SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Dongyan Hou,Yi You,Xiaohong Wu,Chen Li,Shan Wu,Cuiling Zhang,Yuezhong Xian
Fluoride ion (F − ) is an essential micronutrient in the human body, however, an abnormal large intake of F − can result in adverse effects, such as skeletal fluorosis or DNA damage. Therefore, it is of great significance to develop convenient assays for the visual detection of F − with high sensitivity. We rationally developed the nanosized iron-based metal organic frameworks (Fe-MOFs) by using 3,5-dicarboxybenzeneboronic acid as organic linkers and Fe 3+ as metal ions through the solvothermal method, which can be applied for detecting F − with high sensitivity and selectivity owing to the specific recognition of boronic acid groups towards F − and the strong binding affinity between F − and Fe 3+ . The Fe-MOFs possess excellent peroxidase-like activity, which can be effectively inhibited by F − . Moreover, a portable test kit for the determination of F − was developed by embedding Fe-MOFs into hydrogel, and the visual measurement of F − was realized based on smartphone by identifying the color signal of the oxidation of 3,3',5,5'-tetramethylbenzidine dihydrochloride. The output signal (R/G) was linear with F − concentration in the range of 10.0–200.0 μM. The Fe-MOFs-based colorimetric strategy is easy-operation, low-cost and equipment-free, which has been further applied to detect F − in the real water samples.
研究领域:
分析化学
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材料科学
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环境科学
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DOI
高影响因子
TALANTA
·
2025
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IF 6.1
Yun Wen,Li-Ping Wang,Yong-Liang Yu,Shuai Chen
Accurate and timely detection of ions and metabolites in urine is essential for health monitoring. However, traditional methods face challenges in integrating multiplexity, signal stability, and portability. To address these issues, this study developed a 3D-printed portable dual-mode gel microspheres kit. The hydrogel microspheres were prepared by encapsulating the respective indicators: Urine pH Indicator (UpHI) for pH detection, Urine Calcium Ion Indicator (UCaI) for Ca 2+ detection, and Urine Creatinine Indicator (UCrI) for creatinine detection. Each indicator was optimized for its specific target to ensure high sensitivity and selectivity. The system employs dual-mode UV and visible light analysis, combined with smartphone imaging and RGB data processing, ensuring rapid, intelligent, and high-throughput detection. The integration of gel microspheres with a 3D-printed microdevice enhances the portability and user-friendliness of the detection system. This design facilitates long-term storage and transportation while ensuring usability for non-technical individuals in home settings. The kit demonstrated excellent encoding capacity, maintained stable signals for up to a week, and achieved integrated detection, data acquisition, and analysis within approximately 6 min. The cost per microsphere is under $0.20, making it a cost-effective solution. In conclusion, this method offers an effective solution for portable and timely urine health monitoring in home settings and provides a promising approach for the future development of portable kits with expanded detection pathways.
研究领域:
医学诊断
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生物材料科学
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分析化学
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DOI
PubMed
高影响因子
MOLECULES
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2022
·Vol. 27
·期 15
·4689
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IF 4.927
Dan Wu,Qile Xia,Jianfeng Zhou,Xingqian Ye
The traditional method for the determination of protein in food needs the operations of digestion, distillation, absorption, and titration; therefore, it is complicated and time-consuming and requires professional personnel. Is there a more convenient and faster detection method that can directly determine the ammonium ions in protein digestion solution to obtain the protein content of food and avoid the distillation–absorption–titration process? The feasibility of water ammonium ion test kits for food protein rapid detection was discussed here. After digestion, the protein in food transforms into ammonium ions in the digestion solution. Because of the variety of food, there are many different inorganic ions left in the food digestion solution, and at the same time, digestion agents are added in the digestion process and become potential interference factors in ammonium determination. Therefore, the detection accuracy of ammonium test kits needs to be evaluated first, including their anti-interference ability. The standard curve of ammonium was established by the test kit. When the ammonium concentration was 0.00–2.50 mg/L, the absorbance at 620 nm was linearly related to the ammonium concentration, the determination coefficient R2was 0.9995, and the detection limit of this method was 0.01 mg/L. The influences of temperature, pH value, and reaction time on the test kit method were discussed. The precision was 0.90–3.33%; the repeatability was 1.71–4.86%; and the recovery rate of tap water, river water, and sea water was controlled within 90–103%. The anti-interference ability of the evaluated test kit was better than that of the national standard detection method. The test kit, combined with sample pretreatment and protein conversion formula, was used to detect protein in different types of food (milk powder, rice flour, wheat flour, soy, banana, milk, fish food, chicken food, and dog food). The results showed that there were no significant differences (ρ > 0.05) between the national method and the test kit method. The ammonium ion test kit method shortened the determination time and had higher sensitivity, showing its potential for the rapid determination of food protein.Keywords:ammonium ion test kit;protein;rapid determination
