JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
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2025
·Vol. 113
·期 1
·e35528
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IF 3.4
Yifan Lu,Xiangxin Lou,Wenxin Wang,Ziting Yang,Haochen Yao,Jinglei Wu
Massive bleeding and bacterial infection of wounds may be life-threatening or even lead to death. Nowadays, gelatin-based hemostatic sponges have been widely used, but gelatin is not antibacterial and has poor structural stability. In this study, we mixed an antibacterial polypeptide, ε-poly-L-lysine (EPL), into gelatin. A gelatin/ε-poly-L-lysine (Gel/EPL) sponge with hemostatic and antibacterial functions was prepared by ultraviolet (UV) crosslinking lyophilized Gel/EPL composite. Scanning electron microscopy showed that the sponge had an interconnected porous structure. The incorporation of EPL increased the hydrophilicity and water absorption capacity of the Gel/EPL sponge. The sponge had better structural stability after UV crosslinking. The antibacterial assay showed that bacteria could not grow normally around the Gel/EPL sponge. The contact between blood components and the sponge initiated coagulation via exogenous pathway activation, and no hemolysis occurred. In addition, in vivo experiments confirmed that the sponge has a faster clotting time and lower blood loss. These findings show that the developed Gel/EPL sponge has great potential as a novel hemostatic agent that can quickly stop bleeding and fight bacterial infections.
研究领域:
生物材料学
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医学工程
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药剂学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yong Zhang,Hongyu Jing,Bo Meng,Xiaohong Qian,Wantao Ying
The facile enrichment of glycopeptides or glycoproteins poses great challenges for glycoproteomic research. In this study, a novel hydrophilic material, named zwitterionic hydrophilic L-cysteine derivatized straticulate-C 3 N 4 composites (LCAC), were synthesized and evaluated for the enrichment of N-glycopeptides. LCAC exhibited good biocompatibility, excellent hydrophilicity and selectivity, by virtue of the large surface of C 3 N 4 and the zwitterionic property offered by cysteine. LCAC demonstrated excellent performance for N-glycopeptide enrichment with the sensitivity of 0.033 fmol/µL, selectivity of 1:100, and high recovery rate (∼85%). The performance of LCAC was demonstrated by the identification of 35 N-glycopeptides from IgG, as well as capturing 1809 human urine N-glycopeptides corresponding to 876 N-glycoproteins. Comparing the LCAC with our developed phenylboronic acid functionalized material showed a certain complementary due to the different binding mechanism. The simple production and enhanced hydrophilic properties make the material a promising choice for glycoproteomics researches.
研究领域:
蛋白质组学
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分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2020
·
IF 5.065
Zhicheng Gao,Ying Li,Xunqiu Wang
In this study, the solubility of l -malic acid in three binary solvents (ethyl acetate + tetrahydrofuran , acetonitrile + tetrahydrofuran, butyl acetate + acetonitrile) at atmospheric pressure from T = (278.15 to 323.15) K was measured via a gravimetric method. The results show that in the temperature range studied, the solubility of l -malic acid in the selected solvent gradually increases with the increase of temperature. The solubility of l -malic acid increases with the increase of the mass fraction of tetrahydrofuran at the binary system (ethyl acetate + tetrahydrofuran, acetonitrile + tetrahydrofuran), the solubility of l -malic acid increases with The mass fraction of butyl acetate increases and decreases at the butyl acetate + acetonitrile system. In addition, the dissolution behavior of l -malic acid was explained using Hansen solubility parameter (HSP). Furthermore, the solubility data were correlated with using the modified Apelblat equation, the NRTL model and the newly proposed Apelblat-Sun model, the results have shown that the modified Apelblat equation has the best fitting effect. After research found, the hydrogen bonding force plays a key role in the solubility of l -malic acid. So solubility of l - resulted malic acid from the integrated effect of solute–solvent and solvent–solvent interactions. In addition, the van't Hoff equation was used to calculate and discuss all thermodynamic parameters of dissolution, Including standard Gibbs dissolution (Δ sol G o ), standard dissolution enthalpy (Δ sol H o ) and standard dissolution entropy (Δ sol S o ), Where Δ sol H o ，Δ sol S o ，Δ sol G o were all greater than 0, it have been shown that the dissolution process is driven by heat absorption and entropy. This study can provide basic thermodynamic data for the crystallization and industrial separation of l -malic acid.
