ENVIRONMENTAL RESEARCH
·
2026
·
IF 8.2
Yafei Duan,Yangguang Gu,Zhe Zhang,Meng Xiao,Ruijie Zhu,Jitao Li
Polychlorinated biphenyls (PCB) are typical persistent organic pollutants widely present in the environment, which commonly contaminate coastal aquaculture waters. Clarifying their toxic effects on edible shrimp is of great significance for healthy aquaculture development and food safety. This study conducted a 14-day subchronic exposure of Litopenaeus vannamei to 1 ng/L and 10 ng/L PCB1254 via waterborne exposure simulating environmental pollution levels, and systematically explored the toxicological effects of PCB at different biological levels using integrated multi-omics techniques. The results showed that PCB exposure reduced the survival and ammonia stress tolerance of the shrimp, and induced histological alterations and oxidative stress in the gills, manifested as the accumulation of lipid peroxidation products and the disruption of redox homeostasis. It affected ferroptosis homeostasis in the gills by inducing iron accumulation and altering the expression of gene markers, disturbed the expression of antimicrobial peptide genes (e.g., ALF , Pen3 ), and increased the expression of inflammation-related genes (e.g., JNK , TNFα ). Furthermore, the metabolic patterns in the gills were also disrupted, especially the pathways of amino acid metabolism and ABC transporters, as well as arachidonic acid and tryptophan metabolites. In addition, intestinal histological morphology was damaged, and microbial communities were disturbed, manifested as reduced diversity, altered abundance of functional bacteria (e.g., Vibrio , Pseudoalteromonas , Bacteroides and Lactobacillus ), and disrupted metabolic functions (e.g., carbohydrate absorption, β -lactam resistance). Finally, the nutritional value of the muscle was impaired by reducing the contents of crude protein, total amino acids and delicious amino acids. In conclusion, this study revealed that exposure to environmentally relevant concentrations of PCB-1254 induced toxic effects on shrimp by triggering histological alterations, oxidative stress, ferroptosis, metabolic reprogramming and intestinal microbiota disorder, and altered their muscle nutritional quality.
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高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Du Qi,Fang Chuangdong,Qi He,Benjakul Soottawat,Aubourg Santiago P.,Zhang Bin
Phosphates and trehalose are widely accepted additives in animal muscle products. In this study, the effects of pre-soaking with sodium tripolyphosphate (STPP) and trehalose under vacuum permeation (VP) conditions on the physicochemical properties of shrimp muscle were evaluated over 120 d of frozen storage. The results indicate the STPP/trehalose-VP treatments significantly reduced the thawing loss and prevented changes in the texture, myofibrillar protein (MP) content, and Ca2+-ATPase activity of shrimp muscle during frozen storage compared with results of control and individual STPP or trehalose soaking treatments. The histological structure analysis revealed the permeated STPP/trehalose distinctly inhibited the dissociation of muscle fibers and reduced physical damage to connective tissues during storage. Furthermore, analysis of the thermal properties indicated STPP/trehalose treatment increased the Tg’ values of shrimp muscle tissues, likely by restricting the mobility of water molecules in muscle tissues and embedding proteins in the glassy matrix. Thus, the physical destruction caused by ice crystal growth was greatly reduced, due to the absence of water molecules around muscle proteins during frozen storage. Accordingly, the combined STPP/trehalose-VP treatment significantly enhanced the stability of frozen shrimp, and the results support the application of traditional cryoprotective additives. The treated shrimp can be stored at comparatively higher temperatures with limited physicochemical reactions during frozen storage.
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高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Chuan-hai Tu,Xue-er Qi,Shan-shan Shui,Hui-min Lin,Soottawat Benjakul,Bin Zhang
The oxidative effects of hydroxyl radical on the alterations of lipid profiles were investigated in shrimp muscle. Chemical results indicate peroxide value (PV) and thiobarbituric acid index (TBA-i) value in oxidation-treated shrimp significantly increased with oxidation time, and hydroxyl radical concentration increased, compared with those of in fresh samples. It was assumed that radical attack might induce lipid decomposition, backbone cleavage, and/or side-chain modifications. LC/MS-based lipidomics analysis revealed 835 lipids in shrimp assigned to 27 lipid classes, including 219 PCs and 98 CLs. In total, 86 and 34 differentially abundant lipids (DALs) accumulated at lower and higher levels, respectively, were identified in OS, compared with that in FS. This indicates hydroxyl radical attack altered the lipidomics profiles of shrimp muscle to a large extent. Furthermore, DALs, including CL 62:2, PC 38:3, and PE 34:9, could be considered as promising biomarkers to distinguish fresh and oxidation-treated shrimp products.
