EUROPEAN JOURNAL OF NUTRITION
·
2019
·Vol. 59
·期 6
·2709-2728
·
IF 4.449
Wang Tao,Yan Hong,Lu Yingying,Li Xin,Wang Xin,Shan Yuanyuan,Yi Yanglei,Liu Bianfang,Zhou Yuan,Lü Xin
Purpose The objective of the study was to evaluate the anti-obesity effect of Lactobacillus rhamnosus LS-8 and Lactobacillus crustorum MN047, and illustrate the potential functional mechanism about the alleviation of high fat and high fructose diet (HFFD) induced obesity and related metabolic abnormalities. Methods C57BL/6J mice were subjected to a standard or HFFD with or without supplementation of L. rhamnosus LS-8 and L. crustorum MN047 for 10 weeks. Obesity related metabolic indices including glucose tolerance, insulin resistance, serum lipid, liver function, hormones and inflammatory cytokines were assessed by standard protocols. For the monitoring of inflammatory response and lipid metabolism, transcriptional levels were profiled in liver and/or adipose tissues. Furthermore, gut microbiota composition analyses in the fecal samples were performed using 16S rRNA gene sequencing, and gut microbial metabolites, including lipopolysaccharide (LPS) and short-chain fatty acids (SCFAs), were also tested for the assessment of the relationship between gut microbiota variation and inflammatory response. Results Administration with L. rhamnosus LS-8 and L. crustorum MN047 significantly mitigated body weight gain and insulin resistance, and inflammatory response (TNF-α, IL-1β and IL-6 levels in serum and corresponding mRNA levels in adipose tissues) was significantly inhibited in these two strains-treated mice. Moreover, L. rhamnosus LS-8 and L. crustorum MN047 could partially normalized mRNA expression levels involved in lipid metabolism including Pparγ , Srebp - 1c , CD36 , Fabp2 and FAS . In addition, these two strains manipulated gut microbiota by decreasing the abundance of Bacteroides and Desulfovibrio and increasing that of Lactobacillus and Bifidobacterium , which in turn raised the levels of feces SCFAs and lowered the levels of circulating LPS. Conclusion These results indicated that L. rhamnosus LS-8 and L. crustorum MN047 supplementation possessed the anti-obesity effect on the HFFD fed mice by alleviating inflammatory response and regulating gut microbiota, which further suggested that these two probiotics can be considered as an alternative dietary supplement in combination with the preventive and therapeutic strategies against obesity and related complications. Graphic abstract
研究领域:
微生物学
·
营养学
·
分子生物学
·
胃肠病学
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2022
·
IF 6.064
Liu Chen,Xue Wen-jiao,Ding Hao,An Chao,Ma Sai-jian,Liu Yao
The objective of this study was to assess in vitro probiotic potential of Lactobacillus strains derived from artisanal fermented vegetables in Shaanxi, China. In total, 74 acid-producing Gram-positive strains with rod-shaped under the microscope were isolated from 16 samples of spontaneously fermented vegetables. Out of 74 strains, 26 showed high survival rate under low pH and high bile salts conditions and were subjected to molecular identification by 16S rRNA gene sequencing analysis. The results showed that 15 isolates belonged to Lactobacillus plantarum, 9 isolates belonged to Lactobacillus brevis, and the 2 remaining strains belonged to Weissella viridescens. The 24 Lactobacillus strains were investigated for their survival rate to transit simulated gastrointestinal tract, cell surface hydrophobicity, auto-aggregation, co-aggregation with pathogen, adhesion to Caco-2, antimicrobial activity, antibiotics susceptibility, radical scavenging ability, α-glucosidase inhibition, and the cholesterol assimilation. The results showed that the probiotic characteristics were strain-dependent, and several strains exhibited great probiotic potential with specific health benefits, which indicated that they might be excellent candidates for production of functional foods. Interestingly, it was first found that L. plantarum generally had higher antibacterial activities, α-glucosidase inhibition ability, and antibiotics susceptibility compared to L. brevis in this study. The results indicated that Lactobacillus strains isolated from fermented vegetables in Shaanxi, China, could be exploited as a promising novel probiotic source.
