FOOD CHEMISTRY
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2026
·
IF 10.4
Xin Ma,Shimin Wu
Parent and oxygenated polycyclic aromatic hydrocarbons (PAHs and OPAHs) pose a threat to public health; however, few studies have reported strategies to mitigate their formation during baking, particularly for OPAHs. This study aimed to inhibit the formation of PAHs and OPAHs in baking systems using myricetin (Myr)-loaded ethyl cellulose (EC)/carboxymethyl cellulose (CMC) nanoparticles. The nanoparticles improved the water solubility and antioxidant activity of Myr. Molecular dynamics simulations revealed that the binding affinities followed the order: EC-Myr > CMC-Myr > EC-CMC. In baking systems, the nanoparticles effectively reduced the concentrations of 24 PAHs and 12 OPAHs, exhibiting stronger inhibitory effects than free myricetin. GC–MS/MS and electron paramagnetic resonance analyses revealed that CMC and Myr synergistically suppressed PAHs and OPAHs formation by reducing intermediates and unsaturated carbon-centered radicals. Overall, this study first provided a novel approach to reducing the concentrations of PAHs and OPAHs and their associated health risks in baking systems.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
·
IF 3.1
Xiangjun Chen,Yue Wang,Pinzheng Li,Jianbiao Yao,Yuanzheng Song,Huidi Jiang,Su Zeng,Lingfang Chen,Hui Zhou
The total flavone of Abelmoschi Corolla (TFA) has diverse pharmacological effects, including anti-inflammatory and antioxidant properties, and improving insulin resistance. Clinically, TFA has been utilized for a substantial duration for managing various renal diseases. Phase II metabolites, such as sulfated and glucuronidated conjugates, are the primary in vivo forms of flavonoids and may mediate the pharmacological effects of TFA. However, the direct quantification of conjugated metabolites is limited by the lack of reference standards. To address this, β-glucuronidase/sulfatase hydrolysis can be used to cleave these conjugates, releasing aglycones and enabling the indirect quantification of total flavonoids. In this study, a reliable and sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the quantification of gossypetin, myricetin, quercetin, and isorhamnetin in mouse plasma following enzymatic hydrolysis. Chromatographic separation was performed using a Waters ACQUITY UPLC HSS-T3 column with gradient elution. The mobile phase consisted of acetonitrile and water (both containing 0.1 % formic acid) at a flow rate of 0.35 mL/min. The method was validated for plasma analysis and successfully applied to a pharmacokinetic study in mice following oral administration of TFA. The results showed that the analyzed aglycones rapidly attained maximum plasma concentrations. The area under the curve for each compound demonstrated a positive correlation with the administered dose in the range of 100–400 mg/kg. Moreover, the isorhamnetin concentration increased significantly following 7-day repeated gavage compared to that with a single 200 mg/kg TFA dose. These pharmacokinetic findings may inform future drug development and clinical applications of TFA-derived substances.
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MOLECULES
·
2023
·Vol. 28
·期 2
·758
·
IF 4.6
Hao-Jie Bai,De-Yan Qi,Hong-Wei Li,Yuqing Wu
A significant emission enhancement (>100-fold) of glutathione-capped bimetallic gold and copper nanoclusters (AuCuNC@GSH) was achieved by assembling with Al3+ions and by assembly-induced emission enhancement (AIEE). Further chelation of myricetin to Al3+resulted in emission quenching of AuCuNC-Al3+, which was applied to specifically detect myricetin. Two linear responses were shown in the range of 0–1.5 μM and 1.5–50 μM, separately, leading to a low limit of detection at 8.7 nM. The method was successfully and accurately applied to myricetin determination in grape juice, which showed good application for real samples. Finally, the in-depth mechanism revealed that both the chelation of myricetin and Al3+and the inner filter effect (IFE) between myricetin-Al3+and AuCuNC-Al3+greatly contributed to the quenching response of myricetin. Therefore, the present study provides an easy way to improve the fluorescence property of metal nanoclusters. Additionally, it supplies a cost-effective and easily performed approach to detect myricetin with high selectivity and sensitivity.