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AQUACULTURE
·
2013
·
IF 2.009
Shi-wei Xie,Li-xia Tian,Yan Jin,Hui-jun Yang,Gui-ying Liang,Yong-jian Liu
An 8-week feeding trial was conducted to evaluate the optimum dietary glycine level in low fishmeal diet for juvenile Pacific white shrimp , Litopenaeus vannamei . Six practical diets (44.4% protein, 8.8% lipid) were formulated to contain graded levels (2.26, 2.33, 2.44, 2.58, 2.67 and 2.74%) of l -glycine. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.61 g) and the shrimps were fed 4 times a day to apparent satiation. The results indicated that the weight gain and specific growth rate were significantly increased with the supplementation of glycine. The highest weight gain and specific growth rate were observed at 2.56% dietary glycine, and then decrease with a further increase in dietary glycine. Hepatosomatic index , protein productive value and condition factor were also significantly influenced by the dietary glycine levels and show a similar trend to those of growth performance. There were no significant differences observed in feed efficiency , protein efficiency ratio and survival among all treatments. Mg, Ca and Fe concentrations in whole body were significantly increased with the supplementation of glycine. Non-essential amino acid, essential amino acid and arginine ratio in muscle were significantly affected by the dietary glycine levels. Dietary glycine levels higher than 2.44% significantly increased the survival of shrimps after 2 h and 3 h of acute salinity change, and T-SOD activity and Na + /K + -ATPase activity in hematological and hepatopancreas before and after acute salinity change are also significantly affected by the dietary glycine levels. Analysis by second-order regression of weight gain indicated that the optimal dietary glycine level was 2.54% of the dry diet (5.7% of the dietary protein and 6.66% of the dietary amino acids).
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BRITISH JOURNAL OF NUTRITION
·
2026
·
IF 3.1
Xin Yu,Renzhi Liu,Futao Jing,Zhengxu Chen,Mingxiang Lu,Yaobing Li,Luqing Pan
Dietary plant-derived bioactive compounds for enhancing physiological health are becoming a prevalent strategy for antibiotic alternatives. Our study revealed the effects and underlying mechanism of Osthole (OST) and OST-Tetramethylpyrazine (TMP) compound in Litopenaeus vannamei based on network pharmacology, molecular docking and a 42-day feeding trial verification. The results illustrated that OST and OST-TMP compound significantly improved the survival rate (SR), weight gain rate (WGR), specific growth rate (SGR) and feed conversion ratio (FCR), strengthening the growth performance of L. vannamei. Meanwhile, combining the predictive results from network pharmacology and molecular docking, we propose that OST and TMP synergistically enhance the antioxidant defense capacity of shrimp through the synergistic Nrf2 signaling pathway, thereby enhancing the expression of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Furthermore, OST-TMP exhibited a significant increase of the immune response in hemocyte and intestine of shrimp, increasing the expression of antimicrobial peptide and lysozyme, and suppressing the inflammatory factors, via the synergistic (NF-κB) and complementary targets predicted by network pharmacology. Additionally, gut microbiota composition of L. vannamei was improved, and the dominant genera were correlated with intestinal immune in the compound groups. For the first time, we elucidated the mechanism of plant-derived bioactive compounds mediating physiological health in aquatic animals via a new strategy of network pharmacology-molecular docking-experimental verification, and identified the optimal addition amount of OST-TMP in shrimp (150 mg/kg TMP + 20 mg/kg OST), providing a technical safeguard for the animal health and the safety of aquatic products.
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BMC Veterinary Research
·
2026
·
IF 2.6
Qihang Liang,Qi Wang,Pinqi Chi,Depeng Fan,Beiping Tan,Shiwei Xie,Junming Deng,Hongyu Liu,Ling Zeng
Antimicrobial peptide BmKn2-7, a rationally optimized derivative of the scorpion venom peptide BmKn2, shows strong potential as an antibiotic alternative for controlling Vibrio parahaemolyticus in aquaculture. This study evaluated its antibacterial mechanisms and in vivo efficacy in Litopenaeus vannamei through biochemical assays, transcriptomic profiling, and challenge experiments. BmKn2-7 exhibited potent in-vitro activity (MIC = 125 µg/mL) and disrupted bacterial homeostasis by increasing membrane permeability, inducing oxidative stress, and inhibiting P-type ATPase. RNA-seq analysis revealed extensive transcriptional reprogramming, including the downregulation of quorum-sensing, virulence, and β-lactam resistance–associated genes. In vivo, BmKn2-7 significantly improved shrimp survival following V. parahaemolyticus infection and selectively reduced Vibrio abundance in the gut without compromising overall microbiota diversity. Together, these findings demonstrate that BmKn2-7 acts through complementary bactericidal and anti-virulence pathways while promoting intestinal microbial resilience, supporting its potential as a promising antimicrobial candidate for reducing vibriosis risk in shrimp aquaculture.
