ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Wenlin Ma,Lihua Pang,Jinhua Liu,Lei Wen,Huili Ma,Yinhui Li,Zhihui Xu,Chengwu Zhang,Hai-Dong Yu
Real-time chemical and biological sensing in vitro is important for application in health and environmental monitoring. Thus, a more rapid and stable detection method is urgently needed. Herein, an immediate-stable real-time fluorescent immunosensor with a high response speed (∼100%, <1 s) and approximately zero steady-state error is constructed. The developed sensor is based on the MnO4–-triggered in situ immediate-stable fluorogenic reaction between dopamine and orcinol monohydrate to produce azamonardine (DMTM). The obtained DMTM is identified and characterized by high-resolution mass spectrometry, 1H NMR spectroscopy, 13C NMR spectroscopy, and theoretical calculations. The present sensor achieves a highly sensitive detection of dopamine (DA) with a limit of detection (LOD) of 10 nM as well as alkaline phosphates (ALP) with an LOD of 0.1 mU/mL by using orcinol monohydrate phosphate sodium salt as a substrate. As a proof of concept, ALP-triggered fluorescence ELISA using cardiac troponin I (cTnI) as a model antigen target is further constructed. The developed real-time sensor achieves the detection of cTnI with an LOD of 0.05 ng/mL. Moreover, the sensor proposed by us is successfully applied to assess the cTnI level in clinical serum specimens and yields results consistent with those obtained by the commercial ELISA method. The immediate-stable real-time fluorescence immunosensor provides a promising and powerful platform for the trace detection of biomolecules in clinical diagnosis.
研究领域:
分析化学
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生物化学
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医学诊断
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环境监测
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临床化学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 11
·1-10
·
IF 6.232
Chen Xiaolong,Wang Xianfeng,Cao Gaihua,Wu Yawen,Luo Huibo,Ji Zhong,Shen Caihong,Huo Danqun,Hou Changjun
Hemin as the organic linker ligand and Cu (II) as the metal center were applied to prepare a copper-metal-organic framework (Cu-hemin-MOF) via one-step hydrothermal method. Characterization using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray powder diffraction (XRD) demonstrate that the acquired Cu-hemin-MOF possesses the appearance of 3D ball-flower shape with the existence of C, N, O, Fe, and Cu on the surface. Further study found that this 3D ball-flower shaped Cu-hemin-MOF exhibits peroxidase-like activity, which can catalyze the peroxidase substrate of o-phenylenediamine (OPD) to generate 2,3-diaminophenazine (DAP) in the presence of H 2 O 2 . DAP has a yellow color and also emits a strong fluorescence when excited by ultraviolet light. Interestingly, Cu-hemin-MOF’s peroxidase-like activity can be strongly inhibited by glutathione (GSH). In view of this, a dual readout (fluorescence and colorimetry) was proposed to detect GSH for the first time. Under optimal conditions, the proposed method exhibits good linear relationship between the signal response (fluorescence and colorimetry) and the concentration of GSH, and low limits of detection (LOD) of 2.3 and 26.6 nM, respectively. Graphical abstract
研究领域:
材料科学
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分析化学
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生物化学
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DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2024
·
IF 5.3
Mohamed Bahri,Zina Fredj,Peiwu Qin,Mohamad Sawan
Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the ever-increasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL–1, the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL–1, surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF’s large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.
研究领域:
生物医学工程
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分析化学
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医学诊断
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癌症研究
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生物传感器
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Xinyuan Yan,Juan Chai,Luohao Yuan,Hongfeng Yin
Glutathione (GSH), as an significant intracellular antioxidant, plays a vital role in preserving the balance of cellular redox homeostasis. The accurate and sensitive detection of GSH is essential for understanding the cellular redox status and for the diagnosis and treatment of many diseases. Herein, a core–shell structure composed of glucose oxidase (GOx), platinum (Pt) nanoparticles and a zeolitic imidazolate framework (ZIF-8) shell was constructed. Pt NPs in the core–shell showed high peroxidase-like catalytic activity. The as-prepared Pt-GOx@H-ZIF-8 can be utilized for colorimetric detection of GSH, exhibiting a broad linear range from 0.5 to 90 μM, and a low limit of detection (LOD) of 0.4 μM. Pt-GOx@H-ZIF-8 showed excellent sensitivity, selectivity and stability towards glutathione detection, making it a promising candidate for intracellular antioxidants.
研究领域:
分析化学
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生物传感器
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纳米材料
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生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Jiuchuan Guo,Yudong Zhou,Jie Cheng,Fuli Chen,Jiahui Xu,Lirong Yang,Huifang Shi,Zhongfu An,Jinhong Guo,Xing Ma
Lateral flow immunoassay (LFIA), a classical point-of-care testing (POCT) technique, plays an important role in disease screening and healthcare monitoring. However, traditional LFIA is either designed for qualitative analysis or requires expensive equipment for quantification, limiting its use in household diagnosis. In this study, we proposed a new generation of LFIA for household health monitoring by using ultralong organic phosphorescence (UOP) nanomaterials as afterglow nanoprobes with a self-developed palm-size sensing device. The UOP nanoprobes exhibit a phosphorescence signal with a second-level lifetime, which completely avoids the interference from excitation light and biological background fluorescence. Therefore, an ultraminiaturized and low-cost UOP nanosensor was successfully designed by eliminating the complex optical path and filtering systems. We chose an inflammatory factor, C-reactive protein (CRP), for household POCT validation. The whole analysis was completed within 9 min. A limit of detection (LOD) of 0.54 ng/mL of CRP antigen was achieved with high stability and good specificity, which is comparable to laboratory instruments and fully satisfying the clinical diagnosis requirement.