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MICROCHEMICAL JOURNAL
·
2020
·
IF 3.594
Lizhen Liu,Zhi Mi,Ziying Guo,Junling Wang,Feng Feng
In this research, a novel label-free fluorescent sensor for the determination of myricetin was designed based on carbon quantum dots (CQDs). The CQDs were prepared via green and straightforward microwave-assisted heating method using aspartic acid and urea as precursors. The prepared CQDs showed strong fluorescence and good solubility in water. The sensing principle of sensor is the quenching of CQDs fluorescence by myricetin via inner filter effect (IFE). The developed CQDs sensor was sensitive to myricetin with a wide linear detection range of 1 to 80 µM and a low limit of detection (LOD) of 18.4 nM. The potential interfering substances showed no influence on the determination of myricetin. Furthermore, the proposed sensor based on CQDs was successfully applied for the determination of myricetin in red wine samples and exhibited simple operation, low cost and high accuracy. The results indicated our developed sensor based on CQDs has great application prospects for myricetin detection in real sample analysis.
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Journal of Pharmaceutical Analysis
·
2019
·
IF 4.44
Satar Tursynbolat,Yrysgul Bakytkarim,Jianzhi Huang,Lishi Wang
The simultaneous electrochemical determination of myricetin and rutin remains a challenge due to their indistinguishable potentials. To solve this problem, we constructed a ternary platinum nanoparticle, reduced graphene oxide, multi-walled carbon nanotubes (Pt@r-GO@MWCNTs) nanocomposite via a facile one-pot synthetic method. Under the optimized conditions, the ternary Pt@r-GO@MWCNTs nanocomposite exhibited good electrocatalytic activity toward myricetin and rutin via solid phase extraction and excellent performance for the simultaneous determination of myricetin and rutin. The oxidation peak current of myricetin was proportional to its concentrations in the range of 0.05–50 μM with a detection limit of 0.01 μM (S/N = 3). The linear range for rutin was 0.05–50 μM with a detection limit of 0.005 μM (S/N = 3). The ternary nanocomposite sensor also exhibited good reproducibility and stability, and was successfully used for the simultaneous determination of myricetin and rutin in real orange juice samples with recoveries ranging between 100.57% and 108.46%.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2025
·
IF 3.5
Cheng Zhang,Shuangling Zhang,Jingjun Ma,Xiaohui Wang,Min Chen,Liangtao Lv,Heping Liu,Guanyu Zhao,Junpeng Li,Nan Sun
BACKGROUND The limited water solubility and instability of myricetin (Myr) restrict its application in functional food. Peptides from colla corii asini (CCAP) have recognized potential for the design of hybrid nanoparticles due to their therapeutic bioactivity. In this study, a dual-protein (animal–plant hybrid) delivery system was developed by the integration of CCAP with Zein (Z), with the objective of enhancing the efficiency of Myr for diabetes management. RESULTS Size-exclusion chromatography combined with multi-angle light scattering and zeta potential revealed small- and medium-sized CCAP fragments (83.0–100.5 and 100.5–785.6 Da, 10.0% and 80.0%, respectively; degree of hydrolysis: 78.3%) enabling electrostatic interactions with Z. CCAP-Z-Myr nanoparticles (98.2 nm) exhibited enhanced encapsulation efficiency (91.4%) and thermal stability (endothermic peak shift from 71.8 °C (Z-Myr) to 138 °C (CCAP-Z-Myr)). Fourier transform infrared spectroscopy and X-ray diffraction confirmed Myr amorphization (disappearance of crystalline peaks at 5.8°, 11.4°, 14.1°, 16.6°, 26.3° and 28.4°) and electrostatic interactions between CCAP and Z (CO peak shift from 1657 cm −1 (Z) to 1633 cm −1 (CCAP-Z), amide II from 1542 cm −1 (Z) to 1536 cm −1 (CCAP-Z)). Antioxidant activity of CCAP-Z-Myr surpassed that of Z-Myr (ABTS: 60.4% versus 25.3%, DPPH: 57.6% versus 33.0%, FRAP: 0.65 versus 0.42, hydroxyl radicals: 50.4% versus 30.2%, superoxide anions: 50.9% versus 29.7%). α -Glucosidase inhibitory activity of CCAP-Z-Myr was notably improved compared with Z-Myr (78.5% versus 64.8%). CONCLUSION This study demonstrated a dual animal–plant protein strategy to enhance hydrophobic bioactive Myr delivery for diabetes management, offering novel insights for functional foods and therapeutic formulations. © 2025 Society of Chemical Industry.