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FOOD HYDROCOLLOIDS
·
2019
·
IF 5.839
Jiahao Xu,Rongbian Wei,Zhe Jia,Ru Song
A biofunctional composite film was prepared using a casting method by incorporation of ε-polylysine (ε-PL) plus astaxanthin (Asta) extracts from Litopenaeus vannamei ( L. vannamei ) by-products into chitosan and gelatin film forming formulations. Compared with the bare gelatin or the chitosan-gelatin films, the composite film had similar water content and thickness, whereas, decreased water solubility, increased water vapour permeability and water contact angle. Molecular interactions were formed between gelatin and chitosan with or without ε-PL and Asta addition, as recorded and confirmed by X-ray diffraction and Fourier-transform infrared spectroscopy. The scanning electronic microscopy images showed rough cross-section with pores, as well as vertical cracks along the composite film matrix. Surface roughness decrease was observed for the composite film by atomic force microscopy . The composite film displayed strong inhibitory effect (near 100%) against spoilage bacteria derived from L. vannamei . Besides, the lipid oxidation of corn oil was retarded after addition of composite film, with less than 5 nmol/mL of malondialdehyde detected when incubation at 60 °C for 59 h. Therefore, such composite film could have potential applications for antibacterial and antioxidant food packaging material.
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Animal Nutrition
·
2024
·
IF 6.1
Menglin Shi,Haomin Li,Tianyu Chen,Bocheng Huang,Xiaoyue Li,Xiaohui Dong,Shuyan Chi,Qihui Yang,Hongyu Liu,Junming Deng,Beiping Tan,Shuang Zhang,Shiwei Xie
This experiment aimed to evaluate the impact of dietary hydroxyproline (Hyp) supplementation on the flesh quality of juvenile Pacific white shrimp ( Litopenaeus vannamei ) fed a low fishmeal diet. Six formulated diets included one high fishmeal (HF; 25% fishmeal content) and five low fishmeal diets (10% fishmeal content) with 0%, 0.2%, 0.4%, 0.6% and 0.8% Hyp (LF0, LF2, LF4, LF6 and LF8, respectively). Each diet was assigned to four replicates and 40 shrimp (0.32 ± 0.00 g) per replicate were fed four times a day for 8 weeks. Dietary Hyp supplementation had little effects on growth performance, but significantly increased the contents of Hyp, prolyl 4-hydroxylases (P4Hs), and collagen ( P < 0.05). The meat yield, springiness, hardness, chewiness, and cohesiveness of muscle were the highest in the LF2 group. Cooking loss and freezing loss of muscle were the lowest in the LF2 group ( P < 0.05). Dietary supplementation with 0.4% Hyp increased the myofiber density and decreased the myofiber diameter of muscle ( P < 0.05). Supplementation of Hyp in the diet up-regulated the mRNA expression of smyhc5 , smyhc15 , col1a1 , col1a2 , igf-1f , tgf-β and tor , and down-regulated the mRNA expression of smyhc 1 , smyhc 2 , smyhc 6a ( P < 0.05). Supplementation of Hyp in the diet up-regulated the protein expression of P-4E-BP1, P-AKT, AKT and P-AKT/AKT ( P < 0.05). These results suggested that the addition of 0.4% Hyp to low fishmeal diets improved the flesh quality of L. vannamei .
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AQUACULTURE
·
2020
·
IF 3.225
Weiliang Guan,Wenqian Nong,Xiaobo Wei,Minqi Zhu,Linchun Mao
A novel strategy of water-free transportation (WFT) for live shrimp was recently developed. In this study, survival rate, hormone response, free radical content, oxidation in lipids and proteins, and flesh quality were evaluated after WFT. Meanwhile water transportation (WT) was employed as a reference for comparison. Results showed only 5.1% mortality after 10 h of WFT, while crustacean hyperglycemic hormone (CHH), reactive nitrogen species (RNS), free fatty acid content (FFA), peroxide value (PV), carbonyl concentration, intrinsic fluorescence intensity (IFI), and surface hydrophobicity significantly increased. Myosin heavy chain (MHC) and actin degradation were also detected after WFT. Although oxidation occurred in lipids and proteins, no significant changes in texture and water-holding capacity (WHC) were detected after WFT or WT ( p > 0.05). The 10 h of WFT showed little influence on flesh quality of shrimp even though lipids and proteins oxidation occurred during the mimicked water-free transportation.
