic acid (ACA) and amino-terminal poly(ethylene glycol) (mPEG-NH 2 ). The as-prepared mPEG-ACA-ICG conjugate has the ability to self-assemble into micellar aggregates in an aqueous solution with a lower CMC value than mPEG-ICG conjugate without ACA linker. More importantly, compared with free ICG and mPEG-ICG conjugate, mPEG-ACA-ICG nanomicelles exhibited better stability and higher photothermal conversion efficiency upon near-infrared light irradiation due to the intramolecular introduction of a hydrophobic ACA segment. In our in vivo experiment, mPEG-ACA-ICG nanomicelles ensured the formidable effect on tumor photothermal therapy (PTT) and the maximum tumor inhibition rate reached 72.6 %. In addition, real-time determination ability for fluorescence image-guided surgery (FIGS) of mPEG-ACA-ICG nanomicelles was also confirmed on tumor xenograft mice model. Taken together, mPEG-ACA-ICG conjugate may hold great promise for non-invasive cancer theranostics.
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Theranostics
·
2019
·
IF 8.063
Rui Wei,Guiying Jiang,MengQin Lv,Songwei Tan,Xueqian Wang,Ying Zhou,Teng Cheng,Xueqin Gao,Xi Chen,Wei Wang,Chenming Zou,Fei Li,Xiangyi Ma,Junbo Hu,Ding Ma,Danfeng Luo,Ling Xi
Metastasis is one of the most threatening aspects of cervical cancer. We developed a method to intraoperatively map the primary tumor, metastasis and metastatic sentinel lymph nodes (SLNs), providing real-time intraoperative guidance in cervical cancer. Methods : TMTP1, a tumor metastasis targeting peptide, was employed to modify the indocyanine green (ICG)-loaded poly (ethylene glycol)- poly (lactic-co-glycolic acid) (PEG-PLGA) micelles. The cervical cancer subcutaneous tumor model and lung metastasis model were established to determine the active targeting of ICG-loaded TMTP1-PEG-PLGA micelles (ITM) for the primary tumor and occult metastasis of cervical cancer. Human cervical cancer HeLa cells engineered by firefly luciferase were injected into the right hocks of BALB/c nude mice to develop the SLN metastasis model. The ITM and control ICG-loaded PEG-PLGA micelles (IM) were injected into the right hind footpads in the SLN metastasis model, and the migration and retention of micelles were recorded under near-infrared fluorescence. K14-HPV16 transgenic mice were also used to detect the image capability of ITM to target cancerous lesions. Results : ITM could actively target imaging of the primary tumor and cervical cancer metastasis. ITM quickly diffused from the injection site to SLNs along lymphatic capillaries and remained in the SLNs for 12 h. Moreover, ITM specifically accumulated in the tumor metastatic SLNs (T-SLNs), which could be successfully distinguished from normal SLNs (N-SLNs). Conclusion : ITM could achieve active targeting of the primary tumor, metastasis and T-SLNs, providing precise and real-time intraoperative guidance for cervical cancer.
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ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Haodong Cui,Dehong Hu,Jingnan Zhang,Guanhui Gao,Ze Chen,Wenjun Li,Ping Gong,Zonghai Sheng,Lintao Cai
Well-designed gold nanoclusters–indocyanine green nanoprobes (Au NCs–INPs) have been developed by the conjugation of Au NC assemblies with indocyanine green (ICG) for the therapeutic real-time monitoring based on fluorescence resonance energy transfer (FRET). The synthesized Au NCs–INPs demonstrated the improved cellular uptake and effective tumor targeting because of the enhanced permeability and retention effect and the gp60-mediated secreted protein acidic and rich in cysteine combined transport pathway, suggesting excellent dual-modal near-infrared fluorescence and photoacoustic imaging. Moreover, the simultaneous photodynamic therapy (PDT) and photothermal therapy (PTT) of Au NCs–INPs exhibited higher cancer cell killing and tumor removal efficiency than those of PDT or PTT alone. More importantly, a promising therapeutic monitoring strategy was performed based on FRET between Au NCs and ICG, suggesting that Au NCs–INPs could be utilized to evaluate the therapeutic response by real-time monitoring the change in Au NCs in fluorescence intensity together with ICG supersession. Therefore, Au NCs–INPs as a novel photosensitizer have great potentials for combined tumor imaging, therapy, and therapeutic monitoring in real time.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xuechun Wang,Yanxiang Sang,Hongjie Zhang,Benyan Zheng,Bibo Wang,Yuan Hu,Ruiying Wu