研究领域:
微生物学
·
食品科学
·
营养学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2016
·
IF 3.858
Wei Li,Ya Zhu,Feng Ye,Bin Li,Xiaogang Luo,Shilin Liu
Cellulose-based microgels, prepared by the sol-gel transition method, were evaluated to extent the loading capacity and make more controlled release of probiotics . Granules of calcium carbonate as porogenic agents made the cellulose microgels with different porosity, and the S BET of modified cellulose microgels was increased to 197 m 2 /g. The microgels were constituted by the interconnected network of the cellulose nanofibrils, which was house-like. The house-liked microgels had the ability for the accommodation of Lactobacillus plantarum ( L. plantarum ) as high as 10 10 cfu/g, and the released viable cells was up to 3.10 × 10 10 cfu/g. Furthermore, the Ca-alginate shell protection of the cellulose houses kept the loaded L. plantarum cells from liberating into stomach but released the cells at intestinal tracts due to the effect of pH-responsibility. From the cross-sectional fluorescence image, L. plantarum cells mainly focused on the cellulose microgels houses instead of Ca-alginate shell. It indicated that the surface modification of the cellulose microgels houses by using alginate based hydrogel exhibited more controlled release of L. plantarum cells, which could be increased to 5.80 × 10 8 cfu/mL at 360 min. Prolonged duration in simulated intestine fluid contributed to making sure that cells reached the desired region of intestine tracts. The designed composite system advances in affording better shelter for probiotic residence or other bioactive nutrients.
研究领域:
食品科学
·
微生物学
·
材料科学
✓ 已验证
DOI
高影响因子
TOXICOLOGY LETTERS
·
2021
·
IF 4.374
Jie Liu,Yi Cheng,Yuanjin Zhang,Shengbo Huang,Zongjun Liu,Xin Wang
Verapamil, a calcium channel blocker, has been approved as the first-line drug for treatment of angina pectoris, hypertension and supraventricular tachycardia. Lactobacillus rhamnosus , one of the normal strains in human intestinal tract, is very popular in the probiotic market for conferring a health benefit on the host. This report investigated the potential of gut microbiota-drug interactions between lactobacillus rhamnosus and verapamil via using wild type (WT) and Cyp3a1/2 knockout (KO) rats. In WT rats, administration of Lactobacillus rhamnosus for 14 days decreased systemic exposure of verapamil and increased its metabolite norverapamil in vivo , and resulted in gut microbiota-drug interactions. In Cyp3a1/2 KO rats, however, this interaction disappeared. Further studies found that Lactobacillus rhamnosus induced CYP3A activity and expression, and changed the composition of gut microbiota, thus changing the pharmacokinetics of verapamil. These results demonstrated the interaction between lactobacillus rhamnosus and verapamil, and indicated that the effect of gut microbiota on metabolic enzymes cannot be ignored.
研究领域:
药理学
·
微生物学
·
胃肠病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Innovative Food Science & Emerging Technologies
·
2023
·
IF 6.6
Chao Ma,Xiaoqian Zhang,Kaiqiang Wang,Jingya Jiang,Jianhua Zeng,Lanwei Zhang,Pimin Gong
Employing 0.64 mg/mL of rifampin (RFP) for dormancy induction of Lactobacillus bulgaricus sp 1.1, a 2.5-fold survival rate was achieved after spray drying, while the dried bacteria displayed delayed acidifications . Under the same condition for dormancy treatment, division analysis indicated that high proportions of cells would enter into a dormant state with around 90% of non-proliferating bacteria in the original population. Cells shrank with median diameters decreasing from 3.23 to 2.98 μm. The Raman spectrum revealed a lower ATP level, varied protein expression profiles and conformations and saccharides accumulation in bacteria. Protein aggregates formed in dormancy-induced cells may contribute to increased heat resistance which showed a 2 °C rise in the denaturing temperature in the 0.64 mg/mL group. Therefore, dormancy treatments may be developed as a protective strategy for spray-dried L . bulgaricus . Industrial relevance Enhancing the drying tolerance of bacteria is one of the crucial strategies to adapt the industrial production of spray-dried LAB . Our study proved that the non-sporeforming dormancy pre-treatment can promote the spray-dried viability of L . bulgaricus by triggering suppressed metabolism activity and protein aggregation. The non-sporeforming dormant state has potential to be exploited in industrial production of spray-dried LAB with a high viable cell density to reduce the cost of probiotics and related products.