研究领域:
食品科学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2023
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IF 3.4
Lihong Guo,Wenlei Xie,Xiangxin Xu
BACKGROUND The exploration of a robust solid acid catalyst for the efficient hydrolysis of cellulose is a research hotspot due to the need for clean production and sustainable development. KIT-6 silica offers abundant three-dimensionally interconnected mesopores, which are expected to be superior to mesoporous structures with one- or two-dimensional channels due to faster diffusion of reactants and products during reaction. The catalytic conversion of microcrystalline cellulose is examined in detail over a functionalized KIT-6 catalyst prepared by the method of Zr addition and subsequent sulfation. RESULTS In terms of cellulose conversion and product yield, the activity of the catalysts followed the order of sulfated Zr-KIT-6 > Zr-KIT-6 > SO 4 2− -KIT-6 > KIT-6. Over the sulfated Zr-KIT-6 catalyst with a Si/Zr molar ratio of 7:1, a maximum cellulose conversion of 95.0% was achieved with glucose yield of 23.4%, 5-hydroxymethylfufural yield of 4.9% and levulinic acid yield of 11.7% under the optimized reaction conditions: reaction temperature of 200 °C, reaction time of 2 h, catalyst amount of 0.3 g, cellulose-to-water mass ratio of 1:1. CONCLUSION The sulfated Zr-KIT-6 catalyst possessed the strongest and richest Brønsted acid sites, which readily provided protons for the cleavage of β -1,4 glycosidic bonds in cellulose, thus conferring superior catalytic activity in cellulose hydrolysis. A plausible pathway at the initial stage of cellulose hydrolysis over the catalyst was proposed. © 2023 Society of Chemical Industry (SCI).
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高影响因子
Catalysis Science & Technology
·
2025
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IF 4.2
Yifan Zhang,Jiaying Liu,Shiwei Wang,Zhihao Yu,Haojian Zhang,Dong Wang,Lin Zhu,Chunzheng Wu,Hongbo Yu
Modifying noble metal catalysts with transition metal oxides (MO x ) can markedly enhance catalytic performance through synergistic effects, yet precise spatial colocalization of active metal and promoter phases remains challenging. Here, we report the synthesis of KIT-6 supported Pd-MO x (M = Fe, Co, Ni) hybrid nanostructures via in situ transformation of bimetallic PdM nanoparticles under controlled thermal conditions. The resulting Pd-MO x /KIT-6 catalysts displayed superior activity and selectivity in the selective hydrogenation of acetophenone to 1-phenylethanol compared with monometallic Pd/KIT-6. Among them, Pd-CoO x /KIT-6 (Pd/Co=1:1) achieved the highest performance, with 99.7% acetophenone conversion and 98.2% selectivity, and demonstrated broad applicability for hydrogenating diverse ketones. Structural and kinetic studies revealed that interfacial Pd-CoO x sites play a pivotal role: Pd domains efficiently dissociate H 2 , while CoO x species enhance acetophenone adsorption. This synergistic interface-driven mechanism provides a general and costeffective strategy for the selective hydrogenation of multifunctional substrates.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Shuang Su,Zhiyang Zhang,Jiadong Chen,Yan Chen,Xin Cao,Shuoyang Yan,Ranran Yu,Jie Shao,Xiaokun Wang,Jaebum Choo,Lingxin Chen
On-site detection of hydrogen sulfide (H 2 S), a toxic byproduct of microbial decomposition, is essential for environmental surveys, sewage tracing, and occupational safety. While existing gold nanorods (Au NRs)-based colorimetric methods offer portability and sensitivity, their practicality is hindered by the instability of enzymes and colloidal nanoparticles. Herein, we present an enzyme-free, portable H 2 S detection device using Au NRs powder, ensuring highly sensitive and reliable colorimetric detection while enhancing long-term applicability. The sensing strategy involves two steps: H 2 S microextraction and colorimetric assay. First, H 2 S is accumulated into a NaOH aqueous drop from a complex sample matrix via headspace single-drop microextraction (HS-SDME) using sulfamic acid. In the colorimetric assay, dissolved H 2 S reduces KIO 3 to I 2 in glycine-HCl buffer (pH 2.6), which etches Au NRs in the presence of Br - and CTA + , causing a blue shift in the plasmon band with a distinct color change. This method achieves a visual detection limit of 0.05 µM, approximately 10 times lower than the Methylene Blue method. The use of HS-SDME ensures excellent selectivity against co-existing ions (≥10-fold) and small organic molecules (100-fold). Validation with real samples yielded recoveries of 92.40 %-109.51 %, demonstrating reliability in environmental monitoring and river pollution tracing. To simplify detection, we developed a liquid-free test kit containing an H 2 S microextraction tube (sulfamic acid powder), a colorimetric assay tube (Au NRs powder and solid buffer), and a portable heating device. This enzyme-free sensor ensures long-term stability and practicality, advancing Au NRs-based sensors for environmental monitoring.