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Innate Immunity
·
2024
·
IF 2.8
Yiqin Shu,Rui Yang,Huijie Wen,Qiannan Dong,Zhiqi Chen,Yang Xiang,Hao Wu
Rheumatoid arthritis (RA) is a chronic disease characterized by joint inflammation and severe disability. However, there is a lack of safe and effective drugs for treating RA. In our previous study, we discovered that myricetin (MC) and celecoxib have a synergistic effect in the treatment of RA. We conducted in vitro and in vivo experiments to further investigate the effects and mechanisms of action of MC. Our findings demonstrated that MC treatment effectively reduced the release of neutrophil extracellular traps (NETs) and alleviated the inflammatory response in RA. Mechanistic studies showed that MC prevents the entry of PADI4 and MPO into the cell nucleus, thereby protecting DNA from decondensation. In a rat arthritis model, MC improved histological changes in ankle joints and suppressed NET-related signaling factors. In conclusion, MC protects the ankle joints against arthritis by inhibiting MPO and PADI4, thereby reducing NET release. The pharmacological mechanism of MC in RA involves the inhibition of NET release.
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FOOD CHEMISTRY
·
2026
·
IF 10.4
Luyan Liu,Kaiyue Sun,Xin Xie,Yanghui Chen,Jiahui Wang,Yaxin He,Junjie Yu,Meng Huang,Yan Lin,Juan Chen,Jian Liu,Xian Zhang
Thermal processing promotes advanced glycation end products (AGEs) generation in food. Dietary flavonoids possess anti-oxidative and anti-glycation activities. This study aimed to investigate the effect and underlying mechanism of different pattern diets and flavonoids on AGEs production and AGEs-induced intestinal damage. AGEs production was assessed in chow diet (CD), high-fat diet (HFD), high-fat/high-cholesterol western diet (WD) and flavonoid-supplemented diet during thermal processing and simulated digestion. AGEs-induced intestinal damage and the protective effect of myricetin were evaluated in Caco-2 cells. During thermal processing and simulated digestion, HFD and WD generated more AGEs, N ε -carboxymethyl-lysine and N ε -carboxyethyl-lysine than CD. Flavonoids inhibited AGEs production, with myricetin exhibiting the highest activity. Dietary AGEs impaired intestinal epithelial nutrient transport and barrier function, and promoted intracellular ROS generation through inhibiting Nrf2/Catalase pathway, while myricetin reversed this effect. Altogether, dietary myricetin prevents thermal processing-induced AGEs formation in different pattern diets and mitigates the associated intestinal epithelial damage.
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Food Bioscience
·
2024
·
IF 4.8
Yifan Ping,Yang Yang,Hongfei Gao,Xiuwei Liu,Jun Pan,Ke Zhang,Huiyan Gu,Hao Tian,Lei Yang
In this study, three different target analytes from the fruit of myrtle ( Rhodomyrtus tomentosa ) were extracted via an enzymatic-homogenate synergistic extraction (EHSE) process, and 10 different aqueous solutions of natural surfactants were selected as extraction solvents, thus enabling the simultaneous extraction of piceatannol, resveratrol, and myricetin from the fruit of myrtle ( Rhodomyrtus tomentosa ). Important factors affecting the yield of piceatannol, resveratrol, and myricetin, including enzyme type, enzyme concentration, pH, surfactant type and dosage, homogenization time, liquid‒solid ratio, incubation and extraction time, incubation time and extraction temperature, were optimized via single-factor experiments. Three important parameters were optimized via the Box‒Behnken design: the liquid‒solid ratio, the incubation–extraction time, and the reaction temperature. The final optimum extraction process conditions were as follows: β-glucosidase concentration, 3.0%, homogenate; time, 3 min; pH, 5; tea saponin concentration, 0.8%; liquid‒solid ratio, 45 mL/g; incubation–extraction time, 2.5 h; and temperature, 67.5 °C. With the optimized experimental parameters, the piceatannol, resveratrol, and myricetin yields were 742.12 ± 37.11 μg/g, 18.58 ± 0.94 μg/g, and 24.83 ± 1.25 μg/g, respectively. Methodological validation and comparison experiments with traditional solvents demonstrated that EHSE is a successful, green, and safe method for extracting active plant components. This approach employs an ecologically benign water-based solution of tea saponin as the extraction solvent, and it combines enzymatic and homogenate procedures to increase extraction efficiency. This study provides a reasonable basis for the application and research of synergistic extraction technology and suggests that more diverse solvent choices should be considered.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2026