研究领域:
微生物学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Cong Xu,Jiage Ma,Zhijing Liu,Wan Wang,Xin Liu,Shanshan Qian,Lijun Chen,Liya Gu,Chuanqiang Sun,Juncai Hou,Zhanmei Jiang
In this study, shell-core fibers were successfully prepared by using Eudragit S100 (ES100) and poly(vinyl alcohol) (PVA)/pectin (PEC) through coaxial electrospinning technology. The electrospun fiber was characterized by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray diffraction (XRD). Thermo-gravimetric analysis (TGA) showed that the coaxial electrospun fiber encapsulated with Lactobacillus rhamnosus 1.0320 ( L. rhamnosus) had higher thermal stability than the electrospun fiber prepared by uniaxial electrospinning. L. rhamnosus encapsulated by coaxial electrospun fiber maintained 90.07% and 91.96% survivability in simulated gastric and intestinal fluids. After continuous simulated gastrointestinal fluid treatment, the survival rate of L. rhamnosus encapsulated by coaxial electrospun fiber was 81.40%. The results indicate that shell-core fiber-encapsulated probiotics can improve the tolerance of probiotics to the harsh environment of gastrointestinal tract . The fiber prepared in this study can be applied to the preparation of functional fermented food such as probiotic yogurt fermentation in the future.
研究领域:
材料科学
·
生物技术
·
食品科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2023
·
IF 5.2
Xiaoying Tian,Xi Liang,Hong He,Qingyu Cui,Qiqi Liu,Rongbo Fan,Tongjie Liu,Huaxi Yi,Pimin Gong,Qingzhu Wang,Lanwei Zhang
Scope Food allergy has become a world recognized public health problem due to its versatility and lack of efficacious methods for its treatment. Probiotics supplement is a potential way to prevent food allergy. Methods and results In this study, potential strains are screen out by peripheral blood mononuclear cells (PBMCs), and their abilities of alleviating food allergy are examined using a mouse model induced by ovalbumin (OVA). The results show that six strains increase ratio of interferon-γ (IFN-γ)/interleukin (IL)-4 secreted by PBMCs with good abilities in intestinal adhesion and gastrointestinal tolerance. Oral administration of Bifidobacterium animalis KV9 (KV9) and Lactobacillus vaginalis FN3 (FN3) attenuates allergic responses in allergy mice, including allergic symptoms, mast cells aggregation and activity, serum OVA-special-immunoglobulins E (OVA-sIgE) production. KV9 and FN3 upregulate the production of IFN-γ/IL-4 in splenocytes, increase the genes and proteins expression of toll-like receptor 4 (TLR4), myeloid differentiation primary response 88 (Myd88) and interferon regulatory factor (IRF)-1 in allergic mice spleen, and decrease the IRF-4. Conclusion The study demonstrates that KV9 and FN3 possess anti-allergic activities via activation of TLR4 pathway and modulating the expression of IRF-1 and IRF-4 which leads to T helper type 1 (Th1)/T helper type 2 (Th2) cell immunology balance.
