ENGINEERING IN LIFE SCIENCES
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2018
·Vol. 19
·期 1
·57-65
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IF 2.385
Jianming Zhang,Huaxi Yi,Pimin Gong,Kai Lin,Shiwei Chen,Xue Han,Lanwei Zhang
Kieselguhr, bentonite, and montmorillonite were investigated as potential carriers of plantaricin Q7. Highest level of adsorption of plantaricin Q7 was obtained with montmorillonite. Meanwhile, visible inhibition zones were observed against Listeria monocytogenes for montmorillonite adsorbed with plantaricin Q7. Adsorption kinetics showed that the adsorption behaviour followed the pseudo-first-order and Weber's intra-particle diffusion models, suggesting two steps had taken place during the adsorption process. X-ray diffraction assays revealed that plantaricin Q7 was intercalated into the interlayer space of montmorillonites. Electrostatic, hydrogen bonding and hydrophobic interactions proved to play important roles in the mechanisms of interaction between montmorillonite and plantaricin Q7, as shown by infrared spectroscopy analysis. In addition, plantaricin Q7 production was inhibited by feedback regulation with its high concentrations. In order to remove product feedback inhibition in plantaricin Q7 production, a strategy was implemented for its adsorption onto montmorillonite during fermentation. The final plantaricin Q7 output reached 3713.40 IU/mL during fermentation using montmorillonite to adsorb plantaricin Q7, 41.61% higher than that of non- montmorillonite. These results indicate that montmorillonites are suitable carriers for plantaricin Q7 adsorption, and the adsorption of plantaricin Q7 onto montmorillonite during fermentation could be a good method to increase final plantaricin Q7 production.
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JOURNAL OF DAIRY SCIENCE
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2019
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IF 3.082
Zhengqi Liu,Lingyi Dong,Kaiying Jia,Hui Zhan,Zhihong Zhang,Nagendra P. Shah,Xueying Tao,Hua Wei
ABSTRACT Exopolysaccharide (EPS) of Lactobacillus plantarum WLPL04 and its sulfated EPS were systematically investigated for their antioxidant activities and effects on protecting the oxidative damage of Caco-2 cells from H 2 O 2 . Exopolysaccharide was successfully sulfonated from purified EPS as confirmed by Fourier-transform infrared spectroscopy, and the degree of sulfonation was 0.30. Both EPS and sulfated EPS showed antioxidant activities in vitro determined by 1,1-diphenyl-2-picrylhydrazyl, superoxide, and hydroxyl radical scavenging tests, and those activities of sulfated EPS were significantly enhanced at 1,000 μg/mL. Cell viabilities of Caco-2 in the range of 1 to 100 μg/mL of EPS and sulfated EPS showed no significant difference. In H 2 O 2 -damaged Caco-2 cells models, EPS and sulfated EPS significantly inhibited the enhancement of reactive oxygen species and malondialdehyde levels, and sulfated EPS enhanced the effects by 40.86% and 61.11% when compared with the purified EPS at the same concentration of 100 μg/mL, respectively. For the activities of antioxidant-related enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and expression of genes ( SOD2 , GPX2 , MT1M ) on Caco-2 cells, strong protection abilities against the oxidative stress were displayed from both EPS and sulfated EPS, and sulfated EPS exhibited significant enhancement as compared with either EPS or control groups. In summary, sulfonation is an effective strategy for improving the antioxidant activities of EPS from L. plantarum WLPL04 in vitro and on Caco-2 cells.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Zhen Wang,Sijie Zhang,Huiping Wang,Jihong Huang,Luyang Wang
Hyaluronic acid (HA), as a multifunctional hydrophilic polysaccharide , is potentially beneficial in improving the thermal stability of fermented modified starches, but relevant insights at the molecular level are lacking. The aim of this study was to investigate the effect of different levels (0 %, 3 %, 6 %, 9 %, 12 % and 15 %) of HA on the structural, thermal and pasting properties of wheat starch co-fermented with Saccharomyces cerevisiae and Lactobacillus plantarum . We found that the addition of HA increased the median particle size of fermented starch granules from 16.387 to 17.070 μm. Meanwhile, the crystallinity of fermented starch was negatively correlated with the HA content, decreasing from 14.70 % to 12.80 % ( p < 0.05). Fourier transform infrared spectroscopy results confirmed that HA interacted with starch granules and water molecules mainly through hydrogen bonding. Thermal analyses showed that the thermal peak of the composite correlated with the HA concentration, reaching a maximum of 73.17 °C at 12 % HA. In addition, HA increases the pasting temperature, reduces the peak, breakdown and setback viscosities of starch. This study demonstrates the role of HA in improving the thermal stability of fermented starch, providing new insights for traditional fermented food research and the application of HA in food processing.