研究领域:
化学工程
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物理化学
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DOI
高影响因子
CRYSTAL GROWTH & DESIGN
·
2022
·
IF 4.01
Liyu Liu,Zaiyong Zhang,Yinxiang Cheng,Yihua Jiang,Liye Lu,Jian-Rong Wang,Xuefeng Mei
L-menthol is one of the most important flavoring additives with abundant plant sources. The distinctive odor, cooling characteristic, and diversified biological activities attribute to its widespread application, especially on confections, oral-care commodities, over-the-counter drugs, and tobacco flavors. However, the manufacturing and storage of L-menthol face challenges due to its high volatility and low melting point. In this work, cocrystallization provides a new strategy to manage the stability problem of this important ingredient. Coformers of dietary sources were chosen based on a supramolecular synthon design, and six cocrystals of L-menthol were synthesized by either a solvent-mediated or solvent-free approach. These new solid forms could significantly increase the melting point and reduce the vapor pressure up to 1/45 of the pure L-menthol. Moreover, cocrystals present a high retention rate under storage conditions. The superior stability can be ascribed to diversified supramolecular cavities assembled by coformers, to which L-menthol molecules are restricted by hydrogen bonds.
研究领域:
食品科学
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材料科学
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化学
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产品关联
DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2015
·
IF 2.587
Yunchang Fan,Xing Dong,Yun Li,Yingying Zhong,Juan Miao,Shaofeng Hua,Yongchun Sun
In this work, several hydroxyl-functionalized ionic liquids (ILs) were synthesized and used to extract tryptophan from the aqueous phase. The experimental results indicated that the hydroxyl-functionalized ILs presented better extraction ability than the dialkylimidazolium-based ILs. The extraction efficiency was significantly influenced by the pH of the aqueous phase. Moreover, the cationic form of tryptophan preferred to transfer into the IL phase, thereby suggesting a cation-exchange extraction mechanism. Driving forces of the extraction processes included hydrogen bonding, hydrophobicity, π–π stacking, and the screening effect. Among these factors, hydrogen bonding was the main parameter, as suggested by the results of thermodynamic analysis. After extraction, [C1C6OHim]NTf2 could be reused by using the pH swing effect. These findings demonstrate that [C1C6OHim]NTf2 is a good alternative to conventional solvents for recovering tryptophan from the aqueous phase.
研究领域:
生物化学
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分离科学
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2022
·
IF 6.056
Lei Zhang,Ziyuan Wang,Yi Jiao,Zichen Wang,Xiuming Tang,Zhanxin Du,Zeyang Zhang,Shihai Lu,Changsheng Qiao,Jiandong Cui
Recent years, biobased polymer packaging film in food packaging has witnessed great developments. However, it is still a challenge to develop biodegradable packaging films with antimicrobial property and mechanical strength. Herein, agar and polyvinyl alcohol (PVA)/Chitosan (CS) packaging films with ε-polylysine/ZIF-L nanocomposites (ε-PL@ZIF-L) were developed for the first time. Due to the incorporation of the nanocomposite, UV/visible light barrier properties (the transmittance decreased by 10.9%) of the films were improved, and the elongation at break of the composite agar films were increased by 43.0%. In the antimicrobial assays, with 4% ε-PL@ZIF-L, the inhibition zone diameters against Bacillus subtilis were 10 times as compared with the pure PVA/CS film. Furthermore, compared with commercial PE film, agar films and PVA/CS films with 4% ε-PL@ZIF-L nanocomposites could provide long-term protection for strawberry to avoid the microbial infection and prolong the shelf life of the fruit at 25 °C. The results suggested that the agar and PVA/CS packaging films with ε-PL@ZIF-L nanocomposites could significantly behave as potent antibacterial material in food packaging and preservation systems.
研究领域:
食品科学
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材料科学
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生物技术
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产品关联
DOI
高影响因子
ANALYST
·
2024
·
IF 3.6
Yating Rui,Qunyong Tang,Liyi Chen,Juan Pu,Wangpeng Wang,Shou-Nian Ding
Baijiu, as a traditional Chinese alcoholic beverage, carries China's rich historical and cultural heritage. Consumers experience varying levels of relaxation and pleasure after consuming different Baijiu, with the biological basis of delectation influenced by serotonin and dopamine. In this study, we prepared carbon fiber electrodes modified with surface decorated gold nanoparticles to directly measure the electrochemical response signals in the serum of mice before and after gavage different types of Baijiu. It was observed that the serum signal change in mice after consuming Baijiu Sample 1 (J1) was higher than the other two types of Baijiu. Consequently, trace flavor compounds in the Baijiu samples were detected using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography mass spectrometry (LC-MS), revealing the highest content of L-lactic acid in J1. Mice were intraperitoneally injected with 200 mg/kg of L-lactic acid. The changes in dopamine and serotonin in the serum of injected mice were monitored using a biosensor, and the results were compared with results from high performance liquid chromatography-triple quadrupole mass spectrometry (HPLC-MS). The findings confirmed that L-lactic acid could indeed stimulate both neurotransmitters' secretion in mice, suggesting that the trace components in J1 may even exhibit synergistic effects. The study contributes to a deeper under-standing of the effects of Baijiu on body and provides a scientific basis for the production and consumption of Baijiu.