研究领域:
免疫学
·
微生物学
·
过敏
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Microbiome
·
2022
·Vol. 10
·期 1
·1-15
·
IF 16.837
Meng Yujie,Li Shuang,Zhang Chong,Zheng Hao
Background Symbiotic gut microbes have a rich genomic and metabolic pool and are closely related to hosts’ health. Traditional sequencing profiling masks the genomic and phenotypic diversity among strains from the same species. Innovative droplet-based microfluidic cultivation may help to elucidate the inter-strain interactions. A limited number of bacterial phylotypes colonize the honeybee gut, while individual strains possess unique genomic potential and critical capabilities, which provides a particularly good model for strain-level analyses.Results Here, we construct a droplet-based microfluidic platform and generated ~ 6 × 108 droplets encapsulated with individual bacterial cells from the honeybee gut and cultivate in different media. Shotgun metagenomic analysis reveals significant changes in community structure after droplet-based cultivation, with certain species showing higher strain-level diversity than in gut samples. We obtain metagenome-assembled genomes, and comparative analysis reveal a potential novel cluster from Bifidobacterium in the honeybee. Interestingly, Lactobacillus panisapium strains obtained via droplet cultivation from Apis mellifera contain a unique set of genes encoding l-arabinofuranosidase, which is likely important for the survival of bacteria in competitive environments.Conclusion sBy encapsulating single bacteria cells inside microfluidic droplets, we exclude potential interspecific competition for the enrichment of rare strains by shotgun sequencing at high resolution. The comparative genomic analysis reveals underlying mechanisms for host-specific adaptations, providing intriguing insights into microbe-microbe interactions. The current approach may facilitate the hunting for elusive bacteria and paves the way for large-scale studies of more complex animal microbial communities.
Video Abstract
研究领域:
微生物学
·
基因组学
·
宏基因组学
·
微流控技术
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2021
·Vol. 45
·期 9
·e15291
·
IF 2.19
Jingbo Zhang,Chaofan Ji,Jing Han,Yunsong Zhao,Xinping Lin,Huipeng Liang,Sufang Zhang
Toxic biogenic amines (BAs), especially histamine, in food is a major safety issue. Two autochthonous Lactobacillus plantarum strains were inoculated as starters to reduce BAs in Yucha, a kind of spontaneously fermented fish in China. Taxonomic analysis demonstrated the proportion of Lactobacillus genus in inoculated Yucha was higher than that in the spontaneously fermented Yucha, suggesting the effective colonization abilities of the starters. Higher abundance of Lactobacillus genus resulted in less BAs contents in the inoculated Yucha as compared to that in the spontaneously ones throughout the fermentation stage. At the end of fermentation, L. plantarum strains reduced total content of the six BAs by 67.5%–97.3%; specifically, they reduced the concentration of histamine by 85.4%–96.4%. The BAs-degradation ability in the model systems indicated that the two L. plantarum strains could not degrade BAs directly. Instead, starters reduced BAs accumulation by inhibition of the growth or biological activities of BAs-producing bacterial genera. Practical applications The presence of biogenic amines (BAs) in fish products poses a health risk for consumers. This work provided two promising starters to reduce BAs accumulation in Yucha and a foundation for better understanding the BAs metabolic features. These findings could contribute to the safe production of Yucha and other similar fermented fish products.
研究领域:
食品科学
·
微生物学
·
生物技术
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2026
·
IF 6.2
Siru Zhao,Jiashuo Chen,Yueying Zhao,Shuang Zhang
Metal-phenolic networks (MPNs) exhibit rapid film formation and surface modification potential, but their material selection and mechanisms are insufficiently explored. This study used gallic acid (GA), epigallocatechin (EGC), and epigallocatechin gallate (EGCG) as polyphenol precursors to construct MPNs coatings on Lactobacillus plantarum ( L. plantarum ) via coordination with ferric (Fe 3+ ) or calcium (Ca 2+ ) ions. Systematic analysis showed that polyphenol structure and metal ion (Fe 3+ > Ca 2+ ) significantly affect coating performance, with EGCG-Fe 3+ coatings achieving the greatest thickness (1584 nm), strongest metal-phenolic coordination, and highest stability. This coating also demonstrated remarkable functional enhancements: superior antioxidant capacity (75.9% DPPH and 98.96% ABTS scavenging rates), high antibacterial rate (78.7%), and excellent gastrointestinal tolerance, maintaining viable cell counts above 10 7 CFU/mL throughout digestion stages. These findings clarify MPNs formation mechanisms, laying a theoretical basis for optimizing polyphenol-metal coordination in probiotic encapsulation systems to enhance functionality and expand applications in probiotic food development.