研究领域:
环境科学
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分析化学
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纳米技术
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传感技术
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水污染监测
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2024
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IF 5.3
Wan-Yi Ge,Wen-Pu Shi,Xue-Ting Wang,Huan Liang,Xu-Dong Deng,Liang-Liang Chen,Xiao-Qian Jin,Wei-Hong Guo,Da-Chuan Yin
Protein liquid–liquid phase separation (LLPS) is a significant process in biology and material sciences. Finding suitable conditions for protein LLPS is essential for its in-depth and systematic study. Here, we provide a mixed solution screening kit based on biomacromolecule crowding agents to find protein LLPS conditions. The screening kit consists of 96 chemical formulations, in which the biomacromolecule crowding agents are used as the key components. The aqueous solution of the target protein is mixed with each chemical agent in the kit and then placed in an incubator for some time. The LLPS conditions for the target protein can be found by examining the LLPS phenomena in the droplets. We used nine proteins as model target proteins—lysozyme, bovine serum albumin, bovine hemoglobin, ovalbumin, catalase, FUS, Aβ, Tau, and α-Synuclein—to evaluate the performance of the screening kit. We found that utilization of the screening kit is efficient and useful to find different conditions for LLPS of proteins. The screening results reveal two types of conditions: those that promote and those that inhibit LLPS. Both conditions are extremely valuable because those that promote LLPS can be used to do extensive studies on the LLPS processes of the proteins of interest, whereas those that inhibit LLPS can be utilized as leads for developing novel medications to treat LLPS-related disorders. Furthermore, novel applications for the kit were investigated by utilizing the screening products.
研究领域:
生物学
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材料科学
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生物物理学
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药学
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DOI
高影响因子
RENEWABLE ENERGY
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2019
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IF 5.439
Jian He,Hu Li,Yufei Xu,Song Yang
γ-Valerolactone (GVL) is an interesting bio-based platform molecule that is utilized as green solvent and a versatile building block for the synthesis of bio-fuels and chemicals. Herein, an investigation on the efficient production of GVL from biomass-based carbonyl compounds such as furfural, levulinic acid, and its esters using 2-propanol as H-donor and solvent over stable Zr-incorporated mesoporous silica (KIT-5) catalysts was presented. Both Lewis and Brønsted acid sites were generated by the introduction of Zr into KIT-5, and the acid density of the resulting Zr-KIT-5(Si/Zr) could be controlled by simply adjusting Si/Zr molar ratio . Among these bifunctional catalysts , Zr-KIT-5(10) showed superior catalytic performance in the production of GVL (>91% selectivity) from biomass-derived carboxides (ca. 94% conversion), which was demonstrated to positively correlate with its large amount of acidic sites and facile access of active sites to interconnected pores. Moreover, the spent catalyst held about 90% of its original activity in the sixth run. Due to the presence of Brønsted and Lewis dual acidic sites in Zr-KIT-5, the direct conversion of furfural to GVL was also permitted in a single pot via tandem reactions involving hydrogenation, ring-opening, secondary hydrogenation, and subsequent cyclization.
研究领域:
催化化学
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生物质能
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绿色化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION
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2015
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IF 1.206
Chuan Li,Shao-Ping Nie,Ke-Xue Zhu,Tao Xiong,Chang Li,Joshua Gong,Ming-Yong Xie
Lactobacillus plantarum, as a probiotic, has many functional properties in human intestinal tract. This study examined the effects of L. plantarum NCU116 on loperamide-induced constipation in a mouse model. Loperamide (5 mg kg−1) was injected subcutaneously to induce constipation. Animals were divided to five groups: normal group, constipation group, constipation plus three doses of L. plantarum NCU116 groups (NCU116-L, 107 CFU/mL; NCU116-M, 108 CFU/mL; NCU116-H, 109 CFU/mL; respectively). Mice were treated with the probiotic for 15 d to assess the anti-constipation effects. Fecal parameters, intestinal transit ratio and the production of fecal short chain fatty acids, histological of colon and immunohistochemical in colonic interstitial cells of Cajal (ICC) by c-kit were all improved in L. plantarum NCU116-treated mice as compared to the constipation group. These results demonstrate that L. plantarum NCU116 enhanced gastrointestinal transit and alleviated in mice with loperamide-induced constipation.