As a wound management strategy, this study developed a nanocomposite hydrogel based on the photothermal therapy and photodynamic therapy of nanomaterials, as well as the effects of Bletilla striata polysaccharide (BSP). In this research, MXene nanosheets loaded with indocyanine green were embedded into a hydrogel doped with BSP. By combining the photothermal effect and photodynamic effect, the treatment of wounds infected with Staphylococcus aureus was achieved under 808 nm near-infrared light irradiation (NIR). The characterization results of the hydrogel showed that it had good adhesiveness with an adhesive strength of 629 KPa ± 15.52 KPa and excellent biocompatibility; the swelling rate after 48 h was 485.53 % ± 0.26 %. In terms of antibacterial performance, the hydrogel had an antibacterial rate of over 90 % against Staphylococcus aureus . In addition, it could significantly promote the deposition of collagen and angiogenesis in infected wounds. When applied to wound treatment, the wound closure rate reached 98 % after 14 days, the collagen fraction of the healed wound was 87.63 % ± 1.81 %, and the epidermis thickness was 130.33 μm ± 1.25 μm. This indicated that the nanocomposite hydrogel dressing could effectively promote wound healing.
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COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
·
IF 5.4
Chenyang Qi,Guitao Tan,Haonan Hu,Yipin Zhang,Jie Chen,Qinqin Zhang,Jing Tu
Nanomaterial-based synergistic antibacterial agents are considered as promising tools to combat infections caused by antibiotic-resistant bacteria. Herein, multifunctional mesoporous silica nanoparticle (MSN)-based nanocomposites were fabricated for synergistic photothermal/photodynamic/chemodynamic therapy against methicillin-resistant Staphylococcus aureus (MRSA). MSN loaded with indocyanine green (ICG) as a core, while Prussian blue (PB) nanostructure was decorated on MSN surface via in situ growth method to form a core-shell nanohybrid (MSN-ICG@PB). Upon a near infrared (NIR) laser excitation, MSN-ICG@PB (200 μg mL −1 ) exhibited highly efficient singlet oxygen ( 1 O 2 ) generation and hyperthermia effect (48.7℃). In the presence of exogenous H 2 O 2 , PB with peroxidase-like activity promoted the generation of toxic hydroxyl radicals (•OH) to achieve chemodynamic therapy (CDT). PTT can greatly increase the permeability of bacterial lipid membrane , facilitating the generated 1 O 2 and •OH to kill bacteria more efficiently. Under NIR irradiation and exogenous H 2 O 2 , MSN-ICG@PB (200 μg mL −1 ) with good biocompatibility exhibited a synergistic antibacterial effect against MRSA with high bacterial killing efficiency (>98 %). Moreover, due to the synergistic bactericidal mechanism, MSN-ICG@PB with satisfactory biosafety makes it a promising antimicrobial agent to fight against MRSA.
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Science Bulletin
·
2022
·
IF 20.577
Simin Liang,Dehong Hu,Guofeng Li,Duyang Gao,Fei Li,Hairong Zheng,Min Pan,Zonghai Sheng
Focused ultrasound (FUS)-induced blood–brain barrier (BBB) opening is crucial for enhancing glioblastoma (GBM) therapies. However, an in vivo imaging approach with a high spatial–temporal resolution to monitor the BBB opening process in situ and synchronously is still lacking. Herein, we report the use of indocyanine green (ICG)-dopped microbubbles (MBs-ICG) for visualizing the FUS-induced BBB opening and enhancing the photothermal therapy (PTT) against GBM. The MBs-ICG show bright fluorescence in the second near-infrared window (NIR-II), ultrasound contrast, and ultrasound-induced size transformation properties. By virtue of complementary contrast properties, MBs-ICG can be successfully applied for cerebral vascular imaging with NIR-II fluorescence resolution of ∼168.9 μm and ultrasound penetration depth of ∼7 mm. We further demonstrate that MBs-ICG can be combined with FUS for in situ and synchronous visualization of the BBB opening with a NIR-II fluorescence signal-to-background ratio of 6.2 ± 1.2. Finally, our data show that the MBs-ICG transform into lipid-ICG nanoparticles under FUS irradiation, which then rapidly penetrate the tumor tissues within 10 min and enhance PTT in orthotopic GBM-bearing mice. The multifunctional MBs-ICG approach provides a novel paradigm for monitoring BBB opening and enhancing GBM therapy.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2022