研究领域:
生物化学
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神经科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
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2014
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IF 3.05
Cunliu Zhou,Huiqiong Ye,Tadayuki Nishiumi,Hao Qin,Conggui Chen
The objective of this investigation was to evaluate the effects of l -histidine on the stability of porcine hemoglobin concentrates during storage. The results indicated that the addition of l -histidine increased the oxyhemoglobin content and a* values but decreased the methemoglobin content and free iron content (P < 0.05) during storage. The addition of iron cation decreased both the oxyhemoglobin content and a* values but increased both the methemoglobin content and free iron concentration (P < 0.05). CD spectra revealed that the free iron content induced the transformation of the hemoglobin secondary structure from an α-helix (close structure) to a β-pleated, β-turn, and random coil arrangement (loose structure), while l -histidine weakened this behavior of the free iron. Infrared spectra demonstrated that the l -histidine coordinated with the free iron (Fe 2 + or Fe 3 + ) to give the corresponding complex at 25 °C and pH 7.3. Therefore, l -histidine enhanced the stability of hemoglobin concentrates by coordinating with free iron.
研究领域:
生物化学
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医学工程
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DOI
高影响因子
Catalysts
·
2020
·Vol. 10
·期 9
·947
·
IF 3.52
Hongjie Hui,Yajun Bai,Tai-Ping Fan,Xiaohui Zheng,Yujie Cai
Putrescine, a biogenic amine, is a highly valued compound in medicine, industry, and agriculture. In this study, we report a whole-cell biocatalytic method inEscherichia colifor the production of putrescine, using L-arginine as the substrate. L-arginine decarboxylase and agmatine ureohydrolase were co-expressed to produce putrescine from L-arginine. Ten plasmids with different copy numbers and ordering of genes were constructed to balance the expression of the two enzymes, and the best strain was pACYCDuet-speB-speA. The optimal concentration of L-arginine was determined to be 20 mM for this strain. The optimum pH of the biotransformation was 9.5, and the optimum temperature was 45 °C; under these conditions, the yield of putrescine was 98%. This whole-cell biocatalytic method appeared to have great potential for the production of putrescine.Keywords:L-arginine;putrescine;Escherichia coli;whole-cell catalysis;co-expression
研究领域:
生物技术
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生物化学
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微生物学
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产品关联
DOI
高影响因子
Biotechnology Journal
·
2023
·Vol. 18
·期 9
·2300027
·
IF 4.7
Jian-Miao Xu,Zhou-Sheng Wu,Ke-Ji Zhao,Zhi-Jie Xi,Liu-Yu Wang,Feng Cheng,Ya-Ping Xue,Yu-Guo Zheng
Background Biocatalytic production of L-phosphinothricin (L-PPT) is currently the most promising method. In this work, we use an Escherichia coli strain coexpressing of D-amino acid oxidase and catalase ( E. coli DAAO-CAT) to oxidation biocatalytic D-PPT to PPO, then use the second E. coli strain coexpressing glutamate dehydrogenase and formate dehydrogenase ( E. coli GluDH-FDH) to reduce biocatalytic PPO to L-PPT. Main Methods and Major Results We compared the effects of different concentrations of IPTG or lactose on protein expression and enzyme activity in 5 L fermenter. The best induction conditions for E. coli DAAO-CAT were 0.05 mM IPTG, induction for 18 h at 28°C. The specific enzyme activities of DAAO and CAT were 153.20 U g −1 and 896.23 U g −1 , respectively. The optimal induction conditions for E. coli GluDH-FDH were 0.2 mM IPTG, induction for 19 h at 28°C. The specific enzyme activities of GluDH and FDH were 41.72 U g −1 and 109.70 U g −1 , respectively. The 200 mM D-PPT was biocatalyzed by E. coli DAAO-CAT for 4 h with space-time yield of 9.0 g·L −1 ·h −1 and conversion rate of over 99.0%. Then 220 mM PPO was converted to L-PPT by E. coli GluDH-FDH for 3 h with space-time yield of 14.5 g·L −1 ·h −1 and conversion rate of over 99.0%. To our knowledge, this is the most