研究领域:
胃肠病学
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微生物学
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药理学
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DOI
PubMed
高影响因子
iScience
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2023
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IF 5.8
Yao Li,Zi-Yu Liang,Hai-Lin Wang
Summary Red blood cells supply the oxygen required for all human cells and are in demand for emerging blood-loss therapy. Here we identified N6-methyl-2′-deoxyadenosine (6mdA) as an agonist that promotes the hyperproliferation of burst-forming unit erythroid (BFU-E) progenitor cells. In addition, 6mdA represses the apoptosis of erythroid progenitor cells (EPCs). Combined use of with SCF and EPO enabled cultures of isolated BFU-E to be expanded up to 5,000-fold. Transcriptome analysis showed that 6mdA upregulates the expression of the EPC-associated factors c-Kit, Myb, and Gata2 and downregulates that of the erythroid maturation-related transcription factors Gata1, Spi1, and Klf1. Mechanistic studies suggested that 6mdA enhances and prolongs the activation of erythropoiesis-associated master gene c-Kit and its downstream signaling, leading to expansion and accumulation of EPCs. Collectively, we demonstrate that 6mdA can efficiently stimulate the EPC hyperproliferation and provide a new regenerative medicine recipe to improve ex vivo generation of red blood cells.
研究领域:
血液学
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分子生物学
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再生医学
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细胞生物学
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Zeng Ronghua,Zhuo Dali,Dong Xiaoyu,Tang Jiayuan,Liu Yuxin,Zhang Shu,Luo Hong,Xu Fengzhou,Zou Zhirong
Sulfide ions (S 2− ) are a hazardous pollutant present in our daily lives; thus, the accurate detection of S 2− is critical for human health. However, conventional analytical methods have the disadvantage of high costs, complex operations, and the need for laboratory analysis. In this work, a miniaturized analytical kit was established for rapid analysis of S 2− in water samples and food additives. In the presence of platinum nanoparticles (NPs), H 2 O 2 was decomposed to O 2 catalyzed by Pt NPs, resulting in a substantial increase in pressure inside the sealed reaction bottle. In the presence of S 2- , S 2− forms a Pt–S bond with Pt NPs and induces aggregation, thereby reducing the catalytic activity of Pt NPs, resulting in a decrease in O 2 production and a corresponding decrease in gas pressure. Thus, the analysis of S 2− was achieved by the measurement of gas pressure. S 2− concentrations were inversely proportional to the gas pressures within a linear range of 0.2–5 μg/mL. A portable analytical kit was fabricated for rapid analysis of S 2− in various samples, achieving recoveries of 90–109%, confirming its suitability as a potential analytical device for on-site environmental analysis and rapid food inspection. Graphical
研究领域:
环境分析
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食品安全
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分析化学
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纳米材料
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传感器技术
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2018
·Vol. 10
·期 22
·2604-2613
·
IF 2.073
Xianxin Qiu,Longjie Zhong,Ying Gan,Kaiqi Su,Shihui Hu,Ping Wang
Okadaic acid is a kind of fat-soluble diarrhetic shellfish poison existing extensively in shellfish. The rapid on site detection of shellfish contaminated by the toxin is a serious problem. Herein, a new method combining a kit with the Bionic e-Eye was developed for the rapid detection of okadaic acid on site. After optimization the kit reached a very low limit of detection, 0.19 μg L−1, and the linear detection range was 0.2–10 μg L−1. The cross reaction rate with dinophysistoxins 1 was 23.6%. The kit had a small cross reaction with other kinds of toxin like saxitoxin, gynotoxin 2 & 3 and pectenotoxins 2-b. The recovery range was about 75.78–102.56%, and the CVs were lower than 20%. A correlation analysis was conducted between the Bionic e-Eye and a microplate reader in PBS and shellfish backgrounds. The detection results of the real shellfish samples by the Bionic e-Eye exhibited great consistency with high performance liquid chromatography. The total time taken to perform the detection was less than 90 minutes. Above all, the method integrated kit with the Bionic e-Eye was very suitable for the rapid detection of okadaic acid on site.
研究领域:
食品科学
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分析化学
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毒理学
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DOI
高影响因子
危险品化学品经营许可证（带存储）