·
IF 5.7
Songbin He,Zhiqi Lin,Xiaojing Zhou,Qiushi Wang,Chaohua Fang,Qinghao Jin,Liping Guan
Objective The mixed particles of Myricetin (MYR)/Chitooligosaccharide (COS)/Astaxanthin (AST) had not study to therapeutic effects on Alzheimer's disease (AD) combined with depression. In this study, the mixed particles of MYR/COS/AST were investigate the inhibitory activities against cholinesterase (ChE) and monoamine oxidase (MAO), possessing good activity were further assayed to inhibit β-amyloid1-42 (Aβ 1-42 ) aggregation, beta-site amyloid precursor protein cleaving enzyme-1 (BACE-1), the neuroprotective activity and cytotoxicity. Materials and methods ChE and MAO inhibitory activities by Ellman and Holts method. Aβ aggregation were evaluated by thioflavin T assay, BACE1 inhibition used the fluorescence resonance energy transfer (FRET)-based. The protective effect were tested by against L-Glutamate (L-Glu)-induced HT22 cell damage, Cu 2+ - and Fe 3+ -induced neuronal damage, the cytotoxicity were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. The antidepressant activities were measured by the forced swimming test (FST). Results The results showed that the mass ratio of the mixed particles MYR/COS/AST was 10:10:3, which exhibited the best inhibitory activities on AChE, MAO, also exhibited inhibition against Aβ 1-42 aggregation, BACE-1, Aβ 1-42 disaggregation, exerted protective effects L-Glu-induced HT22 cell damage and on the cells against Cu 2+ and Fe 3+ -induced damage in BV-2 cells. In the FST, the mixed particles of MYR/COS/AST (10:10:1) exhibited good antidepressant effect. None of three substances showed cytotoxicity against L929 cells. The molecular docking revealed important interactions between the active three substances and amino acid residues. Conclusions These studies provide the technical data for ensuring potential treatment of AD combined with depression of the mixed particles of MYR/COS/AST (10:10:3).
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 9
·1-10
·
IF 5.705
Liu Chaoqiao,Huang Jianzhi,Wang Lishi
The authors describe a method for electrochemical synthesis of a nanocomposite consisting of carboxylated multi-walled carbon nanotubes, polythionine and platinum nanoparticles (denoted as cMWCNT@pTh@Pt). The composite was placed on a glassy carbon electrode (GCE) to obtain a sensor for simultaneous determination of myricetin and rutin by differential pulse voltammetry, with typical peak voltages of 0.16 and 0.34 V (vs. SCE). Under optimal conditions, the modified GCE has a linear response in the range of 0.01–15 μM myricetin concentration range, and a 3 nM lower detection limit. For rutin, the data are 0.01–15 μM and 1.7 nM, respectively. The sensor is selective, stable and reproducible. It was successfully applied to the simultaneous determination of myricetin and rutin in spiked juice samples, and satisfactory results were obtained.
A nanocomposite consists of carboxy multi-walled carbon nanotube (cMWCNT), polythionine (pTh) and platinum nanoparticles (Pt) was presented for the simultaneous determination of myricetin and rutin. The nanocomposite shows excellent electrochemical determination performances based on a synergistic effect among cMWCNT, pTh and Pt.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Long Ma,Xiuqi Cao,Haiyue Wang,Kui Lu,Ying Wang,Chunhao Tu,Yujie Dai,Yuanyuan Meng,Yuyin Li,Peng Yu,Shuli Man,Aipo Diao
Human flap endonuclease 1 (hFEN1) is instrumental in DNA replication and repair. It is able to cleave the 5′ single-stranded protrusion (also known as 5′ flap) resulting from strand displacement reactions. In light of its crucial functions, hFEN1 is now deemed as a nontrivial target in the DNA damage response system for anticancer drug development. Herein, we report that myricetin and some natural flavonoids are able to inhibit hFEN1. Structure–activity relationship, inhibitory mechanisms, molecular docking, and cancer cell-based assays have been performed. Our original findings expand the activity of flavonoids and may pave the way for flavonoid-assisted targeted cancer therapy.
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