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Biology-Basel
·
2020
·Vol. 9
·期 7
·171
·
IF 3.796
Jianming Zhang,Yushan Bu,Chengcheng Zhang,Huaxi Yi,Daqun Liu,Jingkai Jiao
At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/106AU) than in the MRS broth (46.82 RMB/106AU).Keywords:bacteriocin Lac-B23;low-cost;high-efficiency;culture medium
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Qinchao Ding,Feiwei Cao,Shanglei Lai,Hui Zhuge,Kaixin Chang,Teresa G. Valencak,Jianxin Liu,Songtao Li,Daxi Ren
This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10 9 cfu/day) were administered by oral gavage, respectively. Our data showed that L. plantarum ZY08, but not E680, intervention significantly mitigated alcohol-related hepatic steatosis, liver injury, intestinal barrier, and it alleviated plasma endotoxin (LPS) levels, and affected hepatic genes relating to lipid metabolism. Furthermore, Lactobacillus plantarum ZY08 effectively restored intestinal flora homeostasis via recovering flora abundance, including Blautia, Oscillibacter , Lachnoclostridium and Intestimonas , and consequently elevated intestinal short-chain fatty acid (SCFA) content. More importantly, removing intestinal microorganisms through ABX gavage markedly abolished the beneficial aspects of Lactobacillus plantarum ZY08, indicating that the regulative role of Lactobacillus plantarum ZY08 contributed to its protective role against ALD. Overall, Lactobacillus plantarum ZY08 is a potential candidate for mitigating alcohol-induced hepatic steatosis and liver injury.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Pengrui Wu,Yefan Niu,Zejie Chen,Chunling Nie,Yijun Liu,Zhonghui Shen,Zhihong Song,Xindi Wei,YanBo Wang,Hong Zeng,JianGuo Wang
In this study, pullulan-in-hydroxypropyl methylcellulose (PUL/HPMC) water-in-water Pickering emulsions stabilized by cellulose nanocrystals (CNCs) were prepared for the encapsulation and cultivation of L. plantarum . Results demonstrated that CNCs were mainly adsorbed at the PUL-HPMC interface, thereby effectively inhibiting the aggregation and coalescence of emulsion droplets. L. plantarum was encapsulated in emulsions and primarily distributed in the PUL phase. It was found that the optimal encapsulation concentration of L. plantarum was 9.67 log CFU/g, and the optimal addition of MRS broth was 1.0 wt%. Finally, the viable counts of L. plantarum increased from 4.44 to 7.42 log CFU/g during 30 h of cultivation. Furthermore, an initial pH 5.5 afforded the optimal cultivation performance for L. plantarum , with viable counts increasing from 4.00 to 7.06 log CFU/g during 24 h of cultivation. This study provides a novel microculture platform for efficient L. plantarum cultivation and fully aqueous emulsion systems with food-grade application potential for probiotics-based functional foods. Download: Download high-res image (143KB) Download: Download full-size image
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Frontiers in Nutrition
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2022
·
IF 6.59
Feng Nianjie,Tang Fei,Hu Chuanqin,Cheng Lei,Lv Zhejuan,Shen Yang,Li Wei,Xiao Gengsheng,Dong Hao,Wu Qian
Lactic acid bacteria (LAB) have already been used as fermentation strains to enhance the antioxidant capacity of polyphenols. Antioxidant capacity is one of the most important factors to inhibit advanced glycation end product (AGE) formation and could LAB increase the inhibitory capacity of procyanidins on AGEs formation? It was surprising that opposite results were obtained both in simulated food processing and gastrointestinal digestion systems. After incubation with Lactobacillus plantarum (L. plantarum), litchi pericarp oligomeric procyanidins (LPOPCs) were bioconverted to