研究领域:
生物医学工程
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分析化学
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医学诊断
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纳米技术
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即时检测设备
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Xishun Peng,Qixin chen,Xinghua Li,Li Yang,Yiheng Yuan,Cheng Zuo,Zhangyu Zhou,Zhongchen Bai
Crystal violet (CV) residues in Marine food have produced a severe health threat in human life. In this study, we proposed a semiconductor surface-enhanced Raman scattering (SERS) sensor of corrosion-resistant Polyaniline/Graphene oxide (PPy/GO) film by electrochemical growth method to detect CV residues in fresh fish tissue. A PPy/GO dispersion solution was one-step deposited on a stainless steel sheet surface by electrochemical polymerization process to form a PPy/GO composite film acting as a semiconductor SERS substrate. Since the substrate of PPy/GO film was mainly composed of GO sheet without other metals, it had a good corrosion resistance. The SERS enhancement factor and charge transfer intensity P CT of PPy/Go SERS substrate for CV molecules were up to 1.18 × 10 6 and 0.903, respectively. Furthermore, the limit of detection ( LOD ) of PPy/GO SERS substrate could reach 1.58 nM. In addition, SERS sensor of PPy/GO film could identify CV residues in fresh fish tissues, and its recovery rate was 91.8 %–107 %. This preparing method and detecting method we proposed PPy/GO SERS substrate provide a new pathway for detecting CV residues in Marine food.
研究领域:
材料科学
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分析化学
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环境科学
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食品安全
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xiaojie Song,Man-Man Lv,Qi-Yan Lv,Hui-Fang Cui,Jing Fu,Yu-Yang Huo
A novel and sensitive Helicobacter pylori assay strategy based on linear isothermal amplification reaction (LIAR) and DNAzymes catalyzed DNA sensor was developed. A 17 nt target DNA (tDNA) specific in the H. pylori genome was selected and used as primer in the LIAR. A template DNA was designed to produce an amplicon with Mg 2+ -dependent and RNA-cleaving DNAzyme (Md-Dz) activity. The amplicon (i.e. Md-Dz) was then detected with the DNA sensor. The DNA sensor was fabricated simply through immobilization of a hairpin DNA that contains both Md-Dz substrate and G-quadruplex DNAzyme (Gq-Dz), on gold electrodes. The Gq-Dz possesses peroxidase activity, catalyzing the electro-redox of the electrochemical signal probe hydroquinon. The LIAR, Md-Dz catalytic reaction, and DNA sensor fabrication and detection processes were characterized and optimized. The LIAR amplification efficiency is as high as ~5 × 10 5 fold, and the assay strategy is very sensitive, selective and repetitive. The differential pulse voltammetry (DPV) detection signals linearly correlate to the logarithmic tDNA concentration in the range of 0.1–10,000 fM, with a limit of detection (LOD) of 34 aM. The detection linear range for H. pylori genomic DNA is 2.1–67.2 pg, with an LOD of 1.3 pg . Muted DNA sequences and other pathogenic bacteria did not result in interferences. In addition, the DNA sensor is very stable in storage. More importantly, the DNA sensor and the assay strategy could be universally applicable to detect any biological sample and short DNA sequence of interests.
研究领域:
生物化学
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分析化学
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分子生物学
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生物技术
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产品关联
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2021
·Vol. 15
·期 6
·4849-4857
·
IF 2.431
Xu Yi,Kutsanedzie Felix Y. H.,Ali Shujat,Wang Pingyue,Li Chunyan,Ouyang Qin,Li Huanhuan,Chen Quansheng
Lead ion (Pb 2+ ) accumulation in food consumed by humans and animals possess health threats and effects. The development of a senor for its monitoring at its minutest accumulation in edible materials becomes an antidote to safeguarding human lives. A fabricated fluorescence-based sensor made of carboxyl modified upconversion nanoparticles (UCNPs), gold nanorods (GNRs), and cysteamine (Cys) were used as energy donor, receptor and a bridge respectively for the rapid sensing of Pb 2+ in Matcha. The UCNPs-Cys-GNRs sensor showed weak fluorescence signals due to the FRET, and was inhibited in the presence of Pb 2+ to form a complex with Cys, and the consequent recovery of fluorescence signals. Under optimized conditions, a good linear relationship was established between the relative fluorescence intensity and Pb 2+ concentrations from 1 to 100 μM with a low detection limit (LOD) of 0.5 μM. The developed sensor was applied to detect Pb 2+ in Matcha samples with recoveries within 93.99—102.16% and relative standard deviations (RSD) < 8%. Satisfactory results (p > 0.05) were obtained upon validation using a standard inductively coupled plasma mass spectrometry (ICP-MS).