efficient biocatalytic reaction for L-PPT production. Conclusions and Implications We found that IPTG has advantages compared with lactose in the enzyme activity and biomass of E. coli DAAO-CAT and E. coli GluDH-FDH, and IPTG is more environmentally friendly. Our data implicated that IPTG can replace lactose in terms of economic feasibility and effectiveness for scaled-up industrial fermentations. Graphical and Lay Summary A two-step enzymatic method was developed for the biological preparation of L-PPT from D-PPT. In the first step, we constructed a recombinant E. coli BL21(DE3)/pCDFDuet-DAAO-CAT that utilized D-amino acid oxidase (DAAO) to catalyze the conversion of D-PPT to PPO, and catalase to degrade the hydrogen peroxide (H 2 O 2 ) produced during the reaction. Subsequently, in the second step, we constructed another recombinant E. coli BL21(DE3)/pETDuet-LcGluDH-PseFDH that employed glutamate dehydrogenase (GluDH) for the reductive amination of PPO to L-PPT, with NADH supplied by formate dehydrogenase (FDH).
研究领域:
生物化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Shuang Liang,Guanting He,Guozhe Sui,Jinlong Li,Dongxuan Guo,Ze Luo,Rongping Xu,Hong Yao,Chao Wang,Zhiyong Xing
Metal organic frameworks (MOFs) are considered as desirable precursors for the preparation of photocatalyst to improve photocatalytic hydrogen evolution efficiency due to the maintained special morphology. Herein, C-doped rod-like ZnO with porous structure has been successfully prepared by using ZIF-L as precursor via an optimized two-step calcination strategy, which is applied for photocatalytic hydrogen evolution reaction from water splitting. The results show that ZnO-350-450 exhibits the best photocatalytic activity with a hydrogen production rate of 0.35 mmol·g −1 ·h −1 , which is 1.9 times higher than that of ZnO-400 (0.184 mmol·g −1 ·h −1 ) under simulated sunlight illumination. The enhanced photocatalytic performance is attributed to the fact that the two-step calcination provides the regular porous rod-like structure (ZIF-L) and the inclusion of carbon in ZnO photocatalyst. C-doped ZnO with regular morphology offers faster charge transfer rate and higher charge separation efficiency, and the C-doped expands a wider range of light absorption. Overall, the presented work could provide a new idea for designing and preparing of photocatalysts with high efficiency for photocatalytic hydrogen production.
研究领域:
材料科学
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能源科学
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产品关联
DOI
高影响因子
CELL PROLIFERATION
·
2021
·Vol. 54
·期 5
·e13038
·
IF 6.831
Jia Ma,Jing Huang,Jinli Sun,Yanfeng Zhou,Xiaoyuan Ji,Daoxia Guo,Chang Liu,Jiyu Li,Jiye Zhang,Haiyun Song
Objectives Organic Selenium (Se) compounds such as L-Se-methylselenocysteine (L-SeMC/SeMC) have been employed as a class of anti-oxidant to protect normal tissues and organs from chemotherapy-induced systemic toxicity. However, their comprehensive effects on cancer cell proliferation and tumour progression remain elusive. Materials and Methods CCK-8 assays were conducted to determine the viabilities of cancer cells after exposure to SeMC, chemotherapeutics or combined treatment. Intracellular reactive oxygen species (ROS) levels and lipid peroxidation levels were assessed via fluorescence staining. The efficacy of free drugs or drug-loaded hydrogel against tumour growth was evaluated in a xenograft mouse model. Results Among tested cancer cells and normal cells, the A549 lung adenocarcinoma cells showed higher sensitivity to SeMC exposure. In addition, combined treatments with several types of chemotherapeutics induced synergistic lethality. SeMC promoted lipid peroxidation in A549 cells and thereby increased ROS generation. Significantly, the in vivo efficacy of combination therapy was largely potentiated by hydrogel-mediate drug delivery. Conclusions Our study reveals the selectivity of SeMC in the inhibition of cancer cell proliferation and develops an efficient strategy for local combination therapy.
研究领域:
肿瘤学
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药理学
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）