several phenolic acids, which increased the antioxidant activity as expected. However, antiglycation ability and trapping carbonyl compounds capacity both weakened and it might be the primary reason for decreasing the inhibitory effect on AGE formation. Furthermore, it was found that LPOPCs incubated with L. plantarum inhibited the activity of digestive enzymes and thus decreased the digestibility of glycated protein. Our study systematically proposed for the first time that procyanidins bioconversion is an effective means to improve the antioxidant activity but has no remarkable promoting effect on AGEs inhibition.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yanli Du,Soon-Mi Shim,Lei Wang,Xin Gao,Xiaoting Fu
Fermentation can promote various properties of food. This study investigated the impact of fermentation by Monascus purpureus combined with Lactobacillus plantarum and Saccharomyces cerevisiae on the physicochemical and flavor characteristics of Pyropia yezoensis . Results indicate that the protein and free amino acid content increased threefold. A total of 62 volatile flavor compounds were identified by GC-IMS. Alcohols, ketones and esters were enhanced with 2- to 6-fold, while aldehydes were reduced by 76.56 %, contributing to the enhancement of pleasant fragrances and the reduction of fishy and seawater odors. As for the non-volatile properties, the content of organic acids and free amino acids increased from 2.18 mg/g and 0.19 mg/g to 46.03 mg/g and 4.93 mg/g, respectively, as assessed by HPLC and amino acid analyzer. The metabolic pathways of non-volatile substances were speculated upon. This work provides a basic theory and a practical reference for fermentation food processing of P. yezoensis .
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Food Bioscience
·
2024
·
IF 4.8
Cui Cao,Yiping Gao,Feilong Sun,Chunqing Ai,Shuang Song
Arabinogalactan from Lycium barbarum (LBP-3) is the major biological activity component and contributes to maintaining intestinal immune homeostasis by targeting gut microbiota and their metabolites. However, it is still a big challenge to know the specific ecological microbe-metabolite interaction regulated by LBP-3 due to the host complex diet webs and lots of unidentified intestinal bacteria. Therefore, the present study constructed a defined microbial consortium, composing of symbiotic Bacteroides caccae, Phocaeicola vulgatus , B. thetaiotaomicron , B. uniformis , B. ovatus , Lactobacillus plantarum and Escherichia coli in vitro , to investigate the impact of LBP-3 on the interactions of species and their metabolites. The utilization of LBP-3 by the defined microbial consortium of 7 intestinal species was 22.5%. Full-length 16S rRNA analysis demonstrated that LBP-3 significantly modulated the composition of synthetic bacterial community, especially inhibiting the level of opportunistic pathogenic E. coli and improving the relative abundance of probiotic L. plantarum and all Bacteroidetes species except B. ovatus . LBP-3 remarkedly influenced 27 differential metabolites, mainly belong to amino acids, peptides and analogues (37.04%) and carboxylic acid and derivatives (7.41%). Moreover, targeted metabolomics revealed that the levels of acetate, propionate, tryptophan and 5-methylthioadenosine were improved by LBP-3. In addition, LBP-3 modulated the pathways of tryptophan metabolism, cysteine and methionine metabolism, and biosynthesis of siderophore group nonribosomal peptides. Notably, some amino acids (e.g., proline and glutamate) and their related metabolic pathways (e.g., arginine and proline metabolism) altered by LBP-3 were significantly correlated with the levels of species, such as B. uniformis , P. vulgatus, and B. thetaiotaomicron .