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高影响因子
AQUACULTURE NUTRITION
·
2015
·Vol. 23
·期 1
·54-62
·
IF 1.395
S.-W. Xie,Y.-T. Li,W.-W. Zhou,L.-X. Tian,Y.-M. Li,S.-L. Zeng,Y.-J. Liu
An 8-week feeding trial was conducted to evaluate the optimum dietary γ-aminobutyric acid (GABA) level in low fishmeal diet for juvenile Pacific white shrimp, Litopenaeus vannamei . Six practical diets (449 g kg −1 protein, 87 g kg −1 lipid) supplemented with graded levels of GABA (0, 50, 100, 150, 200, 250 mg kg −1 ) were formulated. Each diet was randomly assigned to triplicate groups of 30 shrimps (approximately 0.44 g), and the shrimps were fed four times a day to apparent satiation. Weight gain and gain rate were significantly increased with the supplementation of GABA ( P < 0.05). Hepatosomatic index and survival were also significantly influenced by the dietary GABA levels ( P < 0.05) and show a similar trend to those of growth performance. Insulin and neuropeptide Y concentrations in blood were increased with the supplementation of GABA. In total, 150 mg GABA kg −1 -supplemented diets significantly increased the survival after 12, 24 and 36 h of NH 3 stress, also increased the total antioxidant capacity, total superoxide dismutase, catalase, antihydroxyl radical and phenoloxidase activities before and after the 36 h NH 3 stress compared to basic group. These results clearly indicated that GABA could improve growth performance, antioxidative capacity and resistance against NH 3 stress in L. vannamei , and 150 mg kg −1 GABA supplementation was suitable for L. vannamei fed with low fishmeal diet.
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JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Shuangshuang Zhao,Yingying Bian,Gewen Zhang,Guang Yang,Xiaoyue Hou,Jiajin Gui,Shuting Mu,Shu Liu,Yaowei Fang
Seafood is highly perishable and has a short shelf-life. This study investigated the effect of chitosan and alginate (CH-SA) coating combined with the cell-free supernatant of Streptococcus thermophilus FUA329 (CFS) as a preservative on the quailty of white shrimp (Litopenaeus vannamei) refrigerated at 4° for 0, 3, 6, 9, 12, 15 days. Freshly shrimps were randomly divided into four groups: the CFS group (400 mL); the CH-SA group (1% chitosan/1% alginate); the CFS-CH-SA group (1% chitosan/1% alginate with 400 mL CFS) are treatment groups, and the control group (400 mL sterile water). The CFS–CH–SA coating effectively suppressed microbial growth total viable count and chemical accumulation (pH, total volatile basic nitrogen, thiobarbituric acid reactive substance) compared with the control. Additionally, the CFS–CH–SA coating improved the texture and sensory characteristics of shrimp during storage. The coated shrimp exhibited significantly reduced water loss ( p < 0.05). The combination of CH–SA coating with CFS treatment can extend the shelf life of shrimp. Practical Application Recently, edible films have received more consideration as a promising method to enhance the shelf life of seafood. The presence of Lactic acid bacteria metabolites in edible films reduces spoilage and improves consumer health. Our findings encourage the application of edible coating incorporated with cell-free supernatant of Streptococcus thermophilus FUA 329 to design multifubctional foods and preserve the qualities of shrimp.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Shujun Lei,Yuyan Ding,Min Hu,Long Jiao,Bin Zhang,Chuandong Fang
Freeze-thaw cycles amplify species-specific stability differences of shrimp MPs. • Parapenaeopsis hardwickii MPs: compact native state but high freeze-induced damage. • Litopenaeus vannamei MPs maintain superior structural integrity under frozen stress. • Results highlight the necessity of stable frozen environments for shrimp preservation. This study compared the structural and functional responses of myofibrillar proteins (MPs) from Litopenaeus vannamei (LV, farmed species) and Parapenaeopsis hardwickii (PW, wild species) to long-term frozen storage (−20 °C, FG) and freeze-thaw cycles (FC), aiming to reveal species-specific freezing sensitivity mechanisms and guide differentiated preservation strategies. Results indicated that FC induced more severe damage than FG, leading to greater oxidation (PW: hydrophobicity +29.12%, carbonyl +2.53 nmol/mg), structural unfolding (LV: α -helix −4.96/−5.21%, β -sheet +2.82/+6.93% in FG/FC; PW: α -helix −7.10/−8.17%, β -sheet +8.15/+9.27%), aggregation, and loss of solubility and emulsifying functionality. PW MPs demonstrated higher freezing sensitivity compared to LV MPs, characterized by less stable secondary/tertiary structures, faster oxidation and aggregation, and consequently a more pronounced decline in functional properties. These findings not only clarify the molecular damage pathways of shrimp MPs under frozen stress but also provide actionable guidance for industrial preservation, facilitating the quality maintenance of different shrimp products. Download: Download high-res image (253KB) Download: Download full-size image
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