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-20
·
IF 15.7
Zhang Jiayu,Yang Jinneng,Luo Jinyan,Wu Weili,Luo Haidan,Wei Wenxia,Lyu Haimei,Wang Yuzhi,Yi Hairong,Zhang Yijing,Fan Zongmin,Lyu Haiwen,Kanakaveti Vishnu Priya,Qin Baifu,Yuan Ping,Yang Runxiang,Zhang Haipeng,Zuo Tao,Felsher Dean W.,Lee Mong-Hong,Li Kai
Oncolytic viruses (OVs) hold promise for cancer treatment. However, the antitumor efficacy is limited. Microbiota plays a pivotal role in cancer treatment and its impact on oncolytic virotherapy is unknown. Here, we show that VSVΔ51 has higher antitumor efficacy for hepatocellular carcinoma in the absence of microbiota in female mouse models. VSVΔ51 infection causes microbiota dysbiosis, increasing most of the gut bacteria abundance, while decreasing the commensal Lactobacillus . VSVΔ51 reduced intestinal expression of SLC20A1 that binds to Lactobacillus acidophilus ( L. acidophilus ) CdpA cell wall protein through IL6-JAK-STAT3 signaling, thereby attenuating attachment and colonization of L. acidophilus . L. acidophilus supplementation confers sensitivity to VSVΔ51 through restoring gut barrier integrity and microbiota homeostasis destroyed by VSVΔ51. In this work, we show that targeting microbiota homostasis holds substantial potential in improving therapeutic outcomes of oncolytic virotherapy.
研究领域:
肿瘤学
·
微生物学
·
病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Xiumin Zhang,Ronggang Liu,Yannan Chen,Huihui Wang,Wentao Su,Yukun Song,Mingqian Tan
Oral nutritional interventions for nonalcoholic steatohepatitis (NASH) have garnered significant interest due to their potential benefits. Astaxanthin (AXT) has the potential to enhance liver function and act as an effective antioxidant for NASH intervention, but its application is limited by its stability and bioavailability. This study aims to develop dual-targeted AXT nanoparticles (AXT@TWG) for precise liver-targeted delivery by ″hitchhiking″ on Lactobacillus rhamnosus bacterial ghosts (LBGs) to effectively intervene in NASH. In vitro experiments demonstrated that AXT@TWG nanoparticles significantly reduced LPS-induced reactive oxygen species production and apoptosis while effectively alleviating lipid accumulation. In vivo experiments demonstrated that LBGs significantly enhanced the intestinal accumulation efficiency of AXT@TWG. Pharmacokinetic evaluations revealed that the efficiency of AXT@TWG@LBGs entering the bloodstream was approximately 2.7 times higher than that of AXT@TWG nanoparticles and their accumulation in the liver was about 1.3 times greater. AXT@TWG@LBGs effectively alleviated NASH by reducing triglycerides, free fatty acids, and malondialdehyde levels by 23.07, 65.32, and 21.42%, respectively, compared to the model group, thereby mitigating lipid accumulation and enhancing antioxidant capacity. Additionally, AXT@TWG@LBGs effectively reduced insulin resistance, lowered inflammatory cytokine levels, and corrected disturbances in lipid metabolism. Therefore, this study provides a potentially effective strategy for the treatment of NASH.
研究领域:
生物医学工程
·
药理学
·
肝脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）