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Cong Xu,Jiahui Guo,Baoyue Chang,Qingyun Wang,Yiming Zhang,Xianhui Chen,Wanyi Zhu,Jiage Ma,Shanshan Qian,Zhanmei Jiang,Juncai Hou
This study was conducted to develop a W/O/W emulsion encapsulated Lactobacillus plantarum 23 – 1 ( LP23 – 1 ) to significantly enhance the survival rate of LP23-1 under simulated digestion and storage conditions. The zein particles and pectin formed a complex through electrostatic interaction and hydrogen bonding. When the proportion of zein particles to pectin was 1:1, the emulsifying stability index (ESI) was 304.17 %. Additionally, when the proportion of the internal aqueous phase to the oil phase was 1:9, the polyglycerol polyricinoleate (PGPR) concentration was 5 %, the proportion of primary emulsion to the external aqueous phase was 5:5, the zein particles concentration was 4 %, and the proportion of zein particles to pectin was 1:1, the encapsulation rate was the highest at 96.27 %. Cryo-scanning electron microscopy and fluorescence microscopy confirmed the morphology of W/O/W emulsion and successful encapsulation of LP23–1 . Furthermore, compared with free LP23–1 , the W/O/W emulsion encapsulation significantly improved the survival rate of LP23–1 to 73.36 % after simulated gastrointestinal digestion and maintained a high survival rate of 78.42 % during the 35-day storage. The W/O/W emulsion was found to effectively improve the survival rate of LP23–1 during simulated digestion and storage, which has implications for the development of probiotic functional foods with elevated survival rates.
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Food Chemistry-X
·
2024
·
IF 6.5
Mengya Zhao,Hong Ren,Zhuomin Yan,Jing Ma,Xiaoping Feng,Di Liu,Fangyu Long
Ochratoxin A (OTA) contamination in grape juice has attracted widespread concern as OTA can lead to kidney disease and cause adverse neurological effects. An effective method to remove OTA is to make use of highly adsorbent materials that are able to remove the toxic contaminant. Recently, inactivated Lactobacillus plantarum- based biosorbents have shown to be an efficient, cost-effective and environmentally friendly bioremediation method in removing toxic pollutants such as OTA. We used five chemical thiol-modification methods to improve the adsorption efficiency of OTA in grape juice. The esterification of Lactobacillus plantarum (L-Es) significantly increased the sulfhydryl contents (-SH) by 251.33 μmol/g and >90% of OTA was removed. However, the inactivated microbial adsorbent was difficult to separate after adsorption and therefore, the prepared L-Es were embedded into the cellulose nanocrystals (L-Es@CNCs). Moreover, L-Es@CNCs significantly increased the adsorption rate of OTA in grape juice samples by 88.28% with negligible effects on juice quality due to the properties of easy re-use and excellent biodegradability. This showcases its potential application for OTA removal in the grape juice industry.
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FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Xin Luo,Siqi Fan,Zhuosui He,Fangfang Ni,Chengzhi Liu,Min Huang,Lei Cai,Gerui Ren,Xuan Zhu,Qunfang Lei,Wenjun Fang,Hujun Xie
Probiotics are sensitive to external conditions, resulting in low survival rates after being ingested or during food production, transportation and storage. In order to improve the survival rate of Lactobacillus plantarum (LP) during gastrointestinal digestion, storage, and freeze-drying, alginate-whey protein isolate (ALG-WPI) and alginate-pectin-whey protein isolate (ALG-PEC-WPI) composites were employed to encapsulate LP. The encapsulation efficiency of ALG-WPI-LP and ALG-PEC-WPI-LP beads both reached more than 99 % . Scanning electron microscopy (SEM) indicated that dense and rough aggregates were formed on the surface of both composites, and attached LP cells could be observed inside the beads. The ALG-WPI and ALG-PEC-WPI composites can protect the viability of LP in simulated gastric fluid (SGF) and release the probiotics in simulated intestinal fluid (SIF). The storage stability of LP at 4 °C was improved by about 15 % in comparison with bare LP and the survival rates of LP in ALG-WPI-LP and ALG-PEC-WPI-LP powders after freeze-drying were increased by 65.37 % and 72.06 %, respectively. The formation mechanism of ALG-WPI and ALG-PEC-WPI composites was further explored by fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The ALG-WPI and ALG-PEC-WPI composites have great potential to protect and deliver probiotics in food systems.
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