研究领域:
分析化学
·
环境科学
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食品科学
·
纳米技术
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产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhihui Wu,Da-Wen Sun,Hongbin Pu,Qingyi Wei,Xuanran Lin
Mycotoxin B1 (AFB1) contamination in agricultural products pose a deadly danger to animal and human health and its rapid and reliable detection is thus very important. Herein, a ratiometric surface-enhanced Raman scattering (SERS) aptasensor for AFB1 detection was developed, in which 1,2-bis(4-pyridyl) ethylene (BPE) was used to trigger the assembly of Au nanoparticle dimers (AuNP dimers) and form intensive SERS “hot spots”, and MXenes nanosheets could load aptamer-modified AuNP dimers due to the hydrogen bonding and the chelation between the phosphate groups of aptamers and the Ti ion of MXenes. With the presence of AFB1 preferentially binding to AFB1 aptamer, AuNP dimers were separated from MXenes nanosheets, leading to a decrease in SERS intensity. Regression analysis in the range from 0.001 to 100 ng·mL −1 showed the limit of detection (LOD) being 0.6 pg·mL −1 in standard solution, indicating that the great prospects of the AuNP dimers/MXenes SERS substrate for detecting AFB1.
研究领域:
分析化学
·
纳米技术
·
食品安全
·
毒理学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yanzhu Liu,Ming Tu,Wei Lang,Qing Guo,Yongxiu Li,Qian-Yong Cao
A new amphiphilic polynorbornene-based copolymer P1 was designed and synthesized successfully by ring-opening metathesis polymerization (ROMP) of polyethylene glycol (PEG) containing monomer NB-PEG and ferreocene-anchored monomer NB-Fc . The copolymer P1 can bind with anionic uranine dye ( UD ) through multiple interactions to form no fluorescent P1 / UD nano-structure ensemble in aqueous solution. The P1 / UD ensemble can be used as indicator displacement assay (IDA) for ATP detection with a high sensitivity and selectivity. Upon the addition of ATP, the fluorescence is significant enhanced for displacement of UD from P1 / UD ensemble. The LOD value of P1 / UD ensemble toward ATP is as low as 35.9 nM. The P1 / UD ensemble exhibits an excellent photo-stability and biocompatibility, and has been successfully used for imaging ATP in living cells.
研究领域:
高分子化学
·
分析化学
·
生物医学工程
·
材料科学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Cong Fan,Nike He,Jifeng Yuan
The rapid and accurate detection of fungal pathogens is of utmost importance in the fields of healthcare, food safety, and environmental monitoring. In this study, we implemented a cascaded amplifying circuit in Saccharomyces cerevisiae to improve the G protein-coupled receptor (GPCR) mediated fungal detection . The GPCR signaling pathway was coupled with the galactose-regulated (GAL) system and a positive feedback loop was implemented to enhance the performance of yeast biosensor. We systematically compared four generations of biosensors for detecting the mating pheromone of Candida albicans , and the best biosensor exhibited the limit of detection (LOD) as low as 0.25 pM and the limit of quantification (LOQ) of 1 pM after 2 h incubation. Subsequently, we developed a betaxanthin-based colorimetric module for the easy visualization of signal outputs, and the resulting biosensors can give reliable naked-eye readouts. In summary, we demonstrated that cascaded amplifying circuits could substantially improve the engineered yeast biosensors with a better sensitivity and signal output magnitude, which will pave the way for their real-world applications in public health.
研究领域:
生物技术
·
微生物学
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生物医学工程
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环境科学
·
食品安全
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ruimin Lu,Yan Zhang,Daoping Zeng,Qihua Xu,Dan Wang,Peng Dai,Weijie Deng,Lin Kang,Xiaoxiao Ke,Luying Duan,Yanping Hong,Wuying Yang
An ultrasensitive indirect competitive chemiluminescence immunoassay (icCLEIA) based on monoclonal antibodies (mAb) for the effective detection of nitrofurantoin metabolite 1-aminohydantoin was developed under optimal conditions in our study. 1-aminohydantoin (AHD) was derivatized with m-formylphenoxyacetic acid (MCEP) to obtain a hapten MCEPAHD. The developed icCLEIA based on anti-MCEPAHD mAb showed high sensitivity to competing drugs 2-nitrophenyl derivative of AHD (2NPAHD) with a limit of detection (LOD) 0.04 ng/mL and a 50 % inhibitory concentration (IC 50 ) 0.27 ng/mL, and the icCLEIA had an ultra-high specificity for 2NPAHD, with little cross-reactivity with structural analogues other than parent drug nitrofurantoin. The mean recovery of AHD in fish samples was found to be 97.0 % to 118.5 %, with a coefficient of variation of less than 7.0 %. A comparison of the results of ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with those of icCLEIA demonstrated good linearity. These assay results indicated that the prepared mAb against MCEPAHD and the developed icCLEIA in our research can be used for simple, fast and sensitive detection of AHD residues in animal tissues.
研究领域:
分析化学
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免疫学
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兽医学
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食品安全
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）