·
IF 5.062
Jun Hong,Yating Tang,Mengting Zhou,Jing Deng,Hang Hu,Defeng Xu
Herein, polyethylene glycol-modified mesoporous polydopamine (PEG-MPDA) nanoparticles co-loaded with indocyanine green (ICG) and dimethylcurcumin (ASC-J9, DMC) were developed for combination therapy of castration-resistant prostate cancer. MPDA nanoparticles were prepared and surface modified with methoxypolyethylene glycol amine (mPEG-NH 2 , M.W. 2000 and 5000). The prepared PEG2000-MPDA and PEG5000-MPDA nanoparticles were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). ICG and DMC co-loaded PEG2000-MPDA and PEG5000-MPDA nanoparticles ( [email protected] &DMC and [email protected] &DMC) were prepared by a solvent diffusion method. The decrease in drug feeding ratios results in decline in drug loading content and increase in drug encapsulation rate. [email protected] &DMC and [email protected] &DMC nanoparticles with relatively high drug loading content (ICG 10.0 ± 0.5% and 9.2 ± 0.3%, DMC 6.7 ± 0.2% and 6.4 ± 0.3%) were selected for further studies. The hydrodynamic diameters of these two nanoparticles are between 200 and 300 nm . These two nanoparticles display enhanced stability of ICG in aqueous solution, near-infrared (NIR) laser irradiation-promoted DMC release, efficient photothermal effect, enhanced cellular uptake, and excellent biocompatibility . In in vitro antitumor effect study, these two nanoparticles exhibit enhanced antitumor effect against 22Rv1 cells as compared to ICG&DMC mixture upon 808 nm laser irradiation. Western blot analyses indicate that these two nanoparticles enhance the degradation of androgen receptor (AR) significantly. This work provides a novel strategy and an effective co-delivery system for combinational castration-resistant prostate cancer therapy.
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Nano Research
·
2018
·Vol. 11
·期 6
·3385-3395
·
IF 7.994
Wang Wei,Zhao Yang,Yan Bei-Bei,Dong Liang,Lu Yang,Yu Shu-Hong
Calcium carbonate-doxorubicin@silica-indocyanine green nanospheres with high uniformity and monodispersity were designed and synthesized, in order to provide a photo-triggered strategy for drug-resistant cancer therapy. Under near-infrared laser irradiation, the nanospheres transformed laser power into local heat and reactive oxygen species via the connected indocyanine green molecule, thus exhibiting photothermal and photodynamic effects. Moreover, the photo-triggered drug release based on calcium-assisted silica degradation was observed, endowing the nanospheres with chemotherapeutic properties. Finally, combined therapeutic effects against drug-resistant human breast cancer cells were successfully obtained. These photo-triggered materials based on calcium carbonate could provide a promising platform for enhanced multimodal cancer therapies.
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ADVANCED FUNCTIONAL MATERIALS
·
2015
·Vol. 25
·期 47
·7280-7290
·
IF 11.805
Kerong Deng,Zhiyao Hou,Xiaoran Deng,Piaoping Yang,Chunxia Li,Jun Lin
A novel nanoplatform based on tungsten oxide (W 18 O 49 , WO) and indocyanine green (ICG) for dual-modal photothermal therapy (PTT) and photodynamic therapy (PDT) has been successfully constructed. In this design, the hierarchical unique nanorod-bundled W 18 O 49 nanostructures play roles in being not only as an efficient photothermal agent for PTT but also as a potential nanovehicle for ICG molecules via electrostatic adsorption after modified with trimethylammonium groups on their surface. It is found that the ability of ICG to produce cytotoxic reactive oxygen species for PDT is well maintained after being attached on the WO, thus the as-obtained WO@ICG can achieve a synergistic effect of combined PTT and PDT under single 808 nm near-infrared (NIR) laser excitation. Notably, compared with PTT or PDT alone, the enhanced HeLa cells lethality of the 808 nm laser triggered dual-modal therapy is observed. The in vivo animal experiments have shown that WO@ICG has effective solid tumor ablation effect with 808 nm NIR light irradiation, revealing the potential of these nanocomposites as a NIR-mediated dual-modal therapeutic platform for cancer treatment.
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